34 research outputs found
The Ventian mammal age (Lastest Miocene): present state
La edad de mamíferos terrestres Ventiense incluye la mayoría de las faunas españolas de la unidad biocronológica MN 13. Se correlaciona con el Messiniense, aunque su parte fi nal puede incluir faunas de edad Plioceno basal. El Ventiense comienza con la primera aparición del genero de Muridae Stephanomys (7 Ma; datación paleomagnética de El Bunker, cuenca de Teruel), y termina con la aparición de Promimomys (ca. 5 Ma), ambas registradas en la cuenca de Teruel. En este trabajo proponemos una nueva organización del Ventiense. Una primera división correspondiente a la zona M (Dam et al., 2001), y una nueva zona denominada N, equivalente a la zona con Celadensia (Mein et al., 1990; Dam et al., 2001) más la parte de la zona con dos Paraethomys (Dam et al., 2006) en la que Celadensia ha desaparecido y aún no se registra Promimomys. El Ventiense es ahora reconocido con límites y divisiones precisas, de forma que puede ser fácilmente reconocible en las cuencas continentales Ibéricas con sedimentos Mio-Pliocenos permitiendo unas correlaciones intra e inter cuenca detalladas
Prognostic significance of FLT3-ITD length in AML patients treated with intensive regimens
FLT3-ITD mutations are detected in approximately 25% of newly diagnosed adult acute myeloid leukemia (AML) patients and confer an adverse prognosis. The FLT3-ITD allelic ratio has clear prognostic value. Nevertheless, there are numerous manuscripts with contradictory results regarding the prognostic relevance of the length and insertion site (IS) of the FLT3-ITD fragment. We aimed to assess the prognostic impact of these variables on the complete remission (CR) rates, overall survival (OS) and relapse-free survival (RFS) of AML patients with FLT3-ITDmutations. We studied the FLT3-ITD length of 362 adult AML patients included in the PETHEMA AML registry. We tried to validate the thresholds of ITD length previously published (i.e., 39 bp and 70 bp) in intensively treated AML patients (n = 161). We also analyzed the mutational profile of 118 FLT3-ITD AML patients with an NGS panel of 39 genes and correlated mutational status with the length and IS of ITD. The AUC of the ROC curve of the ITD length for OS prediction was 0.504, and no differences were found when applying any of the thresholds for OS, RFS or CR rate. Only four out of 106 patients had ITD IS in the TKD1 domain. Our results, alongside previous publications, confirm that FLT3-ITD length lacks prognostic value and clinical applicability. © 2021, The Author(s)
Genetic landscape of 6089 inherited retinal dystrophies affected cases in Spain and their therapeutic and extended epidemiological implications
Inherited retinal diseases (IRDs), defined by dysfunction or progressive loss of photoreceptors, are disorders characterized by elevated heterogeneity, both at the clinical and genetic levels. Our main goal was to address the genetic landscape of IRD in the largest cohort of Spanish patients reported to date. A retrospective hospital-based cross-sectional study was carried out on 6089 IRD affected individuals (from 4403 unrelated families), referred for genetic testing from all the Spanish autonomous communities. Clinical, demographic and familiar data were collected from each patient, including family pedigree, age of appearance of visual symptoms, presence of any systemic findings and geographical origin. Genetic studies were performed to the 3951 families with available DNA using different molecular techniques. Overall, 53.2% (2100/3951) of the studied families were genetically characterized, and 1549 different likely causative variants in 142 genes were identified. The most common phenotype encountered is retinitis pigmentosa (RP) (55.6% of families, 2447/4403). The most recurrently mutated genes were PRPH2, ABCA4 and RS1 in autosomal dominant (AD), autosomal recessive (AR) and X-linked (XL) NON-RP cases, respectively; RHO, USH2A and RPGR in AD, AR and XL for non-syndromic RP; and USH2A and MYO7A in syndromic IRD. Pathogenic variants c.3386G > T (p.Arg1129Leu) in ABCA4 and c.2276G > T (p.Cys759Phe) in USH2A were the most frequent variants identified. Our study provides the general landscape for IRD in Spain, reporting the largest cohort ever presented. Our results have important implications for genetic diagnosis, counselling and new therapeutic strategies to both the Spanish population and other related populations.This work was supported by the Instituto de Salud Carlos III (ISCIII) of the Spanish Ministry of Health (FIS; PI16/00425 and PI19/00321), Centro de Investigación Biomédica en Red Enfermedades Raras (CIBERER, 06/07/0036), IIS-FJD BioBank (PT13/0010/0012), Comunidad de Madrid (CAM, RAREGenomics Project, B2017/BMD-3721), European Regional Development Fund (FEDER), the Organización Nacional de Ciegos Españoles (ONCE), Fundación Ramón Areces, Fundación Conchita Rábago and the University Chair UAM-IIS-FJD of Genomic Medicine. Irene Perea-Romero is supported by a PhD fellowship from the predoctoral Program from ISCIII (FI17/00192). Ionut F. Iancu is supported by a grant from the Comunidad de Madrid (CAM, PEJ-2017-AI/BMD7256). Marta del Pozo-Valero is supported by a PhD grant from the Fundación Conchita Rábago. Berta Almoguera is supported by a Juan Rodes program from ISCIII (JR17/00020). Pablo Minguez is supported by a Miguel Servet program from ISCIII (CP16/00116). Marta Corton is supported by a Miguel Servet program from ISCIII (CPII17/00006). The funders played no role in study design, data collection, data analysis, manuscript preparation and/or publication decisions
Effects of diacerein on cartilage and subchondral bone in early stages of osteoarthritis in a rabbit model
Comparison of various SYSADOA for the osteoarthritis treatment: an experimental study in rabbits
Influence of the para-aminohippuric acid analysis method on the net hepatic flux of nutrients in lactating cows1
International audiencePara-aminohippuric acid (pAH) is a marker frequently used to measure plasma or blood flow. In sheep studies, it is recognized that its determination must include a deacetylation step to counteract the hepatic acetylation of pAH. Such a procedure is not of common usage in cattle studies although a recent suggestion of hepatic pAH acetylation in dairy cows may have important consequences for hepatic nutrient fluxes. The aims of this study were to evaluate pAH analytical methods according to international procedures of metrology and confirm hepatic acetylation of pAH in dairy cows. The effect of the matrix used to prepare the standard curve was tested, and the influence of the pAH analytical method on blood flows and subsequent net hepatic fluxes of nutrients was determined. For the first objective, accuracy profiles were established and bias, indicators of precision, and limits of quantification were reported for 2 analytical methods (without and with a pAH deacetylation step) using 2 different standard matrices (water and plasma). Second, the net hepatic flux of different nutrients was determined including or not the deacetylation step and preparing the standard curves in plasma using samples collected from 5 multicatheterized lactating Jersey cows. The choice of the matrix had a significant impact on plasma pAH concentrations as illustrated by accuracy profiles. Water matrix decreased (P < 0.01) the slope, y-intercept, and the absorbance at concentration 0 mg pAH/L of the standard curve in both methods (without and with the deacetylation), supporting that standards prepared in water should not be used to analyze plasma samples. Samples collected on cows confirmed hepatic acetylation of pAH across the liver. Deacetylation, performed using plasma as the standard matrix, increased (P < 0.05) plasma pAH concentrations from 18.4, 26, and 23.5 to 21.4, 28.9, and 27.3 mg/L in the artery, portal vein, and hepatic vein, respectively. Deacetylation decreased the hepatic venous and arterial plasma and blood flows (P < 0.05) by 9 and 55%, respectively, modifying the net hepatic flux of acetate, total amino acid, and oxygen by more than 19% (P < 0.05). In conclusion, our results highlight the importance of including a deacetylation step in the pAH analysis method in cattle studies and of using plasma as standard matri
Integrated Management of 'Bemisia' and other Tomato Pests in Commerical Greenhouses of Spain
Really does temperature reduction and norepinephrine have similar effects on the energy metabolism in rat brown adipose tissue?
The Ventian mammal age (Lastest Miocene): present state
La edad de mamíferos terrestres Ventiense incluye la mayoría de las faunas españolas de la unidad biocronológica MN 13. Se correlaciona con el Messiniense, aunque su parte fi nal puede incluir faunas de edad Plioceno basal. El Ventiense comienza con la primera aparición del genero de Muridae Stephanomys (7 Ma; datación paleomagnética de El Bunker, cuenca de Teruel), y termina con la aparición de Promimomys (ca. 5 Ma), ambas registradas en la cuenca de Teruel. En este trabajo proponemos una nueva organización del Ventiense. Una primera división correspondiente a la zona M (Dam et al., 2001), y una nueva zona denominada N, equivalente a la zona con Celadensia (Mein et al., 1990; Dam et al., 2001) más la parte de la zona con dos Paraethomys (Dam et al., 2006) en la que Celadensia ha desaparecido y aún no se registra Promimomys. El Ventiense es ahora reconocido con límites y divisiones precisas, de forma que puede ser fácilmente reconocible en las cuencas continentales Ibéricas con sedimentos Mio-Pliocenos permitiendo unas correlaciones intra e inter cuenca detalladas