Poziom satysfakcji z pracy pracowników administracji publicznej na przykładzie Urzędu X

This paper aims to focus on the importance of job satisfaction of public sector employees. Firstly, the theoretical background on job satisfaction in the context of New Public Management is presented. Next the results of a survey research on job satisfaction in one of the public sector organization are explored. On the basis of the analysis, the authors show that despite the fact that workers are not satisfied with some of the factors in their work place, overall job satisfaction in the examined organization is highCelem niniejszego artykułu jest pokazanie znaczenia satysfakcji z pracy pracowników administracji publicznej, dla właściwej realizacji celów stojących przed tego typu organizacjami. Zaprezentowane w artykule rozważania teoretyczne pokazują zagadnienie satysfakcji z pracy, determinant i czynników kształtujących zadowolenie z pracy w kontekście rozwiązań postulowanych w ramach Nowego Zarządzania Publicznego (New Public Management). W drugiej części artykułu zaprezentowano wyniki badań empirycznych satysfakcji z pracy przeprowadzonych wśród pracowników urzędu administracji państwowej. Rozbieżności, jakie uzyskano w trakcie analizy wyników badań pokazują, że pomiędzy czynnikami na poziomie deklaratywnym a tymi, które występują w rzeczywistości nie ma konsekwencji. Pomimo, że pracownicy mają duże zastrzeżenia, co do występowania poszczególnych czynników satysfakcji z pracy, czują zadowolenie z pracy w Urzędzi

Macrophage-specific RAM11 monoclonal antibody cross-reacts with basal cells of stratified squamous epithelia

RAM11 is a mouse monoclonal anti-rabbit macrophage antibody recognizing connective tissue and vascular (atheromatous tissue) macrophages. This study demonstrates a cross-reaction of RAM11 with an unknown antigen in rabbit normal epithelial cells. Formalin-fixed, paraffin sections of the New Zealand White rabbit normal skin, oral mucosa, esophagus, small intestine and lung were immunostained with RAM11 antibody followed by goat anti-mouse Cy-3-conjugated antiglobulin. RAM11-positive immunofluorescence was observed in basal layer cells of stratified squamous epithelia (skin, oral mucosa, esophagus). No RAM11 immunostaining was found in any cells of simple (intestinal, bronchial) epithelia. These findings show that basal cells of stratified squamous keratinized and non-keratinized epithelia of the rabbit express an antigenic epitope which is common with that of macrophage antigen recognized by RAM11 monoclonal antibody

WYKORZYSTANIE PLATFORMY CDE WE WSPÓŁPRACY ZESPOŁOWEJ W BIM

In project processes, group collaboration and project documentation management are important aspects. In order for the cooperation of all project participants to be effective, it should be based first and foremost on adequate and effective communication. All project participants should use such solutions so that they can exchange, manage and combine information quickly and efficiently throughout the entire investment process, thus providing a complete picture of the situation. To this end, it is necessary to develop a catalogue of good practices supported by a variety of examples, as well as rules for group cooperation when using a CDE-type solution. The aim of this article was to show the advantages and benefits as well as the disadvantages and limitations in group collaboration when working on a single BIM model.W procesach projektowych ważny aspekt stanowi współpraca grupowa oraz zarządzanie dokumentacją projektową. Aby współpraca wszystkich uczestników projektu była efektywna, powinna opierać się przede wszystkim na odpowiedniej i efektywnej komunikacji. Wszyscy uczestnicy projektu powinni używać takich rozwiązań, aby przez cały proces inwestycyjny mogli szybko i sprawnie wymieniać się informacjami, zarządzać nimi i łączyć je ze sobą, dostarczając w ten sposób kompletny obraz danej sytuacji. W tym celu konieczne jest opracowanie katalogu dobrych praktyk podpartych różnorodnymi przykładami oraz zasad współpracy grupowej przy stosowaniu rozwiązań typu CDE. Celem artykułu było pokazanie zalet i korzyści oraz wad i ograniczeń we współpracy grupowej podczas pracy na jednym modelu BIM

Macrophage-specific RAM11 monoclonal antibody cross-reacts with basal cells of stratified squamous epithelia.

RAM11 is a mouse monoclonal anti-rabbit macrophage antibody recognizing connective tissue and vascular (atheromatous tissue) macrophages. This study demonstrates a cross-reaction of RAM11 with an unknown antigen in rabbit normal epithelial cells. Formalin-fixed, paraffin sections of the New Zealand White rabbit normal skin, oral mucosa, esophagus, small intestine and lung were immunostained with RAM11 antibody followed by goat anti-mouse Cy-3-conjugated antiglobulin. RAM11-positive immunofluorescence was observed in basal layer cells of stratified squamous epithelia (skin, oral mucosa, esophagus). No RAM11 immunostaining was found in any cells of simple (intestinal, bronchial) epithelia. These findings show that basal cells of stratified squamous keratinized and non-keratinized epithelia of the rabbit express an antigenic epitope which is common with that of macrophage antigen recognized by RAM11 monoclonal antibody

Expression of basal cell marker revealed by RAM11 antibody during epithelial regeneration in rabbits.

RAM11 is a mouse monoclonal anti-rabbit macrophage antibody recognizing connective tissue and vascular macrophages. Our previous report showed that RAM11 reacted with basal cells of stratified squamous epithelia of rabbit skin, oral mucosa and esophagus. The aim of the present study was to follow the appearance of RAM11 immunoreactivity in basal cells of regenerating oral epithelium in rabbits. No RAM11 immunostaining was observed in the regenerating epithelium examined on days 1 and 3 of wound healing. A weak immunofluorescence first appeared on day 7 in single basal cells and 32% of RAM11- positive basal cells were observed on day 14. These findings indicate that expression of the antigen recognized by RAM11 antibody is a transient event in the differentiation of oral keratinocytes which not always occurs during epithelial repair, although it is a constant feature of epithelial turnover in mature epithelium. Therefore this antigen can be regarded as basal cell marker only in mature stratified squamous epithelia

Missense-depleted regions in population exomes implicate ras superfamily nucleotide-binding protein alteration in patients with brain malformation.

Genomic sequence interpretation can miss clinically relevant missense variants for several reasons. Rare missense variants are numerous in the exome and difficult to prioritise. Affected genes may also not have existing disease association. To improve variant prioritisation, we leverage population exome data to identify intragenic missense-depleted regions (MDRs) genome-wide that may be important in disease. We then use missense depletion analyses to help prioritise undiagnosed disease exome variants. We demonstrate application of this strategy to identify a novel gene association for human brain malformation. We identified de novo missense variants that affect the GDP/GTP-binding site of ARF1 in three unrelated patients. Corresponding functional analysis suggests ARF1 GDP/GTP-activation is affected by the specific missense mutations associated with heterotopia. These findings expand the genetic pathway underpinning neurologic disease that classically includes FLNA. ARF1 along with ARFGEF2 add further evidence implicating ARF/GEFs in the brain. Using functional ontology, top MDR-containing genes were highly enriched for nucleotide-binding function, suggesting these may be candidates for human disease. Routine consideration of MDR in the interpretation of exome data for rare diseases may help identify strong genetic factors for many severe conditions, infertility/reduction in reproductive capability, and embryonic conditions contributing to preterm loss

Связь между вознаграждением и удовлетворением от работы: исследование на примере сотрудников государственного управления в Померании в Польше

Remuneration is considered as one of the key factors encouraging people to work. However, its significance for the employee and its effect on job satisfaction remains unclear. The aim of this paper was to determine how remuneration affects job satisfaction among public administration employees from one of the East European countries. The study was conducted by carrying out a survey among employees of 10 public administration offices of the Pomerania Region in Poland. The analysis of the results allowed to draw conclusions on the significance of remuneration in the context of other satisfaction factors, and to assess how relative assessment of the amount of remuneration can affect the relation between remuneration and overall job satisfaction.Винагорода вважається одним з ключових факторів, що заохочують людей до праці. Тим не менш, її значення для працівника і вплив на задоволеність роботою, залишається неясним. Метою даної роботи було визначення того, як винагорода впливає на задоволеність роботою серед співробітників державного управління в одній з країн Східної Європи. Дослідження було здійснено шляхом проведення опитування серед співробітників 10 відділень державного управління регіону Померанія в Польщі. Аналіз результатів дозволив зробити висновки про значення винагороди в контексті інших факторів задоволеності, а також оцінити, як порівняльна оцінка суми винагороди може вплинути на взаємозв’язок між винагородою і загальною задоволеністю роботою.Вознаграждение считается одним из ключевых факторов, поощряющих людей работать. Тем не менее, его значение для работника и его влияние на удовлетворенность работой, остается неясным. Целью данной работы было определение того, как вознаграждение влияет на удовлетворенность работой среди сотрудников государственного управления в одной из стран Восточной Европы. Исследование было осуществлено путем проведения опроса среди сотрудников 10 отделений государственного управления региона Померания в Польше. Анализ результатов позволил сделать выводы о значении вознаграждения в контексте других факторов удовлетворенности, а также оценить, как сравнительная оценка суммы вознаграждения может повлиять на взаимосвязь между вознаграждением и общей удовлетворенности работой

Regression of target organ damage in children and adolescents with primary hypertension

We assessed the effects of 12 months of non-pharmacological and pharmacological therapy on 24-h ambulatory blood pressure, regression of target organ damage (TOD) and metabolic abnormalities in 86 children (14.1 ± 2.4 years) with primary hypertension. Twenty-four hour systolic and diastolic blood pressure (BP) decreased (130 ± 8 vs 126 ± 8, 73 ± 7 vs 70 ± 7, p = 0.0001 and 0.004 respectively). Body mass index (BMI) did not change, but waist-to-hip (0.85 ± 0.07 vs 0.83 ± 0.05, p = 0.01) and waist-to-height ratio (WHtR; 0.49 ± 0.07 vs 0.48 ± 0.05, p = 0.008) decreased. Left ventricular mass index (LVMi; 38.5 ± 10.7 vs 35.2 ± 7.5 g/m2.7, p = 0.0001), prevalence of left ventricular hypertrophy (46.5% vs 31.4%; p = 0.0001), carotid intima-media thickness (cIMT; 0.44 ± 0.05 vs 0.42 ± 0.04 mm, p = 0.0001), wall cross sectional area (WCSA; 7.5 ± 1.3 vs 6.9 ± 1.2 mm2, p = 0.002), hsCRP (1.1 ± 1.0 vs 0.7 ± 0.7 mg/l, p = 0.002), and LDL-cholesterol (115 ± 33 vs 107 ± 26 mg/dl, p = 0.001) decreased. Patients who had lowered BP had a lower cIMT at the second examination (0.41 ± 0.04 vs 0.43 ± 0.04 mm, p = 0.04) and lower initial hsCRP values (0.9 ± 0.7 vs 1.5 ± 1.3 mg/l, p = 0.04) in comparison to non-responders. Regression analysis revealed that the main predictor of LVMi decrease was a decrease in abdominal fat expressed as a decrease in waist circumference (WC) (R2 = 0.280, β = 0.558, p = 0.005), for WCSA-SDS a decrease in WC (R2 = 0.332, β = 0.611, p = 0.009) and for a cIMT-SDS decrease the main predictor was a decrease in hsCRP concentrations (R2 = 0.137, β = 0.412, p = 0.03). Standard antihypertensive treatment lowered BP and led to regression of TOD in hypertensive children. Lean body mass increase and decrease in abdominal obesity correlated with TOD regression

Prevalence of left ventricular hypertrophy in children and young people with primary hypertension: Meta-analysis and meta-regression

Background: Left ventricular hypertrophy (LVH) is the main marker of HMOD in children and young people (CYP). We aimed to assess the prevalence of LVH and its determinants in CYP with primary hypertension (PH). Methods: A meta-analysis of prevalence was performed. A literature search of articles reporting LVH in CYP with PH was conducted in Medline, Embase, and Cochrane databases. Studies with a primary focus on CYP (up to 21 years) with PH were included. Meta-regression was used to analyze factors explaining observed heterogeneity. Results: The search yielded a total of 2,200 articles, 153 of those underwent full-text review, and 47 reports were included. The reports evaluated 51 study cohorts including 5,622 individuals, 73% male subjects, and a mean age of 13.6 years. LVH was defined as left ventricle mass index (LVMI) >= 95th percentile in 22 (47%), fixed cut-off >= 38.6 g/m(2.7) in eight (17%), sex-specific fixed cut-off values in six (13%), and miscellaneously in others. The overall prevalence of LVH was 30.5% (95% CI 27.2-33.9), while heterogeneity was high (I-2 = 84%). Subgroup analysis including 1,393 individuals (76% male subjects, mean age 14.7 years) from pediatric hypertension specialty clinics and LVH defined as LVMI >= 95th percentile only (19 study cohorts from 18 studies), reported prevalence of LVH at 29.9% (95% CI 23.9 to 36.3), and high heterogeneity (I-2 = 84%). Two studies involving patients identified through community screening (n = 1,234) reported lower LVH prevalence (21.5%). In the meta-regression, only body mass index (BMI) z-score was significantly associated with LVH prevalence (estimate 0.23, 95% CI 0.08-0.39, p = 0.004) and accounted for 41% of observed heterogeneity, but not age, male percentage, BMI, or waist circumference z-score. The predominant LVH phenotype was eccentric LVH in patients from specialty clinics (prevalence of 22% in seven studies with 779 participants) and one community screening study reported the predominance of concentric LVH (12%). Conclusion: Left ventricular hypertrophy is evident in at least one-fifth of children and young adults with PH and in nearly a third of those referred to specialty clinics with a predominant eccentric LVH pattern in the latter. Increased BMI is the most significant risk association for LVH in hypertensive youth

Knowledge gaps and future directions in cognitive functions in children and adolescents with primary arterial hypertension: A systematic review

Arterial hypertension (AH) among adults is known to be associated with worse cognitive outcomes. Similarly, children and adolescents with AH could be expected to underperform during neuropsychological evaluations when compared with healthy peers. Our aims were to review the existing literature on cognitive functioning among children and adolescents with primary AH and to identify what additional evidence may be needed to substantiate the impact of hypertension on poor cognitive outcomes in this population. We conducted a systematic review of articles in PubMed and Web of Science published before 17 January 2022, reporting on cognitive testing among children and adolescents with primary AH. From 1,316 records, 13 were included in the review-7 used battery-testing while other employed indirect measures of cognitive functions. Most of the studies reported worse results among individuals with AH. Results of two prospective trials suggested that cognitive functioning may improve after starting antihypertensive treatment. Ambulatory blood pressure monitoring was shown to be more strongly related to cognitive testing results than office measures of blood pressure. Significant confounders, namely obesity and sleep apnea, were identified throughout the studies. Our review indicates that evidence relating AH with poor cognitive functioning among youth is usually based on indirect measures of executive functions (e.g., questionnaires) rather than objective neuropsychological tests. Future prospective trials set to test different cognitive domains in children and adolescents undergoing treatment for AH are endorsed and should consider using standardized neuropsychological batteries as well as adjust the assessing results for obesity and sleep disorders