Os provérbios estão vivos no Algarve

A obra em apreço é uma colectânea de 3638 provérbios, 1211 “expressões, máximas e comparações proverbiais” e 147 “provérbios em rimas e rimas com provérbios”, recolhidos na freguesia de Paderne, concelho de Albufeira. O volume foi coordenado por José Ruivinho Brazão, que contou com a colaboração de Dulcelina M. Coelho da Silva, Maria Isabel de Oliveira Maia Lima e Maria Solange P. Coelho da Silva. Para além do vasto material compilado, a obra integra ainda um Prefácio de Arnaldo Saraiva, uma Introdução extensa e um Índice remissivo da responsabilidade de Ruivinho Brazão

O cuidado à pessoa com cancro : experiência de estudantes de enfermagem

Introdução: O cancro pode afectar qualquer pessoa, intromete-se na sua vida pessoa, alterando-a, impondo-se-lhe. Se cuidar é por si só um processo complexo de partilha de afectos, de uma multiplicidade de vertentes que aborda, cuidar em oncologia pela labilidade de fronteiras nas intervenções que encerra, sê-lo-á muito mais. Cuidar do doente com cancro desenvolve-se no seio de fragilidades e de tensões impostas pela doença, pelos tratamentos, pelo sofrimento inerente à doença e a toda a panóplia de alterações que ela transporta. Objetivos: Analisar a experiência de estudantes do 1º ciclo Enfermagem no cuidado à pessoa com cancro. Metodologia: Partindo da questão norteadora qual a experiência dos estudantes de enfermagem no cuidado à pessoa com cancro?, realizou-se estudo descritivo, exploratório, de abordagem qualitativa. A amostra foi constituída por estudantes finalistas com experiência de cuidados a pessoas com cancro. A recolha de dados foi realizada através de narrativas elaboradas pelos estudantes, após consentimento livre e esclarecido. Utilizou-se o software Nvivo7. Procedeu-se a uma análise de conteúdo, a categorização obedeceu ao preconizado por Bardin. Os resultados foram validados pelos sujeitos e a categorização foi posta à consideração de dois peritos investigadores. Resultados: O grupo era constituído por doze estudantes, dos quais dez do género feminino. Todos eram solteiros, com idades compreendidas entre 22 anos e 25 anos, a moda assumiu o valor 22 anos e a média 22,5 anos. Da análise dos dados emergiu a experiência de cuidar em oncologia como área temática. Identificaram-se três categorias, a saber: Características dos cuidados, Aspectos enriquecedores de cuidados, Dificuldades no processo de cuidados. Em cada uma das categorias, nas diferentes narrativas, foram identificadas várias sub-categorias. A categoria características de cuidados surgiu como identificadora da experiência dos cuidados à pessoa com cancro, na qual, disponibilidade para o outro, promover a esperança, contágio emocional, cuidados de qualidade, envolvimento da família, solidariedade dos doentes, escuta, constituíram-se como sub-categorias. Fonte de aprendizagem e realização pessoal foram identificados como aspectos enriquecedores de cuidados. Todavia, o processo de cuidados ao outro não está isento de dificuldades, sendo a impotência e a projecção as dificuldades consideradas pelos estudantes. Conclusão: Caracterizou-se o cenário da experiência de cuidados, na qual se identificaram aspectos enriquecedores e dificuldades, surgindo elementos caracterizadores em que a disponibilidade para o outro e a importância de promover a esperança foram realçadas. Conhecer sentimentos, dificuldades, dos estudantes permite alguma reflexão. Constatou-se que as experiências de cuidados permitiram uma apropriação da realidade. Realça-se a necessidade de tomar medidas que tornem essas experiências significativas, nomeadamente, através da criação de espaços de reflexão sobre os acontecimentos vividos. O incentivo à elaboração de diários de aprendizagem como instrumento de reflexão sobre as práticas pode ser um deles

Elevated temperature, but not decreased pH, impairs reproduction in a temperate fish.

Fish reproductive success is linked to the ability of couples to mate and produce clutches that successfully hatch. Environmental stressors like high temperature and low pH can jeopardize this energetically costly process. In this study, exposure to high temperature and low pH was tested on a marine temperate species, Gobiusculus flavescens, to evaluate effects on reproductive performance. Breeding pairs were assigned to different temperatures (+ 0 °C, + 3 °C relative to in situ temperature) and pH levels (8.0, 7.6), in a cross-factorial design for a 3-month period. Reproduction activity, success, and paternal investment were measured throughout the exposure period. Results show reproduction is impaired by elevated temperature, while low pH had little impact. Breeding pairs under high temperature had 3% to 10% hatching success, up to 30% less eggs and eggs up to 20% smaller. Although paternal investment was not affected by tested parameters, males of breeding pairs exposed to elevated temperature had smaller gonadosomatic indexes, which might indicate a lack of investment in the reproductive process. Overall, results show that elevated temperature, expected more frequently in the near future, as a consequence of global warming, may impair key processes like reproduction in temperate fish, with potential consequences for fitness and population replenishment.Fundação para a Ciência e Tecnologia - FCTinfo:eu-repo/semantics/publishedVersio

THE IMPACT OF INFORMAL CAREGIVERS DURING CANCER PATIENTS’ TRAJECTORY IN ECONOMIC HEALTH OUTCOMES

In the trajectory of the person with cancer, the presence of uncontrolled symptoms are the main and frequent cause of demand for health services, which increases with the advanced stage of the disease. The modernization of health systems advocates the domiciliation of health care, with proximity assistance, where informal caregivers assume a role of relevance. Persons with cancer prefer this typology of service, as well have positive impact in economic health outcomes. However, it is essential to reflect in the shapes of the professional support, health policies and incentives, which are needed with the finality to prevent burnout of the caregiver. As ensure the resources to certify the self-care and quality of life of the person with cancer and informal caregivers

Para uma periodização da pré-história recente do norte de Portugal: da segunda metade do 4º milénio aos finais do 3º milénio AC

Tendo como base novas premissas e novos dados resultantes de projetos de investigação desenvolvidos para a Pré-história Recente, em diferente áreas do Norte de Portugal, as autoras revisitam as diferentes periodizações elaboradas para o período compreendido entre a segunda metade do 4º e os finais do 3º milénios AC (os chamados Neolítico Médio/Final, Calcolítico e Bronze Antigo ou Inicial). A uma ampla escala de análise, reflete-se sobre as bases duma futura periodização conjunta para a região, destacando-se, na longa duração, continuidades e mudanças culturais.Based on new premises and new data resulting from research projects developed for Late Prehistory in different areas of Northern Portugal, the authors review the different periodization’s elaborated for the period between the second half of the fourth and the end of the third millennia BC (the so-called Middle/Late Neolithic, Chalcolithic and Early Bronze Age). A broad scale of analysis reflects on the basis of a future joint periodization for the region, highlighting, in the long run, cultural continuities and changesinfo:eu-repo/semantics/acceptedVersio

The potential role of scavenging flies as mechanical vectors of Lagovirus europaeus/GI.2

The European rabbit (Oryctolagus cuniculus) populations of the Iberian Peninsula have been severely affected by the emergence of the rabbit haemorrhagic disease virus (RHDV) Lagovirus europaeus/GI.2 (RHDV2/b). Bushflies and blowflies (Muscidae and Calliphoridae families, respectively) are important RHDV vectors in Oceania, but their epidemiological role is unknown in the native range of the European rabbit. In this study, scavenging flies were collected between June 2018 and February 2019 in baited traps at one site in southern Portugal, alongside a longitudinal capture-mark-recapture study of a wild European rabbit population, aiming to provide evidence of mechanical transmission of GI.2 by flies. Fly abundance, particularly from Calliphoridae and Muscidae families, peaked in October 2018 and in February 2019. By employing molecular tools, we were able to detect the presence of GI.2 in flies belonging to the families Calliphoridae, Muscidae, Fanniidae and Drosophilidae. The positive samples were detected during an RHD outbreak and absent in samples collected when no evidence of viral circulation in the local rabbit population was found. We were able to sequence a short viral genomic fragment, confirming its identity as RHDV GI.2. The results suggest that scavenging flies may act as mechanical vectors of GI.2 in the native range of the southwestern Iberian subspecies O. cuniculus algirus. Future studies should better assess their potential in the epidemiology of RHD and as a tool for monitoring viral circulation in the field.This study was performed within the scope of project LAGMED (www.lagmed.eu), supported by Fundação para a Ciência e Tecnologia, FCT (PRIMA/0003/2018) and PRIMA programme, an Art.185 initiative supported and funded under Horizon 2020, the European Union’s Framework Programme for Research and Innovation. This work was also co-funded by the European Regional Development Fund (FEDER) and Norte Portugal Regional Operational Programme (NORTE2020), under the PORTUGAL 2020 Partnership Agreement, and by the project NORTE-01-0246-FEDER-000063. The authors also acknowledge FCT research support via the Junior Researcher grant of Ana M. Lopes (CEECIND/01388/2017), the PhD grant of João Vasco Côrte-Real (DFA/BD/4965/2020), the post-doctoral grant of Nuno Santos (SFRH/BPD/116596/2016), the Principal Researcher grant of Pedro J. Esteves (CEECIND/CP1601/CT0005), the Assistant Researcher grant of Joana Abrantes (CEECIND/00078/2017), and projects CIISA UIDB/00276/2020, LA/P/0059/2020-Al4AnimalS and CESAM UID/AMB/50017-POCI-01-0145-FEDER-007638.info:eu-repo/semantics/publishedVersio

Comparing two in-house developed SNP assays for inferring population structure in the honey bee (Apis mellifera L.)

The honey bee, Apis mellifera L., is under pressure globally due to several factors, one of them is the large-scale introduction of foreign queens and/or colonies which act as vectors of pathogens, and also threaten the genetic integrity of native populations. Different molecular tools have been developed to monitor the genetic integrity of the populations. SNPs (Single Nucleotide Polymorphism) have been preferred because are easily transferred between laboratories, have a low genotyping error, provide high-quality data, and are suitable for automation. Here, we compared the genotyping results obtained with two medium-density-SNP assays previously developed. One of assays was designed from 88 whole genomes of Apis mellifera iberiensis and 44 C-lineage individuals (the main ancestry of commercial bees) using fixed SNPs (FST=1) distributed in the 16 honey bee chromosomes. The other assay was designed from variation in immune genes using a discovery panel of 123 whole genomes, representing seven subspecies (A. m. iberiensis, A. m. mellifera, A. m. intermissa, A. m. sahariensis, A. m. ligustica, A. m. carnica, A. m. siciliana and three lineages (A, M and C). All the samples are from the native range of each subspecies and they were taken from inside the hives, placed in absolute ethanol and stored at -20ºC until DNA extraction. The tools were compared using 473 samples from the Azores, which harbour a genetically complex honey bee population. The samples were genotyped using the iPLEX MassARRAY® MALDI-TOF system. The membership proportions of each individual (Qvalue) were calculated using ADMIXTURE considering two genetic groups (K=2), with 10,000 iterations in 20 independent runs. Our results show that both assays provide similar Q-values, with a Pearson’s correlation of 0.89. Only 9.5% of the samples have an absolute Q-value difference > 0.10. The choice of the best SNP assay depends on the subspecies and the aim of the project. While the immune assays can be applied in different subspecies the other assay was specifically designed for A. m. iberiensis. Furthermore, if there is disease data available, the immune assay caninfo:eu-repo/semantics/publishedVersio

Conjugated linoleic acid reduces permeability and fluidity of adipose plasma membranes from obese Zucker rats

NOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications. July 2010; 398 (2): 199-204.Conjugated linoleic acid (CLA) is a dietary fatty acid frequently used as a body fat reducing agent whose effects upon cell membranes and cellular function remain unknown. Obese Zucker rats were fed atherogenic diets containing saturated fats of vegetable or animal origin with or without 1% CLA, as a mixture of cis(c)9,trans(t)11 and t10,c12 isomers. Plasma membrane vesicles obtained from visceral adi- pose tissue were used to assess the effectiveness of dietary fat and CLA membrane incorporation and its outcome on fluidity and permeability to water and glycerol. A significant decrease in adipose membrane fluidity was correlated with the changes observed in permeability, which seem to be caused by the incor- poration of the t10,c12 CLA isomer into membrane phospholipids. These results indicate that CLA supple- mentation in obese Zucker rats fed saturated and cholesterol rich diets reduces the fluidity and permeability of adipose membranes, therefore not supporting CLA as a body fat reducing agent through membrane fluidification in obese fat consumers

Low Serum Levels of DKK2 Predict Incident Low-Impact Fracture in Older Women

There are currently no robust noninvasive markers of fragility fractures. Secreted frizzled related protein-1 (sFRP-1), dickkopf-related protein 1 (DKK1) and DKK2, and sclerostin (SOST) inhibit Wnt signaling and interfere with osteoblast-mediated bone formation. We evaluated associations of serum levels of sFRP-1, DKK1, DKK2, and SOST with incident low-impact fracture and BMD in 828 women aged ≥65 years from EpiDoC, a longitudinal population-based cohort. A structured questionnaire during a baseline clinical appointment assessed prevalent fragility fractures and clinical risk factors (CRFs) for fracture. Blood was collected to measure serum levels of bone turnover markers and Wnt regulators. Lumbar spine and hip BMD were determined by DXA scanning. Follow-up assessment was performed through a phone interview; incident fragility fracture was defined by any new self-reported low-impact fracture. Multivariate Cox proportional hazard models were used to analyze fracture risk adjusted for CRFs and BMD. During a mean follow-up of 2.3 ± 1.0 years, 62 low-impact fractures were sustained in 58 women. A low serum DKK2 level (per 1 SD decrease) was associated with a 1.5-fold increase in fracture risk independently of BMD and CRFs. Women in the two lowest DKK2 quartiles had a fracture incidence rate of 32 per 1000 person-years, whereas women in the two highest quartiles had 14 fragility fractures per 1000 person-years. A high serum sFRP1 level was associated with a 1.6-fold increase in fracture risk adjusted for CRFs, but not independently of BMD. Serum levels of SOST (r = 0.191; p = 0.0025) and DKK1(r = -0.1725; p = 0.011) were correlated with hip BMD, but not with incident fragility fracture. These results indicate that serum DKK2 and sFRP1 may predict low-impact fracture. The low number of incident fractures recorded is a limitation and serum levels of Wnt regulators should be further studied in other populations as potential noninvasive markers of fragility fractures. © 2019 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.publishersversionpublishe

Removal of industrial cutting oil from oil emulsions by polymeric ultra - and microfiltration membranes

The utilization of micro- and ultrafiltration with polymeric membranes for treatment of industrial cutting oil emulsion was investigated. The performance of 14 different membranes with pore sizes in the range of 1-800 nm, representing 8 different materials and varying hydrophobicity, was determined experimentally. Membrane permeances between 1.6 and 939 L m 2h-1bar-1 have been observed for the different samples as well as oil rejections between 3.42% and 99.99%. Membrane pore size and contact angle showed little influence on both values, while an interesting correlation is displayed to the individual membranes capillary pressures. A possible explanation for thisphenomenon is suggested based on the formation of oil films on the membrane surface. From the investigated membranes, the best-suited one for cutting oil treatment was selected and subjected to further experiments. The effect of process temperatures between 22 and 43 °C and of feed oil concentrations between 0 and 20 vol % on the removal performance was determined. The results correspond to the explanation suggested previously