IMPLEMENTACJA KOMPUTEROWEGO PRZETWARZANIA DANYCH BADANIA PROCESÓW RELAKSACYJNYCH Z WYKORZYSTANIEM ROZSZERZONEJ FUNKCJI WYKŁADNICZEJ

The object of research is the development of a specialized measuring information system for the study and control of relaxation processes in materials and technical systems. The purpose of the work is the use of computer technologies to eliminate routine operations associated with the processing of experimental data, increase the speed, accuracy and information content of the process of studying the control of gas sensors. A variant of using computer data processing to automate the processing and primary analysis of experimental data of scientific research and control of the physicochemical parameters of gas-sensitive materials is proposed. The developed computer data processing system provides a practical opportunity to use the measurements of the kinetic characteristics of the gas sensitivity of gas sensors for their experimental research and control and, thus, to achieve higher accuracy and information content. The testing of the developed information-measuring system confirmed its operability and compliance with the requirements for improving the accuracy and speed of the processing process.Przedmiotem badań jest opracowanie specjalistycznego systemu informacji pomiarowej do badania i kontroli procesów relaksacyjnych w materiałach i systemach technicznych. Celem pracy jest wykorzystanie technologii komputerowych do wyeliminowania rutynowych operacji związanych z przetwarzaniem danych eksperymentalnych, zwiększenia szybkości, dokładności i zawartości informacyjnej procesu badania kontroli czujników gazu. Zaproponowano wariant wykorzystania komputerowego przetwarzania danych do automatyzacji przetwarzania i podstawowej analizy danych eksperymentalnych badań naukowych i kontroli parametrów fizykochemicznych materiałów wrażliwych na gaz. Opracowany komputerowy system przetwarzania danych zapewnia praktyczną możliwość wykorzystania pomiarów charakterystyk kinetycznych wrażliwości czujników gazu do ich badań eksperymentalnych i kontroli, a tym samym do osiągnięcia wyższej dokładności i zawartości informacyjnej. Testy opracowanego systemu pomiaru informacji potwierdziły jego funkcjonalność i zgodność z wymaganiami dotyczącymi poprawy dokładności i szybkości procesu przetwarzania

IMPLEMENTATION OF COMPUTER PROCESSING OF RELAXATION PROCESSES INVESTIGATION DATA USING EXTENDED EXPONENTIAL FUNCTION

The object of research is the development of a specialized measuring information system for the study and control of relaxation processes in materials and technical systems. The purpose of the work is the use of computer technologies to eliminate routine operations associated with the processing of experimental data, increase the speed, accuracy and information content of the process of studying the control of gas sensors. A variant of using computer data processing to automate the processing and primary analysis of experimental data of scientific research and control of the physicochemical parameters of gas-sensitive materials is proposed. The developed computer data processing system provides a practical opportunity to use the measurements of the kinetic characteristics of the gas sensitivity of gas sensors for their experimental research and control and, thus, to achieve higher accuracy and information content. The testing of the developed information-measuring system confirmed its operability and compliance with the requirements for improving the accuracy and speed of the processing process

Resistance of Probiotic Bacteria Immobilized on PVP Nanofilaments in Gastrointestinal Juice

The article discusses the stability of various types of immobilized forms of probiotic bacteria in the analogue of gastric juice. Immobilization was carried out in an innovative way: bacterial cells are first fixed to the carrier in the form of PVP nanofilaments, after which the nanofilaments dissolve in the culture medium, and the bacteria, fixing on each other, form biofilms

Physical Activity Associated Proteomics of Skeletal Muscle: Being Physically Active in Daily Life May Protect Skeletal Muscle From Aging

Muscle strength declines with aging and increasing physical activity is the only intervention known to attenuate this decline. In order to adequately investigate both preventive and therapeutic interventions against sarcopenia, a better understanding of the biological changes that are induced by physical activity in skeletal muscle is required. To determine the effect of physical activity on the skeletal muscle proteome, we utilized liquid-chromatography mass spectrometry to obtain quantitative proteomics data on human skeletal muscle biopsies from 60 well-characterized healthy individuals (20–87 years) who reported heterogeneous levels of physical activity (not active, active, moderately active, and highly active). Over 4,000 proteins were quantified, and higher self-reported physical activity was associated with substantial overrepresentation of proteins associated with mitochondria, TCA cycle, structural and contractile muscle, and genome maintenance. Conversely, proteins related to the spliceosome, transcription regulation, immune function, and apoptosis, DNA damage, and senescence were underrepresented with higher self-reported activity. These differences in observed protein expression were related to different levels of physical activity in daily life and not intense competitive exercise. In most instances, differences in protein levels were directly opposite to those reported in the literature observed with aging. These data suggest that being physically active in daily life has strong and biologically detectable beneficial effects on muscle

Restoration of energy homeostasis by SIRT6 extends healthy lifespan

Aging leads to a gradual decline in physical activity and disrupted energy homeostasis. The NAD+-dependent SIRT6 deacylase regulates aging and metabolism through mechanisms that largely remain unknown. Here, we show that SIRT6 overexpression leads to a reduction in frailty and lifespan extension in both male and female B6 mice. A combination of physiological assays, in vivo multi-omics analyses and 13C lactate tracing identified an age-dependent decline in glucose homeostasis and hepatic glucose output in wild type mice. In contrast, aged SIRT6-transgenic mice preserve hepatic glucose output and glucose homeostasis through an improvement in the utilization of two major gluconeogenic precursors, lactate and glycerol. To mediate these changes, mechanistically, SIRT6 increases hepatic gluconeogenic gene expression, de novo NAD+ synthesis, and systemically enhances glycerol release from adipose tissue. These findings show that SIRT6 optimizes energy homeostasis in old age to delay frailty and preserve healthy aging

Crosstalk between Mitochondrial and Sarcoplasmic Reticulum Ca2+ Cycling Modulates Cardiac Pacemaker Cell Automaticity

Mitochondria dynamically buffer cytosolic Ca(2+) in cardiac ventricular cells and this affects the Ca(2+) load of the sarcoplasmic reticulum (SR). In sinoatrial-node cells (SANC) the SR generates periodic local, subsarcolemmal Ca(2+) releases (LCRs) that depend upon the SR load and are involved in SANC automaticity: LCRs activate an inward Na(+)-Ca(2+) exchange current to accelerate the diastolic depolarization, prompting the ensemble of surface membrane ion channels to generate the next action potential (AP).To determine if mitochondrial Ca(2+) (Ca(2+) (m)), cytosolic Ca(2+) (Ca(2+) (c))-SR-Ca(2+) crosstalk occurs in single rabbit SANC, and how this may relate to SANC normal automaticity.Inhibition of mitochondrial Ca(2+) influx into (Ru360) or Ca(2+) efflux from (CGP-37157) decreased [Ca(2+)](m) to 80 ± 8% control or increased [Ca(2+)](m) to 119 ± 7% control, respectively. Concurrent with inhibition of mitochondrial Ca(2+) influx or efflux, the SR Ca(2+) load, and LCR size, duration, amplitude and period (imaged via confocal linescan) significantly increased or decreased, respectively. Changes in total ensemble LCR Ca(2+) signal were highly correlated with the change in the SR Ca(2+) load (r(2) = 0.97). Changes in the spontaneous AP cycle length (Ru360, 111 ± 1% control; CGP-37157, 89 ± 2% control) in response to changes in [Ca(2+)](m) were predicted by concurrent changes in LCR period (r(2) = 0.84).A change in SANC Ca(2+) (m) flux translates into a change in the AP firing rate by effecting changes in Ca(2+) (c) and SR Ca(2+) loading, which affects the characteristics of spontaneous SR Ca(2+) release