Effectiveness Of Rotatory And Reciprocating Movements In Root Canal Filling Material Removal

The purpose of this study was to compare the efficacy of reciprocating and rotary techniques for removing gutta-percha and sealer from root canals. Forty straight and oval single-rooted premolars were prepared up to size 30, filled with gutta-percha and sealer, and then randomly allocated to two experimental retreatment groups: ProTaper Retreatment System (PTRS) and WaveOne System (WS). Procedural errors, time of retreatment and apically extruded material were recorded for all the roots. The roots were radiographed after retreatment. The percentage of residual material was calculated using image analysis software. The data were analyzed by Kolmogorov-Smirnov and t tests, with a significance level set at 5%. No system completely removed the root filling material from the root canal. No significant differences were observed between the systems, in terms of residual filling material in any tested third (p > 0.05). WS was faster in removing filling material than PTRS (p < 0.05). Extrusion was observed in 4 cases in PTRS and in 5 cases in WS. No procedural errors were observed in either group. It can be concluded that although no differences were observed in the efficacy of PTRS and WS for removing root filling material, WS was faster than PTRS.29

Black Soldier Fly (Hermetia illucens) pre-pupae meal as a fish meal replacement in diets for European seabass (Dicentrarchus labrax)

[EN] A feeding trial was carried out to assess the effect of partially replacing fish meal (FM) by Black soldier fly pre-pupae meal (HM) in diets for European seabass Dicentrarchus labrax juveniles. A FM-based diet was used as a control and three other diets were formulated to include 6.5%, 13%, and 19.5% of HM, replacing 15%, 30% and 45% of FM respectively. Each diet was fed to triplicate groups of fish (initial weight: 50 g) for 62 days. At the end of the trial, there were no differences among groups in growth performance or feed utilization. Plasma metabolic profiles also remained unaffected, except that plasma cholesterol was reduced with dietary HM inclusion. The apparent digestibility coefficients (ADC) of protein, lipids, dry matter, organic matter, and energy were generally high, and not affected by the dietary treatment. The ADC of arginine, histidine, and valine were higher in HM diets when compared to the control. Amylase and protease activities were not affected by dietary HM, while lipase activity was lower in HM 6.5 diets than in the control and HM 19.5 diets. In conclusion, up to 19.5% of HM, corresponding to 22.5% of total dietary protein, may successfully replace FM in diets for juvenile European seabass, without adverse effects on growth performance, feed utilization or digestibility.This research was partially supported by the Strategic Funding UID/Multi/04423/2013 through national funds provided by FCT - Foundation for Science and Technology and European Regional Development Fund (ERDF), in the framework of the programme PT2020. We would like to express our thanks to P. Correia for assistance during the growth and digestibility trial. Magalhaes, R. was supported by a VEGICOR-AMINOAQUA project grant (ref. 31-03-05-FEP-43)Magalhães, R.; Sanchez-Lopez, A.; Leal, RS.; Martínez-Llorens, S.; Oliva-Teles, A.; Peres, H. (2017). Black Soldier Fly (Hermetia illucens) pre-pupae meal as a fish meal replacement in diets for European seabass (Dicentrarchus labrax). Aquaculture. 476:79-85. https://doi.org/10.1016/j.aquaculture.2017.04.021S798547

Influência dos resíduos da colheita da floresta de acácia-negra (Acacia mearnsii De Wild) sobre as perdas de água e solo.

This work was carried out at Federal University of Santa Maria campus, Rio Grande do Sul, Brazil, with the objective to evaluate the influence of residues management system of an black wattle reforestation on water and soil losses from natural rainfall. The treatments were: T1 - shallow clear cutting burned; T2 - cut and deposition of the residues and T3 - reforestation of black wattle. The treatment T1 had significantly greater water losses than T2, which also had significantly greater water losses than T3. The precipitation during the studied period was 1,384 mm, with anerosivity index (EI30) of 7,474 MJ mm ha-1 h-1. The  soil  losses  evidenced  significantly differences between T1 and the other two treatments T2 and T3, which reduced the soil losses in 99,6% and 99,9%, respectively, in relation to T1.Este trabalho foi conduzido no Campus da Universidade Federal de Santa Maria, Rio Grande do Sul, com o objetivo de avaliar a influência do sistema de manejo dos resíduos da exploração de um reflorestamento de acácia-negra sobre as perdas de água e solo provocadas por chuva natural. Os tratamentos consistiram: T1 - corte raso e queimada dos resíduos; T2 - corte raso e deposição dos resíduos; e T3 - reflorestamento de acácia-negra. O tratamento T1 perdeu significativamente mais água que o tratamento T2, o qual também perdeu significativamente mais água que o tratamento T3. A precipitação durante o período estudado foi de 1.384 mm, com índice de erosividade de chuva (EI30) de 7.474 MJ mm ha-1 h-1. As perdas de solo evidenciaram diferenças significativas entre T1 e os outros dois tratamentos T2 e T3, os quais reduziram as perdas de solo em 99,6% e 99,9%, respectivamente, em relação a T1

Surgical alternative for treatment of vertical root fracture: A case report

Vertical root fracture (VRF) has been a great challenge in dentistry; most fractures often result in tooth extraction. Inflammation of tissues around the fractured root is the main reason for tooth extraction. Based on the strategic importance of some fractured teeth, treatment may be necessary and often complicated. However, performing a proper repair or even splinting the fractured segments may result in tooth preservation. Accordingly, in this case we report a new method for fractured tooth preservation. The surgical exposition of the fracture tooth was carried out through the radicular portion of the element via ultrasonic preparation, filling with composed resin and a synthetic hydroxyapatite graft. All these were performed around the tooth which received five sections of low-power laser. The patient was followed for two years with no signs or symptoms of inflammation and gingival recession. In conclusion, the used treatment protocol could be considered as a promising approach for VRF treatment, especially in cases where there is advanced or moderate bone loss in the surrounding sites of the fractured tooth

Uma proposta para se agregar valor energético a resíduos sólidos de qualquer tipo de madeira / A proposal to add energy value to solid residues of any type of wood

É sabido que o aumento de densidade energética da madeira pode também ser obtido a partir da produção de briquetes de resíduos dessa biomassa. Em função da elevada compactação desses resíduos, os briquetes obtidos a partir disso apresentam valores de densidade energética de duas a três vezes o valor obtido para madeira e até sete vezes a do próprio resíduo. O principal objetivo desse trabalho foi investigar em caráter exploratório, o efeito combinado da pressão e adição de sólidos carbonáceos a estrutura da madeira, no comportamento das propriedades energéticas, em especial, a densidade energética deste material. Diferentes resíduos de madeira de origem desconhecida na forma de galhos colhidos no campus da UFR foram usinados com dimensões próximas àquelas compactadas em diferentes pressões. Os resíduos de pó obtidos a partir do torneamento destes galhos com tamanho de partícula ≤ 0,85 mm foram compactados em pressões de até 300 MPa. As amostras usinadas e os briquetes produzidos em diferentes pressões foram caracterizados via análise imediata, determinação do poder calorífico e medidas de densidade a granel e energética. Os resultados mostraram que a adição de sólidos carbonáceos à estrutura da madeira influenciou significativamente no comportamento das densidades a granel e energética deste material. Comparando-se os resultados foi possível identificar nas amostras saturadas com sólidos carbonáceos um aumento percentual na densidade energética de até 49,4%

Evaluation of the adsorption process of remazol black b dye in liquid effluents by green coconut mesocarp

The textile industry is economically important for the development of Brazil. However, the generated effluents from this activity present a complex molecular structure that gives a recalcitrant characteristic in its residuary waters. Due to this reality, it is necessary to develop viable technologies that remove those compounds, which are mostly dyes. In this study, the green coconut mesocarp was used to remove Remazol Black B dye of an aqueous solution using the adsorption technique. To perform the experiments, the green coconut mesocarp was dried at 60ºC, ground in a grinding mill, sifted, washed with distilled water and immersed in albumin for 24 hours. The process of adsorption was optimized in a batch mode, containing 0.5 g of adsorbent and 25 mL of synthetic solution of 10 mg L -1, at 30ºC, pH 4.0, stirring speed of 700 rpm, and granulometry G ￡ 0.149 mm. Afterwards, the influence of the pH of the solution, adsorbent granulometry and stirring speed were evaluated by means of factorial planning. Experiments of equilibrium and kinetics of adsorption were conducted in Erlenmeyer. Results indicated that among the main effects, the pH and the adsorbent granulometry significantly influenced the process of adsorption. Besides, the adsorption equilibrium was reached in 60 minutes of contact. The Langmuir model was adjusted to experimental results (R 2=0.9953) being obtained a maximum capacity of adsorptionof 2.93 mgg-1 and equilibrium constant of 0.26 Lmg-1. With the obtained results it could be concluded that the green coconut mesocarp could be a viable alternative for the treatment of textile effluents

Comprehensive molecular landscape of cetuximab resistance in head and neck cancer cell lines

Cetuximab is the sole anti-EGFR monoclonal antibody that is FDA approved to treat head and neck squamous cell carcinoma (HNSCC). However, no predictive biomarkers of cetuximab response are known for HNSCC. Herein, we address the molecular mechanisms underlying cetuximab resistance in an in vitro model. We established a cetuximab resistant model (FaDu), using increased cetuximab concentrations for more than eight months. The resistance and parental cells were evaluated for cell viability and functional assays. Protein expression was analyzed by Western blot and human cell surface panel by lyoplate. The mutational profile and copy number alterations (CNA) were analyzed using whole-exome sequencing (WES) and the NanoString platform. FaDu resistant clones exhibited at least two-fold higher IC50 compared to the parental cell line. WES showed relevant mutations in several cancer-related genes, and the comparative mRNA expression analysis showed 36 differentially expressed genes associated with EGFR tyrosine kinase inhibitors resistance, RAS, MAPK, and mTOR signaling. Importantly, we observed that overexpression of KRAS, RhoA, and CD44 was associated with cetuximab resistance. Protein analysis revealed EGFR phosphorylation inhibition and mTOR increase in resistant cells. Moreover, the resistant cell line demonstrated an aggressive phenotype with a significant increase in adhesion, the number of colonies, and migration rates. Overall, we identified several molecular alterations in the cetuximab resistant cell line that may constitute novel biomarkers of cetuximab response such as mTOR and RhoA overexpression. These findings indicate new strategies to overcome anti-EGFR resistance in HNSCC.This work was supported by Barretos Cancer Hospital and the Public Ministry of Labor Campinas (Research, Prevention, and Education of Occupational Cancer) in Campinas, Brazil, CAPESDFATD (88887.137283/2017-00). INFG is the recipient of a FAPESP Ph.D. fellowship (2017/22305-9)

Vocal repertoire of Scinax v-signatus (Lutz 1968) (Anura, Hylidae) and comments on bioacoustical synapomorphies for Scinax perpusillus species group

Herein we describe the vocal repertoire of Scinax v-signatus, compare it to the other species belonging to the S. perpusillus species group and discuss the bioacoustical synapomorphies proposed for the group. We recorded an advertisement call and an aggressive call of S. v-signatus. The advertisement call is similar to the one described for others species of the Scinax perpusillus group. Similarly to Scinax cosenzai, the first note in the advertisement call of S. v-signatus is the longest. The aggressive call was preceded by a series of advertisement calls. We suggest that bioacoustical parameters proposed as synapomorphies for the Scinax perpusillus group are not valid, as they are also observed in species belonging to other groups within the genus

Serum Levels of Infliximab and Anti-Infliximab Antibodies in Brazilian Patients with Crohn’s Disease

OBJECTIVES: The aim of this study was to evaluate the quantitative serum level of infliximab (IFX) as well as the detection of anti-infliximab antibodies (ATIs) in patients with Crohn’s disease (CD). METHOD: Forty patients with CD under treatment at a tertiary center in southeastern Brazil were evaluated. Their use of infliximab was continuous and regular. We analyzed and compared the differences in the IFX and ATI levels between the patients with active CD (CDA) and those with CD in remission (CDR). RESULTS: There was no difference in the IFX level between the CDA and CDR groups (p40.05). Eighty percent of all patients had IFX levels above the therapeutic concentration (6-10 mg/mL). Two (9%) of the 22 patients with active disease and four (22.2%) of the 18 patients in remission had undetectable levels of IFX. Four (66.6%) of the six patients with undetectable levels of IFX had positive ATI levels; three of these patients were in remission, and one had active disease. In addition, the other two patients with undetectable levels of IFX presented ATI levels close to positivity (2.7 and 2.8 AU/ml). None of the patients with therapeutic or supratherapeutic IFX levels had positive ATI levels. CONCLUSIONS: The undetectable levels of IFX correlated with the detection of ATIs, which was independent of disease activity. Immunogenicity was not the main factor for the loss of response to IFX in our study, and the majority of patients in both groups (CDA and CDR) had supratherapeutic levels of IFX