Assembly of Bleomycin Saccharide-Decorated Spherical Nucleic Acids

Glyco-decorated spherical nucleic acids (SNAs) may be attractive delivery vehicles, emphasizing the sugar-specific effect on the outer sphere of the construct and at the same time hiding unfavorable distribution properties of the loaded oligonucleotides. As examples of such nanoparticles, tripodal sugar constituents of bleomycin were synthesized and conjugated with a fluorescence-labeled antisense oligonucleotide (AONARV7). Successive copper(I)-catalyzed azide-alkyne and strain-promoted alkyne-nitrone cycloadditions (SPANC) were utilized for the synthesis. Then, the glyco-AONARV7 conjugates were hybridized with complementary strands of a C60-based molecular spherical nucleic acid (i.e., a hybridization-mediated carrier). The formation and stability of these assembled glyco-decorated SNAs were evaluated by polyacrylamide gel electrophoresis (PAGE), UV melting profile analysis, and time-resolved fluorescence spectroscopy. Association constants were extracted from time-resolved fluorescence data. Preliminary cellular uptake experiments of the glyco-AONARV7 conjugates (120 nM solutions) and of the corresponding glyco-decorated SNAs (10 nM solutions) with human prostate cancer cells (PC3) showed an efficient uptake in each case. A marked variation in intracellular distribution was observed.</p

Kuhinaa kulisseissa : Kun suomalainen mies myy potenssiaan

Perustuu Helsingin yliopiston sosiologian laitokselle tehtyyn pro gradu -tutkielmaa

High-Entropy Carbides:Processing And Characterization

High entropy carbides (HEC) are multi-metal carbides involving at least five types of metal atoms at near-equal concentrations. They exhibit crystalline periodicity and a precise carbon sublattice but display disorder in terms of metal cation packing, therefore, they also have unconventional mechanical and physical properties. Up till now, the high entropy carbide compositions presented in literature have been rich in critical raw materials (CRM), such as hafnium (Hf). We explore the feasibility of fabricating sustainable high-entropy carbide compositions, free of CRMs, using conventional ceramic processing techniques, such as mechanical milling and thermal treatments. Five compositions of multi-metal carbides were chosen based on their entropy forming ability from literature. Metal powders and carbon were mixed in stoichiometric proportions using ball milling and sintered at high temperatures (2000 ºC). X-ray and electron microscopy techniques were used to characterize the materials and verify the formation of single-phase multi-metal carbides and chemical homogeneity at micrometer length scales. In addition, the mechanical properties of a selected composition was studied using nanoindentation.</p

Assembly of Bleomycin Saccharide-Decorated Spherical Nucleic Acids

Glyco-decorated spherical nucleic acids (SNAs) may be attractive delivery vehicles, emphasizing the sugar-specific effect on the outer sphere of the construct and at the same time hiding unfavorable distribution properties of the loaded oligonucleotides. As examples of such nanoparticles, tripodal sugar constituents of bleomycin were synthesized and conjugated with a fluorescence-labeled antisense oligonucleotide (AONARV7). Successive copper(I)-catalyzed azide-alkyne and strain-promoted alkyne-nitrone cycloadditions (SPANC) were utilized for the synthesis. Then, the glyco-AONARV7 conjugates were hybridized with complementary strands of a C60-based molecular spherical nucleic acid (i.e., a hybridization-mediated carrier). The formation and stability of these assembled glyco-decorated SNAs were evaluated by polyacrylamide gel electrophoresis (PAGE), UV melting profile analysis, and time-resolved fluorescence spectroscopy. Association constants were extracted from time-resolved fluorescence data. Preliminary cellular uptake experiments of the glyco-AONARV7 conjugates (120 nM solutions) and of the corresponding glycodecorated SNAs (10 nM solutions) with human prostate cancer cells (PC3) showed an efficient uptake in each case. A marked variation in intracellular distribution was observed.Peer reviewe

Nuorten liikuntakäyttäytyminen Suomessa : LIITU-tutkimuksen tuloksia 2020

nonPeerReviewe