First report of Trypanosoma cruzi infection in naturally infected dogs from southern Bahia, Brazil Primeiro relato de infecção natural por Trypanosoma cruzi em cães do sul da Bahia, Brasil

In order to verify the Trypanosoma cruzi infection in domestic domiciled dogs in a rural endemic area from the south region of the State of Bahia, Polymerase Chain Reaction (PCR) were performed using S35 and S36 primers in 272 dogs living in the district of Vila Operaria, in the municipality of Buerarema. All animals were clinically evaluated; 2.5 mL of blood were collected through venipuncture for the performance of molecular tests. None of these animals showed clinical signs of the illness and only two were identified with the DNA parasite. This result is the first report of natural infection by T. cruzi in domestic dogs in southern Bahia.<br>Com o objetivo de verificar a infec&#231;&#227;o por Trypanosoma cruzi em c&#227;es dom&#233;sticos domiciliados em &#225;rea rural e end&#234;mica do sul da Bahia, foi realizada a Rea&#231;&#227;o em Cadeia da Polimerase (PCR), utilizando-se os iniciadores S35 e S36 em 272 c&#227;es domiciliados no distrito da Vila Oper&#225;ria, cidade de Buerarema. Todos os animais foram avaliados clinicamente e, posteriormente, foram coletados 2,5 mL de sangue por pun&#231;&#227;o venosa para realiza&#231;&#227;o do diagn&#243;stico molecular. Nenhum dos animais apresentou manifesta&#231;&#227;o cl&#237;nica da doen&#231;a e, em apenas dois foram identificados DNA do parasito. Esse resultado &#233; o primeiro relato de infec&#231;&#227;o natural por T. cruzi em c&#227;es dom&#233;sticos no sul baiano

First report of Trypanosoma cruziinfection in naturally infected dogs from southern Bahia, Brazil

In order to verify the Trypanosoma cruzi infection in domestic domiciled dogs in a rural endemic area from the south region of the State of Bahia, Polymerase Chain Reaction (PCR) were performed using S35 and S36 primers in 272 dogs living in the district of Vila Operaria, in the municipality of Buerarema. All animals were clinically evaluated; 2.5 mL of blood were collected through venipuncture for the performance of molecular tests. None of these animals showed clinical signs of the illness and only two were identified with the DNA parasite. This result is the first report of natural infection by T. cruzi in domestic dogs in southern Bahia

Epidemiology of canine leishmaniasis in southern Bahia, Brazil Nilo

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2015-05-28T17:07:28Z No. of bitstreams: 1 Leça Junior NF Epidemiology....pdf: 313446 bytes, checksum: 2e1aa7a47df4454b56ddcf85b2ed3f6f (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2015-05-28T17:21:24Z (GMT) No. of bitstreams: 1 Leça Junior NF Epidemiology....pdf: 313446 bytes, checksum: 2e1aa7a47df4454b56ddcf85b2ed3f6f (MD5)Made available in DSpace on 2015-05-28T17:21:24Z (GMT). No. of bitstreams: 1 Leça Junior NF Epidemiology....pdf: 313446 bytes, checksum: 2e1aa7a47df4454b56ddcf85b2ed3f6f (MD5) Previous issue date: 2015Ciência Animal. Araguaína, TO, BrasilUniversidade Estadual de Santa Cruz. UESC. Pós-Graduação em Ciência Animal. Ilhéus, BA, BrasilVeterinária. Ilhéus, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Estadual de Santa Cruz. UESC. Pós—Graduação em Genética e Biologia Molecular. Ilhéus, BA, BrasilUniversidade Estadual de Santa Cruz. UESC. Hospital Veterinário. Departamento de Ciências Agrárias e Ambientais. Ilhéus, BA, BrasilUniversidade Estadual de Santa Cruz. UESC. Hospital Veterinário. Departamento de Ciências Agrárias e Ambientais. Ilhéus, BA, BrasilUniversidade Estadual de Santa Cruz. UESC. Hospital Veterinário. Departamento de Ciências Agrárias e Ambientais. Ilhéus, BA, BrasilUniversidade Estadual de Santa Cruz. UESC. Hospital Veterinário. Departamento de Ciências Agrárias e Ambientais. Ilhéus, BA, BrasiltLeishmaniosis is a zoonosis caused by protozoa of the genus Leishmania. American cutaneous leishman-iosis (ACL) is mainly caused by the species L. amazonensis and L. braziliensis, and American visceralleishmaniosis (AVL) is caused by L. infantum chagasi. In addition to their proven roles as reservoirs ofAVL, dogs are also suspected by researchers to be reservoirs of ACL due to reports of this infection indomestic environments and of infected dogs in endemic areas. The aim of this study was to detect Leish-mania sp. infection in dogs from Vila Operária, Buerarema, Bahia, using parasitological tests, indirectimmunofluorescent assay (IFA) and polymerase chain reaction (PCR). Furthermore, this study also aimedto identify risk factors associated with illness in dogs in this locality by conducting an epidemiologicalsurvey. For this purpose, 292 dogs were clinically evaluated for the presence of skin lesions, and thedogs that showed these changes were submitted to scarification injury to enable preparation of slidesfor microscopic study of amastigotes. Subsequently, the dogs underwent blood sampling for serological(IFA) and molecular (PCR) tests. Additionally, the owners of the dogs answered an epidemiological ques-tionnaire to facilitate the identification of risk factors for exposure of dogs to pathogens of ACL. Of the 292dogs studied, 13 (4.5%) had lesions suggestive of ACL, but with a negative parasitological examinationand 147 (50.3%) were seropositive according to the IFA. Of the 273 dogs studied using PCR test, 10 (3.66%)were positive for L. braziliensis, and all samples were negative for L. infantum chagasi. Wastelands in theperidomicile and the presence of light in the household were risk factors associated with ACL. The resultsshow that Vila Operária has asymptomatic dogs with ACL and that the detection sensitivity of the IFAwas higher than that of PCR for the infected dogs