Lipidomics for robust high performance process development

As the biopharmaceutical industry reduces the risk of potential contaminations by adventitious agents and increases process yields, high performance cell culture processes have been developed that rely on animal-free peptide-free, protein-free chemically defined and lipid-free media. These processes rely on cell lines that have been adapted to these lipid-free production conditions and have developed very effective lipid production capacities. These lipid-production capacities result in new challenges in the harvest and purification steps such as filterability, ability to clean resins and resin reuse. This oral presentation will show case how lipidomics can provide insights and opportunities to control the interactions between high performance bioreactor production processes, harvest conditions and purification. Results obtained across large scale production processes of three different monoclonal antibodies will be displayed. The importance of controlling lipid biosynthesis and the presence of lipids in the cell culture fluid prior to affinity capture chromatography will be discussed. Three different control strategies will be showcased and their pros and cons in terms of process yields, robustness of the harvest and impact on the purification process post-harvest explaine

Influence of pre-Andean history over Andean foreland deformation: structural styles in the Malargüe fold-and-thrust belt at 35ºS, Andes of Argentina

We present the first complete balanced cross-section of the Argentinean Andes at 35ºS. Based on an extensive field survey and limited sub-surface information, we constructed a structural model in which both the tectonic inversion of Mesozoic normal faults and the formation of Cenozoic Andean thrusts play a role in the deformation. We obtained a shortening of 26.2 km, equivalent to 22% of the initial length. This value is lower than previous estimates obtained from partial cross-sections using non-inversion structural models. Comparison of our results with a geophysical model of the crust indicates that: (i) crustal thickness was not constant across the orogen before Andean shortening, but a thick (~45 km) crustal block was interpreted? in the west as a remnant of a Late Paleozoic orogeny, and a thinner sector (~32 km) was located in the east as a result of Mesozoic stretching; and (ii) the structural model presented in this work is more consistent with the regional shortening and crustal thickness trends than models which do not take into account tectonic inversion.Fil: Mescua, Jose Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; ArgentinaFil: Giambiagi, Laura Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; ArgentinaFil: Tassara Oddo, Andres Humberto. Universidad de Concepción; ChileFil: Gimenez, Mario Ernesto. Universidad Nacional de San Juan. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto Geofísico Sismológico Volponi; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ramos, Victor Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Estudios Andinos "Don Pablo Groeber". Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Estudios Andinos "Don Pablo Groeber"; Argentin

Towards Conceptual Modelling Interoperability in a Web Tool for Ontology Engineering

The definition of suitable visual paradigms for ontology modelling is still an open issue. Despite obvious differences between the expressiveness of conceptual modelling (CM) languages and ontologies, many proposed tools have been based on UML, EER and ORM. Additionally, all of these tools support only one CM as visual language, reducing even more their modelling capabilities. In previous works, we have presented crowd as a Web architecture for graphical ontology designing in UML and logical reasoning to verify the relevant properties of these models. The aim of this tool is to extend the reasoning capabilities on top of visual representations as much as possible. In this paper, we present an extended crowd architecture and a new prototype focusing on an ontology-driven metamodel to enable different CMs visual languages for ontology modelling. Thus facilitating inter-model assertions across models represented in different languages, converting between modelling languages and reasoning on them. Finally, we detail the new architecture and demonstrate the usage of the prototype with simple examples

Energy reserves mobilization: Strategies of three decapod species

In food deprivation assays, several different responses have been observed in crustaceans. However, studying energy reserves utilization among more than one species during the same starvation period has not yet been performed, particularly to discern whether the responses are due to intrinsic and/or environmental factors. We hypothesize that decapod species with similar feeding habits have the same strategies in the use of energetic reserves during starvation, even though they inhabit different environments. The aim of this study was to compare the energy reserves mobilization of three decapods species (Cherax quadricarinatus, Palaemon argentinus and Munida gregaria) with similar feeding habits, exposed to similar food deprivation conditions. The crayfish, shrimp and squat-lobster were experimentally kept at continuous feeding or continuous starvation throughout 15 days. Every 3rd day, the midgut gland index (MGI), and the glycogen, lipid and protein contents were measured in the midgut gland (MG) and pleon muscle. Palaemon argentinus mobilized more reserves during starvation, followed by C. quadricarinatus, and the last M. gregaria. The starved shrimps presented low MGI, whereas MG showed a reduction in glycogen (from day 6 to 15), lipid (from day 3 to 15), and protein levels (at day 9 and 15) while in their muscle, lipid reserves decreased at days 3 and 6. In C. quadricarinatus, the most affected parameters in the MG were MGI, glycogen (from day 6 to 15), and lipids (at day 12 and 15). In the MG of M. gregaria only the glycogen was reduced during fasting from 3 to 15 days. Even though the three studied species have similar feeding habitats, we found that their energetic profile utilization is different and it could be explained by the habitat, life span, temperature, organ/tissue, and metabolism of the species. Our results may be useful to understand the several different responses of crustaceans during starvation.Fil: Sacristán, Hernán Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Rodriguez, Yamila Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Pereira, Nair de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Lopez, Laura Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Lovrich, Gustavo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Fernandez Gimenez, Analia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentin

Interpretation of cyclic voltammetry measurements of thin semiconductor films for solar fuel applications

A simple model is proposed that allows interpretation of the cyclic voltammetry diagrams obtained experimentally for photoactive semiconductors with surface states or catalysts used for fuel production from sunlight. When the system is limited by charge transfer from the traps/catalyst layer and by detrapping, it is shown that only one capacitive peak is observable and is not recoverable in the return voltage scan. If the system is limited only by charge transfer and not by detrapping, two symmetric capacitive peaks can be observed in the cathodic and anodic directions. The model appears as a useful tool for the swift analysis of the electronic processes that limit fuel production.The research leading to these results is supported by Universitat Jaume I Project P1·1B2011-50

Indicadores e índices de calidad en suelos rojos bajo sistemas naturales y cultivados

Los objetivos de este trabajo fueron: determinar indicadores de calidad de suelo, desarrollar índices funcionales y aplicar índices utilizados en la bibliografía, a fin de evaluar su sensibilidad para detectar los cambios producidos por el desmonte y posterior uso agrícola en Oxisoles del departamento de Oberá (Misiones). Se empleó un diseño completamente al azar, con tres tratamientos: Selva (S), cultivo de maíz con labranza convencional (Zea mays L.) (M) y cultivo de Té (Camellia sinensis L.) (T). Se muestrearon 12 lotes por tratamiento a 0-0,10; 0,10-0,20 y 0,20-0,30 m. Las variables evaluadas fueron: respiración (RES), densidad aparente (Da), textura, pH, materia orgánica total (MO) y particulada (MOP), nitrógeno total (Nt) y potencialmente mineralizable (NPM). Se determinaron los índices: MOP/MO, NPM/Nt, NPM/MOP, MO/Li+Arc, MOP/RES y las relaciones de estratificación del carbono orgánico del suelo (COSr1, COSr2) y del potencial de mineralización del nitrógeno (NPMr1, NPMr2). Los resultados se analizaron mediante ANOVA, Test LSD (p < 0,05), y correlación de Pearson. Los indicadores: Da, RES, MO, Nt, y NPM y los índices NPM/Nt, MO/Li+Arc, NPM/MOP, IRC, IMC, COSr1, COSr2, permitieron diferenciar suelos bajo sistemas naturales de cultivados. La Da, la RES y el índice COSr2 resultaron más sensibles, diferenciando también sistemas agrícolas: cultivo perenne de anual, indicando a este último como el más degradante. Si bien resultan necesarias más investigaciones, estos resultados indicarían que suelos de alta calidad, presentarían un índice de estratificación COSr1 >2 y un COSr2 >1,5 por lo que valores menores indicarían pérdida de calidad. El índice MOP/RES desarrollado detectó diferencias en la calidad de MOP y su ciclado, resultando los suelos vírgenes y bajo cultivo perenne, sin remoción de suelo (T), con un ciclado más lento que los suelos bajo cultivo anual (M).The objectives of this study were to determine soil quality (SQ) indicators, develop proper soil quality indices and apply the indices from the literature so as to assess their sensitivity in detecting the SQ changes caused by deforestation and agricultural use in Oxisols at Oberá (Misiones), where rainforests have been removed and replaced with annual and perennial crops. The experimental design was completely randomized with three treatments: tropical forest (S), corn crop under conventional tillage (Zea Mays L.) (M) and tea crop (Camellia sinensis L.) (T). Twelve plots were sampled from each treatment at 0-0,10; 0,10-0,20 and 0,20-0,30 m depths. The following variables were evaluated on the soil samples: respiration (RES), bulk density (Da), texture, pH, organic matter (MO), particulate organic matter (MOP), total nitrogen (Nt), and potentially mineralizable nitrogen (NPM). The following indices were determined: MOP/MO, NPM/Nt, NPM/MOP, MO/Li+Arc, MOP/RES, and the stratification ratios of soil organic carbon (COSr1, COSr2) and of nitrogen mineralization potential (NPMr1, NPMr2). All data were analyzed through ANOVA, means were separated by the LSD Test (p < 0,05), and a Pearson correlation was performed. Soil indicators Da, RES, MO, Nt and NPM, and indices NPM/Nt, MO/Li+Arc, NPM/MOP, IRC, IMC, COSr1 and COSr2 revealed statistical differences between soils under natural systems and cultivated soils. Indicators Da and RES, and index COSr2 turned out to be more sensitive; they showed differences between perennial and annual cropping systems, the latter causing higher soil degradation. Even though further research is needed, the results obtained from this study would indicate that high quality soils have a stratification ratio COSr1 >2 and COSr2 >1,5; lower values would therefore suggest loss of SQ. The elaborated ratio MOP/RES showed differences in MOP quality and its cycling, indicating that in virgin soils and perennial cropping systems without removal of soil (T), the cycling is slower than in soils under annual crops (M)

Infectious bursal disease virus uptake involves macropinocytosis and trafficking to early endosomes in a Rab5-dependent manner

ARTÍCULO PUBLICADO EN REVISTA EXTERNA. Infectious bursal disease virus (IBDV) internalization is sparsely known in terms of molecular components of the pathway involved. To describe the cell biological features of IBDV endocytosis, we employed perturbants of endocytic pathways such as pharmacological inhibitors and overexpression of dominant-negative mutants. Internalization analysis was performed quantifying infected cells by immunofluorescence and Western blot detection of the viral protein VP3 at 12 h post-infection reinforced by the analysis of the capsid protein VP2 localization after virus uptake at 1 h post-infection. We compared IBDV infection to the internalization of well-established ligands with defined endocytic pathways: transferrin, cholera-toxin subunit B and dextran. To describe virus endocytosis at the morphological level, we performed ultrastructural studies of viral internalization kinetics in control and actin dynamics-blocked cells. Our results indicate that IBDV endocytic internalization was clathrin- and dynamin-independent, and that IBDV uses macropinocytosis as the primary entry mechanism. After uptake, virus traffics to early endosomes and requires exposure to the low endocytic pH as well as a functional endocytic pathway to complete its replication cycle. Moreover, our results indicate that the GTPase Rab5 is crucial for IBDV entry supporting the participation of the early endosomal pathway in IBDV internalization and infection of susceptible cells. Sitio de la revista: https://onlinelibrary.wiley.com/doi/full/10.1111/cmi.1241

Organic photoelectrochemical cells with quantitative photocarrier conversion

Efficient solar-to-fuel conversion could be a cost-effective way to power the planet using sunlight. Herein, we demonstrate that Organic Photoelectrochemical Cells (OPECs) constitute a versatile platform for the efficient production of solar fuels. We show that the photogenerated carriers at the organic active layer can be quantitatively extracted to drive photoelectrochemical reactions at the interface with a liquid solution. Indeed, an unprecedented photocurrent of 4 mA cm−2 is extracted for an OPEC device, comparable to that of a solid-state device with similar optical properties. Through the careful choice of the selective contact and the redox couple in the liquid medium, we can tune the energetics of the system and activate either oxidative or reductive chemistry. The design rules to drive the desired electrochemical reaction are provided based on a comprehensive study of the energetic aspects of OPEC configuration. Finally, we demonstrate that OPEC devices effectively produce hydrogen in acetonitrile when a cobaloxime based homogeneous catalyst is present in the solution, and HCl is used a source of protons

New Views on Carrier Diffusion and Recombination by Combining Small Perturbation Techniques: Application to BiVO4 Photoelectrodes

Impedance spectroscopy (IS), intensity-modulated photocurrent spectroscopy (IMPS), and intensity-modulated photovoltage spectroscopy (IMVS) are well-established powerful modulated techniques to characterize optoelectronic devices. Their combined use has proven to provide an understanding of the behavior and performance of these systems, far beyond the output obtained from their independent analysis. However, this combination is shown to be challenging when applied to complex systems. Herein, IS, IMPS, and IMVS are cooperatively used, for the first time, to study the distributed photogeneration, diffusion, and recombination processes in a photoanode of zircon-doped bismuth vanadate. The use of this methodology reveals that the carriers that determine the response of the device are the electrons when the device is illuminated from the hole-collector side (electrolyte) and the holes when the illumination reaches the device from the electron-collector side. Detailed quantitative information is obtained for each carrier, including recombination lifetime, diffusion coefficient and collectrion and separation efficiencies, identifying the latter as the main limitation of this device. This methodology is a powerful tool that can be used for the characterization and understanding of the operating processes of other photoconversion devices.Funding for open access charge: CRUE-Universitat Jaume

Facile Surfactant-Assisted Synthesis of BiVO4 Nanoparticulate Films for Solar Water Splitting

Bismuth vanadate (BiVO4), which has attractive applicability as a photoactive material, presents applications that range from catalysis to water treatment upon visible light irradiation. In this study, we develop a simple synthesis of < 200 nm monoclinic BiVO4 nanoparticles, which were further deposited on transparent conductive substrates by spin coating and calcination, obtaining nanostructured films. The obtained nanostructured BiVO4 photoanodes were tested for water oxidation, leading to promising photocurrents exhibiting competitive onset potentials (~0.3 V vs. RHE). These nanoparticulate BiVO4 photoanodes represent a novel class of highly potential materials for the design of efficient photoelectrochemical devices