Analysis of a marine phototrophic biofilm by confocal laser scanning microscopy using the new image quantification software PHLIP

BACKGROUND: Confocal laser scanning microscopy (CLSM) is the method of choice to study interfacial biofilms and acquires time-resolved three-dimensional data of the biofilm structure. CLSM can be used in a multi-channel modus where the different channels map individual biofilm components. This communication presents a novel image quantification tool, PHLIP, for the quantitative analysis of large amounts of multichannel CLSM data in an automated way. PHLIP can be freely downloaded from RESULTS: PHLIP is an open source public license Matlab toolbox that includes functions for CLSM imaging data handling and ten image analysis operations describing various aspects of biofilm morphology. The use of PHLIP is here demonstrated by a study of the development of a natural marine phototrophic biofilm. It is shown how the examination of the individual biofilm components using the multi-channel capability of PHLIP allowed the description of the dynamic spatial and temporal separation of diatoms, bacteria and organic and inorganic matter during the shift from a bacteria-dominated to a diatom-dominated phototrophic biofilm. Reflection images and weight measurements complementing the PHLIP analyses suggest that a large part of the biofilm mass consisted of inorganic mineral material. CONCLUSION: The presented case study reveals new insight into the temporal development of a phototrophic biofilm where multi-channel imaging allowed to parallel monitor the dynamics of the individual biofilm components over time. This application of PHLIP presents the power of biofilm image analysis by multi-channel CLSM software and demonstrates the importance of PHLIP for the scientific community as a flexible and extendable image analysis platform for automated image processing

Teste alternativo para avaliação do potencial fisiológico de sementes de milho e feijão de porco.

bitstream/item/56906/1/COT89-lancado.pd

Brazilian scientific articles on “Spirituality, Religion and Health”

Abstract Background Studies on “Spirituality, religion and health” (R/S) have been increasing worldwide, including in Brazil. Mapping this production can help researchers to understand this field and also to identify gaps in the Brazilian R/S studies. Objective To analyze the Brazilian scientific articles on “Religion, Spirituality and Health” available on the main electronic databases using a bibliometric approach. Methods A comprehensive review of four major databases (PubMed, Scopus, BVS and Web of Science) was conducted. Three reviewers performed the data analysis. Off-topic articles, articles from Portugal, books and thesis were excluded. Articles were then classified by: Publication year, journal, Central focus in R/S, Academic Area, Main topic and Study Type. Results From 3,963 articles found, 686 studies were included in the final analysis (320 had central focus on R/S). There was an increase of articles in the last decade (most observational), with predominance of mental health issues, and from journals in the field of psychiatry, public health and nursing. Discussion This study enabled us to widen our understanding about how the field of “spirituality, religion and health” has been established and how this field is increasing in Brazil. These findings can help in the development of future Brazilian studies

PESQUISA PARTICIPATIVA DE BASE COMUNITÁRIA (PPBC): UMA METODOLOGIA PARA ESTUDOS DE PARTICIPAÇÃO DE ADOLESCENTES

O estudo objetiva apresentar a Pesquisa Participativa de Base Comunitária (PPBC) como metodologia utilizada em estudo de participação com adolescentes. A metodologia adotada aborda questões relacionadas ao estudo e a intervenção em determinado grupo de pessoas, a comunidade a ser estudada, que nesse estudo são os adolescentes. Para tanto, alguns princípios devem ser seguidos para que as vantagens do método sejam evidentes. A pesquisa, nesse estudo relatada, enfatizou a temática participação na promoção da saúde do adolescente em uma perspectiva mais abrangente, sendo desenvolvida com um grupo de adolescentes que vivenciaram momentos educativos/participativos, baseados nos princípios da PPBC. A pesquisa abrangeu seis encontros, cada um foi planejado juntamente com os adolescentes. A avaliação do processo realizou-se mediante a observação e percepções colhidas através de registros escritos e verbais, além das manifestações não verbais. Destacaram-se como pontos positivos na implementação da metodologia a consecução de autonomia e vínculo entre a comunidade estudada e a científica (adolescentes e pesquisadores), e o aprendizado de temas realizados a promoção da saúde do público adolescente. Evidenciou-se que esse tipo de metodologia adequou-se ao estudo com adolescentes, tendo em vista que os passos foram seguidos para o alcance do objeto do estudo

Biopsychosocial Aspects in Individuals with Acute and Chronic Rotator Cuff Related Shoulder Pain: Classification Based on a Decision Tree Analysis

Biopsychosocial aspects seem to influence the clinical condition of rotator cuff related shoulder pain (RCRSP). However, traditional bivariate and linear analyses may not be sufficiently robust to capture the complex relationships among these aspects. This study determined which biopsychosocial aspects would better classify individuals with acute and chronic RCRSP and described how these aspects interact to create biopsychosocial phenotypes in individuals with acute and chronic RCRSP. Individuals with acute (<six months of pain, n = 15) and chronic (≥six months of pain, n = 38) RCRSP were included. Sociodemographic data, biological data related to general clinical health status, to shoulder clinical condition and to sensory function, and psychosocial data were collected. Outcomes were compared between groups and a decision tree was used to classify the individuals with acute and chronic RCRSP into different phenotypes hierarchically organized in nodes. Only conditioned pain modulation was different between the groups. However, the tree combined six biopsychosocial aspects to identify seven distinct phenotypes in individuals with RCRSP: three phenotypes of individuals with acute, and four with chronic RCRSP. While the majority of the individuals with chronic RCRSP have no other previous painful complaint besides the shoulder pain and low efficiency of endogenous pain modulation with no signs of biomechanical related pain, individuals with acute RCRSP are more likely to have preserved endogenous pain modulation and unilateral pain with signs of kinesiophobia

Comorbid mood and anxiety disorders in victims of violence with posttraumatic stress disorder

OBJETIVO: Buscar estudos que avaliem a comorbidade entre transtorno de estresse pós-traumático e transtornos do humor, bem como entre transtorno de estresse pós-traumático e outros transtornos de ansiedade. MÉTODO: Revisamos a base de dados do Medline em busca de estudos publicados em inglês até abril de 2009, com as seguintes palavras-chave: "transtorno de estresse pós-traumático", "TEPT", "transtorno de humor", "transtorno depressivo maior", "depressão maior", "transtorno bipolar", "distimia", "transtorno de ansiedade", "transtorno de ansiedade generalizada", agorafobia", "transtorno obsessivo-compulsivo", "transtorno de pânico", "fobia social" e "comorbidade". RESULTADOS: Depressão maior é uma das condições comórbidas mais frequentes em indivíduos com transtorno de estresse pós-traumático, mas eles também apresentam transtorno bipolar e outros transtornos ansiosos. Essas comorbidades impõem um prejuízo clínico adicional e comprometem a qualidade de vida desses indivíduos. Comportamento suicida em pacientes com transtorno de estresse pós-traumático, com ou sem depressão maior comórbida, é também uma questão relevante, e sintomas depressivos mediam a gravidade da dor em sujeitos com transtorno de estresse pós-traumático e dor crônica. CONCLUSÃO: Os estudos disponíveis sugerem que pacientes com transtorno de estresse pós-traumático têm um risco maior de desenvolver transtornos afetivos e, por outro lado, transtornos afetivos pré-existentes aumentam a propensão ao transtorno de estresse pós-traumático após eventos traumáticos. Além disso, vulnerabilidades genéticas em comum podem ajudar a explicar esse padrão de comorbidades. No entanto, diante dos poucos estudos encontrados, mais trabalhos são necessários para avaliar adequadamente essas comorbidades e suas implicações clínicas e terapêuticas.OBJECTIVE: To review studies that have evaluated the comorbidity between posttraumatic stress disorder and mood disorders, as well as between posttraumatic stress disorder and other anxiety disorders. METHOD: We searched Medline for studies, published in English through April, 2009, using the following keywords: "posttraumatic stress disorder", "PTSD", "mood disorder", "major depressive disorder", "major depression", "bipolar disorder", "dysthymia", "anxiety disorder", "generalized anxiety disorder", "agoraphobia", "obsessive-compulsive disorder", "panic disorder", "social phobia", and "comorbidity". RESULTS: Major depression is one of the most frequent comorbid conditions in posttraumatic stress disorder individuals, but individuals with posttraumatic stress disorder are also more likely to present with bipolar disorder, other anxiety disorders and suicidal behaviors. These comorbid conditions are associated with greater clinical severity, functional impairment, and impaired quality of life in already compromised individuals with posttraumatic stress disorder. Depression symptoms also mediate the association between posttraumatic stress disorder and severity of pain among patients with chronic pain. CONCLUSION: Available studies suggest that individuals with posttraumatic stress disorder are at increased risk of developing affective disorders compared with trauma-exposed individuals who do not develop posttraumatic stress disorder. Conversely, pre-existing affective disorders increase a person's vulnerability to the posttraumatic stress disorder--inducing effects of traumatic events. Also, common genetic vulnerabilities can help to explain these comorbidity patterns. However, because the studies addressing this issue are few in number, heterogeneous and based on a limited sample, more studies are needed in order to adequately evaluate these comorbidities, as well as their clinical and therapeutic implications

Evolving Convolutional Neural Networks for Glaucoma Diagnosis / Redes neurais convolucionais em evolução para diagnóstico de glaucoma

O glaucoma é uma doença ocular que causa danos ao nervo óptico do olho e sucessivo estreitamento do campo visual nos pacientes afetados, o que pode levar o paciente, em estágio avançado, à cegueira. Este trabalho apresenta um estudo sobre o uso de Redes Neurais Convolucionais (CNNs) para o diagnóstico automático através de imagens de fundo de olho. No entanto, a construção de uma CNN capaz de alcançar resultados satisfatórios para o diagnóstico do glaucoma, envolve muito esforço que, em muitas situações, nem sempre é capaz de tais resultados. O objetivo deste trabalho é utilizar um algoritmo genético (AG) para otimizar arquiteturas de CNNs através da técnica de evolução de algoritmos que possa aprimorar o diagnóstico do glaucoma em imagens de fundo do olho do conjunto de dados RIM-ONE-r2. Nosso artigo demonstra resultados satisfatórios após o treinamento do melhor indivíduo escolhido pelo AG, com a obtenção de uma acurácia de 91%

Synthesis, surface active and antimicrobial properties of new alkyl 2,6-dideoxy-L-arabino-hexopyranosides

Synthesis of alkyl 2,6-dideoxy-L-arabino-hexopyranosides was accomplished by the reaction of 1,5-anhydro-2,6-dideoxy-L-arabino-hex-1-enitol with fatty alcohols in dichloromethane, catalyzed by triphenylphosphine hydrobromide. Reaction with octanol and dodecanol gave the corresponding α-glycosides in 50% and 42% yield, the β-glycosides in 20% and 21% yield and the α-anomer of the Ferrier product in 10% and 9% yield, respectively.Deacetylation of the α-/β-glycosides with sodium methoxide in methanol afforded the amphiphilic L-arabino-hexopyranosides in 94–99% yield. The surface tension at the air–water interface of the octyl L-glycosides and of the dodecyl α-L-glycoside aqueous solutions at 35 °C was measured with a du Noüy ring tensiometer and surface properties such as critical micelle concentration (CMC), relative surface excess, molecular area at the interface and Gibbs micellization free energy were evaluated. The stereochemistry of the hexopyranoside ring in unimers and aggregates is correlated to the hydrophobicity and packing efficiency on the air–water interface. The antibacterial and antifungal activities of the surface-active glycosides were evaluated using the paper disk diffusion method. The dodecyl α-L-arabino-hexopyranoside was quite active over Bacillus cereus and Bacillus subtilis, while low activity was found for this glycoside over Enterococcus faecalis and Listeria monocytogenes. The octyl glycosides tested showed low activity over almost all the above-mentioned bacteria, and also over the fungus Candida albicans. No inhibition of Salmonella enteritidis and of the filamentous fungus Aspergillus niger was detected for any of the compounds tested.info:eu-repo/semantics/publishedVersio

Envenenamento botrópico em bovinos: tratamento opcional.

Envenenamento botropico em bovinos. Envenenamento por B. alternatus, B. neuwiedi, B. moojeni, B. jararacussu, B. jararaca, B.atrox.bitstream/item/146781/1/CT03-Envenenamento-botropico-em-bovinos-1986.pd

Melatonin activate FIS1, DYN1 and DYN2 Plasmodium falciparum related-genes for mitochondria fission: mitoemerald-GFP as a tool to visualize mitochondria structure

Malaria causes millions of deaths worldwide and is considered a huge burden to underdeveloped countries. The number of cases with resistance to all antimalarials is continuously increasing, making the identification of novel drugs a very urgent necessity. A potentially very interesting target for novel therapeutic intervention is the parasite mitochondrion. In this work we studied in P. falciparum three genes coding for proteins homologues of the mammalian FIS1 (Mitochondrial Fission Protein 1) and DRP1 (Dynamin Related Protein 1) involved in mitochondrial fission. We studied the expression of P. falciparum genes that show ample sequence and structural homologies with the mammalian counterparts, namely FIS1, DYN1 and DYN2. The encoded proteins are characterized by a distinct pattern of expression throughout the erythrocytic cycle of Plasmodium falciparum and their mRNAs are modulated by treating the parasite with the host hormone melatonin. We have previously reported that the knock out of the Plasmodium gene that codes for protein kinase 7 is essential for melatonin sensing. We here show that PfPk7 knockout results in major alterations of mitochondrial fission genes expression when compared to wild type parasites, and no change in fission protein expression upon treatment with the host hormone. Finally we have compared the morphological characteristics(using MitoTracker Red CMX Ros. and oxygen consumption properties of P. falciparum mitochondria in wild type parasites and PfPk7 Knockout strains. A novel GFP construct targeted to the mitochondrial matrix to wild type parasites was also developed to visualize Plasmodium falciparum mitochondria. We here show that, the functional characteristics of P. falciparum are profoundly altered in cells lacking protein kinase 7, suggesting that this enzyme plays a major role in the control of mitochondrial morphogenesis and maturation during the intra erythrocyte cell cycle progression. This article is protected by copyright. All rights reserved