75 research outputs found
Biobank Oversight and Sanctions Under the General Data Protection Regulation
This contribution offers an insight into the function and problems of the oversight and sanctions mechanisms outlined in the General Data Protection Regulation as they relate to the biobanking context. These mechanisms might be considered as meta-mechanisms—mechanisms relating to, but not consisting of, substantive legal principles—functioning in tandem to ensure biobank compliance with data protection principles. Each of the mechanisms outlines, on paper at least, comprehensive and impressive compliance architecture—both expanding on their capacity in relation to Directive 95/46. Accordingly, each mechanism looks likely to have a significant and lasting impact on biobanks and biobanking. Despite this comprehensiveness, however, the mechanisms are not immune from critique. Problems appear regarding the standard of protection provided for research subject rights, regarding the disproportionate impact on legitimate interests tied up with the biobanking process—particularly genomic research interests—and regarding their practical implementability in biobanking
Shortest path queries in rectilinear worlds
Abstract In this paper, a data structure is given for two and higher dimensional shortest path queries. For a set of n axis-parallel rectangles in the plane, or boxes in d-space, and a fixed target, it is possible with this structure to find a shortest rectilinear path avoiding all rectangles or boxes from any point to this target. Alternatively, it is possible to find the length of the path. The metric considered is a generalization of the Ll-metric and the link metric, where the length of a path is its L1-Iength plus some (fixed) constant times the number of turns on the path. The data structure has size 0« n log n )d-l), and a query takes O(logd-l n) time (plus the output size if the path must be reported). As a byproduct, a relatively simple solution to the single shot problem is obtained; the shortest path between two given points can be computed in time O(ndlogn) for d ~ 3, and in time 0(n 2 ) in the plane
Human Apolipoprotein A-I-Derived Amyloid: Its Association with Atherosclerosis
Amyloidoses constitute a group of diseases in which soluble proteins aggregate and deposit extracellularly in tissues. Nonhereditary apolipoprotein A-I (apoA-I) amyloid is characterized by deposits of nonvariant protein in atherosclerotic arteries. Despite being common, little is known about the pathogenesis and significance of apoA-I deposition. In this work we investigated by fluorescence and biochemical approaches the impact of a cellular microenvironment associated with chronic inflammation on the folding and pro-amyloidogenic processing of apoA-I. Results showed that mildly acidic pH promotes misfolding, aggregation, and increased binding of apoA-I to extracellular matrix elements, thus favoring protein deposition as amyloid like-complexes. In addition, activated neutrophils and oxidative/proteolytic cleavage of the protein give rise to pro amyloidogenic products. We conclude that, even though apoA-I is not inherently amyloidogenic, it may produce non hereditary amyloidosis as a consequence of the pro-inflammatory microenvironment associated to atherogenesis
An Abundant Dysfunctional Apolipoprotein A1 in Human Atheroma
Recent studies have indicated that high-density lipoproteins (HDLs) and their major structural protein, apolipoprotein A1 (apoA1), recovered from human atheroma are dysfunctional and are extensively oxidized by myeloperoxidase (MPO). In vitro oxidation of either apoA1 or HDL particles by MPO impairs their cholesterol acceptor function. Here, using phage display affinity maturation, we developed a high-affinity monoclonal antibody that specifically recognizes both apoA1 and HDL that have been modified by the MPO-H2O2-Cl− system. An oxindolyl alanine (2-OH-Trp) moiety at Trp72 of apoA1 is the immunogenic epitope. Mutagenesis studies confirmed a critical role for apoA1 Trp72 in MPO-mediated inhibition of the ATP-binding cassette transporter A1 (ABCA1)-dependent cholesterol acceptor activity of apoA1 in vitro and in vivo. ApoA1 containing a 2-OH-Trp72 group (oxTrp72-apoA1) is in low abundance within the circulation but accounts for 20% of the apoA1 in atherosclerosis-laden arteries. OxTrp72-apoA1 recovered from human atheroma or plasma is lipid poor, virtually devoid of cholesterol acceptor activity and demonstrated both a potent proinflammatory activity on endothelial cells and an impaired HDL biogenesis activity in vivo. Elevated oxTrp72-apoA1 levels in subjects presenting to a cardiology clinic (n = 627) were associated with increased cardiovascular disease risk. Circulating oxTrp72-apoA1 levels may serve as a way to monitor a proatherogenic process in the artery wall
The therapeutic efficacy of intensive medical therapy in ameliorating high-density lipoprotein dysfunction in subjects with type two diabetes
Age and Lineup Type Differences in the Own-Race Bias
The own-race bias (ORB) suggests that recognition for faces of one’s own race is superior to recognition of other-race faces. A popular explanation for the ORB is amount of interracial contact, which may have cohort effects for older and younger adults. We compared White younger and older adults on the ORB utilizing a hybrid facial recognition and full diagnostic lineup (i.e., simultaneous and sequential target absent and target present lineups) paradigm. Both younger and older adults demonstrated an ORB. Signal detection estimates suggest younger adults compared to older adults have better discrimination accuracy for own-race over other-race faces. Interracial contact did not explain recognition for younger adults but was related to a shift in response criterion for older adults
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