Discovery of widespread transcription initiation at microsatellites predictable by sequence-based deep neural network

Using the Cap Analysis of Gene Expression (CAGE) technology, the FANTOM5 consortium provided one of the most comprehensive maps of transcription start sites (TSSs) in several species. Strikingly, ~72% of them could not be assigned to a specific gene and initiate at unconventional regions, outside promoters or enhancers. Here, we probe these unassigned TSSs and show that, in all species studied, a significant fraction of CAGE peaks initiate at microsatellites, also called short tandem repeats (STRs). To confirm this transcription, we develop Cap Trap RNA-seq, a technology which combines cap trapping and long read MinION sequencing. We train sequence-based deep learning models able to predict CAGE signal at STRs with high accuracy. These models unveil the importance of STR surrounding sequences not only to distinguish STR classes, but also to predict the level of transcription initiation. Importantly, genetic variants linked to human diseases are preferentially found at STRs with high transcription initiation level, supporting the biological and clinical relevance of transcription initiation at STRs. Together, our results extend the repertoire of non-coding transcription associated with DNA tandem repeats and complexify STR polymorphism

Expedition to Alaid and Ebeko volcanoes (Kuril islands) in summer 2023

The results of field work at the active volcanoes Alaid and Ebeko, located in the northern part of the Kuril Island Arc, carried out in June−July 2023 are described. At Alaid volcano, the first survey of lava flows and fumarolic mineralization products of the 2022 eruption was carried out at various heights, including the summit, and geological sampling of the Taketomi cone and eruptive rocks of the eastern coast of Atlas Island was also conducted. An aerial photo and area temperature survey of the area occupied by the products of the 2022 eruption was undertaken at the summit of Alaid volcano using a high-precision quadrocopter equipped with a thermal imaging camera. At Ebeko volcano, mineral samples were taken from fumarole sites located on its eastern slope, and an aerial photo and area temperature survey were done

The mathematical model of characteristics of the convective unstable atmosphere taking into account microphysical processes in clouds

Предлагается математическая модель характеристик конвективно-неустойчивой атмосферы с учетом взаимосвязи термодинамических и микрофизических процессов, протекающих в облаках, позволяющая прогнозировать возникновение опасных явлений погоды. .// The new approach to obtaining diagnostic and prognostic information on characteristics of the atmosphere in the conditions of its convective instability based on mathematical model operation of the processes proceeding in conditions a cloud - and sludge formations and influencing dynamics of variability of meteorological fields is offered. Its application ensures increase in effectiveness and safety of functioning of organizational and technical systems

Mathematical model of stability of a timbering of a vertical mine trunk for ensuring ecological safety of production

Получены аналитические зависимости, моделирующие напряженно- деформированное состояние крепи вертикального шахтного ствола для материалов с пористой структурой, сжатый скелет которой обладает одновременно упругими и пластическими свойствами. В рамках точных трехмерных уравнений устойчивости исследована пространственная устойчивость основного состояния рассматриваемой цилиндрической крепи горной выработки. Реализация предлагаемых моделей обеспечивает повышение эффективности и экологической безопасности шахтных работ.//The analytical dependences modeling the intense deformed condition of a timbering of a vertical mine trunk for materials with porous structure which compressed skeleton has at the same time elastic and plastic properties are received. Within the exact three-dimensional equations of stability spatial stability of the main condition of the considered cylindrical timbering of an excavation is investigated. Realization of the offered models provides increase in efficiency and ecological safety of mine works