Colour-colour diagrams and extragalactic globular cluster ages. Systematic uncertainties using the (V-K)-(V-I) diagram

(abridged) We investigate biases in cluster ages and [Fe/H] estimated from the (V-K)-(V-I) diagram, arising from inconsistent Horizontal Branch morphology, metal mixture, treatment of core convection between observed clusters and the theoretical colour grid employed for age and metallicity determinations. We also study the role played by statistical fluctuations of the observed colours, caused by the low total mass of typical globulars. Horizontal Branch morphology is potentially the largest source of uncertainty. A single-age system harbouring a large fraction of clusters with an HB morphology systematically bluer than the one accounted for in the theoretical colour grid, can simulate a bimodal population with an age difference as large as 8 Gyr. When only the redder clusters are considered, this uncertainty is almost negligible, unless there is an extreme mass loss along the Red Giant Branch phase. The metal mixture affects mainly the redder clusters; the effect of colour fluctuations becomes negligible for the redder clusters, or when the integrated Mv is brighter than -8.5 mag. The treatment of core convection is relevant for ages below ~4 Gyr. The retrieved [Fe/H] distributions are overall only mildly affected. Colour fluctuations and convective core extension have the largest effect. When 1sigma photometric errors reach 0.10 mag, all biases found in our analysis are erased, and bimodal age populations with age differences of up to ~8 Gyr go undetected. The use of both (U-I)-(V-K) and (V-I)-(V-K) diagrams may help disclosing the presence of blue HB stars unaccounted for in the theoretical colour calibration.Comment: 20 pages, including 26 figures. A&A in pres

Diffusive convective overshoot in core He-burning intermediate mass stars. I: the LMC metallicity

We present detailed evolutionary calculations focused on the evolution of intermediate mass stars with 3 Msun < M < 9 Msun of metallicity typical of the Large Magellanic Cloud (LMC), i.e. Z=0.008. We compare carefully the models calculated by adopting a diffusive scheme for chemical mixing, in which nuclear burning and mixing are self-consistently coupled, while the eddy velocities beyond the formal convective core boundary are treated to decay exponentially, and those calculated with the traditional instantaneous mixing approximation. We find that: i) the physical and chemical behaviour of the models during the H-burning phase is independent of the scheme used for the treatment of mixing inside the CNO burning core; ii) the duration of the He-burning phase relative to the MS phase is systematically longer in the diffusive models, due to a slower redistribution of helium to the core from the outer layers; iii) the fraction of time spent in the blue part of the clump, compared to the stay in the red, is larger in the diffusive models. The differences described in points ii) and iii) tend to vanish for M > Msun. In terms of the theoretical interpretation of an open cluster stellar population, the differences introduced by the use of a self-consistent scheme for mixing in the core with adjacent exponential decay are relevant for ages in the range 80 Myr < t < 200 Myr. These results are robust, since they are insensitive to the choice of the free-parameters regulating the extension of the extra-mixing region.Comment: 14 pages, 14 figure, accepted for publication on Astronomy & Astrophysic

Monte Carlo Simulations of Metal-Poor Star Clusters

Metal-poor globular clusters (GCs) can provide a probe of the earliest epoch of star formation in the Universe, being the oldest stellar systems observable. In addition, young and intermediate-age low-metallicity GCs are present in external galaxies. Nevertheless, inferring their evolutionary status by using integrated properties may suffer from large \emph{intrinsic} uncertainty caused by the discrete nature of stars in stellar systems, especially in the case of faint objects. In this paper, we evaluate the \emph{intrinsic} uncertainty (due to statistical effects) affecting the integrated colours and mass--to--light ratios as a function of the cluster integrated visual magnitude ($M_V^{tot}$), which represents a quantity directly measured. Our approach is based on Monte Carlo techniques for randomly generating stars distributed according to the cluster's mass function. Integrated colours and mass--to--light ratios in different photometric bands are checked to be in good agreement with the observational values of low-metallicity Galactic clusters. We present integrated colours and mass--to--light ratios as a function of age for different assumptions on the cluster total $V$ magnitude. We find that the emph{intrinsic} uncertainty cannot be neglected. In particular, in models with $M_V^{tot}=-4$ the broad-band colours show an \emph{intrinsic} uncertainty so high as to prevent precise age evaluation of the cluster. Finally, the present predictions are compared with recent results available in the literature, showing in some cases non-negligible differences.Comment: 18 pages, 12 figures, A&A accepte

Bio-nanotechnology application in wastewater treatment

The nanoparticles have received high interest in the field of medicine and water purification, however, the nanomaterials produced by chemical and physical methods are considered hazardous, expensive, and leave behind harmful substances to the environment. This chapter aimed to focus on green-synthesized nanoparticles and their medical applications. Moreover, the chapter highlighted the applicability of the metallic nanoparticles (MNPs) in the inactivation of microbial cells due to their high surface and small particle size. Modifying nanomaterials produced by green-methods is safe, inexpensive, and easy. Therefore, the control and modification of nanoparticles and their properties were also discussed

Evolution of Sex-Specific Traits through Changes in HOX-Dependent doublesex Expression

Analysis in Drosophila suggests that evolutionary changes in the spatial regulation of the transcription factor doublesex play a key role in the origin, diversification, and loss of sex-specific structures

Time dependent mixing in He-burning cores: the case of NGC1866

We examine the convective core helium burning phase of intermediate mass stars, and investigate the role of coupling of nuclear burning and mixing on the extension and duration of the blue loop phase. We compare the theoretical scenario with the distribution of stars in the colour-magnitude (CM) diagram of the Large Magellanic Cloud (LMC) cluster NGC1866, whose largely populated clump of He-burning stars is equally populated in the blue and red side. We compare the distributions expected by adopting either a diffusive scheme within the instability regions, in which nuclear burning and mixing are self-consistently coupled, or the traditional instantaneous mixing approximation. We analyze with particular care the sensitivity of the results to: a) the e-folding distance with which the velocity of convective eddies decays outside the formal border of the convective regions fixed by the Schwarzschild criterion; b) the convective model adopted to evaluate the temperature gradient; c) the rate of reaction C12+alpha -> O16. Models not including convective overshoot are also commented.Comment: 14 pages,16 figures. Accepted for publication on Astronomy & Astrophysic

Highly Contiguous Assemblies of 101 Drosophilid Genomes

Over 100 years of studies in Drosophila melanogaster and related species in the genus Drosophila have facilitated key discoveries in genetics, genomics, and evolution. While high-quality genome assemblies exist for several species in this group, they only encompass a small fraction of the genus. Recent advances in long-read sequencing allow high-quality genome assemblies for tens or even hundreds of species to be efficiently generated. Here, we utilize Oxford Nanopore sequencing to build an open community resource of genome assemblies for 101 lines of 93 drosophilid species encompassing 14 species groups and 35 sub-groups. The genomes are highly contiguous and complete, with an average contig N50 of 10.5 Mb and greater than 97% BUSCO completeness in 97/101 assemblies. We show that Nanopore-based assemblies are highly accurate in coding regions, particularly with respect to coding insertions and deletions. These assemblies, along with a detailed laboratory protocol and assembly pipelines, are released as a public resource and will serve as a starting point for addressing broad questions of genetics, ecology, and evolution at the scale of hundreds of species