Agrobacterium mediated transformation of annexin gene in tobacco (Nicotiana tabacum)

The present study involves the development of genetically engineered tobacco plants with annexin gene. The plasmid pUC 119 with the Annexin gene and pGPTV vector were isolated from the Escherichia coli. These plasmids were subjected to restriction digestion with EcoRI and Xbal where the Annexin gene is released from the pUC 119 as a linearised band was eluted from the gel. The recombinant PGPTV plasmid with the annexin gene in Agrobacterium tumefaciens MTCC 431 was mobilized and transferred to plant system through the mobilization helper plasmid pRK2013. The kanamycin resistance gene (NPT II) was used as a selective marker. The calli used for isolating the genomic DNA which was then amplified for confirmation of annexin gene. The nptII gene of 800 bp serves as a selectable marker system in plants and its amplification confirmed the presence of annexin gene in transgenic plants by PCR method

Literacy Development Through the Incorporation of Literature in Language Education for Malaysian Secondary School Students

Literacy is a major concern among educators in the Malaysian education scenario. Various programmes have been identified and implemented in primary and secondary schools to raise the standard of the English language among students. Among them is the implementation of various English language reading programmes. This move has been seen as necessary as part of the Malaysian government\u27s efforts to make all Malaysians proficient in the English language. To this end, the Malaysian Ministry of Education has incorporated literature into the English language subject. Despite the concerted efforts from the various sections of the Ministry of Educations, the effectiveness of these programmes has been unclear. This study looks into the various programmes implemented in the Malaysian primary and secondary schools. Using a questionnaire, the study attempts to identify students\u27 perception on the types of literary genre they enjoy reading. The study also looks into the various activities carried out by English teachers and students\u27 views on the types of activities they enjoy and found useful in the classroom

A Dorsal Hand Vein Recognition-based on Local Gabor Phase Quantization with Whitening Transformation

The hand vein pattern is a biometric feature in which the actual pattern is the shape of the vein network and its characteristics are the vein features. This paper investigates a new approach for dorsal hand vein pattern identification from grey level dorsal hand vein information. In this study Gabor filter quadrature pair is employed to compute locally in a window for every pixel position to extract the phase information. The phases of six frequency coefficients are quantized and it is used to form a descriptor code for the local region. These local descriptors are decorrelated using whitening transformation and a histogram is generated for every pixel which describes the local pattern.  Experiments are evaluated on North China University of Technology  dorsal hand vein image dataset with minimum distance classifier and the results are analyzed for recognition rate, run time and equal error rate. The proposed method gives 100 per cent recognition rate and 1 per cent EER for fusion of both left and right hands.Defence Science Journal, 2014, 64(2), pp. 159-167. DOI: http://dx.doi.org/10.14429/dsj.64.465

Power Consumption and Carbon Emission Equivalent for Virtualized Resources – An Analysis: Virtual Machine and Container Analysis for Greener Data Center

The International Energy Agency (IEA) revealed that the worldwide energy-related carbon dioxide (CO2) situation has hit a historic high of 33.1 Giga tonnes (Gt) of CO2. 85% of the rise in emissions was due to China, India, and the United States. The increase in emissions in India was 4.8%, or 105 Mega tonnes (Mt) of CO2, with the increase in emissions being evenly distributed across the transportation and industrial sectors, according to Beloglazov et al (2011). Environmental contamination brought on by carbon emissions is harmful to the environment. As a result, there is an urgent need for the IT sectors to develop effective and efficient technology to eliminate such carbon emissions. The primary focus is on lowering carbon emissions due to widespread awareness of the issue

Safety and efficacy of intravenous pulse cyclophosphamide therapy in children with steroid-dependent nephrotic syndrome

Background: Cyclophosphamide (Cyp) is a well-known alternative agent to spare the use of steroids and avoid the side effects that result from long-term steroid therapy in children with idiopathic nephrotic syndrome (NS). Use of Cyp typically reduces the risk of relapse in comparison with prednisolone by about 50%. Objective: To study the safety and efficacy of intravenous pulse Cyp (IV Cyp) therapy in children with steroid-dependent NS (SDNS). Materials and Methods: This was a retrospective single-center analysis from a large government hospital in South India. The data were retrieved from the records of children attending the pediatric nephrology clinic between 2005 and 2016. Children with SDNS who received the complete schedule of IV Cyp were included in the study. Children who received other alternate drugs were excluded. Results: Fifty patients with SDNS were treated with IV Cyp, seven patients did not complete the treatment and were excluded from the analysis. 24 (56%) of the 43 patients stayed in remission throughout the course of Cyp treatment and prednisolone could be stopped and were considered as Cyp responsive. The remaining 19 (44%) children had relapsed while on Cyp therapy (Cyp resistant) and required treatment with alternate immunosuppressant drugs. 19 of the 24 Cyp responsive patients were in remission 6 months after stopping Cyp treatment (Cyp sensitive) while the remaining five patients relapsed within 6 months of stopping Cyp treatment (Cyp dependent). 9 children (20.9%) were relapse-free till the time of last follow-up with a median follow-up of 2.1 years. Among 24 children, 13 (30.23%) who responded were infrequently relapsing NS and 2 (4.5%) children were frequently relapsing NS

Phytochemical Investigation of Egyptian Spinach Leaves, a Potential Source for Antileukemic Metabolites: In Vitro and In Silico Study

Spinacia oleracea L., Amaranthaceae, leaves cultivated in Egypt demonstrated a potential antileukemic activity against the chronic myeloid leukemia, K562 cell line. Thus, the aim of this study is to carry out a phytochemical investigation of S. oleracea leaves as well as the isolation of its antileukemic phytoconstituents. Phytochemical investigation of S. oleracea leaves resulted in the isolation of seventeen known compounds. The biological study revealed that compounds hexaprenol, phytol, and 18-[(1-oxohexadecyl) oxy]-9-octadecenoic acid exhibited a remarkable antiproliferative activity against K562 cells in vitro. A mechanistic in silico study showed that hexaprenol, phytol, and 18-[(1-oxohexadecyl) oxy]-9-octadecenoic acid exhibited a strong binding affinity towards topoisomerase (docking score −12.50, −9.19, and −13.29 kcal/mol, respectively), and showed as well a strong binding affinity towards Abl kinase (docking score −11.91, −9.35, and −12.59 kcal/mol, respectively). Molecular dynamics study revealed that 18-[(1-oxohexadecyl) oxy]-9-octadecenoic acid produced stable complexes with both topoisomerase and Abl kinase with RMSD values of 1.81 and 1.85 Å, respectively. As a result of our findings, we recommend more in vivo and preclinical studies to confirm the potential benefit of spinach leaves for chronic myeloid leukemia patients. Graphical Abstract: [Figure not available: see fulltext.