241 research outputs found
Was fĂĽr ein Perfekt gibt es im modernen Polnisch? Bemerkungen zum Artikel "Gibt es ein Perfekt im modernen Polnisch?" von H. Weydt und A. Kazimierczak (Linguistik online 4, 3/99)
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A bacteriophage encodes its own CRISPR/Cas adaptive response to evade host innate immunity
Lançamento de produto na indústria de móveis para escritórios de pequeno porte : um estudo de caso
Orientador : Freddy KesselringMonografia (especialização) - Universidade Federal do Paraná, Curso de Especialização em MarketingInclui referência
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Genes Contributing to Staphylococcus aureus Fitness in Abscess- and Infection-Related Ecologies
ABSTRACT Staphylococcus aureus is a leading cause of both community- and hospital-acquired infections that are increasingly antibiotic resistant. The emergence of S. aureus resistance to even last-line antibiotics heightens the need for the development of new drugs with novel targets. We generated a highly saturated transposon insertion mutant library in the genome of S. aureus and used Tn-seq analysis to probe the entire genome, with unprecedented resolution and sensitivity, for genes of importance in infection. We further identified genes contributing to fitness in various infected compartments (blood and ocular fluids) and compared them to genes required for growth in rich medium. This resulted in the identification of 426 genes that were important for S. aureus fitness during growth in infection models, including 71 genes that could be considered essential for survival specifically during infection. These findings highlight novel as well as previously known genes encoding virulence traits and metabolic pathways important for S. aureus proliferation at sites of infection, which may represent new therapeutic targets
Hepatitis Delta Virus RNA Replication
Hepatitis delta virus (HDV) is a distant relative of plant viroids in the animal world. Similar to plant viroids, HDV replicates its circular RNA genome using a double rolling-circle mechanism. Nevertheless, the production of hepatitis delta antigen (HDAg), which is indispensible for HDV replication, is a unique feature distinct from plant viroids, which do not encode any protein. Here the HDV RNA replication cycle is reviewed, with emphasis on the function of HDAg in modulating RNA replication and the nature of the enzyme involved
NUTRITIONAL ANALYSIS AND AMINO ACID PROFILE OF SHIITAKE MUSHROOM (Lentinus edodes, Agaricaceae)
O Lentinus edodes - cogumelo comestĂvel pertencente Ă famĂlia Agaricaceae e popularmente conhecido como shitake – Ă© apreciado há sĂ©culos na culinária e medicina oriental devido ao seu sabor agradável e alto valor nutritivo. Visando destacar os benefĂcios do L. edodes, o presente trabalho teve como objetivo a realização da análise nutricional desse alimento. A qualidade do L. edodes como fonte protĂ©ica tambĂ©m foi avaliada e comparada com a carne bovina, o feijĂŁo e a soja, considerando que estes alimentos, assim como os cogumelos, sĂŁo ricos em proteĂnas. Para avaliação do valor nutricional protĂ©ico do L. edodes, foram calculados os escores quĂmicos de seus aminoácidos essenciais. Para fins de comparação, tambĂ©m foram utilizados os valores de digestibilidade protĂ©ica para o L. edodes, carne bovina, feijĂŁo e soja. O L. edodes analisado apresentou elevado teor protĂ©ico (17,14%), baixo teor de gorduras (1,23%) e baixa digestibilidade comparado Ă carne bovina. Os valores dos escores quĂmicos para o L. edodes apresentaram-se todos maiores do que 1,0, indicando que suas proteĂnas possuem alto valor biolĂłgico, assim como a carne bovina. Os resultados obtidos mostram que o L. edodes apresenta proteĂnas de maior valor nutricional do que o feijĂŁo e a soja, sendo inferior Ă carne bovina neste quesito.   Palavras-chave: shitake; Lentinus edodes; valor nutricional, aminoácido
A core microbiome associated with the peritoneal tumors of pseudomyxoma peritonei
Pseudomyxoma peritonei (PMP) is a malignancy characterized by dissemination of mucus-secreting cells throughout the peritoneum. This disease is associated with significant morbidity and mortality and despite effective treatment options for early-stage disease, patients with PMP often relapse. Thus, there is a need for additional treatment options to reduce relapse rate and increase long-term survival. A previous study identified the presence of both typed and non-culturable bacteria associated with PMP tissue and determined that increased bacterial density was associated with more severe disease. These findings highlighted the possible role for bacteria in PMP disease. To more clearly define the bacterial communities associated with PMP disease, we employed a sequenced-based analysis to profile the bacterial populations found in PMP tumor and mucin tissue in 11 patients. Sequencing data were confirmed by in situ hybridization at multiple taxonomic depths and by culturing. A pilot clinical study was initiated to determine whether the addition of antibiotic therapy affected PMP patient outcome. We determined that the types of bacteria present are highly conserved in all PMP patients; the dominant phyla are the Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes. A core set of taxon-specific sequences were found in all 11 patients; many of these sequences were classified into taxonomic groups that also contain known human pathogens. In situ hybridization directly confirmed the presence of bacteria in PMP at multiple taxonomic depths and supported our sequence-based analysis. Furthermore, culturing of PMP tissue samples allowed us to isolate 11 different bacterial strains from eight independent patients, and in vitro analysis of subset of these isolates suggests that at least some of these strains may interact with the PMP-associated mucin MUC2. Finally, we provide evidence suggesting that targeting these bacteria with antibiotic treatment may increase the survival of PMP patients. Using 16S amplicon-based sequencing, direct in situ hybridization analysis and culturing methods, we have identified numerous bacterial taxa that are consistently present in all PMP patients tested. Combined with data from a pilot clinical study, these data support the hypothesis that adding antimicrobials to the standard PMP treatment could improve PMP patient survival.https://doi.org/10.1186/1750-1172-8-10
Coupled Folding and Specific Binding: Fishing for Amphiphilicity
Proteins are uniquely capable of identifying targets with unparalleled selectivity, but, in addition to the precision of the binding phenomenon, nature has the ability to find its targets exceptionally quickly. Transcription factors for instance can bind to a specific sequence of nucleic acids from a soup of similar, but not identical DNA strands, on a timescale of seconds. This is only possible with the enhanced kinetics provided for by a natively disordered structure, where protein folding and binding are cooperative processes. The secondary structures of many proteins are disordered under physiological conditions. Subsequently, the disordered structures fold into ordered structures only when they bind to their specific targets. Induced folding of the protein has two key biological advantages. First, flexible unstructured domains can result in an intrinsic plasticity that allows them to accommodate targets of various size and shape. And, second, the dynamics of this folding process can result in enhanced binding kinetics. Several groups have hypothesized the acceleration of binding kinetics is due to induced folding where a “fly-casting” effect has been shown to break the diffusion-limited rate of binding. This review describes experimental results in rationally designed peptide systems where the folding is coupled to amphiphilicity and biomolecular activity
ANTI AGING CLOVE ESSENTIAL OIL MICROPARTICLES
Radicais livres sĂŁo capazes de aumentar o fenĂ´meno natural de dano Ă pele, estresse oxidativo e peroxidação de ácidos graxos da bicamada lipĂdica. Para impedir este processo, a pele possui o seu prĂłprio mecanismo de defesa. No entanto esta habilidade de proteção natural diminui com o avanço da idade. As propriedades antioxidantes do Ăłleo essencial de cravo-da-Ăndia (Caryophyllus aromaticus Teysm) encapsulado em micropartĂculas de alginato de sĂłdio foram utilizadas no desenvolvimento de formulações   dermatolĂłgicas para cuidados com a pele
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