Dwarf spheroidal satellites of M31: I. Variable stars and stellar populations in Andromeda XIX

We present B,V time-series photometry of Andromeda XIX (And XIX), the most extended (half-light radius of 6.2') of Andromeda's dwarf spheroidal companions, that we observed with the Large Binocular Cameras at the Large Binocular Telescope. We surveyed a 23'x 23' area centered on And XIX and present the deepest color magnitude diagram (CMD) ever obtained for this galaxy, reaching, at V~26.3 mag, about one magnitude below the horizontal branch (HB). The CMD shows a prominent and slightly widened red giant branch, along with a predominantly red HB, which, however, extends to the blue to significantly populate the classical instability strip. We have identified 39 pulsating variable stars, of which 31 are of RR Lyrae type and 8 are Anomalous Cepheids (ACs). Twelve of the RR Lyrae variables and 3 of the ACs are located within And XIX's half light radius. The average period of the fundamental mode RR Lyrae stars ( = 0.62 d, \sigma= 0.03 d) and the period-amplitude diagram qualify And XIX as an Oosterhoff-Intermediate system. From the average luminosity of the RR Lyrae stars ( = 25.34 mag, \sigma= 0.10 mag) we determine a distance modulus of (m-M)$_0$=$24.52\pm0.23$ mag in a scale where the distance to the Large Magellanic Cloud (LMC) is $18.5\pm0.1$ mag. The ACs follow a well defined Period-Wesenheit (PW) relation that appears to be in very good agreement with the PW relationship defined by the ACs in the LMC.Comment: accepted for publication in Ap

VARIABLE STARS AND STELLAR POPULATIONS IN ANDROMEDA XXV. III. A CENTRAL CLUSTER OR THE GALAXY NUCLEUS?

We present B and V time series photometry of Andromeda XXV, the third galaxy in our program on the Andromeda’s satellites, which we have imaged with the Large Binocular Cameras of the Large Binocular Telescope. The field of Andromeda XXV is found to contain 62 variable stars, for which we present light curves and characteristics of the light variation (period, amplitudes, variability type, mean magnitudes, etc.). The sample includes 57 RR Lyrae variables (46 fundamental-mode—RRab, and 11 first-overtone—RRc, pulsators), 3 anomalous Cepheids, 1 eclipsing binary system, and 1 unclassified variable. The average period of the RRab stars ( =0.60 σ = 0.04 days) and the period–amplitude diagram place Andromeda XXV in the class of the Oosterhoff-Intermediate objects. From the average luminosity of the RR Lyrae stars we derive for the galaxy a distance modulus of (m–M)0 = 24.63 ± 0.17 mag. The color–magnitude diagram reveals the presence in Andromeda XXV of a single, metal-poor ([Fe/H] = ‑1.8 dex) stellar population as old as ∼10–12 Gyr, traced by a conspicuous red giant branch and the large population of RR Lyrae stars. We discovered a spherically shaped high density of stars near the galaxy center. This structure appears to be at a distance consistent with Andromeda XXV and we suggest it could either be a star cluster or the nucleus of Andromeda XXV. We provide a summary and compare the number and characteristics of the pulsating stars in the M31 satellites analyzed so far for variability. Based on data collected with the Large Binocular Cameras at the Large Binocular Telescope

Variable Stars and Stellar Populations in Andromeda XXVII. IV. An Off-centered, Disrupted Galaxy

We present B and V time-series photometry of the M31 satellite galaxy Andromeda XXVII (And XXVII) that we observed with the Large Binocular Cameras of the Large Binocular Telescope. In the field of And XXVII we have discovered a total of 90 variables: 89 RR Lyrae stars and 1 Anomalous Cepheid. The average period of the fundamental mode RR Lyrae stars (RRab) < {P}{ab}> =0.59 {days} (σ = 0.05 day) and the period–amplitude diagram place And XXVII in the class of Oosterhoff I/Intermediate objects. Combining information from the color–magnitude diagram (CMD) and the variable stars, we find evidence for a single old and metal-poor stellar population with [Fe/H] ∼ ‑1.8 dex and t ∼ 13 Gyr in And XXVII. The spatial distributions of RR Lyrae and red giant branch (RGB) stars give clear indication that And XXVII is a completely disrupted system. This is also supported by the spread observed along the line of sight in the distance to the RR Lyrae stars. The highest concentration of RGB and RR Lyrae stars is found in a circular area of 4 arcmin in radius, centered about 0.°2 in the southeast direction from Richardson et al.’s center coordinates of And XXVII. The CMD of this region is well-defined, with a prominent RGB and 15 RR Lyrae stars (out of the 18 found in the region) tracing a very tight horizontal branch at < V({RR})> =25.24 {mag} σ = 0.06 mag (average over 15 stars). We show that And XXVII is a strong candidate building block of the M31 halo. Based on data collected with the Large Binocular Cameras at the Large Binocular Telescope, PI: G. Clementini

BRD4 inhibition enhances azacitidine efficacy in acute myeloid leukemia and myelodysplastic syndromes

Myelodysplastic syndromes (MDS) are clonal hematopoietic stem cell-based disorders characterized by ineffective hematopoiesis, increased genomic instability and a tendency to progress toward acute myeloid leukemia (AML). MDS and AML cells present genetic and epigenetic abnormalities and, due to the heterogeneity of thesemolecular alterations, the current treatment options remain unsatisfactory. Hypomethylating agents (HMA), especially azacitidine, are the mainstay of treatment for high-risk MDS patients and HMA are used in treating elderly AML. The aim of this study was to investigate the potential role of the epigenetic reader bromodomain-containing protein-4 (BRD4) in MDS and AML patients. We identified the upregulation of the short variant BRD4 in MDS and AML patients, which was associated with a worse outcome of MDS. Furthermore, the inhibition of BRD4 in vitro with JQ1 or shRNA induced leukemia cell apoptosis, especially when combined to azacitidine, and triggered the activation of the DNA damage response pathway. JQ1 and AZD6738 (a specific ATR inhibitor) also synergized to induce apoptosis in leukemia cells. Our results indicate that the BRD4-dependent transcriptional program is a defective pathway in MDS and AML pathogenesis and its inhibition induces apoptosis of leukemia cells, which is enhanced in combination with HMA or an ATR inhibitor9CNPQ - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPESP – Fundação de Amparo à Pesquisa Do Estado De São Paulonão tem2011/51959-

Variability and star formation in Leo T, the lowest luminosity star-forming galaxy known today

We present results from the first combined study of variable stars and star formation history (SFH) of the Milky Way (MW) "ultra-faint" dwarf (UFD) galaxy Leo T, based on F606W and F814W multi-epoch archive observations obtained with the Wide Field Planetary Camera 2 on board the Hubble Space Telescope. We have detected 14 variable stars in the galaxy. They include one fundamental-mode RR Lyrae star and 10 Anomalous Cepheids with periods shorter than 1 day, thus suggesting the occurrence of multiple star formation episodes in this UFD, of which one about 10 Gyr ago produced the RR Lyrae star. A new estimate of the distance to Leo T of 409 $^{+29}_{-27}$ kpc (distance modulus of 23.06 $\pm$ 0.15 mag) was derived from the galaxy's RR Lyrae star. Our V, V-I color-magnitude diagram of Leo T reaches V~29 mag and shows features typical of a galaxy in transition between dwarf irregular and dwarf spheroidal types. A quantitative analysis of the star formation history, based on the comparison of the observed V,V-I CMD with the expected distribution of stars for different evolutionary scenarios, confirms that Leo T has a complex star formation history dominated by two enhanced periods about 1.5 and 9 Gyr ago, respectively. The distribution of stars and gas shows that the galaxy has a fairly asymmetric structure.Comment: 31 pages, 14 figures, accepted for publication in Ap

ISOLAMENTO DE DERMATÓFITOS DO PELO DE CÃES E GATOS PERTENCENTES A PROPRIETÁRIOS COM DIAGNÓSTICO DE DERMATOFITOSE

Este estudo avaliou o potencial de cães e gatos em disseminar fungos dermatófitos, o perfil epidemiológico mais suscetível e as principais síndromes clínicas associados à dermatofitose humana. Foram coletados e cultivados pêlos e escamas de 21 gatos e 33 cães domiciliados com 25 proprietários com diagnóstico de dermatofitose. Tanto crianças como adultos adquiriram a dermatofitose a partir do contato com cães e gatos. A maioria das crianças apresentava Tinea capitis enquanto os adultos apresentavam Tinea corporis. Dos animais avaliados, 67% (36/54) foram positivos para fungos dermatófitos, sendo 95% (20/21) dos gatos e 49% (16/33) dos cães positivos. Dos gatos analisados, tanto assintomáticos como sintomáticos podem ter transmitido a doença. Quanto aos cães, os assintomáticos foram provavelmente os principais veiculadores da dermatofitose aos seus proprietários. Das amostras positivas em gatos, e em 94,4% das positivas em cães, o dermatófito isolado foi o Microsporum canis. 

Hide and seek between Andromeda's halo, disk, and giant stream

Photometry in B, V (down to V ~ 26 mag) is presented for two 23' x 23' fields of the Andromeda galaxy (M31) that were observed with the blue channel camera of the Large Binocular Telescope during the Science Demonstration Time. Each field covers an area of about 5.1kpc x 5.1kpc at the distance of M31 ((m-M)o ~ 24.4 mag), sampling, respectively, a northeast region close to the M31 giant stream (field S2), and an eastern portion of the halo in the direction of the galaxy minor axis (field H1). The stream field spans a region that includes Andromeda's disk and the giant stream, and this is reflected in the complexity of the color magnitude diagram of the field. One corner of the halo field also includes a portion of the giant stream. Even though these demonstration time data were obtained under non-optimal observing conditions the B photometry, acquired in time-series mode, allowed us to identify 274 variable stars (among which 96 are bona fide and 31 are candidate RR Lyrae stars, 71 are Cepheids, and 16 are binary systems) by applying the image subtraction technique to selected portions of the observed fields. Differential flux light curves were obtained for the vast majority of these variables. Our sample includes mainly pulsating stars which populate the instability strip from the Classical Cepheids down to the RR Lyrae stars, thus tracing the different stellar generations in these regions of M31 down to the horizontal branch of the oldest (t ~ 10 Gyr) component.Comment: 59 pages, 26 figures, 12 tables, ApJ in pres

BRD4 Inhibition Enhances Azacitidine Efficacy in Acute Myeloid Leukemia and Myelodysplastic Syndromes

Myelodysplastic syndromes (MDS) are clonal hematopoietic stem cell-based disorders characterized by ineffective hematopoiesis, increased genomic instability and a tendency to progress toward acute myeloid leukemia (AML). MDS and AML cells present genetic and epigenetic abnormalities and, due to the heterogeneity of these molecular alterations, the current treatment options remain unsatisfactory. Hypomethylating agents (HMA), especially azacitidine, are the mainstay of treatment for high-risk MDS patients and HMA are used in treating elderly AML. The aim of this study was to investigate the potential role of the epigenetic reader bromodomain-containing protein-4 (BRD4) in MDS and AML patients. We identified the upregulation of the short variant BRD4 in MDS and AML patients, which was associated with a worse outcome of MDS. Furthermore, the inhibition of BRD4 in vitro with JQ1 or shRNA induced leukemia cell apoptosis, especially when combined to azacitidine, and triggered the activation of the DNA damage response pathway. JQ1 and AZD6738 (a specific ATR inhibitor) also synergized to induce apoptosis in leukemia cells. Our results indicate that the BRD4-dependent transcriptional program is a defective pathway in MDS and AML pathogenesis and its inhibition induces apoptosis of leukemia cells, which is enhanced in combination with HMA or an ATR inhibitor