The polarized expression of Na+,K+-ATPase in epithelia depends on the association between beta-subunits located in neighboring cells

The polarized distribution of Na+,K+-ATPase plays a paramount physiological role, because either directly or through coupling with co- and countertransporters, it is responsible for the net movement of, for example, glucose, amino acids, Ca2+, K+, Cl-, and CO3H- across the whole epithelium. We report here that the beta-subunit is a key factor in the polarized distribution of this enzyme. 1) Madin-Darby canine kidney (MDCK) cells (epithelial from dog kidney) express the Na+,K+-ATPase over the lateral side, but not on the basal and apical domains, as if the contact with a neighboring cell were crucial for the specific membrane location of this enzyme. 2) MDCK cells cocultured with other epithelial types (derived from human, cat, dog, pig, monkey, rabbit, mouse, hamster, and rat) express the enzyme in all (100%) homotypic MDCK/MDCK borders but rarely in heterotypic ones. 3) Although MDCK cells never express Na+,K+-ATPase at contacts with Chinese hamster ovary (CHO) cells, they do when CHO cells are transfected with beta(1)-subunit from the dog kidney (CHO-beta). 4) This may be attributed to the adhesive property of the beta(1)-subunit, because an aggregation assay using CHO (mock-transfected) and CHO-beta cells shows that the expression of dog beta(1)-subunit in the plasma membrane does increase adhesiveness. 5) This adhesiveness does not involve adherens or tight junctions. 6) Transfection of beta(1)-subunit forces CHO-beta cells to coexpress endogenous a-subunit. Together, our results indicate that MDCK cells express Na+,K+-ATPase at a given border provided the contacting cell expresses the dog P,-subunit. The cell-cell interaction thus established would suffice to account for the polarized expression and positioning of Na+,K+-ATPase in epithelial cells

Effects of Hypohydration on Work Performance and Tolerance to plus Gz Acceleration in Man

Hypohydration effects on work performance and tolerance to acceleration stress in ma

Stretch-Induced Hypertrophy Activates NFkB-Mediated VEGF Secretion in Adult Cardiomyocytes

Hypertension and myocardial infarction are associated with the onset of hypertrophy. Hypertrophy is a compensatory response mechanism to increases in mechanical load due to pressure or volume overload. It is characterized by extracellular matrix remodeling and hypertrophic growth of adult cardiomyocytes. Production of Vascular Endothelial Growth Factor (VEGF), which acts as an angiogenic factor and a modulator of cardiomyocyte function, is regulated by mechanical stretch. Mechanical stretch promotes VEGF secretion in neonatal cardiomyocytes. Whether this effect is retained in adult cells and the molecular mechanism mediating stretch-induced VEGF secretion has not been elucidated. Our objective was to investigate whether cyclic mechanical stretch induces VEGF secretion in adult cardiomyocytes and to identify the molecular mechanism mediating VEGF secretion in these cells. Isolated primary adult rat cardiomyocytes (ARCMs) were subjected to cyclic mechanical stretch at an extension level of 10% at 30 cycles/min that induces hypertrophic responses. Cyclic mechanical stretch induced a 3-fold increase in VEGF secretion in ARCMs compared to non-stretch controls. This increase in stretch-induced VEGF secretion correlated with NFkB activation. Cyclic mechanical stretch-mediated VEGF secretion was blocked by an NFkB peptide inhibitor and expression of a dominant negative mutant IkBα, but not by inhibitors of the MAPK/ERK1/2 or PI3K pathways. Chromatin immunoprecipitation assays demonstrated an interaction of NFkB with the VEGF promoter in stretched primary cardiomyocytes. Moreover, VEGF secretion is increased in the stretched myocardium during pressure overload-induced hypertrophy. These findings are the first to demonstrate that NFkB activation plays a role in mediating VEGF secretion upon cyclic mechanical stretch in adult cardiomyocytes. Signaling by NFkB initiated in response to cyclic mechanical stretch may therefore coordinate the hypertrophic response in adult cardiomyocytes. Elucidation of this novel mechanism may provide a target for developing future pharmacotherapy to treat hypertension and heart disease

Inner disk clearing around the Herbig Ae star HD\,139614: Evidence for a planet-induced gap ?

Spatially resolving the inner dust cavity of the transitional disks is a key to understanding the connection between planetary formation and disk dispersal. The disk around the Herbig star HD 139614 is of particular interest since it presents a pretransitional nature with an au-sized gap, in the dust, that was spatially resolved by mid-IR interferometry. Using new NIR interferometric observations, we aim to characterize the 0.1-10~au region of the HD~139614 disk further and identify viable mechanisms for the inner disk clearing. We report the first multiwavelength radiative transfer modeling of the interferometric data acquired on HD~139614 with PIONIER, AMBER, and MIDI, complemented by Herschel/PACS photometries. We confirm a gap structure in the um-sized dust, extending from about 2.5 au to 6 au, and constrained the properties of the inner dust component: e.g., a radially increasing surface density profile, and a depletion of 10^3 relative to the outer disk. Since self-shadowing and photoevaporation appears unlikely to be responsible for the au-sized gap of HD~139614, we thus tested if dynamical clearing could be a viable mechanism using hydrodynamical simulations to predict the gaseous disk structure. Indeed, a narrow au-sized gap is expected when a single giant planet interacts with the disk. Assuming that small dust grains are well coupled to the gas, we found that a ~ 3~Mjup planet located at 4.5 au from the star could, in less than 1 Myr, reproduce most of the aspects of the dust surface density profile, while no significant depletion in gas occurred in the inner disk, in contrast to the dust. However, the dust-depleted inner disk could be explained by the expected dust filtration by the gap and the efficient dust growth/fragmentation in the inner disk regions. Our results support the hypothesis of a giant planet opening a gap and shaping the inner region of the HD~139614 disk.Comment: Version accepted in A&A, with typos corrections in the tex

The 2009 Benedictine Heritage Tour: Overview of the experience

Members of the 2009 Benedictine Heritage Tour who traveled to Italy and Germany in June of 2009 will share their experiences of the trip. This tour was the first of its kind, but there will be two tours in 2010 (one in May and one in June, as recently announced). The purpose of the tour is to allow staff and faculty to explore our Benedictine heritage. The tour includes visits to important sites in Rome, Subiaco and Monte Cassino (the two monasteries founded by St. Benedict), Munich, and Eichstatt and Metten (the monasteries from which the founding nuns and monks of CSB/SJU came). Employees (staff and faculty) who are considering applying for one of the 2010 tours are especially encouraged to attend. The twelve 2009 participants are members of the monastic community, administrative staff, support staff, and faculty. We had an incredibly awesome trip

Effects of acute and chronic hypohydration on tolerance to plus Gz acceleration in man. I - Physiological results

Effects of acute and chronic hypohydration on human physiological tolerance to gravity acceleratio

Sigurðar saga fóts (The Saga of Sigurðr Foot): A Translation

This is the first English translation of the short Icelandic romance Sigurðar saga fóts, with an introduction presenting the evidence for its dating and immediate literary context. Like most Icelandic romances, Sigurðar saga is a bridal-quest story; the support of a foster-brother is key to the hero winning the bride; and the foster-brothers start out as opponents before recognising their mutual excellence and swearing foster-brotherhood. Uniquely, however, the men who become foster-brothers begin by competing for the same bride (Signý): the eponymous Sigurðr fótr wins Signý only because Ásmundr gives her to him in exchange for foster-brotherhood. Ásmundr’s decision can be read as demonstrating with unusual starkness the superior importance in much Icelandic romance of homosocial relationships over heterosexual ones, giving the saga a certain paradigmatic status. Translating the saga in an open-access forum and reconstructing its literary context will, we hope, encourage further analyses