O DIA DE CAMPO COMO MÉTODO PARA ACRESCER CONHECIMENTO AOS PRODUTORES RURAIS

A extensão rural visa promover o desenvolvimento sustentável no campo, levando conhecimento, tecnologia e resolução de problemas. Objetivou-se promover um dia de campo para estudantes e agricultores com o intuito de melhorar a cadeia produtiva da bovinocultura leiteira, através de palestras voltadas as principais dificuldades encontradas na região. Os temas abordados foram: importância do diagnóstico pós-morte; prevenção e controle da mastite bovina; manejo reprodutivo e manejo de pastagens. A avaliação do evento realizada a partir da aplicação de um questionário mostrou que a maioria dos ouvintes julgou como útil e aplicável os conhecimentos adquiridos

PROJETO PELO ESPECIAL - TERAPIA ASSISTIDA POR ANIMAIS

Terapia Assistida por Animais (TAA) constitui-se na utilização de animais com finalidade terapêutica. Este tratamento destaca resultados positivos na melhora social, emocional ou física de pessoas com danos emocionais e deficiências intelectuais submetidas TAA. Objetivou-se avaliar os impactos causados nos participantes do estudo através da interação dos animais visando a qualidade de vida de idosos institucionalizados. Foram utilizados 3 gatos e 2 cães co-terapeutas no decorrer do ano de 2017. Os animais passaram por exames clínicos e laboratoriais e medidas sanitárias garantindo a interação segura entre homem e animal. A avaliação da eficácia da TAA foi realizada através de questionários aplicados aleatoriamente aos funcionários, idosos aptos a respondê-los e voluntários do local. A TAA atingiu de maneira positiva aos idosos melhorando seu bem-estar, convivência e interação

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form

Análise do polimorfismo de inserção/deleção do gene da enzima conversora da angiotensina e presença de complicações crônicas em pacientes com diabete melito tipo 2

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ATIVIDADES ASSISTIDAS POR ANIMAIS EM AMBIENTE HISPITALAR – PROJETO PELO ESPECIAL

As atividades assistidas por animais (AAA) consistem em visitas nas quais o animal tem papel de co-terapeuta, auxiliando no desenvolvimento e recuperação de crianças, jovens e idosos com necessidades especiais ou enfermidades físicas e psicológicas. Adicionalmente, em ambiente hospitalar, observou-se quea AAA melhorou o humor dos pacientes internados, em 20 a 50% dos casos. Animais podem ser utilizadosna AAA com crianças, adolescentes, adultos e idosos em estado agudo ou crônico, na comunicação e nareabilitação. Alguns dos benefícios para aqueles com quem os animais interagem (pacientes, familiares efuncionários das instituições) podem incluir pressão arterial mais baixa, estresse ou ansiedade reduzidos e aliberação de endorfinas, o que faz com que as pessoas se sintam bem. Os animais também podem servircomo uma "ponte" entre o contato humano. Se alguém está deprimido ou tímido, quando colocado em umnovo ambiente, ele pode se retirar. No entanto, quando apresentado a um animal, esse pode ser o estímulopara envolvê-lo nesse novo ambiente. Apesar de a AAA ter como foco melhorar a qualidade de vida depessoas em situação desfavorável, a terapia também impacta na equipe que a conduz, pois, o contato doacadêmico ou professor com realidades diferentes das suas proporciona crescimento pessoal enquantoindivíduo, valorização à vida e à família, bem como a autorrealização. O objetivo do presente projeto deextensão foi a continuidade de atividades já desenvolvidas no Recanto do Idoso em 2015 e 2017, apoiadaspelo edital 74/2015 e 31/2016, bem como na Associação dos Pais e Amigos dos Excepcionais – APAE,desenvolvidas em 2016, edital 10/2016. Assim, objetivou-se abranger não só instituições já visitadas, comotambém, inserir a AAA no ambiente hospitalar, a exemplo de grandes centros urbanos. Foram atendidospacientes internados em hospital na cidade de Lindóia do Sul - SC. Para as AAA, três gatos e três cãestreinados e familiarizados com as atividades foram utilizados em visitas semanais de 50 minutos de duraçãomédia. Foram proporcionadas interações entre os animais e pessoas; os animais foram acariciados,escovados, conduzidos na guia, colocados no colo. Os cães recebiam comandos, como “senta”, “pata”, demodo que o paciente tinha a oportunidade de interagir com o animal, sentindo-se importante e especial noprocesso. No hospital visitado, havia dois locais para as AAA - os quartos na internação, nos quais estavamos pacientes acamados e outro, no qual um grupo de pacientes convivia em grupo. As AAA foram ajustadasaos locais, de forma que nos quartos o período de contato era menor, cerca de 5 minutos por paciente epriorizava-se a conversa e o acariciar do animal. No ambiente de convívio em grupo era possível realizaratividades mais diversificadas, e o tempo de contato com cada paciente era maior. Foram atendidossobretudo adultos e idosos com diferentes enfermidades, a maioria em situação de cuidados prolongados;observou-se que muitos pacientes adultos tinham ansiedade, depressão, síndrome do pânico e doenças deordem física acometiam mais pacientes idosos. O hospital atendido é um dos poucos autorizados (total 8) aatuar como unidade de cuidados prolongados no estado de SC, assim as AAA se mostraram de extremaimportância como forma de modificar a semana do paciente, alterando sua rotina de forma positiva

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form.This work was supported by Goethe University (Corona funds), the DFG-funded CRC: “Molecular Principles of RNA-Based Regulation,” DFG infrastructure funds (project numbers: 277478796, 277479031, 392682309, 452632086, 70653611), the state of Hesse (BMRZ), the Fondazione CR Firenze (CERM), and the IWB-EFRE-program 20007375. This project has received funding from the European Union’s Horizon 2020 research and innovation program under Grant Agreement No. 871037. AS is supported by DFG Grant SCHL 2062/2-1 and by the JQYA at Goethe through project number 2019/AS01. Work in the lab of KV was supported by a CoRE grant from the University of New Hampshire. The FLI is a member of the Leibniz Association (WGL) and financially supported by the Federal Government of Germany and the State of Thuringia. Work in the lab of RM was supported by NIH (2R01EY021514) and NSF (DMR-2002837). BN-B was supported by theNSF GRFP.MCwas supported byNIH (R25 GM055246 MBRS IMSD), and MS-P was supported by the HHMI Gilliam Fellowship. Work in the labs of KJ and KT was supported by Latvian Council of Science Grant No. VPP-COVID 2020/1-0014. Work in the UPAT’s lab was supported by the INSPIRED (MIS 5002550) project, which is implemented under the Action “Reinforcement of the Research and Innovation Infrastructure,” funded by the Operational Program “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014–2020) and cofinanced by Greece and the EU (European Regional Development Fund) and the FP7 REGPOT CT-2011- 285950–“SEE-DRUG” project (purchase of UPAT’s 700MHz NMR equipment). Work in the CM-G lab was supported by the Helmholtz society. Work in the lab of ABö was supported by the CNRS, the French National Research Agency (ANR, NMRSCoV2- ORF8), the Fondation de la Recherche Médicale (FRM, NMR-SCoV2-ORF8), and the IR-RMN-THC Fr3050 CNRS. Work in the lab of BM was supported by the Swiss National Science Foundation (Grant number 200020_188711), the Günthard Stiftung für Physikalische Chemie, and the ETH Zurich. Work in the labs of ABö and BM was supported by a common grant from SNF (grant 31CA30_196256). This work was supported by the ETHZurich, the grant ETH40 18 1, and the grant Krebsliga KFS 4903 08 2019. Work in the lab of the IBS Grenoble was supported by the Agence Nationale de Recherche (France) RA-COVID SARS2NUCLEOPROTEIN and European Research Council Advanced Grant DynamicAssemblies. Work in the CA lab was supported by Patto per il Sud della Regione Siciliana–CheMISt grant (CUP G77B17000110001). Part of this work used the platforms of the Grenoble Instruct-ERIC center (ISBG; UMS 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-05-02) and GRAL, financed within the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE- 0003). Work at the UW-Madison was supported by grant numbers NSF MCB2031269 and NIH/NIAID AI123498. MM is a Ramón y Cajal Fellow of the Spanish AEI-Ministry of Science and Innovation (RYC2019-026574-I), and a “La Caixa” Foundation (ID 100010434) Junior Leader Fellow (LCR/BQ/PR19/11700003). Funded by project COV20/00764 fromthe Carlos III Institute of Health and the SpanishMinistry of Science and Innovation to MMand DVL. VDJ was supported by the Boehringer Ingelheim Fonds. Part of this work used the resources of the Italian Center of Instruct-ERIC at the CERM/ CIRMMP infrastructure, supported by the Italian Ministry for University and Research (FOE funding). CF was supported by the Stiftung Polytechnische Gesellschaft. Work in the lab of JH was supported by NSF (RAPID 2030601) and NIH (R01GM123249).Peer reviewe

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form

Núcleos de Ensino da Unesp: artigos 2008

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Reproducibility of fluorescent expression from engineered biological constructs in E. coli

We present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions around the world measured fluorescence from three engineered constitutive constructs in E. coli. Few participants were able to measure absolute fluorescence, so data was analyzed in terms of ratios. Precision was strongly related to fluorescent strength, ranging from 1.54-fold standard deviation for the ratio between strong promoters to 5.75-fold for the ratio between the strongest and weakest promoter, and while host strain did not affect expression ratios, choice of instrument did. This result shows that high quantitative precision and reproducibility of results is possible, while at the same time indicating areas needing improved laboratory practices.Peer reviewe

Brazilian Flora 2020: Leveraging the power of a collaborative scientific network

International audienceThe shortage of reliable primary taxonomic data limits the description of biological taxa and the understanding of biodiversity patterns and processes, complicating biogeographical, ecological, and evolutionary studies. This deficit creates a significant taxonomic impediment to biodiversity research and conservation planning. The taxonomic impediment and the biodiversity crisis are widely recognized, highlighting the urgent need for reliable taxonomic data. Over the past decade, numerous countries worldwide have devoted considerable effort to Target 1 of the Global Strategy for Plant Conservation (GSPC), which called for the preparation of a working list of all known plant species by 2010 and an online world Flora by 2020. Brazil is a megadiverse country, home to more of the world's known plant species than any other country. Despite that, Flora Brasiliensis, concluded in 1906, was the last comprehensive treatment of the Brazilian flora. The lack of accurate estimates of the number of species of algae, fungi, and plants occurring in Brazil contributes to the prevailing taxonomic impediment and delays progress towards the GSPC targets. Over the past 12 years, a legion of taxonomists motivated to meet Target 1 of the GSPC, worked together to gather and integrate knowledge on the algal, plant, and fungal diversity of Brazil. Overall, a team of about 980 taxonomists joined efforts in a highly collaborative project that used cybertaxonomy to prepare an updated Flora of Brazil, showing the power of scientific collaboration to reach ambitious goals. This paper presents an overview of the Brazilian Flora 2020 and provides taxonomic and spatial updates on the algae, fungi, and plants found in one of the world's most biodiverse countries. We further identify collection gaps and summarize future goals that extend beyond 2020. Our results show that Brazil is home to 46,975 native species of algae, fungi, and plants, of which 19,669 are endemic to the country. The data compiled to date suggests that the Atlantic Rainforest might be the most diverse Brazilian domain for all plant groups except gymnosperms, which are most diverse in the Amazon. However, scientific knowledge of Brazilian diversity is still unequally distributed, with the Atlantic Rainforest and the Cerrado being the most intensively sampled and studied biomes in the country. In times of “scientific reductionism”, with botanical and mycological sciences suffering pervasive depreciation in recent decades, the first online Flora of Brazil 2020 significantly enhanced the quality and quantity of taxonomic data available for algae, fungi, and plants from Brazil. This project also made all the information freely available online, providing a firm foundation for future research and for the management, conservation, and sustainable use of the Brazilian funga and flora