Energetic and Environmental Constraints on the Community Structure of Benthic Microbial Mats in Lake Fryxell, Antarctica.

Ecological communities are regulated by the flow of energy through environments. Energy flow is typically limited by access to photosynthetically active radiation (PAR) and oxygen concentration (O2). The microbial mats growing on the bottom of Lake Fryxell, Antarctica, have well-defined environmental gradients in PAR and (O2). We analyzed the metagenomes of layers from these microbial mats to test the extent to which access to oxygen and light controls community structure. We found variation in the diversity and relative abundances of Archaea, Bacteria and Eukaryotes across three (O2) and PAR conditions: high (O2) and maximum PAR, variable (O2) with lower maximum PAR, and low (O2) and maximum PAR. We found distinct communities structured by the optimization of energy use on a millimeter-scale across these conditions. In mat layers where (O2) was saturated, PAR structured the community. In contrast, (O2) positively correlated with diversity and affected the distribution of dominant populations across the three habitats, suggesting that meter-scale diversity is structured by energy availability. Microbial communities changed across covarying gradients of PAR and (O2). The comprehensive metagenomic analysis suggests that the benthic microbial communities in Lake Fryxell are structured by energy flow across both meter- and millimeter-scales

Dynamic association between perfusion and white matter integrity across time since injury in Veterans with history of TBI.

ObjectiveCerebral blood flow (CBF) plays a critical role in the maintenance of neuronal integrity, and CBF alterations have been linked to deleterious white matter changes. Although both CBF and white matter microstructural alterations have been observed within the context of traumatic brain injury (TBI), the degree to which these pathological changes relate to one another and whether this association is altered by time since injury have not been examined. The current study therefore sought to clarify associations between resting CBF and white matter microstructure post-TBI.Methods37 veterans with history of mild or moderate TBI (mmTBI) underwent neuroimaging and completed health and psychiatric symptom questionnaires. Resting CBF was measured with multiphase pseudocontinuous arterial spin labeling (MPPCASL), and white matter microstructural integrity was measured with diffusion tensor imaging (DTI). The cingulate cortex and cingulum bundle were selected as a priori regions of interest for the ASL and DTI data, respectively, given the known vulnerability of these regions to TBI.ResultsRegression analyses controlling for age, sex, and posttraumatic stress disorder (PTSD) symptoms revealed a significant time since injury × resting CBF interaction for the left cingulum (p < 0.005). Decreased CBF was significantly associated with reduced cingulum fractional anisotropy (FA) in the chronic phase; however, no such association was observed for participants with less remote TBI.ConclusionsOur results showed that reduced CBF was associated with poorer white matter integrity in those who were further removed from their brain injury. Findings provide preliminary evidence of a possible dynamic association between CBF and white matter microstructure that warrants additional consideration within the context of the negative long-term clinical outcomes frequently observed in those with history of TBI. Additional cross-disciplinary studies integrating multiple imaging modalities (e.g., DTI, ASL) and refined neuropsychiatric assessment are needed to better understand the nature, temporal course, and dynamic association between brain changes and clinical outcomes post-injury

The Mass-Metallicity Relation at z~2

We use a sample of 87 rest-frame UV-selected star-forming galaxies with mean spectroscopic redshift z=2.26 to study the correlation between metallicity and stellar mass at high redshift. Using stellar masses determined from SED fitting to 0.3-8 micron photometry, we divide the sample into six bins in stellar mass, and construct six composite H-alpha+[NII] spectra from all of the objects in each bin. We estimate the mean oxygen abundance in each bin from the [NII]/H-alpha ratio, and find a monotonic increase in metallicity with increasing stellar mass, from 12+log(O/H) = 2.7e9 Msun to 12+log(O/H) = 8.6 for galaxies with = 1e11 Msun. We use the empirical relation between star formation rate density and gas density to estimate the gas fractions of the galaxies, finding an increase in gas fraction with decreasing stellar mass. These gas fractions combined with the observed metallicities allow the estimation of the effective yield y_eff as a function of stellar mass; in constrast to observations in the local universe which show a decrease in y_eff with decreasing baryonic mass, we find a slight increase. Such a variation of metallicity with gas fraction is best fit by a model with supersolar yield and an outflow rate ~4 times higher than the star formation rate. We conclude that the mass-metallicity relation at high redshift is driven by the increase in metallicity as the gas fraction decreases through star formation, and is likely modulated by metal loss from strong outflows in galaxies of all masses. There is no evidence for preferential loss of metals from low mass galaxies as has been suggested in the local universe. [Abridged]Comment: 18 pages, 9 figures, 2 tables; accepted for publication in Ap

Immunization of Mice with Anthrax Protective Antigen Limits Cardiotoxicity but Not Hepatotoxicity Following Lethal Toxin Challenge

Protective immunity against anthrax is inferred from measurement of vaccine antigen-specific neutralizing antibody titers in serum samples. In animal models, in vivo challenges with toxin and/or spores can also be performed. However, neither of these approaches considers toxin-induced damage to specific organ systems. It is therefore important to determine to what extent anthrax vaccines and existing or candidate adjuvants can provide organ-specific protection against intoxication. We therefore compared the ability of Alum, CpG DNA and the CD1d ligand alpha-galactosylceramide (alphaGC) to enhance protective antigen-specific antibody titers, to protect mice against challenge with lethal toxin, and to block cardiotoxicity and hepatotoxicity. By measurement of serum cardiac Troponin I (cTnI), and hepatic alanine aminotransferase (ALT), and aspartate aminotransferase (AST), it was apparent that neither vaccine modality prevented hepatic intoxication, despite high Ab titers and ultimate survival of the subject. In contrast, cardiotoxicity was greatly diminished by prior immunization. This shows that a vaccine that confers survival following toxin exposure may still have an associated morbidity. We propose that organ-specific intoxication should be monitored routinely during research into new vaccine modalities

Rapid HIV testing and counseling for residents in battered women’s shelters

Over one million Americans live with the human immunodeficiency virus (HIV), and roughly 20 percent of those living with HIV are unaware of their status. One way to decrease this epidemic is community-based rapid testing with high-risk populations. One high-risk population that has received limited attention is victims of intimate partner violence who seek shelter. In an effort to gain foundational information to implement rapid HIV testing and counseling services in domestic violence shelters, the current study conducted a series of focus groups with eighteen residents and ten staff of local shelters from October 15 to December 12, 2012. Participants provided valuable insight into how HIV rapid testing and counseling might be best implemented given the resources and constraints of shelter life. Despite identifying some potential barriers, most believed that the promise of quick results, the convenience and support afforded by the shelter venue, and the timing of the intervention at a point when women are making life changes would render the intervention acceptable to residents. Further insights are discussed in the article

Endogenous Gonadal Hormone Exposure and Bone Sarcoma Risk

Although experimental and clinical evidence suggest that endogenous sex hormones influence bone sarcoma genesis, the hypothesis has not been adequately tested in an appropriate animal model. We conducted a historical cohort study of Rottweiler dogs because they frequently undergo elective gonadectomy and spontaneously develop appendicular bone sarcomas, which mimic the biological behavior of the osteosarcomas that affect children and adolescents. Data were collected by questionnaire from owners of 683 Rottweiler dogs living in North America. To determine whether there was an association between endogenous sex hormones and risk of bone sarcoma, relative risk (RR) of incidence rates and hazard ratios for bone sarcoma were calculated for dogs subdivided on the basis of lifetime gonadal hormone exposure. Bone sarcoma was diagnosed in 12.6% of dogs in this cohort during 71,004 dog-months follow-up. Risk for bone sarcoma was significantly influenced by age at gonadectomy. Male and female dogs that underwent gonadectomy before 1 year of age had an approximate one in four lifetime risk for bone sarcoma and were significantly more likely to develop bone sarcoma than dogs that were sexually intact [RR ±95% CI = 3.8 (1.5–9.2) for males; RR ±95% CI = 3.1 (1.1–8.3) for females]. χ2 test for trend showed a highly significant inverse dose-response relationship between duration of lifetime gonadal exposure and incidence rate of bone sarcoma (P = 0.008 for males, P = 0.006 for females). This association was independent of adult height or body weight. We conclude that the subset of Rottweiler dogs that undergo early gonadectomy represent a unique, highly accessible target population to further study the gene:environment interactions that determine bone sarcoma risk and to test whether interventions can inhibit the spontaneous development of bone sarcoma

Inclusive partnership: Enhancing student engagement in geography

This is an Accepted Manuscript of an article published by Taylor & Francis in on , available online: http://wwww.tandfonline.com/10.1080/03098265.2015.1066316Partnership is currently the focus of much work within higher education (HEA, 2014; Healey et al., 2014; Cook-Sather et al., 2014) and advocated as an important process to address a range of higher education goals. In this paper, we propose the term inclusive partnership to conceptualise a non-selective staff-student relationship

Spectroscopic Confirmation of the Least Massive Known Brown Dwarf in Chamaeleon

We present spectroscopy of two candidate substellar members of the Chamaeleon I star-forming region. The candidates, which were identified photometrically by Oasa, Tamura, & Sugitani, have been observed at 1-2.5 micron during commissioning of the Gemini Near-Infrared Spectrograph. The late-type nature of one of the candidates, OTS 44, is confirmed through the detection of strong steam absorption bands. The other object, OTS 7, exhibits no late-type features and is likely a background star or galaxy. The gravity-sensitive shape of the H- and K-band continua demonstrate that OTS 44 is a young, pre-main-sequence object rather than a field dwarf. We measure a spectral type of M9.5 for OTS 44 based on a comparison of its spectrum to data for optically-classified young late-type objects. Because OTS 44 is the coolest and faintest object with confirmed membership in Chamaeleon I, it is very likely the least massive known member of the cluster. By comparing the position of OTS 44 on the H-R diagram to the evolutionary models of Chabrier & Baraffe, we infer a mass of ~0.015 M_sun. Although this estimate is uncertain by at least a factor of two, OTS 44 is nevertheless one of the least massive free-floating brown dwarfs confirmed spectroscopically to date.Comment: 10 pages, The Astrophysical Journal, 2004, v617 (December 10