First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina

Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.Facultad de Ciencias Veterinaria

First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina

Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.Facultad de Ciencias Veterinaria

First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina

Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.Fil: Serena, Maria Soledad. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Metz, German Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaFil: Lozada, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria "Dr. Bernardo Epstein"; ArgentinaFil: Aspitia, Carolina Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria "Dr. Bernardo Epstein"; ArgentinaFil: Nicolino, Edgardo Hector. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Departamento de Enfermedades Infecciosas; ArgentinaFil: Pidone, Claudio Luis. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Departamento de Enfermedades Infecciosas; ArgentinaFil: Fossaroli, Melisa Gisele. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Departamento de patología. ; ArgentinaFil: Balsalobre, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Quiroga, María Alejandra. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Departamento de patología. ; ArgentinaFil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentin

First detection and genetic characterization of porcine circovirus type 3 (PCV3) in Argentina and its association with reproductive failure

Porcine circovirus type 3 (PCV3) is considered a new circovirus and since it first description has been widely reported in most of the swine-producing countries. Multisystemic inflammation and reproductive failure are consistent and concerning issues associated with PCV3 infection. This report describes the clinical and pathological features of a chronic reproductive disorder in a swine herd in Argentina associated with the presence of PCV3. Mummified (n = 42) and stillborn piglets (n = 20) from a case of chronic reproductive disorder (Study A) and mummified and stillborn piglets (n = 141) from normal deliveries (Study B) were retrospectively assessed for the presence of multiple reproductive pathogens (PCV3, PCV2, ADV, PPV, Leptospira spp. and Brucella spp). On study, A PCV3 and PPV were detected in 15 and 8 pools, respectively, with a coinfection rate of 100% in all PPV-positive cases. Three out of 131 foetuses from three different sows from Study B were positive only for PCV3. Histological evaluation of hearts from stillborn also showed lesions similar to those previously described in the literature for PCV3-reproductive disease. Partial genome of PCV3 was amplified and phylogenetic analysis showed that strains of Study A and B clustered within the PCV3a and PCV3b clades, respectively. This study demonstrates, for the first time, the PCV3 has been circulating in Argentina at least since 2016 and its potential role in reproductive disorders. Further studies are warranted to determine the role of PCV3 in the reproductive disease complex and its prevalence in the swine industry in Argentina.Fil: Serena, Maria Soledad. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Cappuccio, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Barrales, Hernán. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Metz, German Ernesto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Aspitia, Carolina Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Cátedra de Patología Especial Veterinaria; ArgentinaFil: Lozada, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Perfumo, Carlos Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Cátedra de Patología Especial Veterinaria; ArgentinaFil: Quiroga, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Cátedra de Patología Especial Veterinaria; ArgentinaFil: Piñeyro Piñeiro, Pablo Enrique. University of Iowa; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Echeverria, Maria Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentin

First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina

Background: (TGEV) was made by the NationalServices of Animal Health of Argentina (SENASA) to the World Organization of Animal Health (OIE). The notificationwas based on a serological diagnosis in a small farm with a morbidity rate of 2.3% without enteric clinical signs. Inorder to determine if TGEV was circulating before the official report, a retrospective study on cases of neonataldiarrhea was performed. The selection criteria was a sudden increase in mortality in 1- to 21-day-old piglets withwatery diarrhea that did not respond to antibiotics. Based on these criteria, three clinical cases were identifiedduring 2010?2015.Results: All animals that were evaluated presented histological lesions consistent with enteric viral infection. Thefeces and ultrathin sections of intestine that were evaluated by electron microscopy confirmed the presence ofround particles of approximately 80 nm in size and characterized by finely granular electrodense nucleoidsconsistent with complete particles of coronavirus. The presence of the TGEV antigen was confirmed by monoclonalspecific immunohistochemistry, and final confirmation of a metabolically-active virus was performed by in situhybridization to detect a TGE mRNA encoding spike protein. All sections evaluated in this case were negative forPEDV and rotavirus A.Conclusions: This is the first case series describing neonatal mortality with etiological confirmation of TGEV inArgentina. The clinical diagnosis of TGEV infections in endemic regions is challenging due to the epidemiologicaldistribution and coinfection with other enteric pathogens that mask the clinical presentation.Fil: Piñeyro Piñeiro, Pablo Enrique. University Of Iowa. Ames Laboratory; Estados Unidos. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Cátedra de Patología Especial Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lozada, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria "Dr. Bernardo Epstein"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alarcon, Laura Valeria. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Sanguinetti, Ramon. Ministerio de Agricultura, Ganadería, Pesca y Alimento; ArgentinaFil: Cappuccio, Javier Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Pérez, Estefanía Marisol. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vannucci, Fabio. University Of Minnesota. School Of Public Health.; Estados UnidosFil: Armocida, Alberto Domingo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Madson, Darin Michael. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; ArgentinaFil: Perfumo, Carlos Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; ArgentinaFil: Quiroga, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; Argentin

Évaluation de la pneumonie porcine à l’abattage en utilisant la réaction d’amplification en chaine par la polymérase et l’histopathologie en Argentine

Se realizó la reacción en cadena de polimerasa e histopatología en 81 pulmones recolectados en el matadero de 13 granjas porcinas libres del síndrome reproductivo y respiratorio porcino, y de la infección por el virus de la pseudorabia. La Pasteurella multocida y el Mycoplasma hyopneumoniae fueron los patógenos más comúnmente detectados. La bronconeumonía supurativa y catarral estuvieron presentes en 59 (72.8%) casos.Histopathology and polymerase chain reaction were conducted on 81 lungs collected at slaughter from 13 swine farms free of porcine reproductive and respiratory syndrome virus and pseudorabies virus infection. Pasteurella multocida and Mycoplasma hyopneumoniae were the most common pathogens detected. Suppurative and catarrhal bronchopneumonia was present in 59 (72.8%) cases.L’histopathologie et la réaction d’amplification en chaine par la polymérase ont été réalisées sur 81 poumons récoltés à l’abattoir provenant de 13 fermes porcines exemptes du virus du syndrome reproducteur et respiratoire porcin et d’infection par le virus de la pseudorage. Pasteurella multocida et Mycoplasma hyopneumoniae étaient les agents pathogènes les plus communément détectés. Une bronchopneumonie suppurative et catarrhale était présente dans 59 (72.8%) cas.Fil: Cappuccio, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dibárbora, Marina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bessone, Fernando Anibal. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Olivera, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Lozada, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Cátedra de Patología Especial Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Quiroga, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Cátedra de Patología Especial Veterinaria; ArgentinaFil: Pérez, Estefanía Marisol. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Cátedra de Patología Especial Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zielinski, Gustavo Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Perfumo, Carlos Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Cátedra de Patología Especial Veterinaria; ArgentinaFil: Pereda, Ariel Julián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pérez, Daniel R.. University of Georgia; Estados Unido

La imagen y la narrativa como herramientas para el abordaje psicosocial en escenarios de violencia Departamentos Amazonas, Boyacá, Meta y Vichada.

El presente documento se realiza a partir del análisis de las subjetividades, de los casos de Jennifer Pinzón y el Municipio de Panduri, los cuales son una muestra de las miles de historias del pueblo Colombiano, debido a su guerra interna de más de 50 años, donde han sido afectados distintas generaciones, de forma directa o indirecta, afectando desde la salud mental y física, como la estabilidad económica y democrática del país, sin embargo con las nuevas políticas se pretende lograr encontrar la paz, pero para llegar a esta meta es necesario un periodo de transición al cual se le llama posconflicto, el cual requiere de personal capacitado para desarrollar proyectos sociales que ayuden a reconstruir el tejido social que se ha perdido a causa de este conflicto interno. Es por esto que mediante el acercamiento con la realidad del país y el análisis detallado de los casos propuestos para este fin, se pretende generar un aprendizaje significativo de los temas propuestos durante este diplomado, ya que durante del desarrollo de este documento, se podrán a pruebas los conocimientos adquiridos, desde el análisis hasta una propuesta de intervención social, lo cual permite desarrollar nuevas competencias en los estudiantes, formando profesionales capacitados en intervención de comunidades que han sido víctimas de la violencia o victimarios.This document is carried out starting from the analysis of the subjectivities, of the cases of Jennifer Pinzon and the Municipality of Panduri, which is an example of the thousands of histories of the Colombian town, do its internal war of more than 50 years, where different generations have been affected, in a direct or indirect way, affecting from the mental and physical health, as the economic and democratic stability of the country, however with the new norms it is sought to be able to find the peace, but to arrive to this goal it is necessary a period of transition to which is called post conflict, which requires of personnel qualified to develop social projects that will help to reconstruct the social fabric that has gotten lost because of this internal conflict. Is because of this that the approach with the reality of the country and the detailed analysis of the cases proposed for this which, we pretend to generate a significant learning of the topics proposed during this grade, since during of the development of this document, they will be able to tests the acquired knowledge, from the analysis until a proposal of social intervention, that which allows to develop new competitions in the students, forming professionals qualified in intervention of communities that have been victims of the violence at the country

First detection and genetic characterization of porcine circovirus type 3 (PCV3) in Argentina and its association with reproductive failure

Porcine circovirus type 3 (PCV3) is considered a new circovirus and since it first description has been widely reported in most of the swine-producing countries. Multisystemic inflammation and reproductive failure are consistent and concerning issues associated with PCV3 infection. This report describes the clinical and pathological features of a chronic reproductive disorder in a swine herd in Argentina associated with the presence of PCV3. Mummified (n = 42) and stillborn piglets (n = 20) from a case of chronic reproductive disorder (Study A) and mummified and stillborn piglets (n = 141) from normal deliveries (Study B) were retrospectively assessed for the presence of multiple reproductive pathogens (PCV3, PCV2, ADV, PPV, Leptospira spp. and Brucella spp). On study, A PCV3 and PPV were detected in 15 and 8 pools, respectively, with a coinfection rate of 100% in all PPV-positive cases. Three out of 131 foetuses from three different sows from Study B were positive only for PCV3. Histological evaluation of hearts from stillborn also showed lesions similar to those previously described in the literature for PCV3-reproductive disease. Partial genome of PCV3 was amplified and phylogenetic analysis showed that strains of Study A and B clustered within the PCV3a and PCV3b clades, respectively. This study demonstrates, for the first time, the PCV3 has been circulating in Argentina at least since 2016 and its potential role in reproductive disorders. Further studies are warranted to determine the role of PCV3 in the reproductive disease complex and its prevalence in the swine industry in Argentina.Facultad de Ciencias Veterinaria

First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina

Background: In 2014, a notification of porcine transmissible gastroenteritis virus (TGEV) was made by the National Services of Animal Health of Argentina (SENASA) to the World Organization of Animal Health (OIE). The notification was based on a serological diagnosis in a small farm with a morbidity rate of 2.3% without enteric clinical signs. In order to determine if TGEV was circulating before the official report, a retrospective study on cases of neonatal diarrhea was performed. The selection criteria was a sudden increase in mortality in 1- to 21-day-old piglets with watery diarrhea that did not respond to antibiotics. Based on these criteria, three clinical cases were identified during 2010–2015. Results: All animals that were evaluated presented histological lesions consistent with enteric viral infection. The feces and ultrathin sections of intestine that were evaluated by electron microscopy confirmed the presence of round particles of approximately 80 nm in size and characterized by finely granular electrodense nucleoids consistent with complete particles of coronavirus. The presence of the TGEV antigen was confirmed by monoclonal specific immunohistochemistry, and final confirmation of a metabolically-active virus was performed by in situ hybridization to detect a TGE mRNA encoding spike protein. All sections evaluated in this case were negative for PEDV and rotavirus A. Conclusions: This is the first case series describing neonatal mortality with etiological confirmation of TGEV in Argentina. The clinical diagnosis of TGEV infections in endemic regions is challenging due to the epidemiological distribution and coinfection with other enteric pathogens that mask the clinical presentation.Facultad de Ciencias Veterinaria

Two years of surveillance of influenza a virus infection in a swine herd : Results of virological, serological and pathological studies

Swine farms provide a dynamic environment for the evolution of influenza A viruses (IAVs). The present report shows the results of a surveillance effort of IAV infection in one commercial swine farm in Argentina. Two cross-sectional serological and virological studies (n = 480) were carried out in 2011 and 2012. Virus shedding was detected in nasal samples from pigs from ages 7,21 and 42-days old. More than 90% of sows and gilts but less than 40% of 21-days old piglets had antibodies against IAV. Inaddition, IAV was detected in 8/17 nasal swabs and 10/15 lung samples taken from necropsied pigs. A subset of these samples was further processed for virus isolation resulting in 6 viruses of the H1N2 subtype (δ2 cluster). Pathological studies revealed an association between suppurative bronchopneumonia and necrotizing bronchiolitis with IAV positive samples. Statistical analyses showed that the degree of lesions in bronchi, bronchiole, and alveoli was higher in lungs positive to IAV. The results of this study depict the relevance of continuing long-term active surveillance of IAV in swine populations to establish IAV evolution relevant to swine and humans.Facultad de Ciencias Veterinaria