Emerging trends in the photodynamic inactivation (PDI) applied to the food decontamination

The food and drink manufacturing industry is constantly seeking for alternative sanitation and disinfection systems that may achieve the same antimicrobial efficiency of conventional chemical sanitisers and at the same time be convenient in terms of energy and water savings. A candidate technology for this purpose is the use of light in combination with photosensitisers (PS) to generate a bioactive effect against microbial agents in a process defined as photodynamic inactivation (PDI). This technology can be applied to the food processing of different food matrices to reduce the microbial load of foodborne pathogens such as bacteria, fungi, viruses and protozoa. Also, the PDI can be exploited to increase the shelf-life period of food by inactivation of spoiling microbes. This review analyses new developments in the last five years for PDI systems applied to the food decontamination from foodborne pathogens. The photosensitisation mechanisms and methods are reported to introduce the applied technology against microbial targets in food matrices. Recent blue light emitting diodes (LED) lamp systems for the PDI mediated by endogenous PS are discussed as well PDI technologies with the use of exogenous PS from plant sources such as curcumin and porphyrin-based molecules. The updated overview of the most recent developments in the PDI technology both in wavelengths and employed PS will provide further points of analysis for the advancement of the research on new competitive and effective disinfection systems in the food industry

Philippines-Japan Economic Partnership Agreement (PJEPA): An Initial Ex-post Review

While it is too early to undertake rigorous quantitative analysis on the impact of the Philippines-Japan Economic Partnership Agreement (PJEPA) on the Philippines, this initial ex-post study concludes that based on several key indicators the country has not suffered major adjustment costs because of the PJEPA. There is also evidence to show that the Philippines was able to secure concessions comparable to other partner countries of Japan in similar EPAs. The study echoes earlier research that indicated the short-run impact of the PJEPA on GDP would be small but the dynamic benefits could be very significant. On the whole, indicators are positive, although more could be done especially in terms of labor protection, attracting Japanese investments, and taking advantage of the cooperation elements of the agreement

Constant Value Added Share Analysis:A Novel Trade Decomposition Technique with an Application to the Philippines

This article provides a novel approach to the traditional constant market share (CMS) analysis recognizing the importance of global production networks and fragmentation that necessitate using trade in value added (TiVA) rather than gross trade. We discuss how the components of a CMS analysis need to be (re)interpreted and apply our Constant Value Added Share (CVAS) analysis to the Philippines in the years 1995–2020 using the latest TiVA 2023 dataset (released in November 2023) and compare CMS to CVAS in order to facilitate understanding the contribution of the novel approach. The CVAS analysis finds that while Philippine and world Value Added grew at par, the country lost competitiveness. Traditional CMS suggests a smaller loss of competitiveness. Our approach also identifies specific sectoral weaknesses (i.e., computer and electronics exports) and emerging strengths (technology-related business services) that are unclear in CMS analysis. We argue that Constant Value Added Share analysis is useful for assessing the global value chain performance of other developing countries as well

Evaluation of the energy utilization index in sheep milk cooling systems

The energy consumption of sheep milk cooling systems (MCSs) was quantified in this study to provide original information filling a literature gap on the impact of sheep milk cooling on the energy and economic balance in dairy farms. Performance and energy monitoring tests were conducted simultaneously on 22 MCSs in Sardinia (Italy). The results determined the cooling time as a function of the performance class and number of milkings. The Energy Utilization Index (EUI) was applied to measure the energy required to cool down the milk and estimate the incidence on its price. The average EUI was 1.76 kWh 100 L−1 for two-milkings and 2.43 kWh 100 L−1 for four-milkings MCSs, whereas the CO2 emissions ranged from 998 to 1378 g CO2 100 L−1 for two- and four-milkings MCSs, respectively. The estimated energy consumption for the storage of refrigerated sheep milk was 0.12 kWh 100 L−1. The malfunctioning MCSs averagely consumed 31% more energy than regular systems. The energy cost for cooling accounted for 0.61% on the current sheep milk price in Italy. Based on the analysis, the reported EUI values can be used as a preliminary indicator of the regular operation of MCSs

Increasing the agricultural sustainability of closed agrivoltaic systems with the integration of vertical farming: A case study on baby-leaf lettuce

The photovoltaic (PV) greenhouses are closed agrivoltaic (CA) systems that allow the production of energy and food on the same land, but may result in a yield reduction when the shading of the PV panels is excessive. Adopting innovative cropping systems can increase the yield of the CA area, generating a more productive and sustainable agrosystem. In this case study we quantified the increase of land productivity derived from the integration of an experimental vertical farm (VF) for baby leaf lettuce inside a pre-existing commercial CA. The mixed system increased the yield by 13 times compared to the CA and the average LER was 1.31, but only 12 % of the energy consumption was covered by the CA energy. To achieve the energy self-sufficiency and avoid the related CO2 emissions, the VF area should not exceed 7–18 % of the CA area, depending on the PV energy yield and the daily light integral (DLI) of the LED lighting, meaning a land consumption from 5 to 14 times higher than the VF area. The support of the PV energy was essential for the profitability of the VFCA. Design features and solutions were proposed to increase the agronomic and economic sustainability of the VFCA. The VFs can be considered a possible answer for the reconversion of the actual underutilized CAs with high PV cover ratios into productive and efficient cropping systems, but a trade-off between energy production and land consumption should be identified to ensure an acceptable environmental sustainability of the mixed system

Physiological Responses to Drought Stress in Jatropha curcas Seedlings

Aims: The aim of the study was to investigate the physiological mechanisms of Jatropha curcas seedlings exposed to drought and the possible influence of seedling age. Study Design: A pot experiment was carried out using a completely randomized design with two seedling ages (2- and 3-month-old seedlings), two treatments per age (Watered: fully irrigated, and Unwatered: Not irrigated), six replicates (24 pots). Place and Duration of Study: The experiment was performed in a greenhouse facility located at the Experimental Station “Mauro Deidda” (Department of Agriculture of University of Sassari) at Ottava (Sassari, Italy) between June and September 2011. Methodology: To investigate the responses of 2- and 3-month-old J. curcas seedlings exposed to drought stress on 4th, 8th, 12th, 19th, and 26th day from treatment’s beginning, leaf and soil water content, biometric, gas exchange, and chlorophyll a fluorescence measurements were performed; on 26th day from treatment’s beginning, biometric destructive measurements were carried out. Results: Results support the hypothesis that J. curcas is appropriate to be cultivated in areas with limited water availability or prolonged periods of drought and highlight that mechanisms of drought response are highly influenced by seedling age. J. curcas seedlings maintained a good leaf water status by means of an effective stomatal closure, associated with a reduced aboveground growth and an increased root:shoot ratio. Under drought stress, 2-month seedlings showed a higher allocation of resources to roots compared to 3-month seedlings. Drought resulted in more detrimental effects on the photosynthetic response of 3-month seedlings, inducing the reduction of stomata conductance and the loss of photosystem II integrity. 2-month seedlings were instead able to activate mechanisms of drought tolerance through the activation of excess energy dissipation mechanisms. Conclusion: In the early stage of crop establishment, the transplanting of J. curcas 2-month seedlings proved to be more effective in order to avoid water stress related consequences

Agricultural sustainability estimation of the European photovoltaic greenhouses

The integration of the photovoltaic (PV) energy in the greenhouse farm has raised concerns on the agricultural sustainability of this specific agrosystem in terms of crop planning and management, due to the shading cast by the PV panels on the canopy. The PV greenhouse (PVG) can be classified on the basis of the PV cover ratio (PVR), that is the ratio of the projected area of PV panels to the ground and the total greenhouse area. In this paper, we estimated the yield of 14 greenhouse horticultural and floricultural crops inside four commercial PVG types spread in southern Europe, with PVR ranging from 25 to 100%. The aim of the work is to identify the PVG types suitable for the cultivation of the considered species, based on the best trade-off between PV shading and crop production. The daily light integral (DLI) was used to compare the light scenarios inside the PVGs to the crop light requirements, and estimate the potential yield. The structures with a PVR of 25% were compatible with the cultivation of all considered species, including the high light demanding ones (tomato, cucumber, sweet pepper), with an estimated negligible or limited yield reduction (below 25%). The medium light species (such as asparagus) with an optimal DLI lower than 17 mol m−2 d−1 and low light crops can be cultivated inside PVGs with a PVR up to 60%. Only low light demanding floricultural species with an optimal DLI lower than 10 mol m−2 d−1, such as poinsettia, kalanchoe and dracaena, were compatible inside PVGs with a PVR up to 100%. Innovative cropping systems should be considered to overcome the penalizing light scenarios of the PVGs with high PVR, also implementing LED supplementary lighting. This paper contributes to identify the sustainable PVG types for the chosen species and the alternative crop managements in terms of transplantation period and precision agriculture techniques, aimed at increasing the crop productivity and adaptability inside the PVG agrosystems

Flow dynamics in a model of a dilated thoracic aorta prior to and following prosthetic replacement

We numerically investigate the flow dynamics in a model of a dilated thoracic aorta, and compare the flow features with the case of a prosthetic replacement in its ascending part. The flow is characterized by an inlet jet which impacts the aortic walls and sweeps toward the aortic arch. Secondary flows generated by the transvalvular jet evolve downstream into a helical flow. The small curvature radius at the end of the aortic arch induces flow separation and vortex shedding in the initial part of the descending aorta, during the systole. The implantation of a prosthesis determines several modifications in the global and local flow patterns. An increase of the pulse wave velocity in the aorta leads to larger pressures inside the vessel, due to the geometrical and rigidity modifications. The sweeping jet is more aligned along the axial direction and propagates faster along the aortic arch. Consequently, a stronger separation of the flow downstream of the aortic arch is observed. By also exploiting manifold analysis, we identify regions characterized by near-wall disordered flows which may present intense accumulation and drop of concentration of biochemicals. These regions are localized downstream of the prosthetic replacement, in the aortic arch, and may be more prone to a new emergence of vessel dilation

The Conserved nhaAR Operon Is Drastically Divergent between B2 and Non-B2 Escherichia coli and Is Involved in Extra-Intestinal Virulence

The Escherichia coli species is divided in phylogenetic groups that differ in their virulence and commensal distribution. Strains belonging to the B2 group are involved in extra-intestinal pathologies but also appear to be more prevalent as commensals among human occidental populations. To investigate the genetic specificities of B2 sub-group, we used 128 sequenced genomes and identified genes of the core genome that showed marked difference between B2 and non-B2 genomes. We focused on the gene and its surrounding region with the strongest divergence between B2 and non-B2, the antiporter gene nhaA. This gene is part of the nhaAR operon, which is in the core genome but flanked by mobile regions, and is involved in growth at high pH and high sodium concentrations. Consistently, we found that a panel of non-B2 strains grew faster than B2 at high pH and high sodium concentrations. However, we could not identify differences in expression of the nhaAR operon using fluorescence reporter plasmids. Furthermore, the operon deletion had no differential impact between B2 and non-B2 strains, and did not result in a fitness modification in a murine model of gut colonization. Nevertheless, sequence analysis and experiments in a murine model of septicemia revealed that recombination in nhaA among B2 strains was observed in strains with low virulence. Finally, nhaA and nhaAR operon deletions drastically decreased virulence in one B2 strain. This effect of nhaAR deletion appeared to be stronger than deletion of all pathogenicity islands. Thus, a population genetic approach allowed us to identify an operon in the core genome without strong effect in commensalism but with an important role in extra-intestinal virulence, a landmark of the B2 strains

Attitudes of healthcare personnel towards vaccinations before and during the covid-19 pandemic

Vaccines constitute highly effective tools for controlling and eliminating vaccine-preventable diseases (VPDs) and are assessed to avert between two to three million deaths per year globally. Healthcare personnel (HCP) constitute a priority group for several vaccinations. However, studies indicate significant rates of vaccine hesitancy among them and, therefore, of acceptance of vaccination recommendations. This cross-sectional study was conducted in a university hospital in Southern Italy to assess the knowledge and attitudes of HCP about VPDs before and during the COVID-19 pandemic, estimate their intention to get vaccinated against COVID-19, and search for determinants that may influence their choice. A self-administered questionnaire was used. HCP improved their knowledge about VPDs and were more favorable to vaccinations in September\u2013December 2020 compared to January\u2013December 2019. Overall, 75% of respondents would get a COVID-19 vaccine. Our findings indicate a potential role of the ongoing COVID-19 pandemic on Italian HCP\u2019s knowledge and attitudes towards vaccines