Concatenative speech synthesis: a Framework for Reducing Perceived Distortion when using the TD-PSOLA Algorithm

This thesis presents the design and evaluation of an approach to concatenative speech synthesis using the Titne-Domain Pitch-Synchronous OverLap-Add (I'D-PSOLA) signal processing algorithm. Concatenative synthesis systems make use of pre-recorded speech segments stored in a speech corpus. At synthesis time, the `best' segments available to synthesise the new utterances are chosen from the corpus using a process known as unit selection. During the synthesis process, the pitch and duration of these segments may be modified to generate the desired prosody. The TD-PSOLA algorithm provides an efficient and essentially successful solution to perform these modifications, although some perceptible distortion, in the form of `buzzyness', may be introduced into the speech signal. Despite the popularity of the TD-PSOLA algorithm, little formal research has been undertaken to address this recognised problem of distortion. The approach in the thesis has been developed towards reducing the perceived distortion that is introduced when TD-PSOLA is applied to speech. To investigate the occurrence of this distortion, a psychoacoustic evaluation of the effect of pitch modification using the TD-PSOLA algorithm is presented. Subjective experiments in the form of a set of listening tests were undertaken using word-level stimuli that had been manipulated using TD-PSOLA. The data collected from these experiments were analysed for patterns of co- occurrence or correlations to investigate where this distortion may occur. From this, parameters were identified which may have contributed to increased distortion. These parameters were concerned with the relationship between the spectral content of individual phonemes, the extent of pitch manipulation, and aspects of the original recordings. Based on these results, a framework was designed for use in conjunction with TD-PSOLA to minimise the possible causes of distortion. The framework consisted of a novel speech corpus design, a signal processing distortion measure, and a selection process for especially problematic phonemes. Rather than phonetically balanced, the corpus is balanced to the needs of the signal processing algorithm, containing more of the adversely affected phonemes. The aim is to reduce the potential extent of pitch modification of such segments, and hence produce synthetic speech with less perceptible distortion. The signal processingdistortion measure was developed to allow the prediction of perceptible distortion in pitch-modified speech. Different weightings were estimated for individual phonemes,trained using the experimental data collected during the listening tests.The potential benefit of such a measure for existing unit selection processes in a corpus-based system using TD-PSOLA is illustrated. Finally, the special-case selection process was developed for highly problematic voiced fricative phonemes to minimise the occurrence of perceived distortion in these segments. The success of the framework, in terms of generating synthetic speech with reduced distortion, was evaluated. A listening test showed that the TD-PSOLA balanced speech corpus may be capable of generating pitch-modified synthetic sentences with significantly less distortion than those generated using a typical phonetically balanced corpus. The voiced fricative selection process was also shown to produce pitch-modified versions of these phonemes with less perceived distortion than a standard selection process. The listening test then indicated that the signal processing distortion measure was able to predict the resulting amount of distortion at the sentence-level after the application of TD-PSOLA, suggesting that it may be beneficial to include such a measure in existing unit selection processes. The framework was found to be capable of producing speech with reduced perceptible distortion in certain situations, although the effects seen at the sentence-level were less than those seen in the previous investigative experiments that made use of word-level stimuli. This suggeststhat the effect of the TD-PSOLA algorithm cannot always be easily anticipated due to the highly dynamic nature of speech, and that the reduction of perceptible distortion in TD-PSOLA-modified speech remains a challenge to the speech community

Concatenative speech synthesis : a framework for reducing perceived distortion when using the TD-PSOLA algorithm

This thesis presents the design and evaluation of an approach to concatenative speech synthesis using the Titne-Domain Pitch-Synchronous OverLap-Add (I'D-PSOLA) signal processing algorithm. Concatenative synthesis systems make use of pre-recorded speech segments stored in a speech corpus. At synthesis time, the `best' segments available to synthesise the new utterances are chosen from the corpus using a process known as unit selection. During the synthesis process, the pitch and duration of these segments may be modified to generate the desired prosody. The TD-PSOLA algorithm provides an efficient and essentially successful solution to perform these modifications, although some perceptible distortion, in the form of `buzzyness', may be introduced into the speech signal. Despite the popularity of the TD-PSOLA algorithm, little formal research has been undertaken to address this recognised problem of distortion. The approach in the thesis has been developed towards reducing the perceived distortion that is introduced when TD-PSOLA is applied to speech. To investigate the occurrence of this distortion, a psychoacoustic evaluation of the effect of pitch modification using the TD-PSOLA algorithm is presented. Subjective experiments in the form of a set of listening tests were undertaken using word-level stimuli that had been manipulated using TD-PSOLA. The data collected from these experiments were analysed for patterns of co- occurrence or correlations to investigate where this distortion may occur. From this, parameters were identified which may have contributed to increased distortion. These parameters were concerned with the relationship between the spectral content of individual phonemes, the extent of pitch manipulation, and aspects of the original recordings. Based on these results, a framework was designed for use in conjunction with TD-PSOLA to minimise the possible causes of distortion. The framework consisted of a novel speech corpus design, a signal processing distortion measure, and a selection process for especially problematic phonemes. Rather than phonetically balanced, the corpus is balanced to the needs of the signal processing algorithm, containing more of the adversely affected phonemes. The aim is to reduce the potential extent of pitch modification of such segments, and hence produce synthetic speech with less perceptible distortion. The signal processingdistortion measure was developed to allow the prediction of perceptible distortion in pitch-modified speech. Different weightings were estimated for individual phonemes,trained using the experimental data collected during the listening tests.The potential benefit of such a measure for existing unit selection processes in a corpus-based system using TD-PSOLA is illustrated. Finally, the special-case selection process was developed for highly problematic voiced fricative phonemes to minimise the occurrence of perceived distortion in these segments. The success of the framework, in terms of generating synthetic speech with reduced distortion, was evaluated. A listening test showed that the TD-PSOLA balanced speech corpus may be capable of generating pitch-modified synthetic sentences with significantly less distortion than those generated using a typical phonetically balanced corpus. The voiced fricative selection process was also shown to produce pitch-modified versions of these phonemes with less perceived distortion than a standard selection process. The listening test then indicated that the signal processing distortion measure was able to predict the resulting amount of distortion at the sentence-level after the application of TD-PSOLA, suggesting that it may be beneficial to include such a measure in existing unit selection processes. The framework was found to be capable of producing speech with reduced perceptible distortion in certain situations, although the effects seen at the sentence-level were less than those seen in the previous investigative experiments that made use of word-level stimuli. This suggeststhat the effect of the TD-PSOLA algorithm cannot always be easily anticipated due to the highly dynamic nature of speech, and that the reduction of perceptible distortion in TD-PSOLA-modified speech remains a challenge to the speech community.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Handsearching had best recall but poor efficiency when exporting to a bibliographic tool: case study

This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this recordObjective: To compare the effectiveness and efficiency of methods used to identify and export conference abstracts into a bibliographic management tool. Study design and setting: Case study. The effectiveness and efficiency of methods to identify and export conference abstracts presented at the American Society of Hematology (ASH) conference 2016-2018 for a systematic review were evaluated. A reference standard handsearch of conference proceedings was compared to: 1) contacting Blood (the journal who report ASH proceedings); 2) keyword searching; 3) searching Embase; 4) searching MEDLINE via EndNote; and 5) searching CPCI-S. Effectiveness was determined by the number of abstracts identified compared with the reference standard, while efficiency was a comparison between the resources required to identify and export conference abstracts compared to the reference standard. Results: 604 potentially eligible and 15 confirmed eligible conference abstracts (abstracts included in the review) were identified by the handsearch. Comparator 2 was the only method to identify all abstracts and it was more efficient than the reference standard. Comparators 1, and 3-5 missed a number of eligible abstracts. Conclusion: This study raises potentially concerning questions about searching for conferences’ abstracts by methods other than directly searching the original conference proceedings. Efficiency of exporting would be improved if journals permitted bulk downloads.Takeda Pharmaceutical

Objective assessment of stored blood quality by deep learning

Stored red blood cells (RBCs) are needed for life-saving blood transfusions, but they undergo continuous degradation. RBC storage lesions are often assessed by microscopic examination or biochemical and biophysical assays, which are complex, time-consuming, and destructive to fragile cells. Here we demonstrate the use of label-free imaging flow cytometry and deep learning to characterize RBC lesions. Using brightfield images, a trained neural network achieved 76.7% agreement with experts in classifying seven clinically relevant RBC morphologies associated with storage lesions, comparable to 82.5% agreement between different experts. Given that human observation and classification may not optimally discern RBC quality, we went further and eliminated subjective human annotation in the training step by training a weakly supervised neural network using only storage duration times. The feature space extracted by this network revealed a chronological progression of morphological changes that better predicted blood quality, as measured by physiological hemolytic assay readouts, than the conventional expert-assessed morphology classification system. With further training and clinical testing across multiple sites, protocols, and instruments, deep learning and label-free imaging flow cytometry might be used to routinely and objectively assess RBC storage lesions. This would automate a complex protocol, minimize laboratory sample handling and preparation, and reduce the impact of procedural errors and discrepancies between facilities and blood donors. The chronology-based machine-learning approach may also improve upon humans’ assessment of morphological changes in other biomedically important progressions, such as differentiation and metastasis

An ultra scale-down tool for the predictive design of a filtration procedure for preparation of human cell therapies

With the potential to provide a cure as oppose to a treatment, human cell therapies offer an exciting alternative to biopharmaceuticals. However the challenges associated with whole cell bioprocessing are one of the main issues hindering human cell therapies from becoming commonplace in modern medicine. An ultra scale-down approach to dead end filtration for the recovery of adherent cells for therapy is presented. Initial viable cell yields were low and a number of methods were used in an attempt to improve recovery, with little success. It was possible to recover approximately 85% of the cells but only when the number of cells loaded was low (< 1x106 cells), when a higher number of cells were loaded >1x106 cells, the recoveries were significantly lower. A model is proposed which describes two distinct cell populations; surface cells (TSURF) which reside on the surface of the filter after loading and are almost entirely recovered and filtered cells (TFILT) which enter the filter and are extremely difficult to recover. Results showed that on average 35% ± 11% of filtered cells (TFILT) are recovered regardless of the number of cells loaded. Scanning electron microscopy was used to image the cells residing within the filter. The images showed the cells wrapping around and entangling themselves within the fibres of the filter demonstrating why they are difficult to recover. Finally a method is presented which uses a layer of glass beads on the surface of the filter to prevent the cells coming into contact with filter. By stopping the cells becoming trapped within the filter there was a significant increase in the viable cell recoveries. Using this method it was possible to recover on average 84% ± 6%