On the asymptotic giant branch star origin of peculiar spinel grain OC2

Microscopic presolar grains extracted from primitive meteorites have extremely anomalous isotopic compositions revealing the stellar origin of these grains. The composition of presolar spinel grain OC2 is different from that of all other presolar spinel grains. Large excesses of the heavy Mg isotopes are present and thus an origin from an intermediate-mass (IM) asymptotic giant branch (AGB) star was previously proposed for this grain. We discuss the isotopic compositions of presolar spinel grain OC2 and compare them to theoretical predictions. We show that the isotopic composition of O, Mg and Al in OC2 could be the signature of an AGB star of IM and metallicity close to solar experiencing hot bottom burning, or of an AGB star of low mass (LM) and low metallicity suffering very efficient cool bottom processing. Large measurement uncertainty in the Fe isotopic composition prevents us from discriminating which model better represents the parent star of OC2. However, the Cr isotopic composition of the grain favors an origin in an IM-AGB star of metallicity close to solar. Our IM-AGB models produce a self-consistent solution to match the composition of OC2 within the uncertainties related to reaction rates. Within this solution we predict that the 16O(p,g)17F and the 17O(p,a)14N reaction rates should be close to their lower and upper limits, respectively. By finding more grains like OC2 and by precisely measuring their Fe and Cr isotopic compositions, it may be possible in the future to derive constraints on massive AGB models from the study of presolar grains.Comment: 10 pages, 8 figures, accepted for publication on Astronomy & Astrophysic

Cyclophilin40 isomerase activity is regulated by a temperature-dependent allosteric interaction with Hsp90

Cyclophilin 40 (Cyp40) comprises an N-terminal cyclophilin domain with peptidyl-prolyl isomerase (PPIase) activity and a C-terminal tetratricopeptide repeat (TPR) domain that binds to the C-terminal–EEVD sequence common to both heat shock protein 70 (Hsp70) and Hsp90. We show in the present study that binding of peptides containing the MEEVD motif reduces the PPIase activity by ∼30%. CD and fluorescence assays show that the TPR domain is less stable than the cyclophilin domain and is stabilized by peptide binding. Isothermal titration calorimetry (ITC) shows that the affinity for the–MEEVD peptide is temperature sensitive in the physiological temperature range. Results from these biophysical studies fit with the MD simulations of the apo and holo (peptide-bound) structures which show a significant reduction in root mean square (RMS) fluctuation in both TPR and cyclophilin domains when–MEEVD is bound. The MD simulations of the apo-protein also highlight strong anti-correlated motions between residues around the PPIase-active site and a band of residues running across four of the seven helices in the TPR domain. Peptide binding leads to a distortion in the shape of the active site and a significant reduction in these strongly anti-correlated motions, providing an explanation for the allosteric effect of ligand binding and loss of PPIase activity. Together the experimental and MD results suggest that on heat shock, dissociation of Cyp40 from complexes mediated by the TPR domain leads to an increased pool of free Cyp40 capable of acting as an isomerase/chaperone in conditions of cellular stress

Scaled solar tracks and isochrones in a large region of the Z-Y plane. II. From 2.5 to 20 solar masses

We extend our theoretical computations for low-mass stars to intermediate-mass and massive stars, for which few databases exist in the literature. Evolutionary tracks and isochrones are computed from 2.50 to 20 solar masses for agrid of 37 chemical compositions with metal content Z between 0.0001 and 0.070 and helium content Y between 0.23 and 0.40. Synthetic TP-AGB models allow stellar tracks and isochrones to be extended until the end of the thermal pulses along the AGB. We provide software tools for the bidimensional interpolation (in Y and Z) of the isochrones. We present tracks for scaled-solar abundances and the corresponding isochrones from very old ages down to about 10 million years. The extension of the blue loops and the instability strip of Cepheid stars are compared and the Cepheid mass-discrepancy is discussed. The location of red supergiants in the H-R diagram is in good agreement with the evolutionary tracks for masses from 10 to 20 solar masses. Tracks and isochrones are available in tabular form for the adopted grid of chemical compositions in the extended plane Z-Y in three photometric systems. An interactive web interface allows users to obtain isochrones of any chemical composition inside the provided Z-Y range and also to simulate stellar populations with different Y(Z) helium-to-metal enrichment laws.Comment: 17 pages, 18 figures, accepted for publication in A&

Evidence Map of Pancreatic Surgery–A living systematic review with meta-analyses by the International Study Group of Pancreatic Surgery (ISGPS)

Background: Pancreatic surgery is associated with considerable morbidity and, consequently, offers a large and complex field for research. To prioritize relevant future scientific projects, it is of utmost importance to identify existing evidence and uncover research gaps. Thus, the aim of this project was to create a systematic and living Evidence Map of Pancreatic Surgery. Methods: PubMed, the Cochrane Central Register of Controlled Trials, and Web of Science were systematically searched for all randomized controlled trials and systematic reviews on pancreatic surgery. Outcomes from every existing randomized controlled trial were extracted, and trial quality was assessed. Systematic reviews were used to identify an absence of randomized controlled trials. Randomized controlled trials and systematic reviews on identical subjects were grouped according to research topics. A web-based evidence map modeled after a mind map was created to visualize existing evidence. Meta-analyses of specific outcomes of pancreatic surgery were performed for all research topics with more than 3 randomized controlled trials. For partial pancreatoduodenectomy and distal pancreatectomy, pooled benchmarks for outcomes were calculated with a 99% confidence interval. The evidence map undergoes regular updates. Results: Out of 30, 860 articles reviewed, 328 randomized controlled trials on 35, 600 patients and 332 systematic reviews were included and grouped into 76 research topics. Most randomized controlled trials were from Europe (46%) and most systematic reviews were from Asia (51%). A living meta-analysis of 21 out of 76 research topics (28%) was performed and included in the web-based evidence map. Evidence gaps were identified in 11 out of 76 research topics (14%). The benchmark for mortality was 2% (99% confidence interval: 1%–2%) for partial pancreatoduodenectomy and <1% (99% confidence interval: 0%–1%) for distal pancreatectomy. The benchmark for overall complications was 53% (99%confidence interval: 46%–61%) for partial pancreatoduodenectomy and 59% (99% confidence interval: 44%–80%) for distal pancreatectomy. Conclusion: The International Study Group of Pancreatic Surgery Evidence Map of Pancreatic Surgery, which is freely accessible via www.evidencemap.surgery and as a mobile phone app, provides a regularly updated overview of the available literature displayed in an intuitive fashion. Clinical decision making and evidence-based patient information are supported by the primary data provided, as well as by living meta-analyses. Researchers can use the systematic literature search and processed data for their own projects, and funding bodies can base their research priorities on evidence gaps that the map uncovers. © 2021 The Author

Cortical Thinning in Patients with Recent Onset Post-Traumatic Stress Disorder after a Single Prolonged Trauma Exposure

Most of magnetic resonance imaging (MRI) studies about post-traumatic stress disorder (PTSD) focused primarily on measuring of small brain structure volume or regional brain volume changes. There were rare reports investigating cortical thickness alterations in recent onset PTSD. Recent advances in computational analysis made it possible to measure cortical thickness in a fully automatic way, along with voxel-based morphometry (VBM) that enables an exploration of global structural changes throughout the brain by applying statistical parametric mapping (SPM) to high-resolution MRI. In this paper, Laplacian method was utilized to estimate cortical thickness after automatic segmentation of gray matter from MR images under SPM. Then thickness maps were analyzed by SPM8. Comparison between 10 survivors from a mining disaster with recent onset PTSD and 10 survivors without PTSD from the same trauma indicates cortical thinning in the left parietal lobe, right inferior frontal gyrus, and right parahippocampal gyrus. The regional cortical thickness of the right inferior frontal gyrus showed a significant negative correlation with the CAPS score in the patients with PTSD. Our study suggests that shape-related cortical thickness analysis may be more sensitive than volumetric analysis to subtle alteration at early stage of PTSD

METALLIC ION DEVELOPMENTS AT GANIL

Radioactive ion beams (RIB) are routinely produced at GANIL by fragmentation of the projectile. A possible way to improve the RIB intensity is to increase the primary beam intensity impinging the target. Although high intensities can be obtained with an ECR ion source for gaseous elements, it is more difficult for metallic elements due to the poor ionization efficiency of the source. This report deals with metallic ion beam production at high intensity. Experimental results for Ca, Ni and Fe are presented. The oven and the MIVOC methods are compared

Protein–Protein Interactions Modulate the Docking-Dependent E3-Ubiquitin Ligase Activity of Carboxy-Terminus of Hsc70-Interacting Protein (CHIP)

CHIP is a tetratricopeptide repeat (TPR) domain protein that functions as an E3-ubiquitin ligase. As well as linking the molecular chaperones to the ubiquitin proteasome system, CHIP also has a docking-dependent mode where it ubiquitinates native substrates, thereby regulating their steady state levels and/or function. Here we explore the effect of Hsp70 on the docking-dependent E3-ligase activity of CHIP. The TPR-domain is revealed as a binding site for allosteric modulators involved in determining CHIP's dynamic conformation and activity. Biochemical, biophysical and modeling evidence demonstrate that Hsp70-binding to the TPR, or Hsp70-mimetic mutations, regulate CHIP-mediated ubiquitination of p53 and IRF-1 through effects on U-box activity and substrate binding. HDX-MS was used to establish that conformational-inhibition-signals extended from the TPR-domain to the U-box. This underscores inter-domain allosteric regulation of CHIP by the core molecular chaperones. Defining the chaperone-associated TPR-domain of CHIP as a manager of inter-domain communication highlights the potential for scaffolding modules to regulate, as well as assemble, complexes that are fundamental to protein homeostatic control.Published versio