159 research outputs found
Tools for Assessing Climate Impacts on Fish and Wildlife
Climate change is already affecting many fish and wildlife populations. Managing these populations requires an understanding of the nature, magnitude, and distribution of current and future climate impacts. Scientists and managers have at their disposal a wide array of models for projecting climate impacts that can be used to build such an understanding. Here, we provide a broad overview of the types of models available for forecasting the effects of climate change on key processes that affect fish and wildlife habitat (hydrology, fire, and vegetation), as well as on individual species distributions and populations. We present a framework for how climate-impacts modeling can be used to address management concerns, providing examples of model-based assessments of climate impacts on salmon populations in the Pacific Northwest, fire regimes in the boreal region of Canada, prairies and savannas in the Willamette Valley-Puget Sound Trough-Georgia Basin ecoregion, and marten Martes americana populations in the northeastern United States and southeastern Canada. We also highlight some key limitations of these models and discuss how such limitations should be managed. We conclude with a general discussion of how these models can be integrated into fish and wildlife management
Analysis of the Sam50 translocase of excavate organisms supports evolution of divergent organelles from a common endosymbiotic event
As free-living organisms the ancestors of mitochondria and plastids encoded complete genomes, proteomes and metabolomes. As these symbionts became organelles all these aspects were reduced – genomes have degenerated with the host nucleus now encoding the most of the remaining endosymbiont proteome, while the metabolic processes of the symbiont have been streamlined to the functions of the emerging organelle. By contrast, the topology of the endosymbiont membrane has been preserved, necessitating the development of complex pathways for membrane insertion and translocation. In this study, we examine the characteristics of the endosymbiont-derived β-barrel insertase Sam501 in the excavate super-group. A candidate is further characterized in Trichomonas vaginalis, an unusual eukaryote possessing degenerate hydrogen-producing mitochondria called hydrogenosomes. This information supports a mitochondriate eukaryotic common ancestor with a similarly evolved β-barrel insertase, which has continued to be conserved in degenerate mitochondria
Heterologous Expression of a Membrane-Spanning Auxin Importer: Implications for Functional Analyses of Auxin Transporters
Biochemical studies of plant auxin transporters
in vivo are made difficult by the presence of
multiple auxin transporters and auxin-interacting
proteins. Furthermore, the expression level of most
such transporters in plants is likely to be too low
for purification and downstream functional
analysis. Heterologous expression systems should
address both of these issues. We have examined a
number of such systems for their efficiency in
expressing AUX1 from Arabidopsis
thaliana. We find that a eukaryotic system
based upon infection of insect cells with
recombinant baculovirus provides a high level,
easily scalable expression system capable of
delivering a functional assay for AUX1.
Furthermore, a transient transfection system in
mammalian cells enables localization of AUX1 and
AUX1-mediated transport of auxin to be
investigated. In contrast, we were unable to
utilise P. pastoris or L. lactis expression systems to reliably express AUX1
Analysis of the Sam50 translocase of excavate organisms supports evolution of divergent organelles from a common endosymbiotic event
As free-living organisms the ancestors of mitochondria and plastids encoded complete genomes, proteomes and metabolomes. As these symbionts became organelles all these aspects were reduced – genomes have degenerated with the host nucleus now encoding the most of the remaining endosymbiont proteome, while the metabolic processes of the symbiont have been streamlined to the functions of the emerging organelle. By contrast, the topology of the endosymbiont membrane has been preserved, necessitating the development of complex pathways for membrane insertion and translocation. In this study, we examine the characteristics of the endosymbiont-derived β-barrel insertase Sam501 in the excavate super-group. A candidate is further characterized in Trichomonas vaginalis, an unusual eukaryote possessing degenerate hydrogen-producing mitochondria called hydrogenosomes. This information supports a mitochondriate eukaryotic common ancestor with a similarly evolved β-barrel insertase, which has continued to be conserved in degenerate mitochondria
Implications for oxidative stress and astrocytes following 26S proteasomal depletion in mouse forebrain neurones
Neurodegenerative diseases are characterized by progressive degeneration of selective neurones in the nervous system, but the underlying mechanisms involved in neuroprotection and neurodegeneration remain unclear. Dysfunction of the ubiquitin proteasome system is one of the proposed hypotheses for the cause and progression of neuronal loss. We have performed quantitative two-dimensional fluorescence difference in-gel electrophoresis combined with peptide mass fingerprinting to reveal proteome changes associated with neurodegeneration following 26S proteasomal depletion in mouse forebrain neurones. Differentially expressed proteins were validated by Western blotting, biochemical assays and immunohistochemistry. Of significance was increased expression of the antioxidant enzyme peroxiredoxin 6 (PRDX6) in astrocytes, associated with oxidative stress. Interestingly, PRDX6 is a bifunctional enzyme with antioxidant peroxidase and phospholipase A2 (PLA2) activities. The PLA2 activity of PRDX6 was also increased following 26S proteasomal depletion and may be involved in neuroprotective or neurodegenerative mechanisms. This is the first in vivo report of oxidative stress caused directly by neuronal proteasome dysfunction in the mammalian brain. The results contribute to understanding neuronal–glial interactions in disease pathogenesis, provide an in vivo link between prominent disease hypotheses and importantly, are of relevance to a heterogeneous spectrum of neurodegenerative diseases
Immune-Instructive Polymers Control Macrophage Phenotype and Modulate the Foreign Body Response In Vivo
© 2020 The Author(s) Implantation of medical devices can result in inflammation. A large library of polymers is screened, and a selection found to promote macrophage differentiation towards pro- or anti-inflammatory phenotypes. The bioinstructive properties of these materials are validated within a rodent model. By identifying novel materials with immune-instructive properties, the relationship between material-immune cell interactions could be investigated, and this offers exciting possibilities to design novel bioinstructive materials that can be used for numerous clinical applications including medical implants
Exploring Human/Animal Intersections: Converging Lines of Evidence in Comparative Models of Aging
At a symposium convened on March 8, 2007 by the Institute on Aging at the University of Pennsylvania, researchers from the University’s Schools of Medicine and Veterinary Medicine explored the convergence of aging research emerging from the two schools. Studies in human patients, animal models, and companion animals have revealed different but complementary aspects of the aging process, ranging from fundamental biologic aspects of aging to the treatment of age-related diseases, both experimentally and in clinical practice. Participants concluded that neither animal nor human research alone will provide answers to most questions about the aging process. Instead, an optimal translational research model supports a bidirectional flow of information from animal models to clinical research
Ultra-fast yttrium hydride chemistry at high pressures via non-equilibrium states induced by x-ray free electron laser
Controlling the formation and stoichiometric content of desired phases of
materials has become a central interest for the study of a variety of fields,
notably high temperature superconductivity under extreme pressures. The further
possibility of accessing metastable states by initiating reactions by x-ray
triggered mechanisms over ultra-short timescales is enabled with the
development of x-ray free electron lasers (XFEL). Utilizing the exceptionally
high brilliance x-ray pulses from the EuXFEL, we report the synthesis of a
previously unobserved yttrium hydride under high pressure, along with
non-stoichiometric changes in hydrogen content as probed at a repetition rate
of 4.5\,MHz using time-resolved x-ray diffraction. Exploiting non-equilibrium
pathways we synthesize and characterize a hydride with yttrium cations in an
\textit{A}15 structure type at 125\,GPa, predicted using crystal structure
searches, with a hydrogen content between 4.0--5.75 hydrogens per cation, that
is enthalpically metastable on the convex hull. We demonstrate a tailored
approach to changing hydrogen content using changes in x-ray fluence that is
not accessible using conventional synthesis methods, and reveals a new paradigm
in metastable chemical physics
Microparticles Decorated with Cell‐Instructive Surface Chemistries Actively Promote Wound Healing
Wound healing is a complex biological process involving close crosstalk between various cell types. Dysregulation in any of these processes, such as in diabetic wounds, results in chronic non-healing wounds. Fibroblasts are a critical cell type involved in the formation of granulation tissue, essential for effective wound healing. We screened 315 different polymer surfaces to identify candidates which actively drove fibroblasts towards either pro- or anti-proliferative functional phenotypes. Fibroblast-instructive chemistries were identified, which we synthesized into surfactants to fabricate easy to administer microparticles for direct application to diabetic wounds. The pro-proliferative microfluidic derived particles were able to successfully promote neovascularisation, granulation tissue formation and wound closure after a single application to the wound bed. These active novel 3D bio-instructive microparticles show great potential as a route to reducing the burden of chronic wounds
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