Four new coordination polymers involving transition metals with 1,2,4,5-benzenetetracarboxylate and pyridyl-donor ligand di(4-pyridyl) sulfide

AbstractFour new coordination polymers namely {[Mn2(BT)(DPS)2(H2O)6]·10H2O}n (MnBTDPS), {[Co2(BT)(DPS)2(H2O)6]·10H2O}n (CoBTDPS), {[Cu2(BT)(DPS)(H2O)4]·5H2O}n (CuBTDPS) and {[Zn2(BT)(DPS)2]·6H2O}n (ZnBTDPS), where BT=1,2,4,5-benzenetetracarboxylate and DPS=di(4-pyridyl) sulfide, were synthesized and characterized by thermal analysis, vibrational spectroscopy (Raman and infrared) and single crystal X-ray diffraction analysis. In all compounds, the DPS ligands are coordinated to metal sites in a bridging mode and the carboxylate moiety of BT ligands adopts a monodentate coordination mode, as indicated by the Raman spectra data through the Δν (νasym(COO)−ν sym(COO)) value. According to X-ray diffraction analysis, MnBTDPS and CoBTDPS are isostructural and in these cases, the metal centers exhibit a distorted octahedral geometry. In CuBTBPP, the Cu2+ centers geometries are best described as square-pyramids, according to the trigonality index τ=0.14 for Cu1 and τ=0.10 for Cu2. On the other hand, in ZnBTDPS, the Zn2+ sites adopt a tetrahedral geometry. Finally, the four compounds formed two-dimensional sheets that are connected to each other through hydrogen bonding giving rise to three-dimensional supramolecular arrays

Differences and similarities of postprandial lipemia in rodents and humans

The rat has been a mainstay of physiological and metabolic research, and more recently mice. This study aimed at characterizing the postprandial triglyceride profile of two members of the Muridae family: the Wistar rats (Rattus norvegicus albinus) and C57BL/6 mice (Mus musculus) plus comparing them to the profile obtained in humans. Thirty-one male and twelve female Wistar rats, ten C57BL/6 male and nine female mice received a liquid meal containing fat (17%), protein (4%) and carbohydrates (4%), providing 2 g fat/Kg. Thirty-one men and twenty-nine women received a standardized liquid meal containing fat (25%), dextromaltose (55%), protein (14%), and vitamins and minerals (6%), and providing 40 g of fat per square meter of body surface. Serial blood samples were collected at 2, 4, 6, 8 and 10 h after the ingestion in rats, at 1, 2, 3, 4, 5 and 6 h in mice and in humans at 2, 4, 6 and 8 h. Wilcoxon and Mann-Whitney tests were used. The triglyceride responses were evaluated after the oral fat loads. Fasting and postprandial triglyceridemia were determined sequentially in blood sample. AUC, AUIC, AR, RR and late peaks were determined. Rats are prone to respond in a pro-atherogenic manner. The responses in mice were closer to the ones in healthy men. This study presents striking differences in postprandial triglycerides patterns between rats and mice not correlated to baseline triglycerides, the animal baseline body weight or fat load in all animal groups.101CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPNão temNão te

Macaúba (Acrocomia aculeata) cake from biodiesel processing: a low-cost substrate to produce lipases from Moniliella spathulata R25L270 with potential application in the oleochemical industry

[Background]: Biodiesel industry wastes were evaluated as supplements for lipase production by Moniliella spathulata R25L270, which is newly identified yeast with great lipolytic potential. Macaúba cake (MC), used for the first time in this work as inducer to produce lipases, and residual oil (RO) were mixed to maximise enzyme production. The lipase secreted was biochemically characterised.[Results]: The best ratio for the mixture (MC:RO) was 0.66:0.34 and the fitted values for lipase activity and total protein concentration were 0.98 U mL−1 and 0.356 mg mL−1, respectively. Maximum activity obtained (2.47 U mL−1) was achieved at 31.5°C and pH 6.7, and the enzyme was stable in this condition. A novel enzyme was purified and identified for the first time by mass spectrometry. The lipase efficiently hydrolysed different natural oils and exhibited selectivity in the production of eicosapentaenoic acid from fish oil.[Conclusion]: The use of MC and RO as a supplement to produce the new lipase from M. spathulata R25L270 may be one alternative for reducing lipase production costs and simultaneously adding value to biodiesel industry residues. The potential application of the lipase in the oleochemical industry was demonstrated by its pH and temperature stabilities and selective hydrolysis.This research was supported by Brazilian agencies: CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), INCT (Instituto Nacional de Ciência e Tecnologia) de Nanomateriais de Carbono, FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais), Rede Mineira de Toxinas com Ação Terapêutica and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior).Peer reviewe

ST-Elevation myocardial infarction network: systematization in 205 cases reduced clinical events in the public health care system

BACKGROUND: The major cause of death in the city of São Paulo (SP) is cardiac events. At its periphery, in-hospital mortality in acute myocardial infarction is estimated to range between 15% and 20% due to difficulties inherent in large metropoles. OBJECTIVE:To describe in-hospital mortality in ST-segment elevation acute myocardial infarction (STEMI) of patients admitted via ambulance or peripheral hospitals, which are part of a structured training network (STEMI Network). METHODS: Health care teams of four emergency services (Ermelino Matarazzo, Campo Limpo, Tatuapé and Saboya) of the periphery of the city of São Paulo and advanced ambulances of the Emergency Mobile Health Care Service (abbreviation in Portuguese, SAMU) were trained to use tenecteplase or to refer for primary angioplasty. A central office for electrocardiogram reading was used. After thrombolysis, the patient was sent to a tertiary reference hospital to undergo cardiac catheterization immediately (in case of failed thrombolysis) or in 6 to 24 hours, if the patient was stable. Quantitative and qualitative variables were assessed by use of uni- and multivariate analysis. RESULTS: From January 2010 to June 2011, 205 consecutive patients used the STEMI Network, and the findings were as follows: 87 anterior wall infarctions; 11 left bundle-branch blocks; 14 complete atrioventricular blocks; and 14 resuscitations after initial cardiorespiratory arrest. In-hospital mortality was 6.8% (14 patients), most of which due to cardiogenic shock, one hemorrhagic cerebrovascular accident, and one bleeding. CONCLUSION: The organization in the public health care system of a network for the treatment of STEMI, involving diagnosis, reperfusion, immediate transfer, and tertiary reference hospital, resulted in immediate improvement of STEMI outcomes.FUNDAMENTO: A principal causa de óbitos na cidade de São Paulo (SP) é por eventos cardíacos. Em hospitais periféricos de São Paulo estima-se a mortalidade hospitalar no infarto agudo entre 15% e 20%, pelas dificuldades existentes. OBJETIVO: Descrever a mortalidade intra-hospitalar do Infarto Agudo do Miocárdio com Supradesnivelamento de ST (IAMCSST) de pacientes admitidos via ambulância ou hospitais periféricos, como resultado da organização de uma estruturada rede de treinamento. MÉTODOS: Equipes de quatro prontos-socorros (Ermelino Matarazzo, Campo Limpo, Tatuapé e Saboya) e das ambulâncias avançadas do Serviço de Atendimento Móvel de Urgência (Samu) foram treinadas para uso de tenecteplase (TNK) ou para encaminhamento para angioplastia primária. Uma central de leitura de eletrocardiogramas foi usada quando necessário. Após uso de trombolítico, um hospital terciário recebia o paciente que era submetido a cinecoronariografia imediata (trombólise sem sucesso) ou entre 6 e 24 h, caso estável. Variáveis quantitativas, qualitativas foram avaliadas em análise uni e multivariável. RESULTADOS: De janeiro 2010 a junho 2011, 205 pacientes consecutivos utilizaram a rede de atendimento, ocorrendo 87 infartos de parede anterior, 11 bloqueios de ramo esquerdo, 14 bloqueios atrioventricular total, e em 14 houve reanimação pós-parada cardiorrespiratória inicial. A mortalidade intra-hospitalar foi de 6,8% (14 casos), a maioria por choque cardiogênico, um por acidente vascular encefálico hemorrágico e um por sangramento. CONCLUSÃO: A organização em instituições públicas de uma rede de tratamento, envolvendo diagnóstico, reperfusão, transporte imediato e hospital de retaguarda resultou em melhora imediata dos resultados de IAMCSST.Universidade Federal de São Paulo (UNIFESP), Escola Paulista de Medicina (EPM)Serviço de Atendimento Móvel de UrgênciaPrefeitura Municipal de São Paulo Secretaria de SaúdeHospital Municipal Prof. Dr. Alípio Correa NettoUNIFESP, EPMSciEL

Evaluation of hybrid-short-coir-fibre-reinforced composites via full factorial design

A full factorial design (2231) has been used to investigate the effect of the useof sodium hydroxide fibre treatment, Portland cement and uniaxial pressure on the physical and mechanical properties of hybrid short coir fibre reinforced composites (HSCoirFRCs). The response variables considered in this work were the apparent density, porosity, tensile and flexural strength, the modulus of elasticity and the Charpy impact resistance. The alkali treatment contributed not only to reduce the apparent porosity, but also to increase the mechanical properties of the HSCoirFRCs. A reduction of the impact resistance and an increase of the apparent density was also identified after treatment. Cold pressing significantly affected the physical and mechanical properties of the HSCoirFRCs. Higher pressure levels enhanced the wettability of the fibres and, consequently, the mechanical performance of the composites. The incorporation of cement microparticles as a second reinforcement phase was however not effective, leading to decreased strength and an increased apparent density of the materials. The HSCoirFRC structure can be considered an economical and sustainable alternative for future secondary structural parts in lightweight transport applications

Blade debitage in Southern Brazil: We have cores! [Debitagem laminar no Sul do Brasil: Habemus nucleos!]

Durante a pré-história, a debitagem laminar é um modo de produção característico de determinados períodos e regiões. No Brasil, no momento, foi reconhecida em três sítios arqueológicos situados no alto vale do rio Uruguai (SC/RS), em contextos sedimentares que datam do Holoceno inicial. Até hoje, somente os produtos dessa debitagem, as lâminas, tinham sido encontrados. A falta dos núcleos impedia um entendimento completo do processo de lascamento desses suportes. Graças à retomada das pesquisas na região desde 2013, foi possível coletar núcleos associados a essa produção laminar. Nesse artigo, descrevemos cinco desses núcleos. Suas análises demonstram que essa debitagem laminar corresponde a um único conceito. A estrutura volumétrica do núcleo que resulta desse conceito respeita as seguintes normas: 1) Os núcleos apresentam duas superfícies: um dorso plano e uma superfície de debitagem convexa mas relativamente achatada. Em uma extremidade, uma pequena superfície plana serviu de plano de percussão; 2) A debitagem começa por uma fase de inicialização durante a qual o plano de percussão é produzido por uma grande retirada. Em função das propriedades naturais do volume inicial, o dorso do núcleo é obtido, seja durante a seleção do bloco, seja por uma preparação por grandes retiradas transversais. Quando existem ainda, os negativos de retiradas da inicialização da superfície de debitagem indicam um método centrípeto; 3) As lâminas são sempre produzidas por um método unidirecional paralelo. A variabilidade desta debitagem é principalmente relacionada à técnica de lascamento: a percussão com pedra e a percussão com percutor orgânico foram utilizadas para a obtenção das lâminas. As informações tecnológicas fornecidas pelos núcleos aqui analisados são coerentes com essas observações oriundas da análise das lâminas. Com esses dois estudos consegue-se atingir um conhecimento completo desse sistema de produção do alto vale do rio Uruguai durante o Holoceno inicial, a única debitagem laminar documentada até hoje no Brasil.Blade debitage is a characteristic production pattern of specific periods and regions during Prehistory. In South America, it is well documented in Argentina, in contexts dating back to early Holocene. In Brazil, it was unknown until 2006, when it was found in three archaeological sites (ACH-LP-01, ACH-LP-03, ALP-AA-03) in the Foz do Chapecó area, upper Uruguay River, South Brazil, in layers from early Holocene. It was associated there with a great variety of other production systems: bifacial shaping of projectile points and large tools, unipolar debitage of large flakes, bipolar debitage of tiny flakes. Silicified sandstone, chalcedony and hyaline quartz are the main used raw materials. To this day, only the products of this debitage, the blades, had been discovered. Cores were lacking, which prevented a complete understanding of the flaking process. Due to the resumption of research in the region from 2013, it was possible to find those cores during surveys in the two following archaeological sites: ACH-LP-07 and RS-URG-01, located on both sides of the Uruguay river, near the mouth of the Chapecó river. We describe in this article five of these cores (4 from ACH-LP-07 and 1 from RS-URG-01). Their analyses demonstrate that this blade debitage complies with only one concept. The core volumetric structure that is the result of this concept displays the following features: 1) Cores have two surfaces: a flat back and a convex but relatively flattened flaking surface, so that they have a general “D” shaped transversal section. At one end, a small surface acts as striking platform during the production of the blades; 2) Debitage begins with a initialization phase of the core during which the striking platform is produced by a large removal. According to the natural properties of the initial volume, the back of the core is obtained either during the selection of the blank, and then it is left in his natural state, or by a preparation by one or more large transversal removals. When present, the scars of the initialization phase of the flaking surface indicate the use of a centripetal method. This preparation is made possible by the acute angle of the peripheral ridge formed by the intersection of the back and the flaking surface ; 3) Blades are always produced by a unidirectional parallel method. Production variability is mainly related to the flaking technique: both internal percussion by stone and marginal percussion by organic percussor were used to get the blades. Technical information provided by the cores are complementary and in accordance with those from the previous analyses of the blades from the same area. With these two studies it was possible to reach a relatively exhaustive understanding of this production system in the upper Uruguay River during early Holocene, the only well documented blade debitage in Brazil known until now. Future research will explore the interactions between this production and the other debitage and shaping methods inside this early Holocene technological system. To do this, we will develop a comprehensive study of all lithic artefacts from the archaeological layers in which blade production was identified. In a wider scale, a comparative approach with Argentinian and Uruguayan prehistoric blade productions will allow to better understand the development of the blade phenomenon in the southern cone of South America

Pathogenic diversity amongst serotype C VGIII and VGIV Cryptococcus gattii isolates

Cryptococcus gattii is one of the causative agents of human cryptococcosis. Highly virulent strains of serotype B C. gattii have been studied in detail, but little information is available on the pathogenic properties of serotype C isolates. In this study, we analyzed pathogenic determinants in three serotype C C. gattii isolates (106.97, ATCC 24066 and WM 779). Isolate ATCC 24066 (molecular type VGIII) differed from isolates WM 779 and 106.97 (both VGIV) in capsule dimensions, expression of CAP genes, chitooligomer distribution, and induction of host chitinase activity. Isolate WM 779 was more efficient than the others in producing pigments and all three isolates had distinct patterns of reactivity with antibodies to glucuronoxylomannan. This great phenotypic diversity reflected in differential pathogenicity. VGIV isolates WM 779 and 106.97 were similar in their ability to cause lethality and produced higher pulmonary fungal burden in a murine model of cryptococcosis, while isolate ATCC 24066 (VGIII) was unable to reach the brain and caused reduced lethality in intranasally infected mice. These results demonstrate a high diversity in the pathogenic potential of isolates of C. gattii belonging to the molecular types VGIII and VGIV

Chronotropic incompetence and a higher frequency of myocardial ischemia in exercise echocardiography

Background Exercise echocardiography (EE) is an established method to diagnose coronary artery disease (CAD). Chronotropic incompetence (CI) during the EE may be a marker of myocardial ischemia. The purpose of this investigation was to evaluate the additive value of CI during EE in CAD diagnosis. Methods Between 2000 and 2006, 4042 patients (1900 men with a mean age of 56 ± 11 years) were evaluated by EE. Based on the heart rate (HR) reached during the exercise test, the subjects were divided into two groups: G1 group – 490 patients who failed to achieve 85% of the maximal age-predicted HR, and G2 group – 3552 patients who were able to achieve 85% of the maximal age-predicted HR. Clinical characteristics, left ventricular wall motion abnormalities – wall motion score index (WMSI) – and coronary angiography (CA) were the parameters compared between the two groups. Results The left ventricular wall motion abnormalities were more frequent in G1 group than in G2 group (54% versus 26%; P < 0.00001). WMSI was higher in G1 group than in G2 group, both at rest (1.06 ± 0.17 versus 1.02 ± 0.09; P < 0.0001) and after exercise (1.12 ± 0.23 versus 1.04 ± 0.21; P < 0.0001). In G1 group, 82% of the patients with positive EE for myocardial ischemia presented obstructive coronary, compared to 71% (P = 0.03) in G2 group. Conclusion CI is associated with a higher frequency of myocardial ischemia during EE, reinforcing the concept that CI is a marker of the severity of myocardial ischemia