Marginal integrity of restorations produced with a model composite based on polyhedral oligomeric silsesquioxane (POSS)

Marginal integrity is one of the most crucial aspects involved in the clinical longevity of resin composite restorations.Objective To analyze the marginal integrity of restorations produced with a model composite based on polyhedral oligomeric silsesquioxane (POSS).Material and Methods A base composite (B) was produced with an organic matrix with UDMA/TEGDMA and 70 wt.% of barium borosilicate glass particles. To produce the model composite, 25 wt.% of UDMA were replaced by POSS (P25). The composites P90 and TPH3 (TP3) were used as positive and negative controls, respectively. Marginal integrity (%MI) was analyzed in bonded class I cavities. The volumetric polymerization shrinkage (%VS) and the polymerization shrinkage stress (Pss - MPa) were also evaluated.Results The values for %MI were as follows: P90 (100%) = TP3 (98.3%) = B (96.9%) >; P25 (93.2%), (

Assessment of the quality of professional life of nurses.

Context: The verification of quality of life at work (QLW) indices can provide information on factors that directly interfere with personal and collective satisfaction and motivation, with reflections on the excellence of the structure and service. Objective: To evaluate the quality of life at work of active nurses. Material and methods: descriptive and correlational, cross-sectional study with a quantitative approach, carried out with 104 nurses in the service of a tertiary public hospital, in the municipality of Aracaju/SE from August 2018 to July 2019. Results: Factors such as working conditions, safety and remuneration are degrading factors in the quality of life at work and these also have direct repercussions on health and the quality of nursing care provided. Conclusion: Low levels of quality of life at work have direct negative repercussions on nurses' health. It is suggested that further studies evaluate the measures taken by hospital institutions to investigate the effectiveness of quality of life at work in nursing.  Context: The verification of quality of life at work (QLW) indices can provide information on factors that directly interfere with personal and collective satisfaction and motivation, with reflections on the excellence of the structure and service. Objective: To evaluate the quality of life at work of active nurses. Material and methods: descriptive and correlational, cross-sectional study with a quantitative approach, carried out with 104 nurses in the service of a tertiary public hospital, in the municipality of Aracaju/SE from August 2018 to July 2019. Results: Factors such as working conditions, safety and remuneration are degrading factors in the quality of life at work and these also have direct repercussions on health and the quality of nursing care provided. Conclusion: Low levels of quality of life at work have direct negative repercussions on nurses' health. It is suggested that further studies evaluate the measures taken by hospital institutions to investigate the effectiveness of quality of life at work in nursing

Complexity-entropy causality plane: a useful approach for distinguishing songs

Nowadays we are often faced with huge databases resulting from the rapid growth of data storage technologies. This is particularly true when dealing with music databases. In this context, it is essential to have techniques and tools able to discriminate properties from these massive sets. In this work, we report on a statistical analysis of more than ten thousand songs aiming to obtain a complexity hierarchy. Our approach is based on the estimation of the permutation entropy combined with an intensive complexity measure, building up the complexity-entropy causality plane. The results obtained indicate that this representation space is very promising to discriminate songs as well as to allow a relative quantitative comparison among songs. Additionally, we believe that the here-reported method may be applied in practical situations since it is simple, robust and has a fast numerical implementation.Comment: Accepted for publication in Physica

Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/Clavanins is a class of peptides (23aa) histidine-rich, free of post-translational modifications. Clavanins have been studied largely for their ability to disrupt bacterial membranes. In the present study, the interaction of clavanin A with membranes was assessed by dynamic light scattering, zeta potential and permeabilization assays. We observed through those assays that clavanin A lysis bacterial cells at concentrations corresponding to its MIC. Further, the structure and function of clavanin A was investigated. To better understand how clavanin interacted with bacteria, its NMR structure was elucidated. The solution state NMR structure of clavanin A in the presence of TFE-d3 indicated an α-helical conformation. Secondary structures, based on circular dichroism measurements in anionic sodium dodecyl sulfate (SDS) and TFE (2,2,2-trifluorethanol), in silico lipid-peptide docking and molecular simulations with lipids DPPC and DOPC revealed that clavanin A can adopt a variety of folds, possibly influencing its different functions. Microcalorimetry assays revealed that clavanin A was capable of discriminating between different lipids. Finally, clavanin A was found to eradicate bacterial biofilms representing a previously unrecognized function.We would like to thank CNPq, CAPES (Ciências sem Fronteiras), FAPDF and FUNDECT. D.G. acknowledges Fundação para a Ciência e a Tecnologia - Ministério da Educação e Ciência (FCT-MEC, Portugal) for fellowship SFRH/BPD/73500/2010 and A.S.V. for funding within the FCT Investigator Programme (IF/00803/2012).info:eu-repo/semantics/publishedVersio

Simple to be Useful: Ecosystem Base for Coastal Management

Os processos de Gerenciamento Costeiro (GC) nas últimas décadas vêm evoluindo, apresentando diferentes métodos de gestão, sendo que uma nova fronteira se encontra na Gestão com Base Ecossistêmica (GBE). No entanto, para colocar em prática a GBE, a qual leva em consideração as funções, os processos e os serviços ecossistêmicos dos ambientes costeiros e marinhos, entendendo-os como um conjunto de ecossistemas compostos por elementos ecológicos (naturais), econômicos e sociais, se faz necessária a base de informação ecossistêmica. O presente trabalho propõe, apresentando resultados aplicados, um roteiro metodológico de seis etapas: 1. Identificar os ecossistemas como “Unidades de Gestão”; 2. Mapear, modelar e simular os ecossistemas; 3. Identificar e classificar os serviços ecossistêmicos; 4. Definir os valores e a qualidade dos serviços; 5. Identificar os espaços de gestão; e 6. Integrar com políticas e demais instrumentos de gestão e legais. As aplicações práticas apresentadas vão desde trabalhos acadêmicos de identificação e caracterização de ambientes costeiros e marinhos a aplicações nos processos de gestão ambiental portuários, passando também pelo desenvolvimento de Zoneamentos Ecológico-Econômicos (ZEEs) em nível regional, por exemplo. Na apreciação integral do conjunto de exemplos e iniciativas para todas as etapas do modelo, depreende-se que a sua aplicação é ampla, variada e consideravelmente simples. Da mesma forma, a multiplicidade de possíveis aplicações do modelo em ações voltadas ao suporte de uma GBE sugere que seu uso pode crescer e buscar iniciativas inovadoras. A expectativa dos autores é de que tal ferramenta possa, de fato, delinear e estimular pesquisas e aplicações com base ecossistêmica na busca da sustentabilidade da costa e do bem-estar de seus atores sociais.In the last decades Coastal Management (CM) processes have been evolving, presenting different management methods, and the new frontier is at the so-called Ecosystem Based Management (EBM). However, to put into practice EBM, which takes into account ecosystem functions, processes and services of coastal and marine environments, understanding them as a set of ecosystems composed of ecological (natural), economic and social elements, it is necessary an ecosystem-based information. The present work proposes, presenting practical results, a methodological path of six stages: 1. Identification of ecosystems as "Management Units"; 2. Mapping, modeling and simulating ecosystems and their connections; 3. Identification and classification of ecosystem services; 4. Definition of values and quality of services; 5. Identification of related management procedures; and 6. Integration with policies and other management and legal tools. The concrete applications range from academic studies of identification and characterization of coastal and marine environments, to port’s environmental management processes, and the development of ecological-economic zoning at regional level, for example. In the full appreciation of the set of examples and initiatives for all stages of the model, it can be concluded that its application is wide, varied and considerably simple. Likewise, the multiplicity of possible applications of the model in practical actions aimed to support of an EBM, suggests that its use can grow and pursue innovative initiatives. The authors' expectation is that such a tool may, in fact, delineate and stimulate research and applications based on ecosystems in the quest for the sustainability of the coast and the well-being of its social actors

Genome of Herbaspirillum seropedicae Strain SmR1, a Specialized Diazotrophic Endophyte of Tropical Grasses

The molecular mechanisms of plant recognition, colonization, and nutrient exchange between diazotrophic endophytes and plants are scarcely known. Herbaspirillum seropedicae is an endophytic bacterium capable of colonizing intercellular spaces of grasses such as rice and sugar cane. The genome of H. seropedicae strain SmR1 was sequenced and annotated by The Paraná State Genome Programme—GENOPAR. The genome is composed of a circular chromosome of 5,513,887 bp and contains a total of 4,804 genes. The genome sequence revealed that H. seropedicae is a highly versatile microorganism with capacity to metabolize a wide range of carbon and nitrogen sources and with possession of four distinct terminal oxidases. The genome contains a multitude of protein secretion systems, including type I, type II, type III, type V, and type VI secretion systems, and type IV pili, suggesting a high potential to interact with host plants. H. seropedicae is able to synthesize indole acetic acid as reflected by the four IAA biosynthetic pathways present. A gene coding for ACC deaminase, which may be involved in modulating the associated plant ethylene-signaling pathway, is also present. Genes for hemagglutinins/hemolysins/adhesins were found and may play a role in plant cell surface adhesion. These features may endow H. seropedicae with the ability to establish an endophytic life-style in a large number of plant species