The boundary layer of VW Hyi in quiescence

In this letter, we suggest that the missing boundary layer luminosity of dwarf novae in quiescence is released mainly in the ultraviolet (UV) as the second component commonly identified in the far ultraviolet (FUV) as the "accretion belt". We present the well-studied SU UMa-type system VW Hyi in detail as a prototype for such a scenario. We consider detailed multiwavelength observations and in particular the recent FUSE observations of VW Hyi which confirm the presence of a second component (the "accretion belt") in the FUV spectrum of VW Hyi in quiescence. The temperature (50,000K) and rotational velocity (> 3,000km/s) of this second FUV component are entirely consistent with the optically thick region (tau = 1) located just at the outer edge of optically thin boundary layer in the simulations of Popham (1999). This second component contributes 20% of the FUV flux, therefore implying a boundary layer luminosity: $L_{BL} = 2 \times (0.2 \times L_{UV} + L_{X-ray}) = 0.6 \times L_{disc}$, while the theory (Klu\'zniak 1987) predicts, for the rotation rate of VW Hyi's WD, $L_{BL} \approx 0.77 L_{disc}$. The remaining accretion energy ($<0.1 L_{acc}$) is apparently advected into the star as expected for optically thin advection dominated boundary layers. This scenario is consistent with the recent simultaneous X-ray and UV observations of VW Hyi by (Pandel, C\'ordova & Howell 2003), from which we deduced here that the alpha viscosity parameter in the boundary layer region must be as small as $\alpha \approx 0.004$.Comment: 4 page

Photonic crystal resonator integrated in a microfluidic system

We report on a novel optofluidic system consisting of a silica-based 1D photonic crystal, integrated planar waveguides and electrically insulated fluidic channels. An array of pillars in a microfluidic channel designed for electrochromatography is used as a resonator for on-column label-free refractive index detection. The resonator was fabricated in a silicon oxynitride platform, to support electroosmotic flow, and operated at 1.55 microns. Different aqueous solutions of ethanol with refractive indices ranging from n = 1.3330 to 1.3616 were pumped into the column/resonator and the transmission spectra were recorded. Linear shifts of the resonant wavelengths yielded a maximum sensitivity of 480 nm/RIU and a minimum difference of 0.007 RIU was measured

Acoustic resonances in microfluidic chips: full-image micro-PIV experiments and numerical simulations

We show that full-image micro-PIV analysis in combination with images of transient particle motion is a powerful tool for experimental studies of acoustic radiation forces and acoustic streaming in microfluidic chambers under piezo-actuation in the MHz range. The measured steady-state motion of both large 5 um and small 1 um particles can be understood in terms of the acoustic eigenmodes or standing ultra-sound waves in the given experimental microsystems. This interpretation is supported by numerical solutions of the corresponding acoustic wave equation.Comment: RevTex, 10 pages, 9 eps figures; NOTE first authors changed his name to S. Melker Hagsater in the published versio

Electrocardiographic changes during continuous intravenous application of bupivacaine in neonatal pigs

Background It is controversial as to whether T-wave elevation is caused by local anaesthetics, epinephrine, or their combination. It has been shown that T-elevation after intravascular injection of a small bupivacaine test dose is caused by epinephrine and not by bupivacaine. The aim of this study was to investigate ECG changes with higher doses of i.v. bupivacaine. Methods Thirty neonatal pigs were anaesthetized with sevoflurane and their tracheas intubated and artificially ventilated. Under steady-state conditions, bupivacaine was continuously infused (flow rate 3.2 ml kg−1 min−1) by a syringe infusion pump through a central venous catheter. Group 1 received bupivacaine 0.125%, Group 2 bupivacaine 0.5%. The ECG was continuously printed and subsequently analysed for alterations in heart rate, ventricular de- and repolarization, and arrhythmias at 1.25, 2.5, and 5 mg kg−1 bupivacaine infused. Results Sinus rhythm persisted in all pigs. Heart rate decreased progressively in both groups, but this was significantly more pronounced in Group 1. T-wave elevation occurred in 40% and 0% (Groups 1 and 2) at 1.25 mg kg−1, in 80% and 0% at 2.5 mg kg−1, and in 93% and 80% at 5 mg kg−1 bupivacaine infused. There were significant differences between the two groups at 1.25 and 2.5 mg kg−1 infused. Conclusions Higher doses of i.v. infused bupivacaine can cause T-elevation. With slower injection technique, T-elevation can already be detected at lower bupivacaine doses administere

Integration of a zero dead-volume PDMS rotary switch valve in a miniaturised (bio)electroanalytical system

This work features the design, fabrication and characterisation of a miniaturised electroanalytical lab on a chip that allows the performance of a complete bioassay, from the capture of magnetic particles through their functionalisation and sample incubation to the detection of electroactive reaction products. The system is built using mainly polymeric materials such as PMMA and PDMS and fast prototyping techniques such as milling and moulding. The system also includes a set of microelectrodes, photo-lithographed on a silicon chip. The novelty lies in the design of the rotary microvalve, which contains a microreactor so that various reaction and incubation steps can be carried out in isolation from the detection event with zero dead volume. This avoids contamination and fouling of the electrodes by proteins or other organic matter, and extends the useful lifetime of the detector. The system operation is demonstrated by a model example, consisting in the functionalisation of streptavidin-coated magnetic particles with biotinylated β-galactosidase over periods ranging from 5 to 15 minutes, at which point the particles saturate. Although the system is intended for the development of enzyme-based electrochemical bioassays, the concept of its rotary microreactor can be applied more broadly.Peer reviewe

Electrocardiographic alterations during intravascular application of three different test doses of bupivacaine and epinephrine: experimental study in neonatal pigs

Background Origin of electrocardiographic (ECG) alterations during intravascular injection of local anaesthetic solutions is controversial. The aim of this study was to elucidate whether epinephrine, bupivacaine or their combination is responsible for ECG alteration. Methods Forty-five piglets were randomized into three groups. After induction of general anaesthesia using sevoflurane and peripheral venous cannulation, the trachea was intubated, the lungs were artificially ventilated, and anaesthesia was maintained by sevoflurane. Under steady state 0.2 ml kg−1 and after 10 min 0.4 ml kg−1 of one of the following three test solutions was administered i.v.: bupivacaine 0.125% (Group 1), bupivacaine 0.125%+epinephrine 1:200 000 (Group 2), and plain epinephrine 1:200 000 (Group 3). The ECG was analysed for alterations in heart rate and T-elevation. Results After injection of 0.2 or 0.4 ml kg−1 test solution, an increase in heart rate of at least 10% was found in none of Group 1 and in all of Groups 2 and 3. After application of 0.2 ml kg−1 test solution, T-elevation was found in 7% of Group 1 and in 93% of Groups 2 and 3. The injection of 0.4 ml kg−1 revealed a T-elevation in 27%, 100%, and 100%, respectively, in Groups 1, 2, and 3. Conclusions This animal model demonstrated that increases in heart rate and T-elevation in the ECG during i.v. application of a common test dose (0.2 ml kg−1) of bupivacaine are caused by epinephrine addition. Whether higher doses of bupivacaine alone can cause similar ECG changes or not requires further studie

Characterizing the pulsations of the ZZ Ceti star KUV 02464+3239

We present the results on period search and modeling of the cool DAV star KUV 02464+3239. Our observations resolved the multiperiodic pulsational behaviour of the star. In agreement with its position near the red edge of the DAV instability strip, it shows large amplitude, long period pulsation modes, and has a strongly non-sinusoidal light curve. We determined 6 frequencies as normal modes and revealed remarkable short-term amplitude variations. A rigorous test was performed for the possible source of amplitude variation: beating of modes, effect of noise, unresolved frequencies or rotational triplets. Among the best-fit models resulting from a grid search, we selected 3 that gave l=1 solutions for the largest amplitude modes. These models had masses of 0.645, 0.650 and 0.680 M_Sun. The 3 `favoured' models have M_H between 2.5x10^-5 - 6.3x10^-6 M_* and give 14.2 - 14.8 mas seismological parallax. The 0.645 M_Sun (11400 K) model also matches the spectroscopic log g and T_eff within 1 sigma. We investigated the possibility of mode trapping and concluded that while it can explain high amplitude modes, it is not required.Comment: 11 pages, 8 figures, accepted for publication in MNRA