Morphology of seismically slow lower-mantle structures

Large low shear velocity provinces (LLSVPs), whose origin and dynamic implication remain enigmatic, dominate the lowermost mantle. For decades, seismologists have created increasingly detailed pictures of the LLSVPs through tomographic models constructed with different modeling methodologies, data sets, parametrizations and regularizations. Here, we extend the cluster analysis methodology of Lekic $\textit{et al.}$, to classify seismic mantle structure in five recent global shear wave speed ($\textit{V}_S$) tomographic models into three groups. By restricting the analysis to moving depth windows of the radial profiles of $\textit{V}_S$, we assess the vertical extent of features. We also show that three clusters are better than two (or four) when representing the entire lower mantle, as the boundaries of the three clusters more closely follow regions of high lateral $\textit{V}_S$ gradients. Qualitatively, we relate the anomalously slow cluster to the LLSVPs, the anomalously fast cluster to slab material entering the lower mantle and the neutral cluster to ‘background’ lower mantle material. We obtain compatible results by repeating the analysis on recent global $\textit{P}$-wave speed ($\textit{V}_P$) models, although we find less agreement across $\textit{V}_P$ models. We systematically show that the clustering results, even in detail, agree remarkably well with a wide range of local waveform studies. This suggests that the two LLSVPs consist of multiple internal anomalies with a wide variety of morphologies, including shallowly to steeply sloping, and even overhanging, boundaries. Additionally, there are indications of previously unrecognized meso-scale features, which, like the Perm anomaly, are separated from the two main LLSVPs beneath the Pacific and Africa. The observed wide variety of structure size and morphology offers a challenge to recreate in geodynamic models; potentially, the variety can result from various degrees of mixing of several compositionally distinct components. Finally, we obtain new, much larger estimates of the volume/mass occupied by LLSVPs— 8.0 per cent ±0.9 ($\mu$ ± 1$\sigma$) of whole mantle volume and 9.1 per cent ±1.0 ($\mu$ ± 1$\sigma$) of whole mantle mass—and discuss implications for associating the LLSVPs with the hidden reservoir enriched in heat producing elements.National Science Foundation (EAR1352214), Packard Foundation, Pembroke College, Cambridge (Drapers’ Company Research Fellowship

Geo-neutrinos and Earth Models

We present the current status of geo-neutrino measurements and their implications for radiogenic heating in the mantle. Earth models predict different levels of radiogenic heating and, therefore, different geo-neutrino fluxes from the mantle. Seismic tomography reveals features in the deep mantle possibly correlated with radiogenic heating and causing spatial variations in the mantle geo-neutrino flux at the Earth surface. An ocean-based observatory offers the greatest sensitivity to the mantle flux and potential for resolving Earth models and mantle features. Refinements to estimates of the geo-neutrino flux from continental crust reduce uncertainty in measurements of the mantle flux, especially measurements from land-based observatories. These refinements enable the resolution of Earth models using the combined measurements from multiple continental observatories.Comment: 9 pages, 4 figures; Contributed paper TAUP 201

Compositional layering within the large low shear-wave velocity provinces in the lower mantle

The large low shear‐wave velocity provinces (LLSVP) are thermochemical anomalies in the deep Earth's mantle, thousands of km wide and ∼1800 km high. This study explores the hypothesis that the LLSVPs are compositionally subdivided into two domains: a primordial bottom domain near the core‐mantle boundary and a basaltic shallow domain that extends from 1100 to 2300 km depth. This hypothesis reconciles published observations in that it predicts that the two domains have different physical properties (bulk‐sound versus shear‐wave speed versus density anomalies), the transition in seismic velocities separating them is abrupt, and both domains remain seismically distinct from the ambient mantle. We here report underside reflections from the top of the LLSVP shallow domain, supporting a compositional origin. By exploring a suite of two‐dimensional geodynamic models, we constrain the conditions under which well‐separated “double‐layered” piles with realistic geometry can persist for billions of years. Results show that long‐term separation requires density differences of ∼100 kg/m³ between LLSVP materials, providing a constraint for origin and composition. The models further predict short‐lived “secondary” plumelets to rise from LLSVP roofs and to entrain basaltic material that has evolved in the lower mantle. Long‐lived, vigorous “primary” plumes instead rise from LLSVP margins and entrain a mix of materials, including small fractions of primordial material. These predictions are consistent with the locations of hot spots relative to LLSVPs, and address the geochemical and geochronological record of (oceanic) hot spot volcanism. The study of large‐scale heterogeneity within LLSVPs has important implications for our understanding of the evolution and composition of the mantle

Lipid Composition and Antioxidant Activities of the Seed Oil from Three Malvaceae Species

The oil content and fatty acids, unsaponifiable composition and antioxidant activities of the seed oil from three Malvaceae species (Malva sylvestris L., Malva sylvestris L. var. mauritiana and Althaea officinalis L.) from Serbia were determined. The oil yields from the seeds varied from 7.18 to 9.60%. The main fatty acids of the seed oils were linoleic acid (44.14-54.49%), oleic acid (13.00-16.99%) and palmitic acid (11.45-24.29%). A small amount of cyclopropenoid acids, up to 1.85% was also established. The predominant sterol in all seed oils was beta-sitosterol (11.51-17.34 mg/g of oil). The antioxidant potential of all the investigated seed oils was evaluated by radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay

The Far Side of Mars: Two Distant Marsquakes Detected by InSight

For over three Earth years the Marsquake Service has been analyzing the data sent back from the Seismic Experiment for Interior Structure¿the seismometer placed on the surface of Mars by NASA¿s InSight lander. Although by October 2021, the Mars seismic catalog included 951 events, until recently all these events have been assessed as lying within a radius of 100° of InSight. Here we report two distant events that occurred within days of each other, located on the far side of Mars, giving us our first glimpse into Mars¿ core shadow zone. The first event, recorded on 25 August 2021 (InSight sol 976), shows clear polarized arrivals that we interpret to be PP and SS phases at low frequencies and locates to Valles Marineris, 146° ± 7° from InSight. The second event, occurring on 18 September 2021 (sol 1000), has significantly more broadband energy with emergent PP and SS arrivals, and a weak phase arriving before PP that we interpret as Pdiff¿. Considering uncertain pick times and poorly constrained travel times for Pdiff¿, we estimate this event is at a distance between 107° and 147° from InSight. With magnitudes of MMaw 4.2 and 4.1, respectively, these are the largest seismic events recorded so far on Mars.Anna C. Horleston, Jessica C. E. Irving,and Nicholas A. Teanby are funded by the UKSA under Grant Numbers ST/R002096/1, ST/W002523/1, and ST/W002515/1.Nikolaj L. Dahmen, Cecilia Duran, Géraldine Zenhäusern, andSimon C. Stähler would like to acknowledge support from Eidgenössische Technische Hochschule (ETH) through the ETH+ funding scheme (ETH+02 19-1: “Planet Mars”). The French coauthors acknowledge the funding support provided by CNES and the Agence Nationale de la Recherche (ANR-19-CE31-0008-08 MAGIS) for SEIS operation and SEIS Science analysis. Alexander E. Stott acknowledges the French Space Agency CNES and ANR (ANR-19-CE31-0008-08). Caroline Beghein and Jiaqi Li were supported by NASA InSight Participating Scientist Program (PSP) Grant Number 80NSSC18K1679. This article is InSight Contribution Number 236

Expression of Msx-1 is suppressed in bisphosphonate associated osteonecrosis related jaw tissue-etiopathology considerations respecting jaw developmental biology-related unique features

<p>Abstract</p> <p>Background</p> <p>Bone-destructive disease treatments include bisphosphonates and antibodies against the osteoclast differentiator, RANKL (aRANKL); however, osteonecrosis of the jaw (ONJ) is a frequent side-effect. Current models fail to explain the restriction of bisphosphonate (BP)-related and denosumab (anti-RANKL antibody)-related ONJ to jaws. Msx-1 is exclusively expressed in craniofacial structures and pivotal to cranial neural crest (CNC)-derived periodontal tissue remodeling. We hypothesised that Msx-1 expression might be impaired in bisphosphonate-related ONJ. The study aim was to elucidate Msx-1 and RANKL-associated signal transduction (BMP-2/4, RANKL) in ONJ-altered and healthy periodontal tissue.</p> <p>Methods</p> <p>Twenty ONJ and twenty non-BP exposed periodontal samples were processed for RT-PCR and immunohistochemistry. An automated staining-based alkaline phosphatase-anti-alkaline phosphatase method was used to measure the stained cells:total cell-number ratio (labelling index, Bonferroni adjustment). Real-time RT-PCR was performed on ONJ-affected and healthy jaw periodontal samples (n = 20 each) to quantitatively compare Msx-1, BMP-2, RANKL, and GAPDH mRNA levels.</p> <p>Results</p> <p>Semi-quantitative assessment of the ratio of stained cells showed decreased Msx-1 and RANKL and increased BMP-2/4 (all p < 0.05) expression in ONJ-adjacent periodontal tissue. ONJ tissue also exhibited decreased relative gene expression for Msx-1 (p < 0.03) and RANKL (p < 0.03) and increased BMP-2/4 expression (p < 0.02) compared to control.</p> <p>Conclusions</p> <p>These results explain the sclerotic and osteopetrotic changes of periodontal tissue following BP application and substantiate clinical findings of BP-related impaired remodeling specific to periodontal tissue. RANKL suppression substantiated the clinical finding of impaired bone remodelling in BP- and aRANKL-induced ONJ-affected bone structures. Msx-1 suppression in ONJ-adjacent periodontal tissue suggested a bisphosphonate-related impairment in cellular differentiation that occurred exclusively jaw remodelling. Further research on developmental biology-related unique features of jaw bone structures will help to elucidate pathologies restricted to maxillofacial tissue.</p

Autocorrelation of the Ground Vibrations Recorded by the SEIS‐InSight Seismometer on Mars

Since early February 2019, the SEIS (Seismic Experiment for Interior Structure) seismometer deployed at the surface of Mars in the framework of the InSight mission has been continuously recording the ground motion at Elysium Planitia. In this study, we take advantage of this exceptional data set to put constraints on the crustal properties of Mars using seismic interferometry (SI). To carry out this task, we first examine the continuous records from the very broadband seismometer. Several deterministic sources of environmental noise are identified and specific preprocessing strategies are presented to mitigate their influence. Applying the principles of SI to the single-station configuration of InSight, we compute, for each Sol and each hour of the martian day, the diagonal elements of the time-domain correlation tensor of random ambient vibrations recorded by SEIS. A similar computation is performed on the diffuse waveforms generated by more than a hundred Marsquakes. A careful signal- to-noise ratio analysis and an inter-comparison between the two datasets suggest that the results from SI are most reliable in a narrow frequency band around 2.4 Hz, where an amplification of both ambient vibrations and seismic events is observed. The average autocorrelation functions (ACFs) contain well identifiable seismic arrivals, that are very consistent between the two datasets. Interpreting the vertical and horizontal ACFs as, respectively, the P- and S- seismic reflectivity below InSight, we propose a simple stratified velocity model of the crust, which is mostly compatible with previous results from receiver function analysis. Our results are discussed and compared to recent works from the literature

The role of mechanotransduction versus hypoxia during simulated orthodontic compressive strain—an in vitro study of human periodontal ligament fibroblasts

During orthodontic tooth movement (OTM) mechanical forces trigger pseudo-inflammatory, osteoclastogenic and remodelling processes in the periodontal ligament (PDL) that are mediated by PDL fibroblasts via the expression of various signalling molecules. Thus far, it is unknown whether these processes are mainly induced by mechanical cellular deformation (mechanotransduction) or by concomitant hypoxic conditions via the compression of periodontal blood vessels. Human primary PDL fibroblasts were randomly seeded in conventional six-well cell culture plates with O-2-impermeable polystyrene membranes and in special plates with gas-permeable membranes (Lumox (R), Sarstedt), enabling the experimental separation of mechanotransducive and hypoxic effects that occur concomitantly during OTM. To simulate physiological orthodontic compressive forces, PDL fibroblasts were stimulated mechanically at 2 g.cm(-2) for 48 h after 24 h of pre-incubation. We quantified the cell viability by MTT assay, gene expression by quantitative real-time polymerase chain reaction (RT-qPCR) and protein expression by western blot/enzyme-linked immunosorbent assays (ELISA). In addition, PDL-fibroblast-mediated osteoclastogenesis (TRAP(+) cells) was measured in a 72-h coculture with RAW264.7 cells. The expression of HIF-1 alpha, COX-2, PGE2, VEGF, COL1A2, collagen and ALPL, and the RANKL/OPG ratios at the mRNA/protein levels during PDL-fibroblast-mediated osteoclastogenesis were significantly elevated by mechanical loading irrespective of the oxygen supply, whereas hypoxic conditions had no significant additional effects. The cellular-molecular mediation of OTM by PDL fibroblasts via the expression of various signalling molecules is expected to be predominantly controlled by the application of force (mechanotransduction), whereas hypoxic effects seem to play only a minor role. In the context of OTM, the hypoxic marker HIF-1 alpha does not appear to be primarily stabilized by a reduced O-2 supply but is rather stabilised mechanically