The “Ayde of his Muses?”The Renaissance of John Florio and William Shakespeare

Jeremy Lester’s essay focuses on John Florio, arguing a far deeper implication of the prominent linguist and translator of Montaigne in the production of the Shakespearean oeuvre than previously thought. Although known by specialists, until not long ago, Florio was considered a secondary figure within the intellectual and artistic panorama of the Elizabethan and Jacobean times. After examining closely the life and works of Florio in accordance with Lamberto Tassinari’s book John Florio. The Man Who Was Shakespeare (Giano Books, 2009), Lester discusses the case of British scholar Saul Frampton of Westminster University who in two feature articles published in the London Guardian in July and August, 2013, asserted that John Florio was the editor-in-chief of Shakespeare’s collected plays (the First Folio, 1623). According to Frampton, this role allowed him to “censor,”“change” or “supplement” the original works of Shakespeare. Ben Jonson, the main instigator in the publication of the First Folio, was also a close friend and devotee of Florio, of whom he states in a dedication to a copy of his Volpone, that he was “an Ayde of his Muses.” Analyzing Tassinari’s theory, Lester comes to the conclusion that Florio, more than the editor and “Ayde” to the Bard, has a very good claim to be considered the author under the pseudonym Shakespeare. Tassinari’s book, now translated into French with the title John Florio alias Shakespeare (Le Bord de L’Eau, 2016), is sparking an animated debate within the French media

Functional expression of the yeast alpha-factor receptor in Xenopus oocytes

The STE2 gene of the yeast Saccharomyces cerevisiae encodes a 431- residue polypeptide that has been shown by chemical cross-linking and genetic studies to be a component of the receptor for the peptide mating pheromone, alpha-factor. To demonstrate directly that the ligand binding site of the alpha-factor receptor is comprised solely of the STE2 gene product, the STE2 protein was expressed in Xenopus oocytes. Oocytes microinjected with synthetic STE2 mRNA displayed specific surface binding for 35S-labeled alpha-factor (up to 40 sites/micron2/ng RNA). Oocytes injected with either STE2 antisense RNA or heterologous receptor mRNA (nicotinic acetylcholine receptor alpha, beta, gamma, and delta subunit mRNAs) showed no binding activity (indistinguishable from uninjected control oocytes). The apparent KD (7 nM) of the alpha-factor binding sites expressed on the oocyte surface, determined by competition binding studies, agreed with the values reported for intact yeast cells and yeast plasma membrane fractions. These findings demonstrate that the STE2 gene product is the only yeast polypeptide required for biogenesis of a functional alpha-factor receptor. Electrophysiological measurements indicated that the membrane conductance of oocytes injected with STE2 mRNA, or with both STE2 and GPA1 (encoding a yeast G protein alpha-subunit) mRNAs, did not change and was not affected by pheromone binding. Thus, the alpha-factor receptor, like mammalian G protein-coupled receptors, apparently lacks activity as an intrinsic or ligand-gated ion channel. This report is the first instance in which a membrane-bound receptor from a unicellular eukaryote has been expressed in a vertebrate cell

Gold substrate-induced single-mode lasing of GaN nanowires

We demonstrate a method for mode-selection by coupling a GaN nanowire laser to an underlying gold substrate. Multimode lasing of GaN nanowires is converted to single-mode behavior following placement onto a gold film. A mode-dependent loss is generated by the absorbing substrate to suppress multiple transverse-mode operation with a concomitant increase in lasing threshold of only ∼13%. This method provides greater flexibility in realizing practical single-mode nanowire lasers and offers insight into the design of metal-contacted nanoscale optoelectronics

Evaluation of an integrated plantation forestry and beef production system

The profitability of timber plantations and combined timber and grazing enterprises were compared to conventional grazing. Scenarios were considered at two sites (one near Kingaroy and one near Nambour) where hardwood plantation forests have been established. At each site a number of contrasting variables were considered. These were: (1) plantation rotation lengths (20, 25 and 30 years); (2) plantation establishment costs (high and low); and (3) prices paid for the timber at harvest (high and low stumpage price). A spreadsheet-based investment analysis tool was used to compare these scenarios. The marginal NPV (net present value) and marginal IRR (internal rate of return) were calculated to indicate the difference in return between the traditional land use of grazing and the introduction of plantation hardwood trees into the traditional just grazing system. Marginal internal rate of return, which is a measure of the marginal return to the extra funds invested, ranged from 0.46% under the most pessimistic option without cattle, to 10.7% under the most optimistic option with cattle, at the Kingaroy site, and from 2.8% to 13.3% under contrasting options at the Nambour site. The more profitable scenarios occurred when planted forests were coupled with cattle grazing systems. The internal rate of return and net present value were particularly sensitive to the price received for the final product when the trees are harvested. Engineered wood products may provide a future high-value option for the hardwood plantation resource and revenues from commercial thinning may be brought forward if an existing technology (i.e. spindle-less lathe technology) is taken up by industry. Expansion of hardwood plantation forests could help meet the increasing demand for hardwood products in Australia if investments in plantation forests are made. The scenarios considered here demonstrate that there is potential for profitable agroforestry systems in the two regions where plantation forests have been successfully established

A high-resolution infrared spectroscopic investigation of the halogen atom-HCN entrance channel complexes solvated in superfluid helium droplets

Rotationally resolved infrared spectra are reported for the X-HCN (X = Cl, Br, I) binary complexes solvated in helium nanodroplets. These results are directly compared with that obtained previously for the corresponding X-HF complexes [J. M. Merritt, J. K\"upper, and R. E. Miller, PCCP, 7, 67 (2005)]. For bromine and iodine atoms complexed with HCN, two linear structures are observed and assigned to the $^{2}\Sigma_{1/2}$ and $^{2}\Pi_{3/2}$ ground electronic states of the nitrogen and hydrogen bound geometries, respectively. Experiments for HCN + chlorine atoms give rise to only a single band which is attributed to the nitrogen bound isomer. That the hydrogen bound isomer is not stabilized is rationalized in terms of a lowering of the isomerization barrier by spin-orbit coupling. Theoretical calculations with and without spin-orbit coupling have also been performed and are compared with our experimental results. The possibility of stabilizing high-energy structures containing multiple radicals is discussed, motivated by preliminary spectroscopic evidence for the di-radical Br-HCCCN-Br complex. Spectra for the corresponding molecular halogen HCN-X$_{2}$ complexes are also presented.Comment: 20 pages, 15 figures, 6 tables, RevTe

Entrance Channel X-HF (X=Cl, Br, and I) Complexes studied by High-Resolution Infrared Laser Spectroscopy in Helium Nanodroplets

Rotationally resolved infrared spectra are reported for halogen atom - HF free radical complexes formed in helium nanodroplets. An effusive pyrolysis source is used to dope helium droplets with Cl, Br and I atoms, formed by thermal dissociation of Cl$_2$, Br$_2$ and I$_2$. A single hydrogen fluoride molecule is then added to the droplets, resulting in the formation of the X-HF complexes of interest. Analysis of the resulting spectra confirms that the observed species have $^2\Pi_{3/2}$ ground electronic states, consistent with the linear hydrogen bound structures predicted from theory. Stark spectra are also reported for these species, from which the permanent electric dipole moments are determined.Comment: 41 pages, 16 figures, 5 table

Gothic visions of classical architecture in Hablot Knight Browne’s “dark” illustrations for the novels of Charles Dickens

In the early gothic literature of the eighteenth century danger lurked in the darkness beneath the pointed arches of gothic buildings. During the nineteenth century there was a progressive, although never complete, dislocation of gothic literary readings from gothic architecture. This article explores a phase in that development through discussion of a series of ‘dark’ illustrations produced by Hablot Knight Browne to illustrate novels by Charles Dickens. These show the way in which the rounded arches of neo-classical architecture were depicted in the mid-nineteenth century as locales of oppression and obscurity. Such depictions acted, in an age of political and moral reform, to critique the values of the system of power and authority that such architecture represented