Quantifying stakeholder understanding of an ecosystem service trade-off

Sustainable management of global natural resources is challenged by social and environmental drivers, adding pressure to ecosystem service provision in many regions of the world where there are competing demands on environmental resources. Understanding trade-offs between ecosystem services and how they are valued by different stakeholder groups is therefore critical to maximise benefits and avoid conflict between competing uses. In this study we developed a novel participatory trade-off experiment to elicit the perception of 43 participants, from across four key stakeholder groups, working in land and water management (Environmental Regulators, Farming Advisors, Water Industry Staff and Catchment Scientists). Using the Production Possibility Frontier (PPF) concept, we quantified stakeholder assessment of both the shape and the uncertainty around the PPF in a trade-off between agricultural intensity and the ecological health of freshwater systems. The majority of stakeholder groups selected threshold and logistic decay trade-off curves to describe the relationship of the trade-off, and estimated the uncertainty around the curves to be intermediate or large. The views of the four stakeholder groups differed significantly regarding how they estimated stakeholder trade-off prioritisation; the largest difference in perspectives was identified between Environmental Regulators and Farm Advisors. The methodology considered the cultural, socio-economic and institutional specificities of an ecosystem service interaction and identified potential sources of conflict but also possible solutions for win-win opportunities to explore and share understanding between stakeholders. Valuing stakeholder knowledge as a form of expert data and integrating this into participatory decision-making processes for land and water management thus contributes considerable value beyond traditional approaches to ecosystem service assessments

Rapid Characterisation of Stakeholder Networks in Three Catchments Reveals Contrasting Land-Water Management Issues

Catchments are socio-ecological systems integrating land, water and people with diverse roles and views. Characterising stakeholder networks and their levels of influence and interaction within catchments can help deliver more effective land and water management. In this study, we combined stakeholder analysis and social network methods to provide a novel stakeholder-mapping tool capable of identifying interactions among the land and water management communities across three contrasting study catchments. The overarching aim was to characterise the influence of different stakeholders involved in catchment management based on the perceptions of participants from four key stakeholder groups (Environmental Regulators, Water Industry Practitioners, the Farm Advisor Community, and Academics). A total of 43 participants identified 28 types of specific catchment management stakeholder groups with either core or peripheral importance to our three case study catchments. Participants contributed 490 individual scores relating to the perceived influence of these different stakeholder groups and categorised whether this influence was positive, negative or neutral for the management of catchment resources. Local Government, Farmers and Environmental Regulators were perceived to have the greatest level of influence. Social network analysis further determined which stakeholders were most commonly connected in all of the study catchments and hence formed the core of stakeholder networks in each catchment. Comparing outputs from the analysis of three contrasting river catchments, as well as between participants from four key stakeholder groups allowed identification of which stakeholders were more central to the catchment management networks. Such analyses could help facilitate effective communication within land and water management stakeholder networks by targeting highly connected opinion leaders or promoting peer learning via distinct catchment subgroups

Catchment-Scale Participatory Mapping Identifies Stakeholder Perceptions of Land and Water Management Conflicts

Competing socioecological demands and pressures on land and water resources have the potential to increase land use conflict. Understanding ecosystem service provisioning and trade-offs, competing land uses, and conflict between stakeholder groups in catchments is therefore critical to inform catchment management and the sustainable use of natural resources. We developed a novel stakeholder engagement methodology that incorporates participatory conflict mapping in three catchments with a short questionnaire to identify the perceptions of 43 participants from four key land and water management stakeholder groups: environmental regulators, water industry practitioners, the farm advisor community, and academics. The participatory mapping exercise produced heat maps of perceived conflict and land use competition, providing spatial detail of the complex combination of land use issues faced by catchment managers. Distinct, localised hotspots were identified in areas under pressure from flooding, abstraction, and urbanisation; as well as more dispersed issues of relevance at the landscape scale, such as from farming, forestry, energy production, and tourism. Subsequent regression modelling linked perceived conflict to land cover maps and identified coastal, urban, and grassland areas as the most likely land cover types associated with conflict in the study catchments. Our approach to participatory conflict mapping provides a novel platform for catchment management and can facilitate increased cooperation among different catchment stakeholders. In turn, land and water management conflicts can be recognised and their underlying drivers and likely solutions identified in an effort to better manage competing demands on catchment resources

Cell functional enviromics: Unravelling the function of environmental factors

<p>Abstract</p> <p>Background</p> <p>While functional genomics, focused on gene functions and gene-gene interactions, has become a very active field of research in molecular biology, equivalent methodologies embracing the environment and gene-environment interactions are relatively less developed. Understanding the function of environmental factors is, however, of paramount importance given the complex, interactive nature of environmental and genetic factors across multiple time scales.</p> <p>Results</p> <p>Here, we propose a systems biology framework, where the function of environmental factors is set at its core. We set forth a "reverse" functional analysis approach, whereby cellular functions are reconstructed from the analysis of dynamic envirome data. Our results show these data sets can be mapped to less than 20 core cellular functions in a typical mammalian cell culture, while explaining over 90% of flux data variance. A functional enviromics map can be created, which provides a template for manipulating the environmental factors to induce a desired phenotypic trait.</p> <p>Conclusion</p> <p>Our results support the feasibility of cellular function reconstruction guided by the analysis and manipulation of dynamic envirome data.</p

A new generation of 99.999% enriched 28Si single crystals for the determination of Avogadro's constant

A metrological challenge is currently underway to replace the present definition of the kilogram. One prerequisite for this is that the Avogadro constant, NA, which defines the number of atoms in a mole, needs to be determined with a relative uncertainty of better than 2  ×  10−8. The method applied in this case is based on the x-ray crystal density experiment using silicon crystals. The first attempt, in which silicon of natural isotopic composition was used, failed. The solution chosen subsequently was the usage of silicon highly enriched in 28Si from Russia. First, this paper reviews previous efforts from the very first beginnings to an international collaboration with the goal of producing a 28Si single crystal with a mass of 5 kg, an enrichment greater than 0.9999 and of sufficient chemical purity. Then the paper describes the activities of a follow-up project, conducted by PTB, to produce a new generation of highly enriched silicon in order to demonstrate the quasi-industrial and reliable production of more than 12 kg of the 28Si material with enrichments of five nines. The intention of this project is also to show the availability of 28Si single crystals as a guarantee for the future realisation of the redefined kilogram

The Hidden Sexuality of Alexandrium Minutum: An Example of Overlooked Sex in Dinoflagellates

Dinoflagellates are haploid eukaryotic microalgae in which rapid proliferation causes dense blooms, with harmful health and economic effects to humans. The proliferation mode is mainly asexual, as the sexual cycle is believed to be rare and restricted to stressful environmental conditions. However, sexuality is key to explaining the recurrence of many dinoflagellate blooms because in many species the fate of the planktonic zygotes (planozygotes) is the formation of resistant cysts in the seabed (encystment). Nevertheless, recent research has shown that individually isolated planozygotes in the lab can enter other routes besides encystment, a behavior of which the relevance has not been explored at the population level. In this study, using imaging flow cytometry, cell sorting, and Fluorescence In Situ Hybridization (FISH), we followed DNA content and nuclear changes in a population of the toxic dinoflagellate Alexandrium minutum that was induced to encystment. Our results first show that planozygotes behave like a population with an “encystment-independent” division cycle, which is light-controlled and follows the same Light:Dark (L:D) pattern as the cycle governing the haploid mitosis. Resting cyst formation was the fate of just a small fraction of the planozygotes formed and was restricted to a period of strongly limited nutrient conditions. The diploid-haploid turnover between L:D cycles was consistent with two-step meiosis. However, the diel and morphological division pattern of the planozygote division also suggests mitosis, which would imply that this species is not haplontic, as previously considered, but biphasic, because individuals could undergo mitotic divisions in both the sexual (diploid) and the asexual (haploid) phases. We also report incomplete genome duplication processes. Our work calls for a reconsideration of the dogma of rare sex in dinoflagellates.Versión del edito

Dynamics of Alexandrium fundyense blooms and shellfish toxicity in the Nauset Marsh System of Cape Cod (Massachusetts, USA)

Author Posting. © The Author(s), 2011. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Harmful Algae 12 (2011): 26–38, doi:10.1016/j.hal.2011.08.009.Paralytic Shellfish Poisoning (PSP) toxins are annually recurrent along the Massachusetts coastline (USA), which includes many small embayments and salt ponds. Among these is the Nauset Marsh System (NMS), which has a long history of PSP toxicity. Little is known, however, about the bloom dynamics of the causative organism Alexandrium fundyense within that economically and socially important system. The overall goal of this work was to characterize the distribution and dynamics of A. fundyense blooms within the NMS and adjacent coastal waters by documenting the distribution and abundance of resting cysts and vegetative cells. Cysts were found predominantly in three drowned kettle holes or salt ponds at the distal ends of the NMS - Salt Pond, Mill Pond, and Town Cove. The central region of the NMS had a much lower concentration of cysts. Two types of A. fundyense blooms were observed. One originated entirely within the estuary, seeded by cysts in the three seedbeds. These blooms developed independently of each other and of the A. fundyense population observed in adjacent coastal waters outside the NMS. The temporal development of the blooms was different in the three salt ponds, with initiation differing by as much as 30 days. These differences do not appear to reflect the initial cyst abundances in these locations, and may simply result from higher cell retention and higher nutrient concentrations in Mill Pond, the first site to bloom. Germination of cysts accounted for a small percentage of the peak cell densities in the ponds, so population size was influenced more by the factors affecting growth than by cyst abundance. Subsurface cell aggregation (surface avoidance) limited advection of the vegetative A. fundyense cells out of the salt ponds through the shallow inlet channels. Thus, the upper reaches of the NMS are at the greatest risk for PSP since the highest cyst abundances and cell concentrations were found there. After these localized blooms in the salt ponds peaked and declined, a second, late season bloom occurred within the central portions of the NMS. The timing of this second bloom relative to those within the salt ponds and the coastal circulation patterns at that time strongly suggest that those cells originated from a regional A. fundyense bloom in the Gulf of Maine, delivered to the central marsh from coastal waters outside the NMS through Nauset Inlet. These results will guide policy decisions about water quality as well as shellfish monitoring and utilization within the NMS and highlight the potential for “surgical” closures of shellfish during PSP events, leaving some areas open for harvesting while others are closed.This work was supported by NOAA Grant NA06OAR4170021, NPS Grant H238015504 and by the Woods Hole Center for Oceans and Human Health through NSF Grants OCE-0911031 and OCE-0430724 and NIEHS Grant 1P50-ES01274201. B.G.C. was supported by a Xunta de Galicia Ángeles Alvariño fellowship and the Stanley W. Watson Chair for Excellence in Oceanography under a Postdoctoral program at the Woods Hole Oceanographic Institution