Making and Breaking Trust in Forest Collaborative Groups

There has been a recent increase in use of an organized, forest ‘collaborative’ group approach for multi-stakeholder input on federal forestlands in the U.S. West. This approach relies on the creation of shared trust to achieve social agreement. Yet growing critiques suggest a lack of trust in the U.S. Forest Service [Forest Service], between stakeholders, and the collaborative process itself. We conducted three comparative case studies of established forest collaborative groups in Oregon, Washington, and Idaho to ask how trust is created and damaged or broken in this context. We found multiple, interlinked dimensions to trust, including significant reliance on procedural trust, trust of ‘in-groups’ who shared norms for conduct, and distrust of new participants. We also found that trust or distrust in the Forest Service affected other trust and process dynamics within groups. Our research offers new insights into the functions and limitations of a collaborative approach that is increasingly central to federal forest governance; and new empirical knowledge toward recent theoretical developments about trust in natural resource collaboration

Oxylipins at intermediate larval stages of damselfly Coenagrion hastulatum as biochemical biomarkers for anthropogenic pollution

Aquatic pollution resulting from anthropogenic activities requires adequate environmental monitoring strategies in sentinel organisms. Thus, biochemical biomarkers have been used as early-warning tools of biological effects in aquatic organisms. However, before using these markers for environmental monitoring, knowledge about their developmental variation is vital. In this study, we assessed baseline levels and developmental variations of a group of potential biomarkers, oxylipins, during the lifespan of the Northern damselfly (Coenagrion hastulatum) using liquid chromatography-tandem mass spectrometry. Effects of wastewater exposure on baseline levels were studied in a subset of damselflies to investigate the responsiveness due to anthropogenic pollution. Thirty-eight oxylipins deriving from four polyunsaturated fatty acids via two enzymatic pathways were detected in damselflies at three larval stages and in the adult form. Overall, oxylipin baseline levels showed developmental variation, which was lowest in the intermediate larval stages. Effects of exposure to wastewater effluent on oxylipin baseline levels were dependent on the life stage and were greatest in the early and intermediate larval stages. The study provides first insights into oxylipin profiles of damselflies at different stages of development and their developmental variation. Based on our results, we propose further strategies for incorporating oxylipins in damselfly larvae as biochemical markers for anthropogenic pollution

New WMO certified megaflash lightning extremes for flash distance (768 km) and duration (17.01 seconds) recorded from space

Initial global extremes in lightning duration and horizontal distance were established in 2017 (Lang et al. 2017) by an international panel of atmospheric lightning scientists and engineers assembled by the WMO. The subsequent launch of NOAA’s latest GOES-16/17 satellites with their Geostationary Lightning Mappers (GLMs) enabled extreme lightning to be monitored continuously over the western hemisphere up to 55° latitude for the first time. As a result, the former lightning extremes were more than doubled in 2019 to 709 km for distance and 16.730 s for duration (Peterson et al. 2020). Continued detection and analysis of lightning “megaflashes” (Sequin, 2021) has now revealed two flashes that even exceed those 2019 records. As part of the ongoing work of the WMO in detection and documentation of global weather extremes (e.g., El Fadli et al. 2013; Merlone et al. 2010), an international WMO evaluation committee was created to critically adjudicate these two GLM megaflash cases as new records for extreme lightning.We thank S. A. Rutledge and two other reviewers for their valuable comments. M. J. Peterson was supported by the U.S. Department of Energy through the Los Alamos National Laboratory (LANL) Laboratory Directed Research and Development (LDRD) program under project number 20200529ECR. Los Alamos National Laboratory is operated by Triad National Security, LLC, for the National Nuclear Security Administration of U.S. Department of Energy (Contract 89233218CNA000001). T. Logan supported by a NOAA Grant NA16OAR4320115 “Lightning Mapper Array Operation in Oklahoma and the Texas Gulf Coast Region to Aid Preparation for the GOES-R GLM.” I. Kolmasova was supported by GACR Grant 20-09671. S. D. Zhang was supported by a NOAA Grant NNH19ZDA001N-ESROGSS. The participation of J. Montanya in this work is supported by research Grant ESP2017-86263-C4-2-R funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe,” by the “European Union”; and Grants PID2019-109269RB-C42 funded by MCIN/AEI/10.13039/501100011033.Peer ReviewedPostprint (author's final draft

Molecular architecture of a dynamin adaptor: implications for assembly of mitochondrial fission complexes

Structural and functional studies of the Mdv1 dimer reveal how this mitochondria-associated adaptor protein orients and stabilizes the assembly of dynamins on membranes to promote mitochondrial fission

WMO Assessment of Weather and Climate Mortality Extremes: Lightning, Tropical Cyclones, Tornadoes, and Hail

A World Meteorological Organization (WMO) Commission for Climatology international panel was convened to examine and assess the available evidence associated with five weather-related mortality extremes: 1) lightning (indirect), 2) lightning (direct), 3) tropical cyclones, 4) tornadoes, and 5) hail. After recommending for acceptance of only events after 1873 (the formation of the predecessor of the WMO), the committee evaluated and accepted the following mortality extremes: 1) “highest mortality (indirect strike) associated with lightning” as the 469 people killed in a lightning-caused oil tank fire in Dronka, Egypt, on 2 November 1994; 2) “highest mortality directly associated with a single lightning flash” as the lightning flash that killed 21 people in a hut in Manica Tribal Trust Lands, Zimbabwe (at time of incident, eastern Rhodesia), on 23 December 1975; 3) “highest mortality associated with a tropical cyclone” as the Bangladesh (at time of incident, East Pakistan) cyclone of 12–13 November 1970 with an estimated death toll of 300 000 people; 4) “highest mortality associated with a tornado” as the 26 April 1989 tornado that destroyed the Manikganj district, Bangladesh, with an estimated death toll of 1300 individuals; and 5) “highest mortality associated with a hailstorm” as the storm occurring near Moradabad, India, on 30 April 1888 that killed 246 people. These mortality extremes serve to further atmospheric science by giving baseline mortality values for comparison to future weather-related catastrophes and also allow for adjudication of new meteorological information as it becomes available

Rpgrip1 is required for rod outer segment development and ciliary protein trafficking in zebrafish

The authors would like to thank the Royal Society of London, the National Eye Research Centre, the Visual Research Trust, Fight for Sight, the W.H. Ross Foundation, the Rosetrees Trust, and the Glasgow Children’s Hospital Charity for supporting this work. This work was also supported by the Deanship of Scientific Research at King Saud University for funding this research (Research Project) grant number ‘RGP – VPP – 219’.Mutations in the RPGR-interacting protein 1 (RPGRIP1) gene cause recessive Leber congenital amaurosis (LCA), juvenile retinitis pigmentosa (RP) and cone-rod dystrophy. RPGRIP1 interacts with other retinal disease-causing proteins and has been proposed to have a role in ciliary protein transport; however, its function remains elusive. Here, we describe a new zebrafish model carrying a nonsense mutation in the rpgrip1 gene. Rpgrip1homozygous mutants do not form rod outer segments and display mislocalization of rhodopsin, suggesting a role for RPGRIP1 in rhodopsin-bearing vesicle trafficking. Furthermore, Rab8, the key regulator of rhodopsin ciliary trafficking, was mislocalized in photoreceptor cells of rpgrip1 mutants. The degeneration of rod cells is early onset, followed by the death of cone cells. These phenotypes are similar to that observed in LCA and juvenile RP patients. Our data indicate RPGRIP1 is necessary for rod outer segment development through regulating ciliary protein trafficking. The rpgrip1 mutant zebrafish may provide a platform for developing therapeutic treatments for RP patients.Publisher PDFPeer reviewe

Abrogation of Stem Loop Binding Protein (Slbp) function leads to a failure of cells to transition from proliferation to differentiation, retinal coloboma and midline axon guidance deficits

Through forward genetic screening for mutations affecting visual system development, we identified prominent coloboma and cell-autonomous retinal neuron differentiation, lamination and retinal axon projection defects in eisspalte (ele) mutant zebrafish. Additional axonal deficits were present, most notably at midline axon commissures. Genetic mapping and cloning of the ele mutation showed that the affected gene is slbp, which encodes a conserved RNA stem-loop binding protein involved in replication dependent histone mRNA metabolism. Cells throughout the central nervous system remained in the cell cycle in ele mutant embryos at stages when, and locations where, post-mitotic cells have differentiated in wild-type siblings. Indeed, RNAseq analysis showed down-regulation of many genes associated with neuronal differentiation. This was coincident with changes in the levels and spatial localisation of expression of various genes implicated, for instance, in axon guidance, that likely underlie specific ele phenotypes. These results suggest that many of the cell and tissue specific phenotypes in ele mutant embryos are secondary to altered expression of modules of developmental regulatory genes that characterise, or promote transitions in, cell state and require the correct function of Slbp-dependent histone and chromatin regulatory genes