Promesa, fe y alianza en gn 15,1-21 : acercamiento existencial mediante un análisis narrativo del texto

Esta monografía es una interpretación de Gn 15 que quiere mostrar cómo el análisis narrativo de Gn 15 permite entrar al lector contemporáneo en un cuestionamiento sobre su existencia. El primer capítulo es un estudio básico del texto, en el marco general de la crítica textual, para estar más seguro del texto original, su traducción, sus límites, su unidad y su estructura. El capítulo segundo consiste en un análisis narrativo, para determinar cuál es el mensaje central del texto, y cómo se transmite al lector. El tercer capítulo, se intenta determinar el interés del texto para el lector judío de la época post-exílica, así como para el lector contemporáneo. Esta parte reflexiona sobre el significado contemporáneo de las categorías de promesa, fe y alianza para expresar el mensaje existencial de Gn 15 para hoy.This interpretation of Gen 15, tries to show how narrative analysis permits an existential questioning of the contemporary reader. The first chapter is a basic study of the text, in the general framework of the textual criticism, to ensure the original text, his translation, limits, unity and structure. The second chapter consists in a narrative analysis, to determine the central message of the text and how it is transmitted to the reader. In the third chapter the investigation tries to determine the interest of the text for a postexilic Jew reader and for a contemporary one. This part considers the contemporary meaning of promise, faith and covenant categories to express today’s existential message of Gn 15.Magíster en TeologíaMaestrí

Thioredoxin-regulated β-amylase (BAM1) triggers diurnal starch degradation in guard cells, and in mesophyll cells under osmotic stress

BAM1 is a plastid-targeted β-amylase of Arabidopsis thaliana specifically activated by reducing conditions. Among eight different chloroplast thioredoxin isoforms, thioredoxin f1 was the most efficient redox mediator, followed by thioredoxins m1, m2, y1, y2, and m4. Plastid-localized NADPH-thioredoxin reductase (NTRC) was also able partially to restore the activity of oxidized BAM1. Promoter activity of BAM1 was studied by reporter gene expression (GUS and YFP) in Arabidopsis transgenic plants. In young (non-flowering) plants, BAM1 was expressed both in leaves and roots, but expression in leaves was mainly restricted to guard cells. Compared with wild-type plants, bam1 knockout mutants were characterized by having more starch in illuminated guard cells and reduced stomata opening, suggesting that thioredoxin-regulated BAM1 plays a role in diurnal starch degradation which sustains stomata opening. Besides guard cells, BAM1 appears in mesophyll cells of young plants as a result of a strongly induced gene expression under osmotic stress, which is paralleled by an increase in total β-amylase activity together with its redox-sensitive fraction. Osmotic stress impairs the rate of diurnal starch accumulation in leaves of wild-type plants, but has no effect on starch accumulation in bam1 mutants. It is proposed that thioredoxin-regulated BAM1 activates a starch degradation pathway in illuminated mesophyll cells upon osmotic stress, similar to the diurnal pathway of starch degradation in guard cells that is also dependent on thioredoxin-regulated BAM1