2,2′-Azobis (2-Amidinopropane) Dihydrochloride Is a Useful Tool to Impair Lung Function in Rats

Recently, several studies have reported that respiratory disease may be associated with an increased production of free radicals. In this context, 2,2’-azobis (2-amidinopropane) dihydrochloride (AAPH) is a free radical-generating compound widely used to mimic the oxidative stress state. We aimed to investigate whether AAPH can generate lung functional, inflammatory, histological and biochemical impairments in the lung. Wistar rats were divided into five groups and instilled with saline solution (714 µL/kg, CTRL group) or different amounts of AAPH (25, 50, 100 and 200 mg/kg, 714 µL/kg, AAPH groups). Seventy-two hours later the animals were anesthetized, paralyzed, intubated and static elastance (Est), viscoelastic component of elastance (ΔE), resistive (ΔP1) and viscoelastic (ΔP2) pressures were measured. Oxidative damage, inflammatory markers and lung morphometry were analyzed. ΔP1 and Est were significantly higher in AAPH100 and AAPH200 than in the other groups. The bronchoconstriction indexes were larger in AAPH groups than in CTRL. The area occupied by collagen and elastic fibers, polymorpho- and mononuclear cells, malondialdehyde and carbonyl groups levels were significantly higher in AAPH200 than in CTRL. In comparison to CTRL, AAPH200 showed significant decrease and increase in the activities of superoxide dismutase and catalase, respectively. AAPH augmented the release of pro-inflammatory cytokines IL-1β, IL-6 e TNF-α. Hence, exposure to AAPH caused significant inflammatory alterations and redox imbalance accompanied by altered lung mechanics and histology. Furthermore, we disclosed that exposure to AAPH may represent a useful in vivo tool to trigger lung lesions

CONHECIMENTO DE ESTUDANTES DE ENFERMAGEM SOBRE PARADA CARDIORRESPIRATÓRIA: ESTUDO TRANSVERSAL

Objetivo: avaliar o conhecimento de estudantes de enfermagem sobre o atendimento ao paciente em parada cardiorrespiratória. Método: estudo transversal com abordagem quantitativa, realizado com 136 acadêmicos do curso de enfermagem de uma instituição de ensino superior. Os dados foram coletados por meio de instrumento semiestruturado. A análise efetivou-se por meio da estatística descritiva e inferencial. Resultados: os discentes que se autoavaliaram positivamente, mostraram um bom nível de conhecimento sobre a temática (r=0,29; p=0,001), assim como aqueles que haviam cursado a disciplina de primeiros socorros em períodos anteriores (p=0,012). As questões nas quais obtiveram erro foram aquelas a respeito das vias de administração da droga e suporte ventilatório em pacientes não intubados. Conclusão: os discentes de enfermagem demonstraram um nível adequado de compreensão dos protocolos sobre o atendimento ao paciente em parada cardiorrespiratória e possuíam arcabouço teórico obtido na disciplina de primeiros socorros, bem como em cursos de aperfeiçoamento. Descritores: Enfermagem. Estudantes de Enfermagem. Conhecimento. Parada Cardíaca.

Relative growth of carcass tissues of goat kids from five breed Types finished on pasture or feedlot

he aim of this study was to evaluate the effect of cross breeding, finishing system, and gender on the relative growth of carcass tissues of dairy kids. Seventy eight kids (39 male and 39 female) from five breed types were used: Alpine; ½ Boer + ½ Alpine (½ BA); ½ Nubian + ½ Alpine (½ ANA); ¾ Boer + ¼ Alpine (¾ BA); and ½ Nubian + ¼ Boer + ¼ Alpine (TC). Kids were distributed into two finishing systems: in pasture with doe (FS1) and weaned in feedlot (FS2). Kids were slaughtered at a mean age of 128.4 ± 7.9 days and mean live weight of 22.07 kg. The mean weight of half carcasses was 5.09 kg. To determine allometric growth, we used the exponential equation Y= aXb. In the half carcass, muscle tissue showed comparatively early growth in group ½ BA, whereas fat tissue of animals in FS1 had relatively late growth. Females exhibited early growth of muscle tissue, while in males this tissue was intermediate. The ½ BA first-cross improved carcass characteristics by enhancing the growth of muscle tissue

Tacit Knowledge: Characteristics in nursing practice

Introducción: El conocimiento tácito puede definirse como aquel que se utiliza de forma intuitiva e inconsciente, y que se adquiere mediante la propia experiencia, caracterizándose por ser personal y contextual. Términos como «intuición», «saber cómo» y «conocimiento implícito» se han utilizado para describirlo. Diferentes disciplinas en el ámbito de la gestión y la salud han estudiado el conocimiento tácito y lo han identificado como una potente herramienta para la creación de conocimiento y la toma de decisiones clínica. El objetivo de esta revisión fue analizar la definición y las características que componen el conocimiento tácito, así como determinar el rol que desempeña en la disciplina enfermera. Método: Se realizó una revisión integrativa de la literatura publicada hasta noviembre de 2016 en las bases de datos CUIDEN, SciELO, PubMed, Cochrane y CINAHL. La síntesis y la interpretación de los datos fue realizada por dos investigadores mediante análisis del contenido. Resultados: De los 819 artículos localizados, se seleccionaron 35 sobre el conocimiento tácito y la disciplina enfermera. No existe consenso en la denominación y la interpretación de los resultados en conocimiento tácito. Las principales características del conocimiento tácito son su carácter personal y social, siendo utilizado a partir de una estructura mental organizada (mindline). Esta estructura está relacionada con el uso del conocimiento tácito en la toma de decisiones clínicas. Conclusiones: Los estudios realizados sobre conocimiento tácito y la disciplina enfermera aportan diversas perspectivas sin profundizar en la materia. Se plantea la elaboración de un marco de relaciones que clarifique los conceptos implicados y su papel en la gestión del conocimiento enfermero.Introduction: Tacit knowledge can be defined as knowledge which is used intuitively and unconsciously, which is acquired through one’s experience, characterized by being personal and contextual. Some terms such as ‘intuition’, ‘know how’ and ‘implicit knowledge’ have been used to describe tacit knowledge. Different disciplines in the fields of management or health have studied tacit knowledge, identifying it as a powerful tool to create knowledge and clinical decision-making. The aim of this review is to analyse the definition and characteristics that make up tacit knowledge and determine the role it plays in the nursing discipline. Methods: An integrative review was undertaken of the literature published up to November 2016 in the databases CUIDEN, SciELO, PubMed, Cochrane and CINAHL. The synthesis and interpretation of the data was performed by two researchers through content analysis. Results: From a total of 819 articles located, 35 articles on tacit knowledge and nursing were chosen. There is no consensus on the name and description of results in tacit knowledge. The main characteristics of tacit knowledge have a personal and social character, which is used from an organised mental structure, called mindline. This structure relates to the use of tacit knowledge on clinical decision-making. Conclusions: Previous studies on tacit knowledge and nursing provide the nursing community with perspectives without going into depth. The production of a framework is suggested, as it would clarify implied concepts and its role on the management of nursing knowledge

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

The psychological science accelerator’s COVID-19 rapid-response dataset

In response to the COVID-19 pandemic, the Psychological Science Accelerator coordinated three large-scale psychological studies to examine the effects of loss-gain framing, cognitive reappraisals, and autonomy framing manipulations on behavioral intentions and affective measures. The data collected (April to October 2020) included specific measures for each experimental study, a general questionnaire examining health prevention behaviors and COVID-19 experience, geographical and cultural context characterization, and demographic information for each participant. Each participant started the study with the same general questions and then was randomized to complete either one longer experiment or two shorter experiments. Data were provided by 73,223 participants with varying completion rates. Participants completed the survey from 111 geopolitical regions in 44 unique languages/dialects. The anonymized dataset described here is provided in both raw and processed formats to facilitate re-use and further analyses. The dataset offers secondary analytic opportunities to explore coping, framing, and self-determination across a diverse, global sample obtained at the onset of the COVID-19 pandemic, which can be merged with other time-sampled or geographic data

A global experiment on motivating social distancing during the COVID-19 pandemic

Finding communication strategies that effectively motivate social distancing continues to be a global public health priority during the COVID-19 pandemic. This cross-country, preregistered experiment (n = 25,718 from 89 countries) tested hypotheses concerning generalizable positive and negative outcomes of social distancing messages that promoted personal agency and reflective choices (i.e., an autonomy-supportive message) or were restrictive and shaming (i.e., a controlling message) compared with no message at all. Results partially supported experimental hypotheses in that the controlling message increased controlled motivation (a poorly internalized form of motivation relying on shame, guilt, and fear of social consequences) relative to no message. On the other hand, the autonomy-supportive message lowered feelings of defiance compared with the controlling message, but the controlling message did not differ from receiving no message at all. Unexpectedly, messages did not influence autonomous motivation (a highly internalized form of motivation relying on one’s core values) or behavioral intentions. Results supported hypothesized associations between people’s existing autonomous and controlled motivations and self-reported behavioral intentions to engage in social distancing. Controlled motivation was associated with more defiance and less long-term behavioral intention to engage in social distancing, whereas autonomous motivation was associated with less defiance and more short- and long-term intentions to social distance. Overall, this work highlights the potential harm of using shaming and pressuring language in public health communication, with implications for the current and future global health challenges

Clinical complexity and impact of the ABC (Atrial fibrillation Better Care) pathway in patients with atrial fibrillation: a report from the ESC-EHRA EURObservational Research Programme in AF General Long-Term Registry

Background: Clinical complexity is increasingly prevalent among patients with atrial fibrillation (AF). The ‘Atrial fibrillation Better Care’ (ABC) pathway approach has been proposed to streamline a more holistic and integrated approach to AF care; however, there are limited data on its usefulness among clinically complex patients. We aim to determine the impact of ABC pathway in a contemporary cohort of clinically complex AF patients. Methods: From the ESC-EHRA EORP-AF General Long-Term Registry, we analysed clinically complex AF patients, defined as the presence of frailty, multimorbidity and/or polypharmacy. A K-medoids cluster analysis was performed to identify different groups of clinical complexity. The impact of an ABC-adherent approach on major outcomes was analysed through Cox-regression analyses and delay of event (DoE) analyses. Results: Among 9966 AF patients included, 8289 (83.1%) were clinically complex. Adherence to the ABC pathway in the clinically complex group reduced the risk of all-cause death (adjusted HR [aHR]: 0.72, 95%CI 0.58–0.91), major adverse cardiovascular events (MACEs; aHR: 0.68, 95%CI 0.52–0.87) and composite outcome (aHR: 0.70, 95%CI: 0.58–0.85). Adherence to the ABC pathway was associated with a significant reduction in the risk of death (aHR: 0.74, 95%CI 0.56–0.98) and composite outcome (aHR: 0.76, 95%CI 0.60–0.96) also in the high-complexity cluster; similar trends were observed for MACEs. In DoE analyses, an ABC-adherent approach resulted in significant gains in event-free survival for all the outcomes investigated in clinically complex patients. Based on absolute risk reduction at 1 year of follow-up, the number needed to treat for ABC pathway adherence was 24 for all-cause death, 31 for MACEs and 20 for the composite outcome. Conclusions: An ABC-adherent approach reduces the risk of major outcomes in clinically complex AF patients. Ensuring adherence to the ABC pathway is essential to improve clinical outcomes among clinically complex AF patients