23 research outputs found
Characterisation of Phomopsis spp. associated with die-back of rooibos (Aspalathus linearis) in South Africa
Die-back of rooibos (Aspalathus linearis) causes substantial losses in commercial Aspalathus plantations in South Africa. In the past, the disease has been attributed to Phomopsis phaseoli (teleomorph: Diaporthe phaseolorum). Isolates obtained from diseased plants, however, were highly variable with regard to morphology and pathogenicity. The aim of the present study was thus to identify the Phomopsis species associated with die-back of rooibos. Isolates were subjected to DNA sequence comparisons of the internal transcribed spacer region (ITS1, 5.8S, ITS2) and partial sequences of the translation elongation factor-1 alpha gene. Furthermore, isolates were also compared in glasshouse inoculation trials on 8-mo-old potted plants to evaluate their pathogenicity. Five species were identified, of which D. aspalathi (formerly identified as D. phaseolorum or D. phaseolorum var. meridionalis) proved to be the most virulent, followed by D. ambigua, Phomopsis theicola, one species of Libertella and Phomopsis, respectively, and a newly described species, P. cuppatea. A description is also provided for D. ambigua based on a newly designated epitype specimen
The genus Bipolaris
The genus Bipolaris includes important plant pathogens with worldwide distribution. Species recognition in the genus has been uncertain due to the lack of molecular data from ex-type cultures as well as overlapping morphological characteristics. In this study, we revise the genus Bipolaris based on DNA sequence data derived from living cultures of fresh isolates, available ex-type cultures from worldwide collections and observation of type and additional specimens. Combined analyses of ITS, GPDH and TEF gene sequences were used to reconstruct the molecular phylogeny of the genus Bipolaris for species with living cultures. The GPDH gene is determined to be the best single marker for species of Bipolaris. Generic boundaries between Bipolaris and Curvularia are revised and presented in an updated combined ITS and GPDH phylogenetic tree. We accept 47 species in the genus Bipolaris and clarify the taxonomy, host associations, geographic distributions and species’ synonymies. Modern descriptions and illustrations are provided for 38 species in the genus with notes provided for the other taxa when recent descriptions are available. Bipolaris cynodontis, B. oryzae, B. victoriae, B. yamadae and B. zeicola are epi- or neotypified and a lectotype is designated for B. stenospila. Excluded and doubtful species are listed with notes on taxonomy and phylogeny. Seven new combinations are introduced in the genus Curvularia to accomodate the species of Bipolaris transferred based on the phylogenetic analysis. A taxonomic key is provided for the morphological identification of species within the genus
A systematic account of the genus Plagiostoma (Gnomoniaceae, Diaporthales) based on morphology, host-associations, and a four-gene phylogeny
Members of the genus Plagiostoma inhabit leaves, stems, twigs, and
branches of woody and herbaceous plants predominantly in the temperate
Northern Hemisphere. An account of all known species of Plagiostoma
including Cryptodiaporthe is presented based on analyses of
morphological, cultural, and DNA sequence data. Multigene phylogenetic
analyses of DNA sequences from four genes (β-tubulin, ITS,
rpb2, and tef1-α) revealed eight previously
undescribed phylogenetic species and an association between a clade composed
of 11 species of Plagiostoma and the host family Salicaceae.
In this paper these eight new species of Plagiostoma are described,
four species are redescribed, and four new combinations are proposed. A key to
the 25 accepted species of Plagiostoma based on host, shape, and size
of perithecia, perithecial arrangement in the host, and microscopic
characteristics of the asci and ascospores is provided. Disposition of
additional names in Cryptodiaporthe and Plagiostoma is also
discussed
Leaf-inhabiting genera of the Gnomoniaceae, Diaporthales
The Gnomoniaceae are characterised by ascomata that are generally
immersed, solitary, without a stroma, or aggregated with a rudimentary stroma,
in herbaceous plant material especially in leaves, twigs or stems, but also in
bark or wood. The ascomata are black, soft-textured, thin-walled, and
pseudoparenchymatous with one or more central or eccentric necks. The asci
usually have a distinct apical ring. The Gnomoniaceae includes
species having ascospores that are small, mostly less than 25 ÎĽm long,
although some are longer, and range in septation from non-septate to
one-septate, rarely multi-septate. Molecular studies of the
Gnomoniaceae suggest that the traditional classification of genera
based on characteristics of the ascomata such as position of the neck and
ascospores such as septation have resulted in genera that are not
monophyletic. In this paper the concepts of the leaf-inhabiting genera in the
Gnomoniaceae are reevaluated using multiple genes, specifically
nrLSU, translation elongation factor 1-alpha (tef1-α), and RNA
polymerase II second largest subunit (rpb2) for 64 isolates. ITS sequences
were generated for 322 isolates. Six genera of leaf-inhabiting
Gnomoniaceae are defined based on placement of their type species
within the multigene phylogeny. The new monotypic genus
Ambarignomonia is established for an unusual species, A.
petiolorum. A key to 59 species of leaf-inhabiting Gnomoniaceae is
presented and 22 species of Gnomoniaceae are described and
illustrated
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A mechanistic model of small intestinal starch digestion and glucose uptake in the cow
The high contribution of postruminal starch digestion (up to 50%) to total-tract starch digestion on energy-dense, starch-rich diets demands that limitations to small intestinal starch digestion be identified. A mechanistic model of the small intestine was described and evaluated with regard to its ability to simulate observations from abomasal carbohydrate infusions in the dairy cow. The 7 state variables represent starch, oligosaccharide, glucose, and pancreatic amylase in the intestinal lumen, oligosaccharide and glucose in the unstirred water layer at the intestinal wall, and intracellular glucose of the enterocyte. Enzymatic hydrolysis of starch was modeled as a 2-stage process involving the activity of pancreatic amylase in the lumen and of oligosaccharidase at the brush border of the enterocyte confined within the unstirred water layer. The Na+-dependent glucose transport into the enterocyte was represented along with a facilitative glucose transporter 2 transport system on the basolateral membrane. The small intestine is subdivided into 3 main sections, representing the duodenum, jejunum, and ileum for parameterization. Further subsections are defined between which continual digesta flow is represented. The model predicted nonstructural carbohydrate disappearance in the small intestine for cattle unadapted to duodenal infusion with a coefficient of determination of 0.92 and a root mean square prediction error of 25.4%. Simulation of glucose disappearance for mature Holstein heifers adapted to various levels of duodenal glucose infusion yielded a coefficient of determination of 0.81 and a root mean square prediction error of 38.6%. Analysis of model behavior identified limitations to the efficiency of small intestinal starch digestion with high levels of duodenal starch flow. Limitations to individual processes, particularly starch digestion in the proximal section of the intestine, can create asynchrony between starch hydrolysis and glucose uptake capacity
Names of Phytopathogenic Fungi: A Practical Guide
Using the correct name for phytopathogenic fungi and oomycetes is essential for communicating knowledge about species and their biology, control, and quarantine as well as for trade and research purposes. However, many plant pathogenic fungi are pleomorphic, meaning they produce different asexual (anamorph) and sexual (teleomorph) morphs in their life cycles. Therefore, more than one name has been applied to different morphs of the same species, which has confused users. The onset of DNA technologies makes it possible to connect different morphs of the same species, resulting in a move to a more natural classification system for fungi in which a single name for a genus and species can now be used. This move to a single nomenclature, coupled with the advent of molecular systematics and the introduction of polythetic taxonomic approaches, has been the main driving force for a reclassification of fungi, including pathogens. Nonetheless, finding the correct name for species remains challenging. In this article we outline a series of steps or considerations to greatly simplify this process and provide links to various online databases and resources to aid in determining the correct name. Additionally, a list of accurate names is provided for the most common genera and species of phytopathogenic fungi
Karnal bunt of wheat newly reported from the african continent
[No abstract available]Articl