A Pilot Study on Real-time Monitoring of Heart Rate and Movement Speed in Middle-distance Race of Physical Education Classes

In Chinese universities, students need to participate in the middle-distance-race. Normally, female students are required to participate in the race of 800 meters, while male students are required to participate in the race of 1000 meters. However, it is difficult for teachers to grasp the real time information of students during the race. And there is a lack of timely communications between the teachers and students. Focusing on this issue, this study, with the use of POLAR heart rate sensor and other modern information technologies, expands the original function of the sensor to achieve a concurrent operation of detecting heart rates and automatically measuring the movement speed. The researchers have successfully designed a micro system to monitor the process of middle-distance race. Moreover, the study also engages in a preliminary experiment verification so as to provide object and effective reference and basis for the middle-distance race physical education teaching in universities

Oocyte stage-specific effects of MTOR determine granulosa cell fate and oocyte quality in mice.

MTOR (mechanistic target of rapamycin) is a widely recognized integrator of signals and pathways key for cellular metabolism, proliferation, and differentiation. Here we show that conditional knockout (cKO) of Mtor in either primordial or growing oocytes caused infertility but differentially affected oocyte quality, granulosa cell fate, and follicular development. cKO of Mtor in nongrowing primordial oocytes caused defective follicular development leading to progressive degeneration of oocytes and loss of granulosa cell identity coincident with the acquisition of immature Sertoli cell-like characteristics. Although Mtor was deleted at the primordial oocyte stage, DNA damage accumulated in oocytes during their later growth, and there was a marked alteration of the transcriptome in the few oocytes that achieved the fully grown stage. Although oocyte quality and fertility were also compromised when Mtor was deleted after oocytes had begun to grow, these occurred without overtly affecting folliculogenesis or the oocyte transcriptome. Nevertheless, there was a significant change in a cohort of proteins in mature oocytes. In particular, down-regulation of PRC1 (protein regulator of cytokinesis 1) impaired completion of the first meiotic division. Therefore, MTOR-dependent pathways in primordial or growing oocytes differentially affected downstream processes including follicular development, sex-specific identity of early granulosa cells, maintenance of oocyte genome integrity, oocyte gene expression, meiosis, and preimplantation developmental competence. Proc Natl Acad Sci U S A 2018 Jun 5; 115(23):E5326-E5333

Plexin-B1 silencing inhibits ovarian cancer cell migration and invasion

BACKGROUND: Elevated Plexin-B1 expression has been found in diverse human cancers and in non-neoplastic tissues, and it mediates diverse biological and pathological activities. However, whether or not Plexin-B1 expression is involved in human ovarian tumors remains unclear. In the present study, Plexin-B1 expression was explored in benign and malignant human ovarian tumor tissues. In addition, the impact of Plexin-B1 expression on ovarian cancer cell proliferation, migration and invasion were investigated in vitro. METHODS: Plexin-B1 expression was analyzed in normal and benign ovarian tissues and serous ovarian tumors (both borderline and malignant) by immunohistochemical staining, as well as in four human ovarian cancer cell lines (A2780, C13*, SKOV3, and OV2008) by RT-PCR and western blot analyses. Furthermore, endogenous Plexin-B1 expression was suppressed by Plexin-B1 siRNA in SKOV3 cells, which overexpress Plexin-B1. Protein levels of Plexin-B1, AKT and AKT(Ser473 )were examined by western blot analysis. Cell proliferation, migration and invasion were measured with MTT, wound healing and boyden chamber assays, respectively, and the cytoskeleton was monitored via F-actin staining. RESULTS: Expression levels of Plexin-B1 protein were significantly higher in serous ovarian carcinomas than in normal ovaries or benign ovarian neoplasms, and in the former, Plexin-B1 expression was positively correlated with lymphatic metastasis, and the membrane and cytoplasm of cancer cells stained positively. SKOV3 cells displayed the highest Plexin-B1 expression at both the mRNA and protein levels among the four tested human ovarian cancer cell lines and was selected as a cell model for further in vitro experiments. Plexin-B1 siRNA significantly suppressed phosphorylation of AKT at Ser473 in SKOV3 cells, but it did not alter total AKT expression. In addition, silencing of Plexin-B1 in SKOV3 cells inhibited cell migration and invasion and reorganized the cytoskeleton, whereas cell proliferation was not affected. CONCLUSION: Plexin-B1 expression correlates with malignant phenotypes of serous ovarian tumors, probably via phosphorylation of AKT at Ser473, suggesting that Plexin-B1 might be a useful biomarker and/or a novel therapeutic target

A Recombinant Vaccine of H5N1 HA1 Fused with Foldon and Human IgG Fc Induced Complete Cross-Clade Protection against Divergent H5N1 Viruses

Development of effective vaccines to prevent influenza, particularly highly pathogenic avian influenza (HPAI) caused by influenza A virus (IAV) subtype H5N1, is a challenging goal. In this study, we designed and constructed two recombinant influenza vaccine candidates by fusing hemagglutinin 1 (HA1) fragment of A/Anhui/1/2005(H5N1) to either Fc of human IgG (HA1-Fc) or foldon plus Fc (HA1-Fdc), and evaluated their immune responses and cross-protection against divergent strains of H5N1 virus. Results showed that these two recombinant vaccines induced strong immune responses in the vaccinated mice, which specifically reacted with HA1 proteins and an inactivated heterologous H5N1 virus. Both proteins were able to cross-neutralize infections by one homologous strain (clade 2.3) and four heterologous strains belonging to clades 0, 1, and 2.2 of H5N1 pseudoviruses as well as three heterologous strains (clades 0, 1, and 2.3.4) of H5N1 live virus. Importantly, immunization with these two vaccine candidates, especially HA1-Fdc, provided complete cross-clade protection against high-dose lethal challenge of different strains of H5N1 virus covering clade 0, 1, and 2.3.4 in the tested mouse model. This study suggests that the recombinant fusion proteins, particularly HA1-Fdc, could be developed into an efficacious universal H5N1 influenza vaccine, providing cross-protection against infections by divergent strains of highly pathogenic H5N1 virus

Elastic Inflatable Actuators for Soft Robotic Applications

The 20th century’s robotic systems have been made out of stiff materials and much of the developments in the field have pursued ever more accurate and dynamic robots which thrive in industrial automation settings and will probably continue to do so for many decades to come. However, the 21st century’s robotic legacy may very well become that of soft robots. This emerging domain is characterized by continuous soft structures that simultaneously fulfil the role of robotic link and robotic actuator, where prime focus is on design and fabrication of the robotic hardware instead of software control to achieve a desired operation. These robots are anticipated to take a prominent role in delicate tasks where classic robots fail, such as in minimally invasive surgery, active prosthetics and automation tasks involving delicate irregular objects. Central to the development of these robots is the fabrication of soft actuators to generate movement. This paper reviews a particularly attractive type of soft actuators that are driven by pressurized fluids. These actuators have recently gained substantial traction on the one hand due to the technology push from better simulation tools and new manufacturing technologies including soft-lithography and additive manufacturing, and on the other hand by a market pull from the applications listed above. This paper provides an overview of the different advanced soft actuator configurations, their design, fabrication and applications.This research is supported by the Fund for Scientific Research-Flanders (FWO), and the European Research Council (ERC starting grant HIENA)

梁漱溟乡村建设中心的当代实践 = Introduction of Liang Shuming Rural Reconstruction Center

梁漱溟乡村建设的核心思想是人的建设和社会的重建。在过去的20年里，一批年轻人在梁漱溟等先人思想的感召下，面对全球化带来的一些列社会问题的挑战，全身心地投入到乡村重建的工作中，从90年支持农民的维权行动，发展到2000年开展综合的新农村建设，坚持不懈地直到今天的生态文明建设的工作。梁漱溟乡村建设中心一直积极地参与到这些工作中，尤其是在组织大学生支农调研维权，到支持农民合作组织来建设农村的自治组织，通过社区支持农业来建构城市和农村互相支持的平台等方面发挥了重要的作用。 在多年的实践中，梁漱溟乡村建设中心意识到要实现乡村建设的目标是需要一个长期的努力过程，尤其是在培养人才，进而形成内生的自我解决社会问题的力量。在这个过程中，如何协助参与这个过程的人群的学习，不仅是我们自己，还有我们的合作伙伴-农民，作为主体能够意识到自身所处的小乡村的命运与大社会变革的关系，进而转化为主动型维护自身阶层利益的能力，是非常重要的，也是我们目前所面对的巨大挑战。 Liang Shuming’s core philosophy for rural reconstruction is human reconstruction and social reconstruction. In the past 20 years, a group of people, who believe the philosophy of rural reconstruction, have taken the challenges of globalization through supporting farmers’ legal appeal to protect their rights in the 1990s, supporting government’ new socialist rural reconstruction program in 2000s, and further to build ecological civilization in the society in 2010s. Liang Shuming Rural Reconstruction Center has actively participated in these three phases and has played key roles in the process of college students’ re-education by organizing rural research for farmers’ right protection, rural institutional establishment by supporting farmer cooperative formation and mutual support mechanism establishment between urban and rural people by promoting community support agriculture. Liang Shuming Center has realized that to fulfill the rural reconstruction goals requires a consistent efforts and long process for human development and further to address social issues by the social force developed from within. In this process, how to facilitate the learning process of farmers to realize the relationship between current local situation and global or national reform and further transform into their own initiatives to protect their own right and interests is core challenges Liang Shuming Center is facing

High-Permeability Wood with Microwave Remodeling Structure

This paper presents the development of a microwave puffed wood (MPW) with novel and unique structural features in its internal structure that are based on natural wood (NW). The focus of the research was on the comprehensive visualization of the structural characteristics of MPW from the macroscopic to the microscopic scale followed by an exploration of its impregnation capabilities. The results showed that the volume of MPW increased by about 9% compared to NW due to the presence of a large number of cracked cavities. The CT images indicated that there was a significant difference between the macroscopic cracks produced by microwave processing and the natural cracks in the wood. The mercury intrusion test results showed that macro-pores increased while the micro-pores decreased in the MPW compared to in the NW. The MPW showed good fluid permeability and liquid absorption performance. The phenolic resin penetration rate of the MPW was about 20 times that of the NW, and the material absorption was more than 2 times that of the NW. The crack space enabled the MPW to serve as a fluid transportation and a storage warehouse. MPW is a super container based on natural materials. It has broad potential in more fields, such as in wood composite materials