Exploring project managers’ perception of stress when working in increasingly complex construction projects

Stress is a recognised feature of the project managers’ life. Projects are becoming more complex with the uncertainty in the system contributing to the lack of control and added stress. This study explores a microcosm of 25 project managers in the South Australian construction industry, their perceived level of uncertainty and stress. The project managers interviewed provide their views on what their key stressors were and the mechanisms they use to manage effectively their stress. The results showed that structural, technical and directional complexity of projects was high, but this did not always convert into more stress for the project managers. The results indicate that stressors like the lack of resources; lack of control and increasing accountability were project managers’ primary concerns. Coping strategies included optimism, applying emotional intelligence and active planning strategies. In addition, they reduced their stress by working with good teams, using theirs and their team's experience, implementing sound systems and processes and effectively researching and gathering information

Altered expression of hypothetical proteins in hippocampus of transgenic mice overexpressing human Cu/Zn-superoxide dismutase 1

BACKGROUND: Cu/Zn-superoxide dismutase 1 (SOD1), encoded on chromosome 21, is a key enzyme in the metabolism of reactive oxygen species (ROS) and pathogenetically relevant for several disease states including Down syndrome (DS; trisomy 21). Systematically studying protein expression in human brain and animal models of DS we decided to carry out "protein hunting" for hypothetical proteins, i.e. proteins that have been predicted based upon nucleic sequences only, in a transgenic mouse model overexpressing human SOD1. RESULTS: We applied a proteomics approach using two-dimensional electrophoresis (2-DE) with in-gel digestion of spots followed by mass spectrometric (matrix-assisted laser desorption/ionization-time of flight) identification and quantification of hypothetical proteins using specific software. Hippocampi of wild type, hemizygous and homozygous SOD1 transgenic mice (SOD1-TGs) were analysed. We identified fourteen hypothetical proteins in mouse hippocampus. Of these, expression levels of 2610008O03Rik protein (Q9D0K2) and 4632432E04Rik protein (Q9D358) were significantly decreased (P < 0.05 and 0.001) and hypothetical protein (Q99KP6) was significantly increased (P < 0.05) in hippocampus of SOD1-TGs as compared with non-transgenic mice. CONCLUSIONS: The biological meaning of aberrant expression of these proteins may be impairment of metabolism, signaling and transcription machinery in SOD1-TGs brain that in turn may help to explain deterioration of these systems in DS brain

Altered Gene Expression in Liver from a Murine Model of Hyperhomocysteinemia

Cystathionine beta-synthase (CBS) deficiency causes severe hyperhomocysteinemia and other signs of homocystinuria syndrome, in particular a premature atherosclerosis with multiple thrombosis. However, the molecular mechanisms by which homocysteine could interfere with normal cell function are poorly understood in a whole organ like the liver, which is central to the catabolism of homocysteine. We used a combination of differential display and cDNA arrays to analyze differential gene expression in association with elevated hepatic homocysteine levels in CBS-deficient mice, a murine model of hyperhomocysteinemia. Expression of several genes was found to be reproducibly abnormal in the livers of heterozygous and homozygous CBS-deficient mice. We report altered expression of genes encoding ribosomal protein S3a and methylthioadenosine phosphorylase, suggesting such cellular growth and proliferation perturbations may occur in homozygous CBS-deficient mice liver. Many up- or down-regulated genes encoded cytochromes P450, evidence of perturbations of the redox potential in heterozygous and homozygous CBS-deficient mice liver. The expression of various genes involved in severe oxidative processes was also abnormal in homozygous CBS-deficient mice liver. Among them, the expression of heme oxygenase 1 gene was increased, concomitant with overexpression of heme oxygenase 1 at the protein level. Commensurate with the difference in hepatic mRNA paraoxonase 1 abundance, the mean hepatic activity of paraoxonase 1, an enzyme that protects low density lipoprotein from oxidation, was 3-fold lower in homozygous CBS-deficient mice. Heterozygous CBS-deficient mice, when fed a hyperhomocysteinemic diet, have also reduced PON1 activity, which demonstrates the effect of hyperhomocysteinemia in the paraoxonase 1 activity

Wolves in the Wolds: Late Capitalism, the English Eerie, and the Wyrd Case of ‘Old Stinker’ the Hull Werewolf

In this article, I depart from the earlier opinions of Emily Gerard, Sabine Baring-Gould, and others, who explained the disappearance of the werewolf in folklore as following the extinction of the wolf. I argue instead that British literature is distinctive in representing a history of werewolf sightings in places in Britain where there were once wolves. I draw on the idea of absence, manifestations of the English eerie, and the turbulence of England in the era of late capitalism to illuminate my analysis of the representation of contemporary werewolf sightingsPeer reviewe

Proximity to overhead power lines and childhood leukaemia: an international pooled analysis

© 2018, Cancer Research UK. Background: Although studies have consistently found an association between childhood leukaemia risk and magnetic fields, the associations between childhood leukaemia and distance to overhead power lines have been inconsistent. We pooled data from multiple studies to assess the association with distance and evaluate whether it is due to magnetic fields or other factors associated with distance from lines. Methods: We present a pooled analysis combining individual-level data (29,049 cases and 68,231 controls) from 11 record-based studies. Results: There was no material association between childhood leukaemia and distance to nearest overhead power line of any voltage. Among children living < 50 m from 200 + kV power lines, the adjusted odds ratio for childhood leukaemia was 1.33 (95% CI: 0.92–1.93). The odds ratio was higher among children diagnosed before age 5 years. There was no association with calculated magnetic fields. Odds ratios remained unchanged with adjustment for potential confounders. Conclusions: In this first comprehensive pooled analysis of childhood leukaemia and distance to power lines, we found a small and imprecise risk for residences < 50 m of 200 + kV lines that was not explained by high magnetic fields. Reasons for the increased risk, found in this and many other studies, remains to be elucidated

Early thymic T cell development in young transgenic mice overexpressing human Cu/Zn superoxide dismutase, a model of Down syndrome.: Early thymic development in SOD1 transgenic mice.

Previous studies have shown that transgenic mice overexpressing Cu/Zn superoxide dismutase, a model of Down syndrome, exhibit premature thymic involution. We have performed a flow cytometry analysis of the developing thymus in these homozygous transgenic mice (hSOD1/hSOD1: Tg-SOD). Longitudinal follow-up analysis from day 3 to day 280 showed an early thymic development in Tg-SOD mice compared with controls. This early thymic development was associated with an increased migration of mature T cells to peripheral lymphoid organs. BrdU labeling showed no difference between Tg-SOD and control mice, confirming that the greater number of peripheral T cells in Tg-SOD mice was not due to extensive proliferation of these cells but rather to a greater pool of emigrant T cells in Tg-SOD

Contribution à l'étude des mécanismes cellulaires et moléculaires induits par l'hyperhomocysteinemie

PARIS7-Bibliothèque centrale (751132105) / SudocSudocFranceF

When the Storm Comes

This senior honors thesis, titled "When the Storm Comes," is a book of poems that details both the struggles and joys of growing up in a Southern community. Though there are many storms that exist throughout this work, the speaker finds ways to navigate these storms and turn these lived experiences into powerful memories and lessons.Bachelor of Art

Epidemiological analysis of skin problems in down syndrome peuple and study o the skin of transgenic mice for human gene encoding the sod-1

La trisomie 21 (T21) est un syndrome complexe affectant principalement le développement neuronal mais aussi les systèmes auditif, oculaire, endocrinien, immunitaire et tégumentaire. Les personnes atteintes de T21 présentent de plus un tableau clinique de vieillissement précoce. L'augmentation du stress oxydant due à la surexpression de la SuperOxyde Dismutase 1 gène du chromosome 21 peut en partie expliquer ce vieillissement précoce. Cette thèse, à l'aide de souris transgéniques, s'inscrit dans l'étude des anomalies cutanées que présentent les patients atteints de T21 et qui ont été peu étudiées à ce jour. Citons parmi ces pathologies l'alopécie areata, les dermatoses (syringome), une xérose ainsi que les infections fongiques et bactériennes très fréquentes chez ces patients. Mon travail de thèse s'est organisé en deux parties : Une étude épidémiologique et une étude expérimentale. Pour l'étude épidémiologique, nous avons rédigé un questionnaire qui a été envoyé à 500 familles, 61 réponses ont pu être analysées. L'étude expérimentale comprend 4 chapitres : 1-Caractérisation de la peau de souris transgéniques de 4 mois. 2- Caractérisation en fonction de l'âge. 3- Effet de la surexpression de la SOD-1 sur le protéasome 20S dans la peau et d'autres organes en fonction de l'âge. 4- Etude de la capacité de ré-épithélialisation cutanée chez des souriceaux TghSOD-1. L'ensemble des résultats obtenus permet de conclure que la surexpression de la SOD induit une altération de la peau, un stress oxydant accru et une diminution des capacités du protéasome à assurer son rôle physiologique.PARIS7-Bibliothèque centrale (751132105) / SudocPARIS-BIUP (751062107) / SudocSudocFranceF

Down syndrome and aging (alaysis of consequences at transcriptional and traductional level of SOD-1 and APP genes overexpression)

LA TRISOMIE EST LA PLUS FREQUENTE DES ANOMALIES CHROMOSOMIQUES. CETTE MALADIE EST DUE DANS 90% DES CAS A UN CHROMOSOME 21 SURNUMERAIRE. LE TABLEAU CLINIQUE EST COMPLEXE ET VARIABLE. LES PERSONNES ATTEINTES DE TRISOMIE 21 PRESENTENT DES SIGNES DE VIEILLISSEMENT PREMATURE TELS QU'UN BLANCHIMENT DES CHEVEUX, UNE CATARACTE ET UNE INVOLUTION PRECOCE DU THYMUS. L'UN DES FAITS MARQUANT EST LA PRESENCE DE SIGNES NEUROANATOMOATHOLOGIQUES DE LA MALADIE D'ALZHEIMER. UNE CORRELATION ENTRE TRISOMIE 21 ET MALADIE D'ALZHEIMER A ETE SOUVENT EVOQUEE SANS QUE DES DONNEES PRECISES SOIENT DISPONIBLES SUR LE PLAN DE LA PATHOLOGIE. LA PRESENCE DU CHROMOSOME SURNUMERAIRE 21 EST A L'ORIGINE DE LA SUREXPRESSION DES GENES CODANT LA SOD-1 ET L'APP, MAIS DE NOMBREUSES INCONNUES DEMEURENT SUR LES CONSEQUENCES DE CETTE SUREXPRESSION DANS LE DEVELOPPEMENT DE LA PATHOLOGIE. LES PATIENTS ATTEINTS DE TRISOMIE 21 ONT UN STRESS OXYDANT ACCRU DONT L'ORIGINE POURRAIT ETRE DUE A LA SUREXPRESSION DE LA SOD-1 ET TOUT COMME LES PATIENTS ATTEINTS D'ALZHEIMER, ILS PRESENTENT UN METABOLISME ANORMAL DE LA PROTEINE APP CONDUISANT A UNE AUGMENTATION DE PEPTIDES AB CIRCULANTS ET A LA FORMATION DE PLAQUES SENILES. AFIN DE MIEUX COMPRENDRE LES MECANISMES MIS EN JEU DANS LE VEILLISSEMENT PRECOCE DES PATIENTS ATTEINTS DE TRISOMIE 21, NOUS AVONS ORIENTE NOTRE TRAVAIL VERS L'ETUDE DES ACTIVITES DU PROTEASOME, L'OXYDATION DES PROTEINES ET LES MECANISMES D'APOPTOSE DANS LE SYSTEME NERVEUX CENTRAL ET LE THYMUS, DEUX ORGANES PARTICULIEREMENT TOUCHES PAR LE VIEILLISSEMENT PRECOCE CHEZ LES PATIENTS ATTEINTS DE TRISOMIE 21. POUR CELA, NOUS AVONS UTILISE DES MODELES MURINS SIMPLE ET DOUBLE TRANSGENIQUES POUR LES GENES HUMAINS SOD-1 (HSOD-1) ET APP (HAPP). LA MODELISATION DE LA TRISOMIE 21 PROPOSEE DANS CE TRAVAIL DEVRAIT CONSTITUER UN OUTIL POUR MIEUX COMPRENDRE LES EVENEMENTS PRECOCES IMPLIQUES DANS LE VIEILLISSEMENT NORMAL ET PATHOLOGIQUE POUR ENVISAGER DES THERAPEUTIQUES.PARIS7-Bibliothèque centrale (751132105) / SudocSudocFranceF