Polyploidy breaks speciation barriers in Australian burrowing frogs Neobatrachus

Polyploidy has played an important role in evolution across the tree of life but it is still unclear how polyploid lineages may persist after their initial formation. While both common and well-studied in plants, polyploidy is rare in animals and generally less understood. The Australian burrowing frog genus Neobatrachus is comprised of six diploid and three polyploid species and offers a powerful animal polyploid model system. We generated exome-capture sequence data from 87 individuals representing all nine species of Neobatrachus to investigate species-level relationships, the origin and inheritance mode of polyploid species, and the population genomic effects of polyploidy on genus-wide demography. We describe rapid speciation of diploid Neobatrachus species and show that the three independently originated polyploid species have tetrasomic or mixed inheritance. We document higher genetic diversity in tetraploids, resulting from widespread gene flow between the tetraploids, asymmetric inter-ploidy gene flow directed from sympatric diploids to tetraploids, and isolation of diploid species from each other. We also constructed models of ecologically suitable areas for each species to investigate the impact of climate on differing ploidy levels. These models suggest substantial change in suitable areas compared to past climate, which correspond to population genomic estimates of demographic histories. We propose that Neobatrachus diploids may be suffering the early genomic impacts of climate-induced habitat loss, while tetraploids appear to be avoiding this fate, possibly due to widespread gene flow. Finally, we demonstrate that Neobatrachus is an attractive model to study the effects of ploidy on the evolution of adaptation in animals

Codeless GPS Applications to Multi-Path: CGAMP

Cordless Global Positioning System (GPS) Applications to Multi-Path (CGAMP) is meeting the challenge of exploiting the L-band signals from the Global Positioning System (GPS) satellites for the measurement of the impulse response of radio transmission channels over space-Earth paths. This approach was originally suggested by E. K. Smith and has been pursued by J. Lemmon, without an affordable implementation being identifiable. In addition to the high cost of a suitable P code correlating GPS receiver, there is also the major impediment of the often announced Department of Defense policy of selective availability/anti-spoof (SA/AS) that clouds reliable access to the wideband (20 MHz) P channel of the GPS signals without cryptographic access. A technique proposed by MacDoran utilizes codeless methods for exploiting the P channel signals implemented by the use of a pair of antennas and cross correlation signal detection

Molecular Density Functional Theory of Water describing Hydrophobicity at Short and Long Length Scales

We present an extension of our recently introduced molecular density functional theory of water [G. Jeanmairet et al., J. Phys. Chem. Lett. 4, 619, 2013] to the solvation of hydrophobic solutes of various sizes, going from angstroms to nanometers. The theory is based on the quadratic expansion of the excess free energy in terms of two classical density fields, the particle density and the multipolar polarization density. Its implementation requires as input a molecular model of water and three measurable bulk properties, namely the structure factor and the k-dependent longitudinal and transverse dielectric susceptibilities. The fine three-dimensional water structure around small hydrophobic molecules is found to be well reproduced. In contrast the computed solvation free-energies appear overestimated and do not exhibit the correct qualitative behavior when the hydrophobic solute is grown in size. These shortcomings are corrected, in the spirit of the Lum-Chandler-Weeks theory, by complementing the functional with a truncated hard-sphere functional acting beyond quadratic order in density. It makes the resulting functional compatible with the Van-der-Waals theory of liquid-vapor coexistence at long range. Compared to available molecular simulations, the approach yields reasonable solvation structure and free energy of hard or soft spheres of increasing size, with a correct qualitative transition from a volume-driven to a surface-driven regime at the nanometer scale.Comment: 24 pages, 8 figure

Probing the hydrogen melting line at high pressures by dynamic compression

We investigate the capabilities of dynamic compression by intense heavy ion beams to yield information about the high pressure phases of hydrogen. Employing ab initio simulations and experimental data, a new wide range equation of state for hydrogen is constructed. The results show that the melting line up to its maximum as well as the transition from molecular fluids to fully ionized plasmas can be tested with the beam parameters soon to be available. We demonstrate that x-ray scattering can distinguish between phases and dissociation states

Effects of Growing-Season Prescribed Burning on Vigor of Sericea Lespedeza in the Kansas Flint Hills: I. Suppression of Seed Production and Canopy Dominance

Sericea lespedeza (Lespedeza cuneata) is a highly fecund noxious weed in Kansas and surrounding states. Individual plants are capable of producing greater than 1,000 seeds annually. Vigorous seed production allows sericea lespedeza to rapidly infiltrate native and cultivated grasslands; seed can be transported great distances via farm machinery and the alimentary canal of wild and domestic herbivores. In Kansas alone, sericea lespedeza infests more than 700 square miles of pasture, primarily in the Flint Hills region. The resulting damage to native habitats for wildlife and pasture quality for domestic herbivores has been devastating.The predominant grazing management practice in the Kansas Flint Hills involves annual spring burning in April followed by intensive grazing with yearling beef cattle for a relatively short period from late April to August. During seasonal grazing, 40 to 60% of annual graminoid production is removed and grazing lands then remain idle for the remainder of the year. Under this prevailing management practice, invasion by sericea lespedeza into the tallgrass prairie biome has steadily increased. Oklahoma State University researchers speculated that dormant-season, spring fires may stimulate sericea lespedeza seed germination by scarifying seeds cast the previous fall. Previous research reported that application of growing season fire at 3 year intervals decreased the rate of sericea lespedeza invasion. Therefore, the objective of our study was to evaluate the effects of annual prescribed burning applied during the growing season on vigor of sericea lespedeza infesting native tallgrass range

Effects of Growing-Season Prescribed Burning on Vigor of Sericea Lespedeza in the Kansas Flint Hills: II. Plant-Species Composition

Fire has, for centuries, been a key force for sustainability of native ecosystems in the Kansas Flint Hills. Prior to the arrival of European settlers, prescribed and wild fires occurred at less than 3-year intervals in the tallgrass prairie region. As a result, native tallgrass plant communities adapted to fire at regular intervals and plant-species composition became stable on a geologic time scale.Currently, prescribed fire is used in the Kansas Flint Hills as a treatment for control of woody-stemmed invasive species such as eastern red cedar, honey locust, and roughleaf dogwood. These fires are generally applied in March and April and have become an integral part of the most common grazing management practice in the Kansas Flint Hills: annual spring burning in April followed by intensive grazing with yearling beef cattle for a relatively short period of time from late April to early August. Annual burning reportedly results in 0.2 to 0.3 lb of additional daily weight gain for yearling cattle when used in that way. In contrast, prescribed and wild fires during the presettlement era were not concentrated during any particular season of the year.Use of prescribed burning that is limited to a short interval during the spring has coincided with a steady increase of an invasive, non-woody, perennial legume known as sericea lespedeza (Lespedeza cuneata). Introduced into North America during the late 19th century, sericea lespedeza has proven highly adaptable to Flint Hills soils and climate. Prolific seed production appears to be the primary means of invasion. Seeds of sericea lespedeza are not wind-borne but are easily transported via the digestive tract of tannin-resistant herbivores and via machinery.Until recently, control of sericea lespedeza has relied heavily on costly, repeated application of herbicides, which has not checked the spread of the plant. We previously reported that prescribed burning during the months of August and September had strong suppressive effects on stand vigor and seed production of sericea lespedeza at a greatly reduced cost compared with herbicide. Questions remain, however, about the effects of growing-season prescribed burning on non-target plant species and soil cover. Therefore, the objective of this study was to evaluate the effects of growing-season prescribed fire on soil cover and populations of native grasses, forbs, and shrubs

Effects of Intensive Late-Season Sheep Grazing Following Early-Season Steer Grazing on Population Dynamics of Sericea Lespedeza in the Kansas Flint Hills

Sericea lespedeza (Lespedeza cuneata; SL) is a high-tannin, invasive forb in the Tallgrass Prairie ecosystem. In Kansas, sericea lespedeza infests 980 square miles of pasture, primarily in the Flint Hills region. Sericea lespedeza infestations reduce native grass production by up to 92% through a combination of aggressive growth, prolific reproduction, canopy dominance, and chemical inhibition (allelopathy). Herbicides retard the spread of sericea lespedeza, but application is laborious and expensive; moreover, herbicides are lethal to ecologically-important, non-target plant species. Increased grazing pressure on sericea lespedeza by domestic herbivores may slow its spread and facilitate some measure of biological control. Unfortunately, mature plants contain high levels of condensed tannins, which are a strong deterrent to grazing by beef cattle. Small ruminants have greater tolerance for condensed tannins than beef cattle. Sheep, in particular, appear less susceptible to certain plant toxins than beef cattle and may be useful to selectively pressure noxious weeds like sericea lespedeza. The predominant grazing management practice in the Flint Hills region of Kansas involves annual spring burning followed by intensive grazing with yearling beef cattle from April to August. During seasonal grazing, 40 to 60% of annual graminoid production is removed and pastures remain idle for the remainder of the year. Under this prevailing management practice, invasion by sericea lespedeza into the Tallgrass Prairie biome has steadily increased. Sericea lespedeza flowers and produces seed in late summer from August to September. The absence of grazing pressure during this interval strongly promotes seed production, seed distribution, and continued invasion of the Flint Hills ecoregion by this noxious weed. Therefore, the objective of our study was to evaluate effects of late-season sheep grazing following locally-conventional steer grazing on vigor and reproductive capabilities of sericea lespedeza

Effects of Growing-Season Prescribed Burning on Vigor of the Noxious Weed Sericea Lespedeza (Lespedeza cuneata) in the Kansas Flint Hills

Sericea lespedeza (SL) was introduced into the United States from Asia in the late 19th century. Early land managers recognized that SL was adaptable; tolerant of shallow, acidic or low-fertility soils; and resistant to insects and disease. This combination of traits made SL a widely-used plant for reseeding strip-mined lands, highway right-ofways, dams, and waterways in the US for nearly a century. Regrettably, SL is highly fecund. Individual plants are capable of producing up to 850 lb of seed per acre annually. Vigorous seed production allows SL to rapidly infiltrate native grasslands that are adjacent to reseeding projects; seed can be transported great distances via the alimentary canal and hair of wild and domestic herbivores. In Kansas alone, SL has infested approximately 980 square miles of pasture, primarily in the Flint Hills region. The resulting damage to native habitats for wildlife and pasture quality for domestic herbivores has been devastating. The predominant grazing management practice in the Kansas Flint Hills involves annual spring burning in March or April, followed by intensive grazing with yearling beef cattle for a relatively short period from April to August. During seasonal grazing, 40 to 60% of annual graminoid production is removed and grazing lands then remain idle for the remainder of the year. Under this prevailing management practice, invasion by SL into the Tallgrass Prairie biome has steadily increased. Oklahoma State University researchers speculated that dormant-season, spring fires may stimulate SL growth by scarifying seeds lying on the surface of the soil. In contrast, plants with robust canopies respond more strongly to growing-season prescribed burns than to dormant-season prescribed burns. Previous research reported that application of growing season fire at 3-yr intervals decreased the rate of SL invasion. Therefore, the objective of our study was to evaluate the effects of growing-season prescribed burning of native tallgrass range on vigor of sericea lespedeza

Statistical Mechanics of Membrane Protein Conformation: A Homopolymer Model

The conformation and the phase diagram of a membrane protein are investigated via grand canonical ensemble approach using a homopolymer model. We discuss the nature and pathway of $\alpha$-helix integration into the membrane that results depending upon membrane permeability and polymer adsorptivity. For a membrane with the permeability larger than a critical value, the integration becomes the second order transition that occurs at the same temperature as that of the adsorption transition. For a nonadsorbing membrane, the integration is of the first order due to the aggregation of $\alpha$-helices.Comment: RevTeX with 5 postscript figure

Performance of a cryogenic system prototype for the XENON1T Detector

We have developed an efficient cryogenic system with heat exchange and associated gas purification system, as a prototype for the XENON1T experiment. The XENON1T detector will use about 3 ton of liquid xenon (LXe) at a temperature of 175K as target and detection medium for a dark matter search. In this paper we report results on the cryogenic system performance focusing on the dynamics of the gas circulation-purification through a heated getter, at flow rates above 50 Standard Liter per Minute (SLPM). A maximum flow of 114 SLPM has been achieved, and using two heat exchangers in parallel, a heat exchange efficiency better than 96% has been measured