Nuclear Receptor Unfulfilled Regulates Axonal Guidance and Cell Identity of Drosophila Mushroom Body Neurons

Nuclear receptors (NRs) comprise a family of ligand-regulated transcription factors that control diverse critical biological processes including various aspects of brain development. Eighteen NR genes exist in the Drosophila genome. To explore their roles in brain development, we knocked down individual NRs through the development of the mushroom bodies (MBs) by targeted RNAi. Besides recapitulating the known MB phenotypes for three NRs, we found that unfulfilled (unf), an ortholog of human photoreceptor specific nuclear receptor (PNR), regulates axonal morphogenesis and neuronal subtype identity. The adult MBs develop through remodeling of γ neurons plus de-novo elaboration of both α′/β′ and α/β neurons. Notably, unf is largely dispensable for the initial elaboration of γ neurons, but plays an essential role in their re-extension of axons after pruning during early metamorphosis. The subsequently derived MB neuron types also require unf for extension of axons beyond the terminus of the pruned bundle. Tracing single axons revealed misrouting rather than simple truncation. Further, silencing unf in single-cell clones elicited misguidance of axons in otherwise unperturbed MBs. Such axon guidance defects may occur as MB neurons partially lose their subtype identity, as evidenced by suppression of various MB subtype markers in unf knockdown MBs. In sum, unf governs axonal morphogenesis of multiple MB neuron types, possibly through regulating neuronal subtype identity

A dislocation dynamics study of the strength of stacking fault tetrahedra. Part II: interactions with mixed and edge dislocations

In this paper we present the sequel to Part I and present a comprehensive dislocation dynamics study of the strength of stacking fault tetrahedra to mixed and edge dislocation glides in fcc Cu

Hybrid Elastic ARM&Cloud HPC Collaborative Platform for generic tasks

Compute-heavy workloads are currently run on Hybrid HPC structures using x86 CPUs and GPUs from Intel, AMD, or NVidia, which have extremely high energy and financial costs. However, thanks to the incredible progress made on CPUs and GPUs based on the ARM architecture and their ubiquity in today’s mobile devices, it’s possible to conceive of a low-cost solution for our world’s data processing needs. Every year ARM-based mobile devices become more powerful, efficient, and come in ever smaller packages with ever growing storage. At the same time, smartphones waste these capabilities at night while they’re charging. This represents billions of idle devices whose processing power is not being utilized. For that reason, the objective of this paper is to evaluate and develop a hybrid, distributed, scalable, and redundant platform that allows for the utilization of these idle devices through a cloud-based administration service. The system would allow for massive improvements in terms of efficiency and cost for com-pute-heavy workload. During the evaluation phase, we were able to establish savings in power and cost significant enough to justify exploring it as a serious alternative to traditional computing architectures.Instituto de Investigación en Informátic

A "kilonova" associated with short-duration gamma-ray burst 130603B

Short-duration gamma-ray bursts (SGRBs) are intense flashes of cosmic gamma-rays, lasting less than ~2 s, whose origin is one of the great unsolved questions of astrophysics today. While the favoured hypothesis for their production, a relativistic jet created by the merger of two compact stellar objects (specifically, two neutron stars, NS-NS, or a neutron star and a black hole, NS-BH), is supported by indirect evidence such as their host galaxy properties, unambiguous confirmation of the model is still lacking. Mergers of this kind are also expected to create significant quantities of neutron-rich radioactive species, whose decay should result in a faint transient in the days following the burst, a so-called "kilonova". Indeed, it is speculated that this mechanism may be the predominant source of stable r-process elements in the Universe. Recent calculations suggest much of the kilonova energy should appear in the near-infrared (nIR) due to the high optical opacity created by these heavy r-process elements. Here we report strong evidence for such an event accompanying SGRB 130603B. If this simplest interpretation of the data is correct, it provides (i) support for the compact object merger hypothesis of SGRBs, (ii) confirmation that such mergers are likely sites of significant r-process production and (iii) quite possibly an alternative, un-beamed electromagnetic signature of the most promising sources for direct detection of gravitational waves.Comment: preprint of paper appearing in Nature (3 Aug 2013

Mechanical activation of vinculin binding to talin locks talin in an unfolded conformation

The force-dependent interaction between talin and vinculin plays a crucial role in the initiation and growth of focal adhesions. Here we use magnetic tweezers to characterise the mechano-sensitive compact N-terminal region of the talin rod, and show that the three helical bundles R1-R3 in this region unfold in three distinct steps consistent with the domains unfolding independently. Mechanical stretching of talin R1-R3 enhances its binding to vinculin and vinculin binding inhibits talin refolding after force is released. Mutations that stabilize R3 identify it as the initial mechano-sensing domain in talin, unfolding at ~5 pN, suggesting that 5 pN is the force threshold for vinculin binding and adhesion progression

Quantifying Inactive Lithium in Lithium Metal Batteries

Inactive lithium (Li) formation is the immediate cause of capacity loss and catastrophic failure of Li metal batteries. However, the chemical component and the atomic level structure of inactive Li have rarely been studied due to the lack of effective diagnosis tools to accurately differentiate and quantify Li+ in solid electrolyte interphase (SEI) components and the electrically isolated unreacted metallic Li0, which together comprise the inactive Li. Here, by introducing a new analytical method, Titration Gas Chromatography (TGC), we can accurately quantify the contribution from metallic Li0 to the total amount of inactive Li. We uncover that the Li0, rather than the electrochemically formed SEI, dominates the inactive Li and capacity loss. Using cryogenic electron microscopies to further study the microstructure and nanostructure of inactive Li, we find that the Li0 is surrounded by insulating SEI, losing the electronic conductive pathway to the bulk electrode. Coupling the measurements of the Li0 global content to observations of its local atomic structure, we reveal the formation mechanism of inactive Li in different types of electrolytes, and identify the true underlying cause of low Coulombic efficiency in Li metal deposition and stripping. We ultimately propose strategies to enable the highly efficient Li deposition and stripping to enable Li metal anode for next generation high energy batteries

Four-nucleon contact interactions from holographic QCD

We calculate the low energy constants of four-nucleon interactions in an effective chiral Lagrangian in holographic QCD. We start with a D4-D8 model to obtain meson-nucleon interactions and then integrate out massive mesons to obtain the four-nucleon interactions in 4D. We end up with two low energy constants at the leading order and seven of them at the next leading order, which is consistent with the effective chiral Lagrangian. The values of the low energy constants are evaluated with the first five Kaluza-Klein resonances.Comment: 28 page

Flexible graph matching and graph edit distance using answer set programming

The graph isomorphism, subgraph isomorphism, and graph edit distance problems are combinatorial problems with many applications. Heuristic exact and approximate algorithms for each of these problems have been developed for different kinds of graphs: directed, undirected, labeled, etc. However, additional work is often needed to adapt such algorithms to different classes of graphs, for example to accommodate both labels and property annotations on nodes and edges. In this paper, we propose an approach based on answer set programming. We show how each of these problems can be defined for a general class of property graphs with directed edges, and labels and key-value properties annotating both nodes and edges. We evaluate this approach on a variety of synthetic and realistic graphs, demonstrating that it is feasible as a rapid prototyping approach.Comment: To appear, PADL 202