Aging: a portrait from gene expression profile in blood cells

The availability of reliable biomarkers of aging is important not only to monitor the effect of interventions and predict the timing of pathologies associated with aging but also to understand the mechanisms and devise appropriate countermeasures. Blood cells provide an easily available tissue and gene expression profiles from whole blood samples appear to mirror disease states and some aspects of the aging process itself. We report here a microarray analysis of whole blood samples from two cohorts of healthy adult and elderly subjects, aged 43 +/- 3 and 68 +/- 4 years, respectively, to monitor gene expression changes in the initial phase of the senescence process. A number of significant changes were found in the elderly compared to the adult group, including decreased levels of transcripts coding for components of the mitochondrial respiratory chain, which correlate with a parallel decline in the maximum rate of oxygen consumption (VO2max), as monitored in the same subjects. In addition, blood cells show age-related changes in the expression of several markers of immunosenescence, inflammation and oxidative stress. These findings support the notion that the immune system has a major role in tissue homeostasis and repair, which appears to be impaired since early stages of the aging process

Preliminary tests to design an ad hoc signal acquisition chain for the Sardinia Aperture Array Demonstrator

The Sardinia Aperture Array Demonstrator (SAD) is an Italian facility, which is composed of 128 prototypical Vivaldi antennas, specifically designed to operate across the 50-500 MHz frequency range. As well known, one of the major burden at low frequency are the radio frequency interferences, thus after several accurate measurement campaigns we realized that a specific signal conditioning is needed in order to feed the digital beamformer with the proper signal level. In this paper, we report the results of the preliminary tests that we carried out in order to design an ad hoc receiving chain for the SAD array

MRI versus mammography plus ultrasound in women at intermediate breast cancer risk: study design and protocol of the MRIB multicenter, randomized, controlled trial

In women at high/intermediate lifetime risk of breast cancer (BC-LTR), contrast-enhanced magnetic resonance imaging (MRI) added to mammography ± ultrasound (MX ± US) increases sensitivity but decreases specificity. Screening with MRI alone is an alternative and potentially more cost-effective strategy. Here, we describe the study protocol and the characteristics of enrolled patients for MRIB feasibility, multicenter, randomized, controlled trial, which aims to compare MRI alone versus MX+US in women at intermediate breast cancer risk (aged 40-59, with a 15-30% BC-LTR and/or extremely dense breasts). Two screening rounds per woman were planned in ten centers experienced in MRI screening, the primary endpoint being the rate of cancers detected in the 2 arms after 5 years of follow-up. From July 2013 to November 2015, 1254 women (mean age 47 years) were enrolled: 624 were assigned to MX+US and 630 to MRI. Most of them were aged below 50 (72%) and premenopausal (45%), and 52% used oral contraceptives. Among postmenopausal women, 15% had used hormone replacement therapy. Breast and/or ovarian cancer in mothers and/or sisters were reported by 37% of enrolled women, 79% had extremely dense breasts, and 41% had a 15-30% BC-LTR. The distribution of the major determinants of breast cancer risk profiles (breast density and family history of breast and ovarian cancer) of enrolled women varied across centers

Exploration of an innovative ranging method for bi-static radar, applied in LEO Space Debris surveying and tracking

Space Situational Awareness (SSA) is referred as one of the capacitive areas of strategic interest to be developed/completed in the future in the short and medium term, for any nation with the target of the access to the space. One of the fundamental components is the Space Surveillance and Tracking (SST) program, considered as the capability to build a spatial mapping of the objects in orbit, their classification and the exact identification of their orbital characteristics. For this reason, radar measurements are relevant, in particular to observe objects in Low Earth Orbit. The Italian National Institute of Astrophysics together with Vitrociset company and Politecnico di Milano, studied and developed a new and innovative method for the range measure applied to bi-static radars to support the European Union Space Surveillance and Tracking (EUSST) program. Several tests have been carried out using the BIRALES and BIRALET sensors for survey and tracking observations respectively. Finally, the results obtained from observations have been compared with the real positions of the targets in order to validate the system. The ranging method relies on the synchronization of the transmitting and receiving antennas and on the correlation of the echo received from the scattering of the orbiting object. To do that, the transmitting antenna emits simultaneously two different signals: a Chirp signal for range measurement and a second “Continuous Wave” (CW) for Doppler shift measurement and object track reconstruction. Overall, we simultaneously obtain time profiles for range, angular position (azimuth and elevation), and Doppler during the passage of the objects inside the sensor Field of View. By virtue of the above plethora of measurements, this method guarantees also the possibility to produce an Initial Orbital Determination (IOD) for unknown objects

Exploitation of bi-static radar architectures for LEO Space Debris surveying and tracking: The BIRALES/BlRALET project

The space debris population is continuously growing and it represents a potential issue for spacecraft. New collisions could exponentially rise the amount of debris and so the level of risk represented by these objects. The monitoring of space environment is necessary to prevent new collisions. For this reason, radar measurements are relevant, in particular to observe objects in Low Earth Orbit. Regarding the Italian contribution, there are two radars based on two different radio telescopes as receivers: the BIRALES and the BIRALET systems. We propose a detailed description of these systems, focusing on hardware and software components that permit to perform range and range rate measurement of resident space objects

Persistent cAMP-Signals Triggered by Internalized G-Protein–Coupled Receptors

Real-time monitoring of G-protein-coupled receptor (GPCR) signaling in native cells suggests that the receptor for thyroid stimulating hormone remains active after internalization, challenging the current model for GPCR signaling

Image-guided breast biopsy and localisation: recommendations for information to women and referring physicians by the European Society of Breast Imaging

Abstract: We summarise here the information to be provided to women and referring physicians about percutaneous breast biopsy and lesion localisation under imaging guidance. After explaining why a preoperative diagnosis with a percutaneous biopsy is preferred to surgical biopsy, we illustrate the criteria used by radiologists for choosing the most appropriate combination of device type for sampling and imaging technique for guidance. Then, we describe the commonly used devices, from fine-needle sampling to tissue biopsy with larger needles, namely core needle biopsy and vacuum-assisted biopsy, and how mammography, digital breast tomosynthesis, ultrasound, or magnetic resonance imaging work for targeting the lesion for sampling or localisation. The differences among the techniques available for localisation (carbon marking, metallic wire, radiotracer injection, radioactive seed, and magnetic seed localisation) are illustrated. Type and rate of possible complications are described and the issue of concomitant antiplatelet or anticoagulant therapy is also addressed. The importance of pathological-radiological correlation is highlighted: when evaluating the results of any needle sampling, the radiologist must check the concordance between the cytology/pathology report of the sample and the radiological appearance of the biopsied lesion. We recommend that special attention is paid to a proper and tactful approach when communicating to the woman the need for tissue sampling as well as the possibility of cancer diagnosis, repeat tissue sampling, and or even surgery when tissue sampling shows a lesion with uncertain malignant potential (also referred to as “high-risk” or B3 lesions). Finally, seven frequently asked questions are answered

Synthesis and study of supramolecular catalyst based on the salophen-UO2 unit

Dottorato di ricerca in scienze chimiche. Relatore L. MandoliniConsiglio Nazionale delle Ricerche - Biblioteca Centrale - P.le Aldo Moro, 7, Rome; Biblioteca Nazionale Centrale - Piazza Cavalleggeri, 1, Florence / CNR - Consiglio Nazionale delle RichercheSIGLEITItal

Mechanistic Insights into the Soai Reaction from Formal Kinetics and Density Functional Theory Calculations

The Blackmond-Brown dimer model (DM) is one of the most credited mechanism proposed to explain the asymmetric autocatalysis in the Soai reaction. It is based on the hypothesis that the homochiral dimers of the zinc alkoxide product are the catalitically active species in statistical equilibrium with the inactive heterochiral dimer. In this chapter, we show that the OM model may be viewed as a particular case of an "extended dimer model", the outcome of which depends on the result of the competition between a homochiral homocatalytic cycle and two other catalytic cycles, the homochiral enantiocatalytic and the heterocatalytic ones. We also present a thorough DFT computational study on the mechanism of the Soai reaction, aimed at locating the relevant transition states of the catalytic cycles. On the basis of computational results, a mechanism detailed at the molecular level is proposed, that sheds light on the origin of enantioselectivity and lends support to the main hypotheses of the DM. This mechanism reproduces the evolution of enantiomeric excess (ee) over the reaction progress at ambient temperature, i.e. as long as no significant formation of oligomers higher than the dimers takes place in solution