Determinación de selénio por su efecto catalítico en la reducción del azul de toluidina por el ion sulfuro

The reduction of toluidine blue by sulphide is catalysed by small amounts of selenium. As a consequence a new kinetic method for the selenium determination is developed. The experimental conditions have been optimized and the variable time method appears to be the most suitable for the determination of selenium. The new method is very sensitive (10 ng/ml) showing good accuracy. Most of the interferences from diverse metallic ions are eliminated by the addition of an excess of Ba(II)-EDTA.La reducción del azul de toluidina por el sidfuro es catalizada cuando se encuentran presentes pequeñas cantidades de selenio. Como consecuencia de esto aportamos un nuevo método cinético para la determinación de selenio. Las condiciones experimentales han sido optimizadas y el método cinético de tiempo variable se seleccionó como más conveniente para las determinaciones analíticas de selenio. El nuevo procedimiento es muy sensible (10 ng/ml) y presenta buena exactitud y precisión. La mayoría de las interferencias producidas por 'los iones metálicos se eliminan mediante la adición de un exceso de AEDT-Ba2

Oxidaciones de leucocolorantes derivados del trifenil metano con cloramina T, catalizadas por el ioduro. Determinaciones de ioduro, plata, mercurio y paladio

The oxidation of leuco crystal violet and of leuco malachite green by chloramine T is catalysed by iodide. Two sensitive, accurate, selective and rapid methods for the determination of iodide are proposed. Ag(I), Hg(II) and Pd(II) ions under suitable conditions inhibit the catalytic effect of iodide on oxidation of reduced form of both dyes by chloramine T. The kinetic determination of these metallic ions is useful at the trace range.La oxidación del leucovioleta cristal y leucoverde malaquita por la cloramina T es catalizada por el ioduro. Basados en este efecto se proponen dos métodos para la determinación de ioduro sensibles, exactos, selectivos y rápidos. En condiciones adecuadas los iones Ag(I), Hg(II) y Pd(II) inhiben la acción catalítica del ioduro sobre la oxidación de las formas reducidas de ambos colorantes por la cloramina T. Las determinaciones cinéticas de estos iones metálicos son aplicables en rangos de concentración a niveles de trazas

Associations between inter-limb asymmetries in jump and change of direction speed tests and physical performance in adolescent female soccer players

The association between asymmetries in jump and change of direction (COD) with physical performance in several sports show inconclusive results. The purposes of this study were to: (1) measure inter-limb asymmetries in three distinct groups in adolescent female soccer players and, (2) to determine the association between inter-limb asymmetries and physical performance in different age groups. Fifty-four players were distributed in three age groups: U-18, U-16 and U-14. All of them performed a series of jumps, sprints and change of direction speed tests. Asymmetries were assessed as the percentage difference between limbs, with the equation: 100/Max value (right and left) * in value (right and left) * −1 + 100. Mean inter-limb asymmetries were 2.91%, 4.82% and 11.6% for 180° COD, single leg hop and single leg countermovement jump tests respectively, but higher percentages of asymmetries were observed in many players individually. U-18 and U-16 showed significant differences on 180° left COD compared to U-14. Effect size (ES): 0.80 and 0.74, respectively; U-18 presented differences on single left leg hop test compared to U-14, ES: −0.72; U-16 also showed differences on 40 m speed compared to U-14, ES 0.87 (All p 0.05). Jumping and COD physical tests show asymmetries in adolescent female soccer players, but these asymmetries do not interfere with physical performance. The largest asymmetry was observed in the single leg countermovement jump, and no asymmetries between groups were found. Due to the high variability in the direction of asymmetries, it is recommended to consider players’ individual asymmetries for designing specific training programs

Túnel de Viento para el Estudio de la Erosión Eólica

Número de publicación: ES2453815 A1 (08.04.2014) También publicado como: ES2453815 B1 (20.01.2015) Número de Solicitud: Consulta de Expedientes OEPM (C.E.O.) P201201028 (08.10.2012)Túnel de viento para el estudio de la erosión eólica, caracterizado por ser ligero y plegable, donde un ventilador genera una corriente de aire, que entra en una estructura "tubular" desplegable. La estructura es telescópica. Se divide en 3 módulos y se han colocado unas bisagras, que permiten transformarlo en un cubo completamente plegado de reducidas dimensiones. Para completar el conjunto telescópico, se ha diseñado un sistema que permite a las paredes adaptarse a ésta estructura, utilizando patines que deslizan sobre un raíl. La recogida y análisis de las muestras, se realiza con 2 sensores: 1. Un láser-scan, que permite la generación de mapas tridimensionales del suelo, antes y después de ser erosionado. 2. Una cámara de visión industrial, que capta imágenes de las partículas fijadas en una serie de placas con adhesivos, colocadas al final del dispositivo.Universidad de Almerí

Cutaneous mucormycosis

Cutaneous mucormycosis is an emerging fungal infection caused by opportunistic fungi of the phylum Glomeromycota. It is frequent in poorly controlled diabetic patients and individuals with immunosuppression. It is usually acquired by direct inoculation through trauma. The clinical presentation is nonspecific, but an indurated plaque that rapidly evolves to necrosis is a common finding. Diagnosis should be confirmed by demonstration of the etiological agent and new molecular diagnostic tools have recently been described. It is an invasive life-threatening disease and in order to improve survival, a prompt diagnosis and multidisciplinary management should be provided. The treatment of choice is amphotericin B, but new azoles, such as posaconazole and isavuconazole, must be considered. Keywords: Mucormycosis; Mucorales; Mucor; Zygomycosis; Rhizopu

Regulatory effects of miR-19a on MAD2 expression and tumorigenesis in gastric cancer

Gastric cancer (GC) is worldwide the sixth most diagnosed and third leading cause of cancer deaths, with poor and late prognosis, probably due to post-surgery adjuvant treatment resistance and lack of a thorough panel of prognostic markers. We have previously shown that mitotic arrest deficient 2 (MAD2, encoded by MAD2L1), a key protein of the spindle assembly checkpoint, is relevant in GC cells; its interference impairs migration and growth, while its overexpression correlates with tumorigenesi

Aplicaciones analíticas de la reducción fotoquímica de la riboflavina el ácido etilenodiaminotetraacético. Determinación de ioduro

A kinetic study of the photochemical reaction of riboflavin and EDTA (in the absence of oxygen) has been made in connection with development of a new kinetic method for iodide. The reaction is first-order with respect to riboflavin, EDTA and absorbed light-intensity. The rate of photoreduction is strongly pH^dependent, and maximal at about pH 7,5. The photoreduction involves a long-Uved excited state of the vitamin B2 and is dramaticalUy retarded by small amounts of iodide. A tenta'tive mechanism is proposed, and the experimental conditions have been optimized. The variable time method appears to be the most suitable for determination of iodide. A detection limit of 8 [xg/rnl and a casfficient of variation about 3 % can be adhieved. Chloride and bromide do not interfere at levéis below 100-fold mole ratio to iodide. Metal ions do not interfere if enough excess of EDTA is used.Se ha estudiado la reacción fotoquímica entre la riboflavina y el ácido etilenodiaminotetraacético (en ausencia de oxígeno) con la finalidad de desarrollar un método cinético para la determinación de ioduro. La reacción es de primer orden con respecto a la riboflavina, AEDT e intensidad de la radiación absorbida. La velocidad del proceso fotoquímico depende del pH y es máxima a pH 7,2. La fotorreducción tiene lugar a través de estados excitados de larga vida de la vitamina B2 y está muy afectada por la presencia de pequeñas cantidades de ioduro. Se propone un posible mecanismo y se han estudiado todas las variables con la finalidad de optimizar las condiciones del método analítico. Se ha empleado el procedimiento del tiempo variable como el más apropiado para la determinación de ioduro. La sensibilidad es de 8 fig/ml y el coeficien'te de variación del 3 %. Los iones bromuro y cloruro son compatibles hasta la relación 100/1. La interferencia de los iones metálicos se elimina añadiendo un exceso de AEDT

Evaluation of bacterial adherence of clinical isolates of Staphylococcus sp. using a competitive model: An in vitro approach to the "race for the surface" theory

Objectives Implant-related infection is one of the most devastating complications in orthopaedic surgery. Many surface and/or material modifications have been developed in order to minimise this problem; however, most of the in vitro studies did not evaluate bacterial adhesion in the presence of eukaryotic cells, as stated by the 'race for the surface' theory. Moreover, the adherence of numerous clinical strains with different initial concentrations has not been studied. Methods We describe a method for the study of bacterial adherence in the presence of preosteoblastic cells. For this purpose we mixed different concentrations of bacterial cells from collection and clinical strains of staphylococci isolated from implant-related infections with preosteoblastic cells, and analysed the minimal concentration of bacteria able to colonise the surface of the material with image analysis. Results Our results show that clinical strains adhere to the material surface at lower concentrations than collection strains. A destructive effect of bacteria on preosteoblastic cells was also detected, especially with higher concentrations of bacteria. Conclusions The method described herein can be used to evaluate the effect of surface modifications on bacterial adherence more accurately than conventional monoculture studies. Clinical strains behave differently than collection strains with respect to bacterial adherence.This work was funded by the following grants from the Spanish MINECO (MAT2013- 48224-C2-2-R and MAT2013-48224-C2-1-R). M. Martínez-Pérez reports funding received from EFORT 2015 congress: travel supported by PFIZER, which is related to this article. J. Esteban and E. Gómez-Barrena report funding received from several companies for travel, expenses and grants, none of which is related to this articl