Recent palaeoenvironmental evidence for the processing of hemp (Cannabis sativa L.) in eastern England during the medieval period

[FIRST PARAGRAPH] Hemp (Cannabissativa L.)— whose origins as a domesticated plant probably lie in C.Asia — has been cultivated in England since at least a.d.800 (and before this perhaps in the Roman Period), mainly for its ¿bre, which was used to make sails, ropes, ¿shing nets and clothes, as well as for the oil from hempseed. Hemp cultivation may have reached a peak during the early 16th century, when Henry VIII decreed that increased hemp production was required to supply the expanding navy. Evidence for the locations where the crop was cultivated and processed is available in several different forms, including written evidence in parish records and government reports, place-name evidence (e.g.Hempholme and some instances of Hempstead), and features on old maps, such as Hempis¿eld (hemp¿eld)

Near infrared reflectance spectroscopy for the determination of free gossypol in cottonseed meal

Gossypol is a toxic polyphenolic compound produced by the pigment glands of the cotton plant. The free gossypol content of cottonseed meal (CSM) is commonly determined by the American Oil Chemists’ Society (AOCS) wet chemistry method. The AOCS method, however, laboratory-intensive, time-consuming, and therefore, not practical for quick field analyses. To determine if the free gossypol content of CSM could be predicted by near infrared reflectance spectroscopy (NIRS), CSM samples were collected from all over the world. All CSM samples were ground and a portion of each analyzed for free gossypol by the AOCS procedure (reference data) and by NIRS (reflectance data). Both reflectance and reference data were combined in calibration. The coefficient of determination (r2) and standard error of prediction (SEP) were used to assess the calibration accuracy. The r2 was 0.728, and the SEP was 0.034 for the initial calibration that included samples from all over the world. However, the r2 and SEP improved to 0.921 and 0.014, respectively, if the calibration was made using CSM samples only from the United States. These results indicate that a general prediction equation can be developed to predict the free gossypol content of CSM by NIRS. From a practical standpoint, NIRS technology provides a method for quickly assessing whether a particular batch of CSM has a free gossypol content low enough to be suitable for use in poultry diets.This research was supported in part by grant 05-635GA from the Georgian Cotton Commission, Perry, G

Changes in the physico-chemical and microbial nature of wetlands from the leaching of chromated copper arsenate (CCA)-treated wood

Microbial activities are responsible for reducing the harmful effects of pollutants in different burial environments. Within wetlands in particular, microorganisms play an important role in the transformation of heavy metals and metalloids via direct or indirect oxidation/reduction. In turn, these microbial transformations can lead to the detoxification of pollutant elements such as copper, chromium and arsenic that comprise CCA-treated wood. CCA was the most commonly used wood preservative in the UK (up until its partial ban in 2004). CCA prolongs the service life of wood by making it resistant to microbiological attack. As such, it has been regularly used in the construction of platforms and boardwalks in wetlands. However, recent concerns over the impact of the chemical constituents of this treatment on both the environment and human health have prompted the introduction of legislation in order to ensure that this type of treated wood is disposed of in accordance with the relevant health and safety guidelines. In light of this information, it is important to assess changes in the physico-chemical and microbial nature of wetlands associated with the leaching of CCA from wooden structures. The results will not only provide a greater scope for understanding the implications associated with the in situ preservation of the archaeological resource contained within these environments, but also highlight the potential ramifications for wetland ecosystem dynamics

The emergence of the LBK: Migration, memory and meaning at the transition to agriculture

This paper represents one element of a collaborative research project, funded by the AHRC, which focussed on a multidisciplinary study of the Earlier Neolithic cemetery of Vedrovice, Moravia, Czech Republic. One of the key aims of this project was the generation of new knowledge in relation to the emergence of the Linear Pottery, or Linearbandkeramik (LBK) culture in Europe. The current paper focuses on the evidence for the shift from hunting and gathering to farming from the perspective of individual life histories and the transmission of knowledge through migration and cultural exchanges

Transcriptional Regulation in Yeast during Diauxic Shift and Stationary Phase

The preferred source of carbon and energy for yeast cells is glucose. When yeast cells are grown in liquid cultures, they metabolize glucose predominantly by glycolysis, releasing ethanol in the medium. When glucose becomes limiting, the cells enter diauxic shift characterized by decreased growth rate and by switching metabolism from glycolysis to aerobic utilization of ethanol. When ethanol is depleted from the medium, cells enter quiescent or stationary phase G0. Cells in diauxic shift and stationary phase are stressed by the lack of nutrients and by accumulation of toxic metabolites, primarily from the oxidative metabolism, and are differentiated in ways that allow them to maintain viability for extended periods of time. The transition of yeast cells from exponential phase to quiescence is regulated by protein kinase A, TOR, Snf1p, and Rim15p pathways that signal changes in availability of nutrients, converge on transcriptional factors Msn2p, Msn4p, and Gis1p, and elicit extensive reprogramming of the transcription machinery. However, the events in transcriptional regulation during diauxic shift and quiescence are incompletely understood. Because cells from multicellular eukaryotic organisms spend most of their life in G0 phase, understanding transcriptional regulation in quiescence will inform other fields, such as cancer, development, and aging