Model studies on the interactions of a Cu(II)-quinone complex with surfactant micelles and DNA explore its induction of apoptosis in human MDA-MB-231 breast adenocarcinoma cells

<p>The interactions of copper complex (CuQ₂) of 1-amino-4-hydroxy-9,10-anthraquinone (QH) with calf thymus DNA, anionic surfactant sodium dodecyl sulfate (SDS), and cationic surfactant cetyltrimethylammonium bromide (CTAB) were investigated in an aqueous solution at physiological pH (7.4). Affinities of such molecule to DNA and surfactant micelles, a model for a biological membrane, are important in determining its biological action. Using different models, various binding parameters were evaluated in both of molecule–DNA interaction and molecule–surfactant interaction. The study showed that hydrophobic interaction plays a major role in the binding of CuQ₂ to surfactant micelles. In addition, the hydrophobic interaction has an important role in the distribution of CuQ₂ between micelle-water phases. Gibbs free energy for the binding and distribution of CuQ₂ between the bulk aqueous medium and surfactant micelles were calculated. In order to correlate the physicochemical properties deciphered from the aforementioned studies with the biological property of the molecule, CuQ₂ was treated with MDA-MB-231 breast adenocarcinoma cells where it was found that the molecule affects the viability of the cancer cells. Fluorescent staining of the treated cells with AO/EB and Hoechst indicated that the CuQ₂ induces apoptosis, suggesting its use in the treatment of breast cancer.</p

Draft Genome Sequence of Staphylococcus aureus ST672, an Emerging Disease Clone from India

We report the draft genome sequence of methicillin-resistant Staphylococcus aureus (MRSA) strain ST672, an emerging disease clone in India, from a septicemia patient. The genome size is about 2.82 Mb with 2,485 open reading frames (ORFs). The staphylococcal cassette chromosome mec (SCCmec) element (type V) and immune evasion cluster appear to be different from those of strain ST772 on preliminary examination

Draft Genome Sequence of Staphylococcus aureus 118 (ST772), a Major Disease Clone from India

We report the draft genome sequence of an ST772 Staphylococcus aureus disease isolate carrying staphylococcal cassette chromosome mec (SCCmec) type V from a pyomyositis patient. Our de novo short read assembly is similar to 2.8 Mb and encodes a unique Panton-Valentine leukocidin (PVL) phage with structural genes similar to those of phi 7247PVL and novel lysogenic genes at the N termini

Synthesis, crystal structure, catecholase activity, DNA cleavage and anticancer activity of a dinuclear manganese(III)-bipyridine complex

170-178Design and synthesis of a dinuclear manganese(III) complex [Mn2(µ-O)<span style="mso-bidi-font-size: 9.0pt;mso-ansi-language:EN-IN;mso-fareast-language:ZH-TW">(<i style="mso-bidi-font-style: normal">μ-O2CMe)<span style="mso-bidi-font-size: 9.0pt" lang="EN-GB">2(H2O)2(2,2'-bipy)2](NO3)2.5H2O (1) [2,2'-bipy = 2,2'-bipyridine] with mono-<span style="mso-bidi-font-size:9.0pt;color:black; layout-grid-mode:line" lang="EN-GB">-oxo and di-<span style="mso-bidi-font-size: 9.0pt;font-family:Symbol;mso-ascii-font-family:" times="" new="" roman";mso-hansi-font-family:="" "times="" roman";color:black;layout-grid-mode:line;mso-char-type:symbol;="" mso-symbol-font-family:symbol"="" lang="EN-GB">-acetato bridge has been reported. Single crystal X-ray diffraction study reveals that (1) crystallizes in monoclinic system with P21/<i style="mso-bidi-font-style: normal">n space group. Evaluation of (<b style="mso-bidi-font-weight: normal">1)<span style="mso-ansi-language:EN-IN; mso-fareast-language:EN-IN" lang="EN-GB"> <span style="mso-fareast-font-family:AdvPS_TTR; mso-ansi-language:EN-IN;mso-fareast-language:ZH-TW">as a model system for the catechol oxidase enzyme by using 3,5-di-tert-butylcatechol (DTBC) as the substrate in methanol medium, reveals that the complex <span style="mso-fareast-font-family:AdvPS_TTR;mso-ansi-language:EN-IN;mso-fareast-language: ZH-TW">exhibits efficient catalytic activity with <i style="mso-bidi-font-style: normal">kcat value of 8.89×102<span style="mso-fareast-font-family:AdvTimes;mso-ansi-language:EN-IN;mso-fareast-language: EN-IN">. (1) cleaves the <span style="mso-fareast-font-family:AdvGulliv-R;mso-ansi-language:EN-IN;mso-fareast-language: EN-IN">pBR 322 <span style="mso-ansi-language:EN-IN;mso-fareast-language: EN-IN">DNA without addition of an activating agent. Further, the anticancer activity of (1) on human hepatocarcinoma cell line (HepG2) has been examined. The apoptosis in the cell line has been assessed by the changes in cell morphology, which shows the efficacy of (1) to induce 55% of apoptotic for 24 h<span style="mso-ansi-language: EN-IN;mso-fareast-language:ZH-TW">. Interestingly, the observed IC50 values reveal that (1) effects conformational change on DNA strongly and exhibits remarkable cytotoxicity. </span