11,336 research outputs found

    Application of high-resolution melting for variant scanning in chloroplast gene atpB and atpB-rbcL intergenic spacer region of Crucifer species

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    High-resolution melting (HRM) analysis is a rapid and sensitive method for single nucleotide polymorphism (SNP) analysis. In this study, a novel HRM assay was carried out to detect SNPs in the chloroplast gene atpB which encodes the beta subunit of the ATP synthase and atpB upstream intergenic region. The polymorphisms of the two fragments in intertribal samples from the Cruciferae family and within the species of Brassica napus were detected. Based on this results, we found that HRM were able to determine over 90% of the variants which included single or multiple variants and insertion-deletion polymorphisms (INDELs) and rendered possible genotyping of more closely spaced polymorphisms, although there were several false positives (FPs) and misclassification. Six haplotypes were identified in the intertribal materials. The analysis of 90 B. napus found five variation types and the variations were all located in the intergenic region. In conclusion, HRM analysis is a closed tube assay that is easy to perform and is a more effective approach to identify variant of chloroplast genes. This study will facilitate further functional investigations into the role of chloroplast genes in photosynthesis, phylogeny and molecular evolution.Key words: atpB gene, chloroplast genome, crucifer, high-resolution melt curve analysis, SNP, INDEL

    Ginzburg-Landau-type theory of non-polarized spin superconductivity

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    Since the concept of spin superconductor was proposed, all the related studies concentrate on spin-polarized case. Here, we generalize the study to spin-non-polarized case. The free energy of non-polarized spin superconductor is obtained, and the Ginzburg-Landau-type equations are derived by using the variational method. These Ginzburg-Landau-type equations can be reduced to the spin-polarized case when the spin direction is fixed. Moreover, the expressions of super linear and angular spin currents inside the superconductor are derived. We demonstrate that the electric field induced by super spin current is equal to the one induced by equivalent charge obtained from the second Ginzburg-Landau-type equation, which shows self-consistency of our theory. By applying these Ginzburg-Landau-type equations, the effect of electric field on the superconductor is also studied. These results will help us get a better understanding of the spin superconductor and the related topics such as Bose-Einstein condensate of magnons and spin superfluidity.Comment: 9 pages, 5 figure

    Magnetic control of the pair creation in spatially localized supercritical fields

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    We examine the impact of a perpendicular magnetic field on the creation mechanism of electron-positron pairs in a supercritical static electric field, where both fields are localized along the direction of the electric field. In the case where the spatial extent of the magnetic field exceeds that of the electric field, quantum field theoretical simulations based on the Dirac equation predict a suppression of pair creation even if the electric field is supercritical. Furthermore, an arbitrarily small magnetic field outside the interaction zone can bring the creation process even to a complete halt, if it is sufficiently extended. The mechanism for this magnetically induced complete shutoff can be associated with a reopening of the mass gap and the emergence of electrically dressed Landau levels

    Optimal manufacturing/remanufacturing policies with fixed investment for the underdeveloped remanufacturing system

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    © 2017, Strojarski Facultet. All rights reserved. In an underdeveloped remanufacturing system, poor infrastructure and low technical level impede further development of remanufacturing. To overcome this obstacle, a huge amount of fixed investment is required to improve the remanufacturing system. However, this investment inevitably influences the manufacturer’s manufacturing/remanufacturing decision-making economically. The relationship between fixed investment and recycling ratio was investigated, and the two- and multi-period manufacturing/remanufacturing mixed optimization models were developed. Based on the Karush-Kuhn-Tucker (KKT) conditions, the optimal manufacturing/remanufacturing and fixed investment policies were obtained in closed-form expressions. Moreover, the influences of the fixed investment were analysed. Results show that the optimal policies are significantly influenced by the degree of underdevelopment of the remanufacturing system. When the remanufacturing system is underdeveloped, the manufacturer shows a lack of enthusiasm in remanufacturing, thereby resulting in the decrease of the investment and the recycling ratio. The manufacturer raises the sale price to alleviate the loss caused by fixed investment, but the total manufacturing quantity and profit decrease. In the multi-period case, the manufacturer gradually increases the investment for continuously improving the remanufacturing system to increase the recycling ratio and obtain additional profits from remanufacturing. The proposed models can effectively provide the reference for determining the reasonable manufacturing/remanufacturing and fixed investment policies in the underdeveloped remanufacturing system

    Energy efficiency convergence across countries in the context of China's Belt and Road initiative

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    © 2018 Elsevier Ltd After China launched its “Belt and Road” (BR) initiative, the international community became concerned that it may worsen the environmental performance of the BR countries. Due to a lack of data for empirical testing, this paper addresses this concern through an indirect method and draws the implications of the potential impacts of China's BR initiative. This method empirically examines the effects of trade integration and regional cooperation, two major functions of the BR initiative, on energy efficiency (EE) convergence, a concept that describes the catching up process of EE across countries. A sample of 89 countries was selected to analyse the process of EE convergence from 2000 to 2014. The results indicate that although the gaps in EE among countries around the world become larger after 2010, regional cooperation may lead to a convergence process. It also finds that trade integration has a positive influence on convergence across the countries, especially among middle- and low-income countries. The results suggest that the BR initiative, through its roles in trade integration and regional cooperation, may promote EE convergence among countries. This is a desirable environmental outcome. This research also provides policy implications for both China and the other BR countries

    Antiviral treatment alters the frequency of activating and inhibitory receptor-expressing natural killer cells in chronic Hepatitis B virus infected patients

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    Natural killer (NK) cells play a critical role in innate antiviral immunity, but little is known about the impact of antiviral therapy on the frequency of NK cell subsets. To this aim, we performed this longitudinal study to examine the dynamic changes of the frequency of different subsets of NK cells in CHB patients after initiation of tenofovir or adefovir therapy. We found that NK cell numbers and subset distribution differ between CHB patients and normal subjects; furthermore, the association was found between ALT level and CD158b+ NK cell in HBV patients. In tenofovir group, the frequency of NK cells increased during the treatment accompanied by downregulated expression of NKG2A and KIR2DL3. In adefovir group, NK cell numbers did not differ during the treatment, but also accompanied by downregulated expression of NKG2A and KIR2DL3. Our results demonstrate that treatment with tenofovir leads to viral load reduction, and correlated with NK cell frequencies in peripheral blood of chronic hepatitis B virus infection. In addition, treatments with both tenofovir and adefovir in chronic HBV infected patients induce a decrease of the frequency of inhibitory receptor+ NK cells, which may account for the partial restoration of the function of NK cells in peripheral blood following treatment

    Differential Evolution-based 3D Directional Wireless Sensor Network Deployment Optimization

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    Wireless sensor networks (WSNs) are applied more and more widely in real life. In actual scenarios, 3D directional wireless sensors (DWSs) are constantly employed, thus, research on the real-time deployment optimization problem of 3D directional wireless sensor networks (DWSNs) based on terrain big data has more practical significance. Based on this, we study the deployment optimization problem of DWSNs in the 3D terrain through comprehensive consideration of coverage, lifetime, connectivity of sensor nodes, connectivity of cluster headers and reliability of DWSNs. We propose a modified differential evolution (DE) algorithm by adopting CR-sort and polynomial-based mutation on the basis of the cooperative coevolutionary (CC) framework, and apply it to address deployment problem of 3D DWSNs. In addition, to reduce computation time, we realize implementation of message passing interface (MPI) parallelism. As is revealed by the experimentation results, the modified algorithm proposed in this paper achieves satisfying performance with respect to either optimization results or operation time
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