Validation of the questionnaire of the transtheoretical model of change of physical exercise

El objetivo de este estudio fue traducir y validar al español el Cuestionario del Modelo Transteórico del Cambio de Ejercicio Físico de Prochaska y DiClemente (1983). Se utilizó una muestra de 812 personas, entre los 14 y los 88 años (29,5±21,7). Se realizó un análisis factorial confirmatorio, análisis de consistencia interna y validez predictiva. Los resultados del Cuestionario del Modelo Transteórico del Cambio de Ejercicio Físico presentaron valores adecuados (χ2/g.l = 4,3, CFI = 0,92, IFI = 0,92, TLI = 0,90, RMSEA = 0,06, SRMR = 0,05). La consistencia interna mostró valores encima de .70. Se halló una predicción positiva y significativa de los estadios más activos del Modelo Transteórico sobre la intención de ser físicamente activo. Este estudio ha permitido proporcionar un cuestionario válido y fiable para evaluar el estadio en el que se encuentran las personas con respecto a la práctica de ejercicio físico, en el ámbito españolThe aim of this study was to translate and validate in Spanish the Questionnaire of the Transtheoretical Model of Change of Physical Exercise, of Prochaska and DiClemente (1983), and also to make adaptations and modifications as needed. The sample was composed of 812 people, aged between 14 and 88 years (29.5+21.7). Confirmative factorial analysis, analysis of internal consistency and of predictive validity were carried out. After the confirmative factorial analysis, the Questionnaire of the Theoretical Model of Change of Physical Exercise showed acceptable results (x2/g.1=4,3, CFI=0,92, IFI= 0.92, TLI = 0.90, RMSEA = 0.06, SRMR = 0,05). Similarly, internal consistency obtained from the respective dimensions showed values above .70. A positive and significant prediction of the most active stages of the Transtheoretical Model (action and maintenance) was found on the ‘intention to be physically active’. This study has enabled the provision of a valid and reliable questionnair

Transcontextual model of motivation in the preaching of healthy lifestyles

El presente trabajo examinó la aplicación del Modelo Transcontextual de la Motivación en la predicción de estilos de vida saludables de atletas veteranos. Se utilizó una muestra de 682 atletas veteranos portugueses de ambos géneros, de edades comprendidas entre los 30 y los 76 años (M=43.64; DT=8.25), dónde a través de cuestionarios se ha medido: la satisfacción de las necesidades psicológicas básicas, la motivación, las variables del comportamiento planeado y los estilos de vida saludables. De las conclusiones alcanzadas en este trabajo, son de destacar la relevancia de fomentar la necesidad psicológica básica de relación social, ya que ésta favorecerá la motivación intrínseca, promoviendo un mayor control del comportamiento sobre las intenciones de los practicantes, generando así mejores hábitos alimenticios, hábitos de descanso y menor consumo de tabacoThe present paper has examined the application of the Transcontextual Model of motivation in the prediction of healthy lifestyles of veteran athletes. A sample of 682 Portuguese veteran athletes of both sexes, aged between 30 and 76 years (M = 43.64; SD = 8.25), were administered the following questionnaires: satisfaction of needs basic psychological, self-determination motivation, planned behavioral variables and healthy lifestyles. From the conclusions reached in this work, it is important to emphasize the importance of fostering the basic psychological need of relatedness, since this will favor the intrinsic motivation, promoting greater control of behavior over the intentions of practitioners, thus generating more healthy eating habits, rest habits and lower tobacco consumptio

Caveolin-1 is a risk factor for postsurgery metastasis in preclinical melanoma models

Melanomas are highly lethal skin tumours that are frequently treated by surgical resection. However, the efficacy of such procedures is often limited by tumour recurrence and metastasis. Caveolin-1 (CAV1) has been attributed roles as a tumour suppressor, although in late-stage tumours, its presence is associated with enhanced metastasis. The expression of this protein in human melanoma development and particularly how the presence of CAV1 affects metastasis after surgery has not been defined. CAV1 expression in human melanocytes and melanomas increases with disease progression and is highest in metastatic melanomas. The effect of increased CAV1 expression can then be evaluated using B16F10 murine melanoma cells injected into syngenic immunocompetent C57BL/6 mice or human A375 melanoma cells injected into immunodeficient B6Rag1−/− mice. Augmented CAV1 expression suppresses tumour formation upon a subcutaneous injection, but enhances lung metastasis of cells injected into the tail vein in both models. A procedure was initially developed using B16F10 melanoma cells in C57BL/6 mice to mimic better the situation in patients undergoing surgery. Subcutaneous tumours of a defined size were removed surgically and local tumour recurrence and lung metastasis were evaluated after another 14 days. In this postsurgery setting, CAV1 presence in B16F10 melanomas favoured metastasis to the lung, although tumour suppression at the initial site was still evident. Similar results were obtained when evaluating A375 cells in B6Rag1−/− mice. These results implicate CAV1 expression in melanomas as a marker of poor prognosis for patients undergoing surgery as CAV1 expression promotes experimental lung metastasis in two different preclinical models

Encapsulation of gold nanostructures and oil-in-water nanocarriers in microgels with biomedical potential

Indexación: Scopus.Funding: This research was funded by FONDECYT 1161450, 1150744, 11130494 and 1170929, FONDEQUIP EQM160157, EQM170111, CONICYT-FONDAP 15130011, and CONICYT PhD Scholarship 21141137.Here we report the incorporation of gold nanostructures (nanospheres or nanorods, functionalized with carboxylate-end PEG) and curcumin oil-in-water (O/W) nanoemulsions (CurNem) into alginate microgels using the dripping technique. While gold nanostructures are promising nanomaterials for photothermal therapy applications, CurNem possess important pharmacological activities as reported here. In this sense, we evaluated the effect of CurNem on cell viability of both cancerous and non-cancerous cell lines (AGS and HEK293T, respectively), demonstrating preferential toxicity in cancer cells and safety for the non-cancerous cells. After incorporating gold nanostructures and CurNem together into the microgels, microstructures with diameters of 220 and 540 µm were obtained. When stimulating microgels with a laser, the plasmon effect promoted a significant rise in the temperature of the medium; the temperature increase was higher for those containing gold nanorods (11–12 ◦ C) than nanospheres (1–2 ◦ C). Interestingly, the incorporation of both nanosystems in the microgels maintains the photothermal properties of the gold nanostructures unmodified and retains with high efficiency the curcumin nanocarriers. We conclude that these results will be of interest to design hydrogel formulations with therapeutic applications. © 2018 by the authors.https://www.mdpi.com/1420-3049/23/5/120

Development, cross-cultural adaptation, and psychometric characteristics of the persian progressive aphasia language scale in patients with primary progressive aphasia: A pilot study

Introduction: Primary Progressive Aphasia (PPA) is a neurological condition characterized by progressive dissolution of language capabilities. The Progressive Aphasia Language Scale (PALS) is an easy-to-apply bedside clinical scale capable of capturing and grading the key language features essential for the classification of PPA. The objective of the present study was to develop and validate the Persian version of the PALS (PALS-P) as a clinical language assessment test. Methods: In this cross-sectional study, PALS was translated and adapted into Persian according to the international guidelines. A total of 30 subjects (10 subjects with PPA and 20 control subjects without dementia) were recruited to evaluate the intra-rater reliability and discriminant validity of PALS-P. Results: The intra-rater reliability of the PALS-P within a 14-day interval was excellent for each subtest (ICC agreement range=0.81-1.0). PALS-P results were statistically significant among groups, suggesting its discriminative validity. Conclusion: This preliminary study indicates that PALS-P was successfully developed and translated. It seems to be a valid and reliable screening tool to assess language skills in Persian-speaking subjects with progressive aphasia

Quantum algorithm and circuit design solving the Poisson equation

The Poisson equation occurs in many areas of science and engineering. Here we focus on its numerical solution for an equation in d dimensions. In particular we present a quantum algorithm and a scalable quantum circuit design which approximates the solution of the Poisson equation on a grid with error \varepsilon. We assume we are given a supersposition of function evaluations of the right hand side of the Poisson equation. The algorithm produces a quantum state encoding the solution. The number of quantum operations and the number of qubits used by the circuit is almost linear in d and polylog in \varepsilon^{-1}. We present quantum circuit modules together with performance guarantees which can be also used for other problems.Comment: 30 pages, 9 figures. This is the revised version for publication in New Journal of Physic

Modelling \u3ci\u3eLeucaena\u3c/i\u3e Biomass Under Rainfed Production Systems of Semiarid Regions

The climatic variability of semiarid regions is the main source of uncertainties associated with forage and animal production, indicating a need for tools that accurately estimate forage production in order to construct a forage budgeting plan for livestock. This study simulated the biomass of Leucaena (Leucaena leucocephala) using the PHYGROW model in four locations located in Brazilian Semiarid. The work was carried out based on field data collected from 2019 to 2021. After sowing in 2018, leucaena was harvested when it reached 200 cm and to a target residual height of 70 cm. The biomass (fresh matter) was weighed, sampled and dried to obtain the total forage biomass (BFT) of each sample. The BFT was also estimated using the PHYGROW model, with field data being used to parameterize, calibrate and validate the model. The model performance, in turn, was evaluated based on the mean forecast error (BIAS %), root mean square error (RMSE) and Willmott index. Afterwards, a BFT time series was downloaded for each location, with the highest biomass simulated for each year being evaluated in the @Risk regarding their probability distribution. Thereafter, probability calculations of biomass production were performed, based on different levels of warranty in SigmaPlot software (11.0). The model underestimated the BFT collected in two locations and overestimated BFT in the others. The Weibull function was the best one to describe the data. Regarding biomass production under a 95% natural warranty, it was observed that leucaena showed low variation among locations (2240 ± 752 kg of DM ha-1 year-1 ). The PHYGROW model accurately predicted the leucaena BFT which, in turn, demonstrated significant adaptation potential to the various soil and climate conditions of Brazilian Semiarid. The use of probability analysis can contribute to forage planning, thus reducing the uncertainties related to climate variability, especially in rainfed production systems of dry areas

Increased acinar damage of salivary glands of patients with Sjögren's syndrome is paralleled by simultaneous imbalance of matrix metalloproteinase 3/tissue inhibitor of metalloproteinases 1 and matrix metalloproteinase 9/tissue inhibitor of metalloproteinases 1 ratios

Indexación: Scopus.Objective. Previous findings in labial salivary glands (LSGs) from patients with Sjögren's syndrome (SS) suggest that increased activity and expression of matrix metalloproieinase 9 (MMP-9) and MMP-3 trigger the destruction of acinar structures in these glands. Tissue inhibitors of matrix metalloproteinases (TIMPs) tightly control MMP activity, and TIMP expression is an important modulator of effects attributed to MMPs. This study was undertaken to investigate the correlation between the balance of MMPs/TIMPs in the LSGs of SS patients and the degree of inflammatory infiltration and acinar structure integrity. Methods. Three groups of SS patients classified according to focus score and residual tissue were studied. The expression of MMP-2, MMP-3, MMP-9, TIMP-1, and TIMP-2 was examined at the messenger RNA and protein levels. The ratio of MMP/TIMP expression (R value) was calculated. Focus score and acinar structure were evaluated by histologic analysis. Results. In SS patients the MMP-3/TIMP-1 ratio was higher than 1 and the MMP-9/TIMP-1 ratio was much higher than 1 whereas the MMP-2/TIMP-2 ratio nearly equaled 1, suggesting elevated proteolytic activity due mainly to MMP-9. R values were independent of the focus score of inflammatory cells, but correlated well with the dramatic changes observed in morphologic integrity of acini, as revealed mainly by the lack of nuclear polarity. Acinar changes were more evident when R values for both MMP-9/TIMP-1 and MMP-3/TIMP-1 were higher. Conclusion. This study provides evidence that an altered balance between MMPs and their inhibitors is associated with acinar damage. Since salivary gland acinar cells express both MMPs and TIMPs, these cells may play an important role in extracellular matrix destruction and in the LSG pathophysiology in SS.https://onlinelibrary.wiley.com/doi/full/10.1002/art.2126