Etoposide (EPD) is a chemotherapy drug served to treat numerous cancers, and human serum albumin (HSA) functioning as transportation of variant small compounds (exogenous and endogenous). Its binding interaction was studied computationally (molecular docking) and spectrometrically by FT-IR, UV-vis, fluorescence (emission, 3D and synchronous) and CD with pH 7.40 at 292, 300 and 308 K temperatures. The obtained results were imparted that HSA bounded by EPD via static process, ultimately the formation of EPD-HSA complex with strong binding constant (10(5)) through vander Waals and hydrogen bonds. EPD can induce the differing in secondary structure, conformation and microenvironments of HSA upon binding; polarity around Trp increased and its distance to EPD was also analysed. Displacement estimations and molecular docking have demonstrated that EPD notably binds to site III (IB) of HSA. Influence of Co2+, Na+, Fe2+, Zn2+ and Ca2+ ions on EPD-HSA system was examined
