Evaluation of binding mode between anticancer drug etoposide and human serum albumin by numerous spectrometric techniques and molecular docking

Etoposide (EPD) is a chemotherapy drug served to treat numerous cancers, and human serum albumin (HSA) functioning as transportation of variant small compounds (exogenous and endogenous). Its binding interaction was studied computationally (molecular docking) and spectrometrically by FT-IR, UV-vis, fluorescence (emission, 3D and synchronous) and CD with pH 7.40 at 292, 300 and 308 K temperatures. The obtained results were imparted that HSA bounded by EPD via static process, ultimately the formation of EPD-HSA complex with strong binding constant (10(5)) through vander Waals and hydrogen bonds. EPD can induce the differing in secondary structure, conformation and microenvironments of HSA upon binding; polarity around Trp increased and its distance to EPD was also analysed. Displacement estimations and molecular docking have demonstrated that EPD notably binds to site III (IB) of HSA. Influence of Co2+, Na+, Fe2+, Zn2+ and Ca2+ ions on EPD-HSA system was examined

A New Feature Selection Method based on Intuitionistic Fuzzy Entropy to Categorize Text Documents

Selection of highly discriminative feature in text document plays a major challenging role in categorization. Feature selection is an important task that involves dimensionality reduction of feature matrix, which in turn enhances the performance of categorization. This article presents a new feature selection method based on Intuitionistic Fuzzy Entropy (IFE) for Text Categorization. Firstly, Intuitionistic Fuzzy C-Means (IFCM) clustering method is employed to compute the intuitionistic membership values. The computed intuitionistic membership values are used to estimate intuitionistic fuzzy entropy via Match degree. Further, features with lower entropy values are selected to categorize the text documents. To find the efficacy of the proposed method, experiments are conducted on three standard benchmark datasets using three classifiers. F-measure is used to assess the performance of the classifiers. The proposed method shows impressive results as compared to other well known feature selection methods. Moreover, Intuitionistic Fuzzy Set (IFS) property addresses the uncertainty limitations of traditional fuzzy set

Synthesis, spectral characterization and some biological studies of transition metal complexes with Schiff base ligand containing N,O and S as donor atoms

Transition metal complexes of Cu(II), Co(II), Ni(II), Zn(II), Cd(II) and Mn(II) have been synthesized with the Schiff base ligand 5-Sub-N-(2-mercaptophenyl)salicylideneimine. Elemental analysis of these complexes suggest that these metal ions forms complexes of type ML(H2O)stoichiometry for Cu(II), Co(II), Ni(II), Zn(II), Cd(II) and Mn(II). The ligand behaves as tridentate and forms coordinate bonds through O, S and N atoms. Magnetic susceptibility, IR, UV – Visible, Mass and ESR spectral studies suggest that Cu(II), Ni(II) complexes posses square planar geometry, whereas Co(II), Zn(II), Cd(II) and Mn(II) complexes posses tetrahedral geometry. The complexes were tested for their antimicrobial activity against the bacterial strains Staphylococcus aureus and Bacillus subtilis.The Schiff base metal complexes evaluated for their antifungal activity against the fungi A. niger and C. oxysporum. The DNA cleavage studies of Schiff base complexes werestudied using Calf – Thymus DNA by agarose gel electrophoresis method

ANTIEPILEPTIC ACTIVITY OF SOME NOVEL SUBSTITUTED FLUORO BENZOTHIAZOLE DERIVATIVES

Objective: The aim of the study is to investigate and to reveal the antiepileptic activity of fluoro substituted benzothiazole derivatives (FBTDs) in experimental induced epilepsy in mice.Methods: The acute oral toxicity was determined as per OECD guideline 423 and minimum effective dose was also determined for pharmacological screening. The antiepileptic activity of FBTDs was studied against maximal electroshock (MES) and strychnine (1 mg/kg, s. c.) induced seizures in mice.Results: In MES test, the duration of tonic hind limb extension was significantly reduced by all FBTDs at a dose of 100 mg/kg except, P-3, and OX-9 whereas in strychnine induced model, the seizure latency was sustained by all FBTDs at a dose of 100 mg/kg except P-4. P-7 and OX-9.Conclusion: The novels synthesized FBTDs shown very good activity and lead a route for further studies in this particular molecule.Â

A Sensitive spectrophotometric determination of ritodrine, pentazocine, isoxsuprine hydrochlorides and amoxicillin in pure and pharmaceutical samples

A simple, accurate and highly sensitive spectrophotometric method for the determination of ritodrine hydrochloride (RTH), pentazocine hydrochloride (PZH), isoxsuprine hydrochloride (ISH) and amoxicillin (AMX) is described. The method is based on the oxidation of the studied drugs by a known excess of chloramine – T (CAT) in hydrochloric acid medium and subsequent determination of the unreacted oxidant by reacting it with iodide in the same acid medium liberates iodine, which subsequently react with starch to form a stable starch-iodine complex. The reacted oxidant corresponds to the drug content. The coloured complex exhibits a maximum absorption at 590 nm. The apparent molar absorptivity values and Sandell’s sensitivity values are in the range 6.96x104 - 1.43x105 L mol–1 cm–1 and 2.45-4.30 ng cm–2, respectively. The method was successfully applied to the studied drugs in their dosage forms. The results are reproducible within ±1% and compare favorably with those of official methods of British Pharmacopoeia and the United States Pharmacopoeia

Electrical Conductivity Studies on Co(II), Cu(II),Ni(II) and Cd(II) Complexes of Azines

1-Phenyl-4-(2'-hydroxyphenyl-1-yl)di-imino azine, {1P-4-(2' HPDA)} 1, 4(2'-hydroxyphenyl-1-yl)di-imino azine, {1, 4-(2' HPDA)} are derived from benzophenone hydrozone with different aldehydes in presence of few drops of concentrated hydrochloric acid in alcoholic medium. Metal(II) complexes have been prepared by salts of CoCl2, CuCl2, NiCl2 and CdCl2 reacting with azine ligands dissolved in alcohol in the molar ratio of (1:2). The prepared complexes were characterized by using various physical methods viz. elemental, molar conductance, magnetic susceptibility, IR, NMR, XRD and UV-Visible. Conductivity of the powder samples were measured by two probe method. Measured electrical conductivities of Co(II), Cu(II), Ni(II) and Cd(II) complexes of azines are reported in this paper. It is found that at room temperature these complexes show insulator behavior. At higher temperature conductivity increases linearly, showing semi conducting behavior

Spectrophotometric Determination of Mycophenolate Mofetil as Its Charge-Transfer Complexes with Two π-Acceptors

Two simple, selective, and rapid spectrophotometric methods are described for the determination of mycophenolate mofetil (MPM) in pure form and in tablets. Both methods are based on charge-transfer complexation reaction of MPM with p-chloranilic acid (p-CA) or 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) in dioxane-acetonitrile medium resulting in coloured product measurable at 520 nm (p-CA) or 580 nm (DDQ). Beer's law is obeyed over the concentration ranges of 40–400 and 12–120 μg mL−1 MPM for p-CA and DDQ, respectively, with correlation coefficients (r) of 0.9995 and 0.9947. The apparent molar absorptivity values are calculated to be 1.06 × 103 and 3.87 × 103 L mol−1 cm−1, respectively, and the corresponding Sandell's sensitivities are 0.4106 and 0.1119 μg cm−1. The limits of detection (LOD) and quantification (LOQ) are also reported for both methods. The described methods were successfully applied to the determination of MPM in tablets. Statistical comparison of the results with those of the reference method showed excellent agreement. No interference was observed from the common excipients present in tablets. Both methods were validated statistically for accuracy and precision. The accuracy and reliability of the methods were further ascertained by recovery studies via standard addition procedure

Synthetic, Spectral and Thermal Studies of Tin(IV) Complexes of 1, 5-Benzodiazepines

Tin(IV) complexes of 7-substituted 6,7-benzo-1,5-dizepines have been synthesized in absolute alcoholic medium. Elemental analysis indicates that the complexes have 1:2 stoichiometry of the type L2SnCl4, TGA data support this conclusion. Molar conductance values in DMF at 10–3 M suggest that, these complexes are non-electrolytes. Infrared spectral data shows the involvement of C=N and NH groups in coordination with the metal ion. X-ray diffraction pattern of few representative complexes indicate that, these are having simple cubic crystal structure. The energy of activation and order of reaction are calculated using TGA data of the complexes. All these information support that Sn(IV) in these complexes exhibits coordination number eight

Antihyperlipidemic activity of Chloroxylon swietenia in triton WR1339 induced hyperlipidemia

Background: Medicinal herbs are beneficial and effective either in the management and prevention of several metabolic disorders, associated with hyperlipidemia, hypertension and insulin resistance which increases the cardio-metabolic risk and demands for the life time therapy. Current allopathic medicines are expensive and reported with several adverse effects and hence, finding of a suitable herbal medicine for hyperlipidemic disorders is very important.Methods: Thirty albino rats weighing 200-230g were randomly divided into 5 groups were rendered hyperlipidemia with a single dose of triton WR 1339. Normal control, positive control, standard, aqueous and ethanolic extract groups were treated with tween-80, tween-80, atorvastatin, aqueous and ethanolic extracts of Chloroxylon swietenia respectively for seven days. At the end of the study, blood was collected for estimation of the lipid profile.Results: Both the aqueous and ethanolic extract groups significantly reduced the TG and VLDL levels.Conclusions: The extracts exhibited remarkable activity on one or either parameter of the lipid profile. It could be due to the presence of alkaloids, steroids, flavonoids, coumarins and phenols in the extracts

N-[3-(4-Fluoro­benz­yl)-2,4-dioxo-1,3-diaza­spiro­[4.5]dec-8-yl]-2-methyl­benzene­sulfonamide

In the title compound, C22H24FN3O4S, the cyclo­hexane ring adopts a chair conformation and the five-membered ring is essentially planar, with a maximum deviation of 0.040 (2) Å. The dihedral angles between the five-membered ring and the tolyl and fluoro­benzene rings are 56.74 (12) and 89.88 (12)°, respectively. The two terminal benzene rings make a dihedral angle of 63.53 (12)°. The crystal structure displays inter­molecular C—H⋯O and N—H⋯O hydrogen bonds. An intra­molecular C—H⋯O hydrogen bond also occurs