MHC antigens in interferon γ (IFNγ) receptor deficient mice: IFNγ-independent up-regulation of MHC class II in renal tubules

MHC antigens in interferon γ (IFNγ) receptor deficient mice: IFNγ-independent up-regulation of MHC class II in renal tubules. MHC class II gene products in parenchymal cells, such as tubular epithelial cells in kidney, may play a role in the regulation of autoimmune reactions. Expression of MHC class II in renal tubular cells is normally very low, but it increases considerably under various pathologic conditions. The predominant role of IFNγ in up-regulation of MHC class II expression has been demonstrated repeatedly. We tested the existence of alternative pathways of MHC class II regulation using IFNγ receptor-deficient (IFNγR-/-) mice. Mutant and wild type mice received 50 µg bacterial endotoxin (LPS) i.p. Four days later the kidneys were removed for immunofluorescence examination. In agreement with published results LPS provoked an increase of immunoreactivity for MHC class I and MHC class II in proximal tubules of wild type mice. While MHC class I up-regulation was strictly IFNγ receptor-dependent, up-regulation of MHC II was still evident in mutant mice, although less than in wild type mice. Since injection of IFNγ induced proximal tubular MHC class II expression in wild type mice but not in IFNγR-/- mice, an alternative signaling pathway for IFNγ does not seem to exist. Thus, up-regulation of MHC class II expression in renal tubules does not necessarily require IFNγ. The markedly patchy pattern of immunofluorescence in IFNγR-/- mice suggests that induction of MHC class II after LPS injection may represent renal injury due to shock

Broad band flux-density monitoring of radio sources with the Onsala twin telescopes

The Onsala twin telescopes (OTT) are two 13 m telescopes located at the Onsala Space Observatory in Sweden. With dual linear polarized broad-band (3-14 GHz) receivers, they are part of the next generation Very Long Baseline Interferometry (VLBI) Global Observing System (VGOS) for geodesy and astrometry. In addition to purely geodetic data products, VGOS will regularly produce full-polarisation images of hundreds of radio sources. These rich monitoring data will be valuable for both astronomy and geodesy. In this pilot study we aimed at monitoring ten bright radio sources to search for flares or similar activity, and at testing the instrument calibration on long (months) and short (hours) time scales. Using the OTT as a standalone instrument, we observed and analysed 91 short (<30 min) sessions spanning seven months. We monitored seven potentially variable radio sources (0059+581, 0552+398, 1144+402, 1156+295, 1617+229, 3C418, OJ287) and three reference calibrators (3C147, 3C286, 3C295). We used the Common Astronomy Software Applications (CASA) package to fringe-fit, bandpass-correct and scale the data to obtain flux densities in the four standard VGOS bands: 3.0-3.5 GHz (band 1), 5.2-5.7 GHz (band 2), 6.3-6.8 GHz (band 3), and 10.2-10.7 GHz (band 4). We obtained simultaneous multi-frequency light curves for ten radio sources. A bright multi-frequency flare is observed in the radio source 0059+581. OJ287 and 1156+295 show significant long-term variability in their light curves. After correcting for instrumental biases, we determine the empirical flux density uncertainty as similar to 5 %

Uniaxial mechanical properties of face-centered cubic single- and multiphase high-entropy alloys

Since the high entropy concept was proposed at the beginning of the millennium, the research focus of this alloy family has been wide ranging. The initial search for single-phase alloys has expanded with the aim of improving mechanical properties. This can be achieved by several strengthening mechanisms such as solid-solution hardening, hot and cold working and precipitation hardening. Both single- and multiphase high- and medium-entropy alloys can be optimized for mechanical strength via several processing routes, as is the case for conventional alloys with only one base element, such as steels or Ni-based superalloys

Global Climate Services: A Typology of Global Decisions Influenced by Climate Risk

Climate services are ideally co-developed by scientists and stakeholders working together to identify decisions and user needs. Yet, while climate services have been developed at regional to local scales, relatively little attention has been paid to the global scale. Global climate services involve decisions that rely on climate information from many locations in different world regions, and are increasingly salient. Increasing interconnections in the global financial system and supply chains expose private companies and financial institutions to climate risk in multiple locations in different world regions. Further, multilateral decisions on greenhouse gas emission reduction targets, disaster risk finance or international migration should make use of global scale climate risk assessments. In order to advance global climate service development, we present a typology of decisions relying on global (i.e., non-local) climate risk information. We illustrate each decision type through examples of current practice from the coastal domain drawn from the literature and stakeholder interviews. We identify 8 types of decisions making use of global climate information. At a top-level, we distinguish between “multilateral climate policy decisions,” and “portfolio decisions involving multiple locations.” Multilateral climate policy decisions regard either “mitigation targets” or “multilateral adaptation” decisions. Portfolio decisions regard either “choice of location” or “choice of financial asset” decisions. Choice of location decisions can be further distinguished as to whether they involve “direct climate risks,” “supply chain risks” or “financial network risks.” Our survey of examples shows that global climate service development is more advanced for portfolio decisions taken by companies with experience in climate risk assessment, i.e., (re-)insurers, whereas many multilateral climate policy decisions are at an earlier stage of decision-making. Our typology thus provides an entry-point for global climate service development by pointing to promising research directions for supporting global (non-local) decisions that account for climate risks.Peer Reviewe

Semi-pilot scale-up of a continuous packed-bed bioreactor system developed for the lipase-catalyzed production of pseudo-ceramides

Ceramides are sphingolipid compounds that are very attractive as active components in both the pharmaceutical and the cosmetic industries. In this study, the synthesis of 1-O,3-N-diacyl 3-amino-1,2-propanediol-type pseudo-ceramides was developed at the semi-pilot scale, starting from a two-step continuous enzymatic process with immobilized Candida antarctica lipase B (Novozym® 435) in a packed-bed bioreactor, previously optimized at the laboratory scale. This process involved the selective N-acylation of 3-amino-1,2-propanediol (step 1), followed by the selective O-acylation of the N-acyl 3-amino-1,2-propanediol synthesized in the first step, with various fatty acids as acyl donors, to produce N,O-diacyl 3-amino-1,2-propanediol-type pseudo-ceramides (step 2). Under partially optimized operating conditions, high synthesis yields and production rates were obtained, within the ranges 76–92% and 3.7–4.6 g h−1 (step 1), or 23–36% and 1–1.4 g h−1 (step 2), respectively, depending on the fatty acids used as acyl donors. The overall synthesis yields varied from 20 to 33%: the best yield was obtained using palmitic acid and lauric acid as first and second acyl donors, respectively. Together with the high production rates also obtained with these acyl donors, this confirms that this two-step process has great potential for the production of differently functionalized 1-O,3-N-diacyl 3-amino-1,2-propanediol-type pseudo-ceramides on an industrial scale

Advancing tuna catch allocation negotiations: an analysis of sovereign rights and fisheries access arrangements

Regional fisheries management organisations (RFMOs) determine conservation measures for transboundary fsheries resources. They are also a forum for collective action toward the management of marine resources. One of the most complex and controversial aspects of this process is the allocation of catches between RFMO members. There are a variety of processes that can be used for catch allocation. In recent years, there has been a trend in some RFMOs towards establishing a system of criteria or indicators to determine the volume or percentage of catch that should be allocated to each RFMO member. Establishing such a system is challenging and the position of countries negotiating at RFMOs is also shaped by fisheries access arrangements. The debate on allocation has been ongoing at the Indian Ocean Tuna Commission for more than a decade, where one key point of disagreement is the treatment of historical catch taken in the waters of a coastal State. On the one hand, coastal states claim that catches historically taken in their exclusive economic zones (EEZ) should be attributed to them based on their sovereign rights over living resources. On the other, some fishing countries from outside the region claim catch history based on fishing conducted in the coastal State’s waters pursuant to access agreements. We analysed UNCLOS articles, publicly available fisheries access agreements, and national legislation to unpack the linkage between fisheries access arrangements and catch allocation discussions, and we also explored examples from other regions and RFMOs. We point out that the sovereign rights of coastal states over their EEZ provide a strong basis for allocation negotiations. In the absence of specific agreements to the contrary, any catch history that arises from foreign vessels fishing inside the EEZ should be attributed to the coastal State. We also argue that it is time for members of RFMOs—and especially of the Indian Ocean Tuna Commission—to move beyond the historical catch debate. They need to resolve their differences or consider other ways to allocate participatory rights in shared fisheries

Minimally Invasive Approaches for the Management of “Difficult” Colonic Polyps

Traditionally, patients with colonic polyps not amenable to endoscopic removal require open colectomy for management. We evaluated our experience with minimally invasive approaches including endoscopic mucosal resection (EMR), laparoscopic-assisted endoscopic polypectomy (LAEP), and laparoscopic-assisted colectomy (LAC). Patients referred for surgery for colonic polyps were selected for one of three minimally invasive modalities. A total of 123 patients were referred for resection of “difficult” polyps. Thirty underwent EMR, 25 underwent LAEP, and 68 underwent LAC. Of those selected to undergo EMR or LAEP, 76.4% were successfully managed without colon resection. The remaining 23.6% underwent LAC. Nine complications were encountered, including two requiring reoperative intervention. Of the 123 patients, three were found to have malignant disease on final pathology. Surgical resection can be avoided in a significant number of patients with “difficult” polyps referred for surgery by performing EMR and LAEP. In those who require surgery, minimally invasive resection can be achieved

The distribution of height and diameter in random non-plane binary trees

This study is dedicated to precise distributional analyses of the height of non-plane unlabelled binary trees ("Otter trees"), when trees of a given size are taken with equal likelihood. The height of a rooted tree of size $n$ is proved to admit a limiting theta distribution, both in a central and local sense, as well as obey moderate as well as large deviations estimates. The approximations obtained for height also yield the limiting distribution of the diameter of unrooted trees. The proofs rely on a precise analysis, in the complex plane and near singularities, of generating functions associated with trees of bounded height

Packing and Hausdorff measures of stable trees

In this paper we discuss Hausdorff and packing measures of random continuous trees called stable trees. Stable trees form a specific class of L\'evy trees (introduced by Le Gall and Le Jan in 1998) that contains Aldous's continuum random tree (1991) which corresponds to the Brownian case. We provide results for the whole stable trees and for their level sets that are the sets of points situated at a given distance from the root. We first show that there is no exact packing measure for levels sets. We also prove that non-Brownian stable trees and their level sets have no exact Hausdorff measure with regularly varying gauge function, which continues previous results from a joint work with J-F Le Gall (2006).Comment: 40 page