Chen, Zihao

Cruz-Reyes, Jorge

Goodall, Zachary

Ivens, Alasdair

McCleskey, Laura

McDermott, Suzanne M

Meehan, Joshua

Rodshagen, Tyler

Rouskin, Silvi

Savill, Nicholas Jon

Schnaufer, Achim

Seachrist, Rebecca

Stuart, Kenneth D

Woo, Jia

Yu, Zihao

Zeng, Lanying

Zhang, Xiuren

English

U-insertion/deletion (U-indel) RNA editing in trypanosome mitochondria is directed by guide RNAs (gRNAs). This editing may developmentally control respiration in bloodstream forms (BSF) and insect procyclic forms (PCF). Holo-editosomes include the accessory RNA Editing Substrate Binding Complex (RESC) and RNA Editing Helicase 2 Complex (REH2C), but the specific proteins controlling differential editing remain unknown. Also, RNA editing appears highly error prone because most U-indels do not match the canonical pattern. However, despite extensive non-canonical editing of unknown functions, accurate canonical editing is required for normal cell growth. In PCF, REH2C controls editing fidelity in RESC-bound mRNAs. Here, we report that KREH2, a REH2C-associated helicase, developmentally controls programmed non-canonical editing, including an abundant 3' element in ATPase subunit 6 (A6) mRNA. The 3' element sequence is directed by a proposed novel regulatory gRNA. In PCF, KREH2 RNAi-knockdown up-regulates the 3' element, which establishes a stable structure hindering element removal by canonical initiator-gRNA-directed editing. In BSF, KREH2-knockdown does not up-regulate the 3' element but reduces its high abundance. Thus, KREH2 differentially controls extensive non-canonical editing and associated RNA structure via a novel regulatory gRNA, potentially hijacking factors as a 'molecular sponge'. Furthermore, this gRNA is bifunctional, serving in canonical CR4 mRNA editing whilst installing a structural element in A6 mRNA.</p

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Trypanosome RNA helicase KREH2 differentially controls noncanonical editing and putative repressive structure via a novel proposed “bifunctional” gRNA in mRNA A6

https://www.research.ed.ac.uk/files/353452655/gkad453.pdf

Trypanosome RNA helicase KREH2 differentially controls noncanonical editing and putative repressive structure via a novel proposed “bifunctional” gRNA in mRNA A6

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