A short-term comparison of wheat straw and poplar wood chips used as litter in tiestalls on hygiene, milk, and behavior of lactating dairy cows

A short-term study was conducted to compare the effect of using poplar wood chips (PWC) instead of wheat straw (WS) litter in dairy cows. A total of 38 lactating Holstein cows (204 ± 119 days in milk, 26.9 ± 6.5 kg of milk yield [MY]) were housed in a tiestall farm for a 10-d trial including 5 d of adaptation followed by 5 sampling days (from d 5 to 10). Cows were divided into 2 homogeneous groups: one group was bedded with WS, and the second with PWC. Both litter materials were provided in the amount of 7 kg/stall per d. Each group was composed of 3 subgroups of 6 or 7 cows; the subgroups were physically separated along the feeding line by wooden boards. During the sampling days, fecal composition, used litter composition, and bacterial count (Clostridium spp., Salmonella spp., Escherichia coli, Lactobacillus, and total bacterial count) were analyzed by subgroup twice a day. On d 1 and from d 5 to 10, udder hygiene score and cow cleanliness score were also evaluated individually twice a day. Meanwhile MY, milk hygiene (total bacterial count [TBC], coliform bacterial count [CBC], and spore-forming unit [SFU]) and quality were measured and analyzed from 9 animals per group. Moreover, individual animal behavior (body position and behavioral traits) and subgroup dry matter intake were measured on d 9 and 10. Fecal dry matter did not differ between groups, PWC had the lowest used litter moisture and N content favoring the highest clean cow frequency, but also gave rise to the greatest used litter microbial contamination. The MY, milk quality, TBC, SFU, and CBC were similar. The lying behavior frequency was similar between groups. However, the PWC group showed the lowest sleeping frequency, the highest frequency of other behaviors (including discomfort signs), and the lowest dry matter intake. However, despite this apparent reduction in cow comfort, no biologically important differences were observed in this short-term study between cows on PWC and WS in milk production or hygiene

Survey on Carbapenem-Resistant Bacteria in Pigs at Slaughter and Comparison with Human Clinical Isolates in Italy

This study is focused on resistance to carbapenems and third-generation cephalosporins in Gram-negative microorganisms isolated from swine, whose transmission to humans via pork consumption cannot be excluded. In addition, the common carriage of carbapenem-resistant (CR) bacteria between humans and pigs was evaluated. Sampling involved 300 faecal samples collected from slaughtered pigs and 300 urine samples collected from 187 hospitalised patients in Parma Province (Italy). In swine, MIC testing confirmed resistance to meropenem for isolates of Pseudomonas aeruginosa and Pseudomonas oryzihabitans and resistance to cefotaxime and ceftazidime for Escherichia coli, Ewingella americana, Enterobacter agglomerans, and Citrobacter freundii. For Acinetobacter lwoffii, Aeromonas hydrofila, Burkolderia cepacia, Corynebacterium indologenes, Flavobacterium odoratum, and Stenotrophomonas maltophilia, no EUCAST MIC breakpoints were available. However, ESBL genes (blaCTXM-1, blaCTX-M-2, blaTEM-1, and blaSHV) and AmpC genes (blaCIT, blaACC, and blaEBC) were found in 38 and 16 isolates, respectively. P. aeruginosa was the only CR species shared by pigs (4/300 pigs; 1.3%) and patients (2/187; 1.1%). P. aeruginosa ST938 carrying blaPAO and blaOXA396 was detected in one pig as well as an 83-year-old patient. Although no direct epidemiological link was demonstrable, SNP calling and cgMLST showed a genetic relationship of the isolates (86 SNPs and 661 allele difference), thus suggesting possible circulation of CR bacteria between swine and humans

Three-dimensional seismic investigations of the Sevastopol mud volcano in correlation to gas/fluid migration pathways and indications for gas hydrate occurrences in the Sorokin Trough (Black Sea)

New 3-D seismic investigations carried out across the Sevastopol mud volcano in the Sorokin Trough present 3-D seismic data of a mud volcano in the Black Sea for the first time. The studies allow us to image the complex three-dimensional morphology of a collapse structured mud volcano and to propose an evolution model. The Sevastopol mud volcano is located above a buried diapiric structure with two ridges and controlled by fluid migration along a deep fault system, which developed during the growth of the diapirs in a compressional tectonic system. Overpressured fluids initiated an explosive eruption generating the collapse depression of the Sevastopol mud volcano. Several cones were formed within the depression by subsequent quiet mud extrusions. Although gas hydrates have been recovered at various mud volcanoes in the Sorokin Trough, no gas hydrates were sampled at the Sevastopol mud volcano. A BSR (bottom-simulating reflector) is missing in the seismic data; however, high-amplitude reflections (bright spots) observed above the diapiric ridge near the mud volcano at a relatively constant depth correspond to the approximate depth of the base of the gas hydrate stability zone (BGHSZ). Thus we suggest that gas hydrates are present locally where gas/fluid flow occurs related to mud volcanism, i.e., above the diapir and close to the feeder channel of the mud volcano. Depth variations of the bright spots of up to 200 ms TWT might be caused by temperature variations produced by variable fluid flow

Kinematic and thermal evolution of the Moroccan rifted continental margin: Doukkala-High Atlas Transect

The Atlantic passive margin of Morocco developed during Mesozoic times in association with the opening of the Central Atlantic and the Alpine Tethys. Extensional basins formed along the future continental margin and in the Atlas rift system. In Alpine times, this system was inverted to form the High and Middle Atlas fold-and-thrust belts. To provide a quantitative kinematic analysis of the evolution of the rifted margin, we present a crustal section crossing the Atlantic margin in the region of the Doukkala Basin, the Meseta and the Atlas system. We construct a post-rift upper crustal section compensating for Tertiary to present vertical movements and horizontal deformations, and we conduct numerical modeling to test quantitative relations between amounts and distribution of thinning and related vertical movements. Rifting along the transect began in the Late Triassic and ended with the appearance of oceanic crust at 175 Ma. Subsidence, possibly related to crustal thinning, continued in the Atlas rift in the Middle Jurassic. The numerical models confirm that the margin experienced a polyphase rifting history. The lithosphere along the transect preserved some strength throughout rifting with the Effective Elastic Thickness corresponding to an isotherm of 450°C. A mid-crustal level of necking of 15 km characterized the pre-rift lithosphere. © 2010 by the American Geophysical Union

Analysis of the major Fe bearing mineral phases in recent lake sediments by EXAFS spectroscopy.

International audienc

The large impact of gold-mining in total-Hg content in sediment of the dam lake of Petit Saut (French Guiana)

International audienceGold-mining is a major source of Hg pollution, especially in the tropical rain forest of South America. This pollution has been shown to strongly increase the local natural background. Does this pollution ex tend far beyond the local areas? Here we present results concerning sediments cored in the large dam lake of Petit-Saut in French Guiana. This dam lake, created in 1994, collects waters from a large catchment area where gold-mining activity has been developing since the last century. and still is. Sediment depth profiles in ^{210}Pb and in total-Hg revealed a high Hg content in the last 4 years. This can only result from anthropogenic activities, which could be due either to propagation of sediments from gold mining sites, or chemical processes acting in recently flooded soils (both processes being related to the dam creation)