Using the Kaban Lakes Integrated Assessment Model for Investigating Potential Levels of Antibiotic Pollution of the Nizhniy Kaban and Sredniy Kaban Lakes

Publisher's version (útgefin grein)The Kaban Lakes Integrated Assessment Model (KLIAM) was enhanced in order to assess the possible content of antibiotics in the Kaban lakes, located within the city borders of Kazan City, Tatarstan Republic in the Russian Federation, and potential for adverse environmental effects. The Kaban Lakes Integrated Assessment Model simulations suggest that the concentrations in the Nizhniy Kaban lake and Sredniy Kaban lake may exceed the predicted no effect concentration (PNEC) and low-risk limits set by EU and the WHO. Many missing data could be assumed or approximated, and simulation runs were conducted. The results are consistent with other global studies in terms of average concentrations observed elsewhere in rivers and lakes. The results suggest that the study should be followed up with lake water analysis and an assessment of antibiotic loads to the Kaban lakes. It is concluded that the results are too uncertain to initiate any policy action at the present moment and that an assessment supported by measurements would be warranted.Open Access funding provided by Inland Norway University Of Applied Sciences. It is a part of the overall strategy of the development of the WORLD7 model, supported by the SIMRESS grant (funded by the German Federal Ministry for Environment and the German Environmental Protection Agency (FKZ 3712 93 102).Peer Reviewe

The monitoring of Verkhniy Kaban lake by rbcL gene of freshwater organisms using next-generation sequencing

© 2018 BRNSS Publication Hub. All rights reserved. Aim: The lake Verkhniy Kaban, along with the lakes Sredny Kaban and Nizhny Kaban, makes the part of the Kaban lake system. The lakes are located in the center of the large industrial city and experience anthropogenic load. According to the estimates of the Lake Kaban ecologists, they are related to polluted lakes. Materials and Methods: The sequencing of rbcL gene fragment sequences of the freshwater lake Verkhniy Kaban hydrobionts during autumn (2016) and summer (2017) sampling periods in FASTQ format is included in the international database on NCBI site with the following unique numbers: SRR7470846, SRR7459785, and SRR7463965. Results: The results of the analysis are given, and the water quality evaluation of the Verkhniy Kaban Lake (Kazan, Russia) is presented in the work on the basis of the rbcL gene of freshwater organisms by the method of new generation sequencing. The comparative analysis of metagenomic data shows that the majority of organisms of the Verkhniy Kaban Lake are grouped according to rbcL gene near b-mesosaprobic zone. The Verkhniy Kaban Lake can be characterized as contaminated with the water quality transitional to b-mesosaprobic one. Conclusions: The use of modern methods of molecular biology for the purpose of bioindication gives positive results and increases the effectiveness and reliability of water body ecological state evaluation. The obtained results are of great practical interest in the field of water body monitoring, in particular, and the environment as a whole

Upper ocean manifestations of a reducing meridional overturning circulation

Most climate models predict a slowing down of the Atlantic Meridional Overturning Circulation during the 21st century. Using a 100year climate change integration of a high resolution coupled climate model, we show that a 5.3Sv reduction in the deep southward transport in the subtropical North Atlantic is balanced solely by a weakening of the northward surface western boundary current, and not by an increase in the southward transport integrated across the interior ocean away from the western boundary. This is consistent with Sverdrup balance holding to a good approximation outside of the western boundary region on decadal time scales, and may help to spatially constrain past and future change in the overturning circulation. The subtropical gyre weakens by 3.4Sv over the same period due to a weakened wind stress curl. These changes combine to give a net 8.7Sv reduction in upper western boundary transport. © 2012. American Geophysical Union. All Rights Reserved

Spatial and Temporal Scales of Sverdrup Balance

Sverdrup balance underlies much of the theory of ocean circulation and provides a potential tool for describing the interior ocean transport from only the wind stress. Using both a model state estimate and an eddy-permitting coupled climate model, this study assesses to what extent and over what spatial and temporal scales Sverdrup balance describes the meridional transport. The authors find that Sverdrup balance holds to first order in the interior subtropical ocean when considered at spatial scales greater than approximately 5°. Outside the subtropics, in western boundary currents and at short spatial scales, significant departures occur due to failures in both the assumptions that there is a level of no motion at some depth and that the vorticity equation is linear. Despite the ocean transport adjustment occurring on time scales consistent with the basin-crossing times for Rossby waves, as predicted by theory, Sverdrup balance gives a useful measure of the subtropical circulation after only a few years. This is because the interannual transport variability is small compared to the mean transports. The vorticity input to the deep ocean by the interaction between deep currents and topography is found to be very large in both models. These deep transports, however, are separated from upper-layer transports that are in Sverdrup balance when considered over large scales

Using knowledge: the dilemmas of 'bridging research and policy'

The 'knowledge agenda' has become a central part of development discourse. This paper addresses one aspect of this discourse - the use of policy research in the social sciences - and the dilemmas that have been encountered by both development agencies and researchers in communicating and making use of that research. Development agencies as well as NGOs have initiated work to evaluate and document the effectiveness of research partnerships, knowledge capacity building and (social) science policy impact. As a multilateral initiative, the Global Development Network (GDN), and especially its 'Bridging Research and Policy' project, provides a vehicle to address issues related to research impact. Twelve perspectives on improving research and policy linkages are outlined to reveal that how the problem is defined shapes policy responses. Taken together, these explanations provide a multifaceted picture of the research-policy nexus indicating that there are many possible routes to 'bridging' research and policy. These diverse perspectives will be categorised into three broad categories of explanation: (i) supply-side; (ii) demand-led; and (iii) policy currents. However, knowledge is part of the solution to many development problems but not of itself a panacea

Simulating carbon capture by enhanced weathering with global croplands: an overview of key processes highlighting areas of future model development

Enhanced weathering (EW) aims to amplify a natural sink for CO2 by incorporating powdered silicate rock with high reactive surface area into agricultural soils. The goal is to achieve rapid dissolution of minerals and release of alkalinity with accompanying dissolution of CO2 into soils and drainage waters. EW could counteract phosphorus limitation and greenhouse gas (GHG) emissions in tropical soils, and soil acidification, a common agricultural problem studied with numerical process models over several decades. Here, we review the processes leading to soil acidification in croplands and how the soil weathering CO2 sink is represented in models. Mathematical models capturing the dominant processes and human interventions governing cropland soil chemistry and GHG emissions neglect weathering, while most weathering models neglect agricultural processes. We discuss current approaches to modelling EW and highlight several classes of model having the potential to simulate EW in croplands. Finally, we argue for further integration of process knowledge in mathematical models to capture feedbacks affecting both longer-term CO2 consumption and crop growth and yields

A repurposing strategy for Hsp90 inhibitors demonstrates their potency against filarial nematodes

Novel drugs are required for the elimination of infections caused by filarial worms, as most commonly used drugs largely target the microfilariae or first stage larvae of these infections. Previous studies, conducted in vitro, have shown that inhibition of Hsp90 kills adult Brugia pahangi. As numerous small molecule inhibitors of Hsp90 have been developed for use in cancer chemotherapy, we tested the activity of several novel Hsp90 inhibitors in a fluorescence polarization assay and against microfilariae and adult worms of Brugia in vitro. The results from all three assays correlated reasonably well and one particular compound, NVP-AUY922, was shown to be particularly active, inhibiting Mf output from female worms at concentrations as low as 5.0 nanomolar after 6 days exposure to drug. NVP-AUY922 was also active on adult worms after a short 24 h exposure to drug. Based on these in vitro data, NVP-AUY922 was tested in vivo in a mouse model and was shown to significantly reduce the recovery of both adult worms and microfilariae. These studies provide proof of principle that the repurposing of currently available Hsp90 inhibitors may have potential for the development of novel agents with macrofilaricidal properties

Laboratory experiments on eddy generation by a buoyant coastal current flowing over variable bathymetry

Author Posting. © American Meteorological Society, 2006. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 36 (2006): 395-411, doi:10.1175/JPO2857.1.Irminger rings are warm-core eddies formed off the west coast of Greenland. Recent studies suggest that these eddies, which are implicated in the rapid springtime restratification of the Labrador Sea, are formed by an internal instability of the West Greenland Current (WGC), triggered by bathymetric variations. This study seeks to explore the effect of the magnitude and downstream length scale of bathymetric variations on the stability of a simple model of the WGC in a series of laboratory experiments in which a buoyant coastal current was allowed to flow over bathymetry consisting of piecewise constant slopes of varying magnitude. The currents did not form eddies over gently sloping bathymetry and only formed eddies over steep bathymetry if the current width exceeded the width of the sloping bathymetry. Eddying currents were immediately stabilized if they flowed onto gently sloping topography. Bathymetric variations that persisted only a short distance downstream perturbed the flow locally but did not lead to eddy formation. Eddies formed only once the downstream length of the bathymetric variations exceeded a critical scale of about 8 Rossby radii. These results are consistent with the observed behavior of the WGC, which begins to form Irminger rings after entering a region where the continental slope abruptly steepens and becomes narrower than the WGC itself in a region spanning about 20–80 Rossby radii of downstream distance.The authors gratefully acknowledge the National Science Foundation (Grant OCE- 9810657) and the Office of Naval Research (Grant N00014-97-1-0934) for their support of the 2003 WHOI Geophysical Fluid Dynamics Summer School where much of the research presented in this paper was performed. CLW received additional support from the Office of Naval Research Grant N00014-98-1-0813

Quantitative localized proton-promoted dissolution kinetics of calcite using scanning electrochemical microscopy (SECM)

Scanning electrochemical microscopy (SECM) has been used to determine quantitatively the kinetics of proton-promoted dissolution of the calcite (101̅4) cleavage surface (from natural “Iceland Spar”) at the microscopic scale. By working under conditions where the probe size is much less than the characteristic dislocation spacing (as revealed from etching), it has been possible to measure kinetics mainly in regions of the surface which are free from dislocations, for the first time. To clearly reveal the locations of measurements, studies focused on cleaved “mirror” surfaces, where one of the two faces produced by cleavage was etched freely to reveal defects intersecting the surface, while the other (mirror) face was etched locally (and quantitatively) using SECM to generate high proton fluxes with a 25 μm diameter Pt disk ultramicroelectrode (UME) positioned at a defined (known) distance from a crystal surface. The etch pits formed at various etch times were measured using white light interferometry to ascertain pit dimensions. To determine quantitative dissolution kinetics, a moving boundary finite element model was formulated in which experimental time-dependent pit expansion data formed the input for simulations, from which solution and interfacial concentrations of key chemical species, and interfacial fluxes, could then be determined and visualized. This novel analysis allowed the rate constant for proton attack on calcite, and the order of the reaction with respect to the interfacial proton concentration, to be determined unambiguously. The process was found to be first order in terms of interfacial proton concentration with a rate constant k = 6.3 (± 1.3) × 10–4 m s–1. Significantly, this value is similar to previous macroscopic rate measurements of calcite dissolution which averaged over large areas and many dislocation sites, and where such sites provided a continuous source of steps for dissolution. Since the local measurements reported herein are mainly made in regions without dislocations, this study demonstrates that dislocations and steps that arise from such sites are not needed for fast proton-promoted calcite dissolution. Other sites, such as point defects, which are naturally abundant in calcite, are likely to be key reaction sites