Chemicophisical characteristics of preferential host of Grapholita molesta : buds and fruits of peach tree, Mendoza, Argentina

Grapholita molesta (Busck) es una plaga que tiene como principal hospedero el duraznero donde produce daños en brotes y frutos. El estudio de características físicoquímicas, en dichos órganos vegetales (pH, sólidos solubles, acidez y contenido de vitamina C) puede brindar información sobre los hábitos alimentarios de dicha plaga. Los objetivos del trabajo fueron: 1) Determinar características físico-químicas como pH, sólidos solubles, acidez y vitamina C en brotes y frutos de duraznero (cvs. Bowen y Fortuna) durante el período vegetativo y 2) Establecer la influencia de las características investigadas en relación con los daños producidos por la especie, en el mismo monte frutal, hasta la cosecha de los frutos. El máximo daño, en brotes y en frutos, se observó hacia el final de la evaluación, previo a la cosecha. Teniendo en cuenta las determinaciones analíticas de brotes y frutos y la evaluación de sus daños, la plaga puede alcanzar un desarrollo óptimo cuando el pH está comprendido entre 3.76 y 5.93, el contenido de sólidos solubles entre 6 y 12 %, la acidez oscila entre 0.17 a 0.39 g%g de ácido cítrico y el contenido de vitamina C está comprendido entre 7.05 y 61.9 mg%g.Peach tree is the main host of Grapholita molesta (Busck). This pest produces damages in buds and fruits. The study of chemicophysical characteristics (pH, soluble solids, acidity and vitamin C content) in these plant organs can offer information on feeding habits of this pest. This work was made in order: 1) To determine chemicophysical characteristics like pH, soluble solids, acidity and vitamin C in buds and fruits of peach tree (cvs. Bowen and Fortuna) during vegetative period and 2) To establish the influence of the investigated characteristics in relation to damages produced by the species, from fruit orchard to harvest. The greatest damage in buds and fruits was observed in the end of evaluation, previous to harvest. Considering analytical determinations of buds and fruits and damage evaluation, pest can reach the best development when pH was included between 3.76 and 5.93, soluble solids content between 6 and 12 %, acidity between 0.17 to 0.39 g%g of citric acid and vitamin C content between 7.05 and 61.9 mg%g.Fil: Llanos, Lorena Vanina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Departamento de Ciencias BiológicasFil: Marín, María Susana. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Departamento de Ciencias Biológica

The Triassic-Jurassic boundary in the Andes of Argentina

The Arroyo Malo Formation at Alumbre Creek, on the northern bank of the Atuel River, west central Argentina, comprises a c. 300 m thick continuous marine succession across the Triassic-Jurassic System boundary, consisting of massive and laminated pelites indicative of a slope depositional environment. Late Triassic invertebrates, including ammonoids, nautiloids, bivalves, gastropods, brachiopods and corals are restricted to the lower 150 m. Beds between 125-135 m from the bottom yield Choristoceras cf. marshi Hauer, a species found in the Marshi/Crickmayi Zone of Europe and North America, together with loose fragments of Psiloceras cf. pressum Hillebrandt, coeval with the lower to middle part of the Hettangian Planorbis Zone. About 80 m higher are beds yielding Psiloceras cf. rectocostatum Hillebrandt, a species that gives name to an Andean biozone partially coeval with the Johnstoni and Plicatulum Subzones, upper Planorbis Zone. Other fossils recorded in the Rhaetian strata of this section are foraminifers, ostracods and plant remains identified as Zuberia cf. zuberi (Szaj.) Freng. and Clathropteris sp. The section was also sampled for conodonts and radiolarians, thus far with negative results. A palaeomagnetic study is underway.La Formazione Arroyo Malo ad Alumbre creek, sulla sponda settentrionale del fiume Atuel, Argentina centro-occidentale, comprende una successione marina continua spessa circa 300 m attraverso i! limite Triassico-Giurassico, ed e costituita da peliti massive e laminate indicative di un ambiente deposizionale di scarpata. Gli inverttfjrati del Ttiassico superiore, che includono ammonoidi, nautiloidi, bivalvi, gasteropodi, brachiopodi e coralli sono limitati ai primi 150 m. Gli strati fra i 125-135 m dalla base hanno dato Choristoceras cf. marshi Hauer, una specie trovata nella Zona a Marshi/Crickmayi di Europa e Nord America, insieme con frammenti sparsi di Psiloceras cf. pressum Hillebrandt, coeva con la parte mediana della Zona a Planorbis dell'Hettangiano. Circa. 80 m piu in alto ci sono strati contenenti Psiloceras cf. rectocostatum Hlllebrandt, una specie che da i! no me ad una biozona andina parzialmente coeva con le Sottózone a Johnstoni e Plicatulum, Zona a Planorbis superiore. Altri fossili documentati negli strati del Retico di questa sezione sono foraminiferi, ostracodi e resti di piante identificati come Zuberia cf. zuberi (Szaj.) Freng. e Clathropteris sp. La sezione e stata anche campionata per quanto riguarda conodonti e radiolari, finora con risultati negativi. Uno studio paleomagnetico e in corso.Fil: Riccardi, Alberto Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento de Paleontología Invertebrados; ArgentinaFil: Damborenea, Susana Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento de Paleontología Invertebrados; ArgentinaFil: Manceñido, Miguel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento de Paleontología Invertebrados; ArgentinaFil: Iglesia Llanos, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Geología. Instituto de Geofísica "Daniel Valencio"; Argentin

Calidad del servicio y satisfacción del usuario en la dispensación de productos farmacéuticos en tiempos COVID-19. Hospital de Virú

Con el COVID-19, la relación entre la calidad de los servicios farmacéuticos y la satisfacción del usuario a nivel de los establecimientos de salud, se ha convertido en uno de los elementos de la crisis sanitaria mundial más observado, sobre todo por la forma en que se dimensiona sus implicancias sobre la atención de la salud de las personas. Siguiendo esta línea referencial se planteó como objetivo de investigación determinar la relación entre la calidad del servicio de dispensación de productos farmacéuticos con la satisfacción del usuario en tiempos COVID-19, en el Hospital de Virú, 2020. Para ello se desarrolló una investigación descriptiva correlacional de diseño transversal involucrando a 134 usuarios, quienes aplicaron para los cuestionarios PECASSUS (MINPS-Colombia), sobre la calidad percibida por el usuario y, el cuestionario SERVQUAL (MINSA, 2011), sobre la satisfacción del usuario con el servicio recibido. Se encontró que, en tiempos COVID-19, la calidad del servicio es predominantemente Media (50,7%); el usuario se encuentra Poco Satisfecho/a tanto a nivel general (59,7%), como con la calidad de la fiabilidad (56,0%), responsabilidad (58,2%), seguridad (54,5%), empatía (51,5%) y tangibilidad (54,5%). Se concluye que, en tiempos COVID-19, existe una relación muy significativa entre la calidad del servicio con la satisfacción del usuario a nivel general (SpR-0,664; p<0,001) y con las dimensiones de fiabilidad (SpR-0,636; p<0,001), responsabilidad (SpR-0,586; p<0,001), seguridad (SpR-0,589; p<0,001), empatía (SpR-0,586; p<0,001) y tangibilidad (SpR-0,585; p<0,001), en el Hospital de Virú, 2020

Seasonal diversity and safety evaluation of enterococci population from goat milk in a farm

International audienceAbstractThe presence and genetic diversity of enterococci in raw goat milk sampled in a farm during consecutive seasons were evaluated. Representative strains were screened for virulence determinants, susceptibility to antibiotics and the presence of genes involved in biogenic amine production. Counts of enterococci ranged between 2.80 × 101 and 2.50 × 103 cfu.mL–1 with summer samples showing the highest counts and winter samples the lowest. A total of 695 enterococci were isolated and genotyped. One hundred and thirty-three representative isolates from genotypes obtained in randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis were identified as belonging to Enterococcus faecium (62.9%), Enterococcus faecalis (28.2%) and Enterococcus hirae (8.9%) species. A higher number of genotypes was observed in the summer and spring samples. The occurrence of antibiotic resistance and virulence genes was strain dependent, although the presence of genes did not always imply resistance, as occurred for vancomycin. All three species showed a high percentage of resistance to tetracycline. Strains from the warmer seasons (spring, summer and autumn) were resistant to a higher number of antibiotics and harboured a higher number of antibiotic resistance and virulence genes. All the strains produced tyramine, while only one E. hirae strain from a spring sample produced putrescine. The feed regime of goats during the warm seasons was the main difference between samples and thus, it could be proposed that the feed is responsible for the differences in the results between seasons

Prevalencia de enfermedades crónicas en adultos mayores atendidos en el área de consulta externa del Hospital IESS Milagro

El objetivo de este estudio fue determinar la prevalencia de enfermedades crónicas en adultos mayores atendidos en el área de consulta externa del Hospital IESS Milagro, Ecuador. Se realizó un estudio cuantitativo no experimental, además se usó un estudio de tipo transversal con alcance descriptivo, en el cual se incluyeron todos los adultos mayores atendidos en el área de consulta externa del Hospital IESS Milagro durante el año 2021. Los datos fueron recolectados mediante la revisión de la historia clínica de los pacientes y a través de una encuesta sobre factores sociodemográficos y clínicos. Se determinó que la prevalencia de enfermedades crónicas en adultos mayores atendidos en el área de consulta externa del Hospital IESS Milagro fue del 48.57%. Las enfermedades crónicas más prevalentes fueron la hipertensión arterial (27.13%) y la diabetes mellitus (8.40%). Se concluye que la prevalencia de enfermedades crónicas en adultos mayores atendidos en el área de consulta externa del Hospital IESS Milagro es alta. Se recomienda realizar estudios más amplios para determinar las causas de esta alta prevalencia y para implementar medidas preventivas y de control adecuadas.The objective of this study was to determine the prevalence of chronic diseases in older adults treated in the outpatient area of ​​the IESS Milagro Hospital, Ecuador. A non-experimental quantitative study was carried out, in addition, a cross-sectional study with a descriptive scope was used. in which all the older adults treated in the outpatient area of ​​the IESS Milagro Hospital during the year 2021 were included. The data were collected by reviewing the clinical history of the patients and through a survey on sociodemographic and clinical factors. It was determined that the prevalence of chronic diseases in older adults treated in the outpatient area of ​​the IESS Milagro Hospital was 48.57%. The most prevalent chronic diseases were arterial hypertension (27.13%) and diabetes mellitus (8.40%). It is concluded that the prevalence of chronic diseases in older adults treated in the outpatient area of ​​the IESS Milagro Hospital is high. Larger studies are recommended to determine the causes of this high prevalence and to implement adequate preventive and control measures. &nbsp

The Triassic-Jurassic boundary in the Andes of Argentina

The Arroyo Malo Formation at Alumbre Creek, on the northern bank of the Atuel River, west central Argentina, comprises a c. 300 m thick continuous marine succession across the Triassic-Jurassic System boundary, consisting of massive and laminated pelites indicative of a slope depositional environment. Late Triassic invertebrates, including ammonoids, nautiloids, bivalves, gastropods, brachiopods and corals are restricted to the lower 150 m. Beds between 125-135 m from the bottom yield Choristoceras cf. marshi Hauer, a species found in the Marshi/Crickmayi Zone of Europe and North America, together with loose fragments of Psiloceras cf. pressum Hillebrandt, coeval with the lower to middle part of the Hettangian Planorbis Zone. About 80 m higher are beds yielding Psiloceras cf. rectocostatum Hillebrandt, a species that gives name to an Andean biozone partially coeval with the Johnstoni and Plicatulum Subzones, upper Planorbis Zone. Other fossils recorded in the Rhaetian strata of this section are foraminifers, ostracods and plant remains identified as Zuberia cf. zuberi (Szaj.) Freng. and Clathropteris sp. The section was also sampled for conodonts and radiolarians, thus far with negative results. A palaeomagnetic study is underway.Facultad de Ciencias Naturales y Muse

Factors that influence the pregnancy of adolescents

La relación sexual a una edad temprana y la presencia de un embarazo en adolescentes se ha incrementado significativamente en los últimos años. Esto se asocia con factores sociodemográficos, económicos y culturales. El objetivo de este trabajo de investigación fue determinar los factores que influyen en el embarazo de las adolescentes que acudieron al área Materno Infantil del hospital IESS en un periodo de un año.&nbsp; El diseño de la investigación fue cuantitativo y descriptivo.&nbsp; Los datos se tomaron con consentimiento de 20 pacientes con edades entre 10 a 19 años. Obteniendo como resultado mayor frecuencia de adolescentes embarazadas entre 17 a 19 años de edad, las cuales se encuentran cursando la secundaria, su ingreso económico depende de sus padres, el 40% sufren maltrato tanto físico como psicológico. Las adolescentes con un&nbsp; rango entre 14 y 19 años han decidido tener relaciones sexuales por diversión, placero curiosidad, solo&nbsp; el 60% recibió información sobre la sexualidad y conoce los métodos de planificación familiar. En conclusión, para el mejoramiento de la salud pública, se deben generar acciones preventivas hacia la población en general, con el fin de contribuir a mejorar la calidad de vida de las adolescentes.The sexual relationship at an early age and the presence of a pregnancy in adolescents has increased significantly in recent years. This is associated with socio-demographic, economic and cultural factors. The objective of this research was to determine the factors that influence the pregnancy of adolescents who attended the maternal child health area of the IESS hospital in a period of one year. The research design was quantitative and descriptive. The data were taken with the consent of 20 patients with ages between 10 to19 years old. A higher frequency of pregnant teenagers between 17 to 19 years of age was obtained as a result; who are in high school, their income depends on their parents, and 40% suffer both physical and psychological abuse. Adolescents with a range between 14 and 19 years have decided to have sex for fun, curiosity, and only 60% received information about sexuality and knows family planning methods. In conclusion, for the improvement of public health, preventive actions should be generated towards the population in general, in order to help improve the quality of life of adolescents

Stabilization of p21 by mTORC1/4E-BP1 predicts clinical outcome of head and neck cancers

The levels, regulation and prognostic value of p21 in head and neck squamous cell carcinomas (HNSCC) has been puzzling for years. Here, we report a new mechanism of regulation of p21 by the mTORC1/4E-BP1 pathway. We find that non-phosphorylated 4E-BP1 interacts with p21 and induces its degradation. Accordingly, hyper-activation of mTORC1 results in phosphorylation of 4E-BP1 and stabilization of p21. In HNSCC, p21 levels strongly correlate with mTORC1 activity but not with p53 status. Finally, clinical data indicate that HNSCC patients with p21 and phospho-S6-double-positive tumours present a better disease-specific survival. We conclude that over-activation of the mTORC1/4E-BP1/p21 pathway is a frequent and clinically relevant alteration in HNSCC.We are grateful to Reidar Grenman, Silvio Gutkind, Nahum Sonenberg, Gordon Peters, David Sabatini and Mariano Barbacid for sharing critical reagents. We also thank Aurora Astudillo, Aitana Vallina, Laura Alonso-Dura ́n and Eva Allonca for excellent technical assistance. Work in the laboratory of M.S. is funded by the CNIO and by grants from the Spanish Ministry of Economy (SAF) co-funded by the European Regional Development Fund, the European Research Council (ERC Advanced Grant), the Regional Government of Madrid co-funded by the European Social Fund, the Botin Foundation and BancoSantander (Santander Universities Global Division), the Ramon Areces Foundation an the AXA Foundation. Work in the laboratory of J.M.G.-P. and J.P.R. was supported bygrants from Plan Nacional de DþI 2013–2016 ISCIII (CP13/00013 andPI13/00259),RD12/0036/0015 of Red Tematica de Investigacio ́n Cooperativa en Cancer (RTICC), Spain and the FEDER Funding Program from the European UnionS

Are the Portable Air Cleaners (PAC) really effective to terminate airborne SARS-CoV-2?

The transmission of SARS-CoV-2 virus through aerosols has become an outstanding issue,where plenty of spread aspects are being analyzed. Portable Air Cleaners (PAC) with high-efficiency particulate air (HEPA) filters have been discussed as an adjunctive means for indoor environments coronavirus decontamination. This study evaluates, first, the air and surfaces SARS-COV-2 RNA contamination due to positive patients in households, and second, the efficiency of a PAC with HEPA filter to eliminate virus. A total of 29 air and surface sampleswere collected inside 9 households, by using an air portable collector with gelatin filters and swabs. SARS-CoV-2 RNA detection was performed using real-time reverse transcription polymerase chain reaction (RT-PCR). Overall, all the air samples collected before using PAC and 75% of swab samples were positive for SARS-CoV-2. After the PAC usage, all samples except one were negative, displaying a 80% device effectiveness. Portable HEPA cleaners usage allowed the removal of SARS CoV-2 and, therefore, they could be recommended for places with inadequate ventilation, considering the limitations and functionality of the devic

PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks

DNA interstrand crosslinks (ICLs) induced by endogenous aldehydes or chemotherapeutic agents interfere with essential processes such as replication and transcription. ICL recognition and repair by the Fanconi Anemia pathway require the formation of an X-shaped DNA structure that may arise from convergence of two replication forks at the crosslink or traversing of the lesion by a single replication fork. Here, we report that ICL traverse strictly requires DNA repriming events downstream of the lesion, which are carried out by PrimPol, the second primase-polymerase identified in mammalian cells after Polα/Primase. The recruitment of PrimPol to the vicinity of ICLs depends on its interaction with RPA, but not on FANCM translocase or the BLM/TOP3A/RMI1-2 (BTR) complex that also participate in ICL traverse. Genetic ablation of PRIMPOL makes cells more dependent on the fork convergence mechanism to initiate ICL repair, and PRIMPOL KO cells and mice display hypersensitivity to ICL-inducing drugs. These results open the possibility of targeting PrimPol activity to enhance the efficacy of chemotherapy based on DNA crosslinking agents.Spanish Ministry of Science, Innovation and Universities (grants BFU2016-80402R and BFU2019-106707-RB to JM and PGC2018.093576-B-C21 to LB, co-sponsored by EU ERDF funds), the Swiss National Science Foundation (grant 31003A_169959 to ML), and an ERC Consolidator Grant (617102 to ML